• Journal of Microbiology and Biotechnology
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• 제33권7호
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• pp.973-979
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• 2023

Lycopene is a carotenoid widely used as a food and feed supplement due to its antioxidant, anti-inflammatory, and anti-cancer functions. Various metabolic engineering strategies have been implemented for high lycopene production in Escherichia coli, and for this purpose it was essential to select and develop an E. coli strain with the highest potency. In this study, we evaluated 16 E. coli strains to determine the best lycopene production host by introducing a lycopene biosynthetic pathway (crtE, crtB, and crtI genes cloned from Deinococcus wulumuqiensis R12 and dxs, dxr, ispA, and idi genes cloned from E. coli). The 16 lycopene strain titers diverged from 0 to 0.141 g/l, with MG1655 demonstrating the highest titer (0.141 g/l), while the SURE and W strains expressed the lowest (0 g/l) in an LB medium. When a 2 × YTg medium replaced the MG1655 culture medium, the titer further escalated to 1.595 g/l. These results substantiate that strain selection is vital in metabolic engineering, and further, that MG1655 is a potent host for producing lycopene and other carotenoids with the same lycopene biosynthetic pathway.

• 근관절건강학회지
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• 제25권2호
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• pp.112-121
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• 2018

Purpose: This study was conducted to explore the mediating effect of nutritional status on the relationship between symptom experience and functional status of patients with Chronic Obstructive Pulmonary Disease (COPD). Methods: A total of 141 COPD patients visiting D hospital and I hospital in B city were enrolled in this study. Data were collected from January 2017 to July 2017. Outcome variables were measured by Mini Nutritional Assessment Short-Form (MNA-SF) for nutritional status, The Memorial Symptom Assessment Scale (MSAS) for symptom experience, and The Functional Performance Inventory Short Form (FPI-SF) for functional status. The data were analyzed with descriptive statistics, Pearson's correlation, and path analysis using SPSS/WIN 21.0 and AMOS 25. Results: There was a significant negative correlation between symptom experience and nutritional status (r=-.61, p<.001), a significant negative correlation between symptom experience and functional status (r=-.40, p<.001), and significant positive correlation between nutritional status and functional status (r=.47, p<.001). Symptom experience had indirect effects on functional status through nutritional status. Conclusion: For enhancing functional status in COPD patients, it is necessary to develop nursing intervention programs to enhance symptom management as well as nutritional status.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.116-116
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• 2018

Bioconversion, the enzymatic process by microorganism on organic precursor to desired products. The natural extract is converted into a form that can be easily absorbed into the skin, while scaling up of to higher quantity is possible. Selection of naturally processed raw material rather than chemically processed is preferred. Therefore, fermentation was carried out by mixing Rubus coreanus Miquel, soybean, Zanthoxylum schinifolium as bioconverting materials, the possibility of inflammation, whitening material were checked. In this study, useful microorganisms were isolated from various salted fish, and 16S rDNA sequence was analyzed to confirm their genetic characteristics. Combining the three natural materials using bioconversion technology to study their activity before and after fermentation. To evaluate the antioxidant activity and the active ingredient content the DPPH radical scavenging activity and the total polyphenol content were examined. Raw 264.7 cells were used to evaluate MTT assay, NO and $TNF-{\alpha}$ production inhibitory activity. Also, to evaluate the whitening activity, tyrosinase inhibitory activity and melanin formation inhibitory activity were measured using B16F10 cells. In total 34 strains were obtained from various salted fish. The effective strains useful for the bioconversion process, showed that DPPH radical scavenging ability and polyphenol content were increased in the two kinds of microbial treatment groups compared to the untreated group. 16S rDNA sequencing analysis of the strains showed excellent in Pediococcus pentosaceus B1 in comparison. An increase of up-to 156% in anti-oxidative activity and 141% in polyphenol content was observed after bioconversion. In addition, after mixed fermentation the toxidty of Raw 264.7 and B16F10 cells tended to decrease and a significant increase was observed in anti-inflammatory activity as well. Also, tyrosinase activity and melanin significantly. synthesis decreased significantly.

• 한국수정란이식학회지
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• 제20권1호
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• pp.49-53
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• 2005

다양한 발정유기 방법의 반복처리에 따른 분만율은 평균 $90.7\%(236/260)$로 나타났고 생시체중에 있어서 자연 발정구와 발정 유기구에서 차이를 거의 보이지 않았으며 송아지 육성율은 농가의 사육경험이 많을수록 우수한 경향이었고 발정 동기화의 반복처리가 차기번식에 미치는 영향은 확인할 수 없었다. 1. 발정 유기구와 자연 발정구에서 출생한 송아지의 생시체중은 암송아지에서 각각 23.9kg, 24.0kg이었고, 수송아지에서 26.2kg, 24.9kg로써 처리간 차이가 없었다. 2. 산차에 따른 생시체중은 초산에서 23.4kg, 2산에서 25.3kg, 3산에서 26.2kg으로 유의적으로 증가하는 경향이었으나, 5산 이후에서는 비슷하였다. 3. 분만후 발정재귀일수는 대조구에서 80.7일, $PGF_{2}{\alpha}$구에서 92.3일, PRID구에서 78.5일, CIDR구에서 64.5일, $GnRH-PGF\_2{\alpha}-GnRH$ 처리구에서 65.6일로 나타났다. 4. 분만후 수태일수는 대조구에서 137.1일, $PGF_{2}{\alpha}$구에서 147.6일, PRID구에서 141.3일, CIDR구에서 116.6일, $GnRH-PGF\_{2}{\alpha}-GnRH$처리구에서 118일로 나타났다.

• JSTS:Journal of Semiconductor Technology and Science
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• 제4권3호
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• pp.141-148
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• 2004

We discuss and develop a compact MOSFET modeling scheme in order to obtain accurate descriptions of the drain current and its derivatives up to the 5th order. We have analyzed the physical effects which govern the behaviour of the 3rd derivative in long and deep-subrnicron channel MOSFETs. Our modeling agrees well with experimental data and describes continuous transitions between operating regimes, thanks to the use of continuous functions, which do not introduce any artificial peaks.

• 산업기술연구
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• 제25권B호
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• pp.141-147
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• 2005

A target of this paper is to get some elementary experimental data on the energy harvesting using a piezoelectric material. A THUNDER series piezo material (TH7-R), which has been developed by NASA engineer is selected for this study. In order to provide a mechanical energy to the piezoelectric material, a mechanical motion vibrator and its driving electronics are designed. Using a simple PWM control, the excitation frequency of vibrating mechanical motion is varied. The generated electric power as a function of the excitation frequency is monitored and analyzed. This initial experiment shows a possible energy source using a piezoelectric material for the application to low-power consumed small electronic devices such as RFID, MEMS, and etc.

• 설비공학논문집
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• 제12권9호
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• pp.853-859
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• 2000

In this study, low temperature waste heat recovery heat exchanger was developed using a principle of self-excited oscillating heat pipe. The heat exchanger of serpentine type was composed of extruded flat aluminum tube with 6 channels (3 nm$\times$ 2.75nm) and louvered fin. The heat transfer area density of heat exchanger was $331.9 m^2/m^3$. Working fluid is R141b and charge ratio was 40% by volume. Heat transfer rate and the effectiveness of heat exchanger was primary concern of this study. As a result, the effectiveness of heat exchanger was about 0.4-0.67, and recovered waste heat rate was about 4.5 kW per one unit of heat exchanger.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2003년도 춘계학술대회
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• pp.1499-1505
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• 2003

Rapid development of electronic technology requires small size, high density packaging and high power of electronic devices. So, in this paper, characteristics on oscillating heat pipe according to operating conditions(environment temperature, charging ratio of working fluid, inclination) based on experimental study was investigated. From the experimental results, $25^{\circ}C$environment temperature), R-141b(working fluid)40%(charging ratio) was best performace at others of inclination angle and The top heating mode of OCHP performed 80% efficiency of the bottom heating mode.

• 설비공학논문집
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• 제6권3호
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• pp.247-258
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• 1994

In this study, experimental investigations to find cold energy storage performance have been made for two different temperatures at condenser. Temperatures at inlet and outlet of condenser were measured to calculate global heat transfer coefficient of direct contact method in our cold energy storage system. Also storage performance by direct contact method was compared with that of Ice-On-Coil type ice storage which was calculated by analytic solution. Results show that, in the case of $-8.0^{\circ}C$ at condenser inlet, heat transfer coefficient of direct contact method is 3.25 times higher than that of conventional method and COP of system is improved by using R141b as refrigerant which produces gas hydrate and has higher phase change temperature than $0.0^{\circ}C$.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2007년도 추계학술대회 논문집
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• pp.139-141
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• 2007

Compression tests were carried out to investigate morphologies of compressed specimen, deformation microstructure and stress-strain relation in high-nitrogen austenite stainless steel. Tests were performed under a wide range of temperature and, with true strain rates up to $\dot{\varepsilon}$ =0.05, 0.1, 0.5 and $1.0s^{-1}$. The activation energy of loading force was equal to plastic deformation energy within the temperature range of $900^{\circ}C$ to $1250^{\circ}C$. Dynamically recrystallized grain size decreased with an increasing strain rate and temperature. Flow stresses and deformation microstructures, were used to quantify the critical strain rate and recrystallized grain size. The grain size versus strain rate-temperature map obtained in the study was in good agreement with the deformation microstructures of compressed specimens.