• 제목/요약/키워드: R&D Lab.

검색결과 1,018건 처리시간 0.029초

N-glycoproteomic analysis of human follicular fluid during natural and stimulated cycles in patients undergoing in vitro fertilization

  • Lim, Hee-Joung;Seok, Ae Eun;Han, Jiyou;Lee, Jiyeong;Lee, Sungeun;Kang, Hee-Gyoo;Cha, Byung Heun;Yang, Yunseok
    • Clinical and Experimental Reproductive Medicine
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    • 제44권2호
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    • pp.63-72
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    • 2017
  • Objective: Hyperstimulation methods are broadly used for in vitro fertilization (IVF) in patients with infertility; however, the side effects associated with these therapies, such as ovarian hyperstimulation syndrome (OHSS), have not been well studied. N-glycoproteomes are subproteomes used for the remote sensing of ovarian stimulation in follicular growth. Glycoproteomic variation in human follicular fluid (hFF) has not been evaluated. In this study, we aimed to identify and quantify the glycoproteomes and N-glycoproteins (N-GPs) in natural and stimulated hFF using label-free nano-liquid chromatography/electrospray ionization-quad time-of-flight mass spectrometry. Methods: For profiling of the total proteome and glycoproteome, pooled protein samples from natural and stimulated hFF samples were selectively isolated using hydrazide chemistry to obtain the total proteomes and glycoproteomes. N-GPs were validated by the consensus sequence N-X-S/T (92.2% specificity for the N-glycomotif at p<0.05). All data were compared between natural versus hyperstimulated hFF samples. Results: We detected 41 and 44 N-GPs in the natural and stimulated hFF samples, respectively. Importantly, we identified 11 N-GPs with greater than two-fold upregulation in stimulated hFF samples compared to natural hFF samples. We also validated the novel N-GPs thyroxine-binding globulin, vitamin D-binding protein, and complement proteins C3 and C9. Conclusion: We identified and classified N-GPs in hFF to improve our understanding of follicular physiology in patients requiring assisted reproduction. Our results provided important insights into the prevention of hyperstimulation side effects, such as OHSS.

갯장어껍질 열수 추출물의 항산화 효과 (Antioxidant Effect of HotWaterExtract of Muraenesox cinereus's Skin)

  • 신현재;김윤수;남형근;나명순;김미혜;강형봉;이희덕;최두복;최온유;차월석
    • KSBB Journal
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    • 제26권1호
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    • pp.27-32
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    • 2011
  • For the development of functional food and cosmetics using hot water extract of Muraenesox cinereus's skin, contents of vitamin, amino acid and element, and antioxidant activity were investigated. The results are shown as follows: among vitamins, A(0.21mg/100 g), C (78.12mg/100 g), $D_3$ (0.03 mg/100 g), E (1.97 mg/100 g) and Niacin (2.53mg/100 g) were detected, respectively.Mineral contents were an order of K > P > Na > Mg > Ca > Fe and Zn. Contents of total amino acids were an order of Pro > Gly > Arg > Glu > Phe > Ala. Especially, the sum of total amino acids was 27.17 mg/100 mL, which was about 4.0 fold higher than that of free amino acid. DPPH radical scavenging activity of hot water extract of M. cinereus's skin at 25 mg/mL was 63.5% and did not increase at above 50 mg/mL. Activities of antioxidant enzymes in the liver of ethanol-treated rats using hot water extract of M. cinereus's skin were investigated. Compared to control group, activities of ADH and GSH-px were decreased. In the case of CAT and SOD activity, they were increased. These results showed that the hot water extract of M. cinereus's skin can be applied to raw macterial for functional food and cosmetics.

PASTELS project - overall progress of the project on experimental and numerical activities on passive safety systems

  • Michael Montout;Christophe Herer;Joonas Telkka
    • Nuclear Engineering and Technology
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    • 제56권3호
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    • pp.803-811
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    • 2024
  • Nuclear accidents such as Fukushima Daiichi have highlighted the potential of passive safety systems to replace or complement active safety systems as part of the overall prevention and/or mitigation strategies. In addition, passive systems are key features of Small Modular Reactors (SMRs), for which they are becoming almost unavoidable and are part of the basic design of many reactors available in today's nuclear market. Nevertheless, their potential to significantly increase the safety of nuclear power plants still needs to be strengthened, in particular the ability of computer codes to determine their performance and reliability in industrial applications and support the safety demonstration. The PASTELS project (September 2020-February 2024), funded by the European Commission "Euratom H2020" programme, is devoted to the study of passive systems relying on natural circulation. The project focuses on two types, namely the SAfety COndenser (SACO) for the evacuation of the core residual power and the Containment Wall Condenser (CWC) for the reduction of heat and pressure in the containment vessel in case of accident. A specific design for each of these systems is being investigated in the project. Firstly, a straight vertical pool type of SACO has been implemented on the Framatome's PKL loop at Erlangen. It represents a tube bundle type heat exchanger that transfers heat from the secondary circuit to the water pool in which it is immersed by condensing the vapour generated in the steam generator. Secondly, the project relies on the CWC installed on the PASI test loop at LUT University in Finland. This facility reproduces the thermal-hydraulic behaviour of a Passive Containment Cooling System (PCCS) mainly composed of a CWC, a heat exchanger in the containment vessel connected to a water tank at atmospheric pressure outside the vessel which represents the ultimate heat sink. Several activities are carried out within the framework of the project. Different tests are conducted on these integral test facilities to produce new and relevant experimental data allowing to better characterize the physical behaviours and the performances of these systems for various thermo-hydraulic conditions. These test programmes are simulated by different codes acting at different scales, mainly system and CFD codes. New "system/CFD" coupling approaches are also considered to evaluate their potential to benefit both from the accuracy of CFD in regions where local 3D effects are dominant and system codes whose computational speed, robustness and general level of physical validation are particularly appreciated in industrial studies. In parallel, the project includes the study of single and two-phase natural circulation loops through a bibliographical study and the simulations of the PERSEO and HERO-2 experimental facilities. After a synthetic presentation of the project and its objectives, this article provides the reader with findings related to the physical analysis of the test results obtained on the PKL and PASI installations as well an overall evaluation of the capability of the different numerical tools to simulate passive systems.

특정 대상에 대한 개인 수준의 문화적 성향이 사용자 경험에 미치는 조절효과에 대한 실증적 연구: 미국, 독일, 러시아의 4개 디지털 기기 사용자를 대상으로 (An Empirical Study on How the Moderating Effects of Individual Cultural Characteristics towards a Specific Target Affects User Experience: Based on the Survey Results of Four Types of Digital Device Users in the US, Germany, and Russia)

  • 이인성;최지웅;김소령;이기호;김진우
    • Asia pacific journal of information systems
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    • 제19권1호
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    • pp.113-145
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    • 2009
  • Recently, due to the globalization of the IT(Information Technology) market, devices and systems designed in one country are used in other countries as well. This phenomenon is becoming the key factor for increased interest on cross-cultural, or cross-national, research within the IT area. However, as the IT market is becoming bigger and more globalized, a great number of IT practitioners are having difficulty in designing and developing devices or systems which can provide optimal experience. This is because not only tangible factors such as language and a country's economic or industrial power affect the user experience of a certain device or system but also invisible and intangible factors as well. Among such invisible and intangible factors, the cultural characteristics of users from different countries may affect the user experience of certain devices or systems because cultural characteristics affect how they understand and interpret the devices or systems. In other words, when users evaluate the quality of overall user experience, the cultural characteristics of each user act as a perceptual lens that leads the user to focus on a certain elements of experience. Therefore, there is a need within the IT field to consider cultural characteristics when designing or developing certain devices or systems and plan a strategy for localization. In such an environment, existing IS studies identify the culture with the country, emphasize the importance of culture in a national level perspective, and hypothesize that users within the same country have same cultural characteristics. Under such assumptions, these studies focus on the moderating effects of cultural characteristics on a national level within a certain theoretical framework. This has already been suggested by cross-cultural studies conducted by scholars such as Hofstede(1980) in providing numerical research results and measurement items for cultural characteristics and using such results or items as they increase the efficiency of studies. However, such national level culture has its limitations in forecasting and explaining individual-level behaviors such as voluntary device or system usage. This is because individual cultural characteristics are the outcome of not only the national culture but also the culture of a race, company, local area, family, and other groups that are formulated through interaction within the group. Therefore, national or nationally dominant cultural characteristics may have its limitations in forecasting and explaining the cultural characteristics of an individual. Moreover, past studies in psychology suggest a possibility that there exist different cultural characteristics within a single individual depending on the subject being measured or its context. For example, in relation to individual vs. collective characteristics, which is one of the major cultural characteristics, an individual may show collectivistic characteristics when he or she is with family or friends but show individualistic characteristics in his or her workplace. Therefore, this study acknowledged such limitations of past studies and conducted a research within the framework of 'theoretically integrated model of user satisfaction and emotional attachment', which was developed through a former study, on how the effects of different experience elements on emotional attachment or user satisfaction are differentiated depending on the individual cultural characteristics related to a system or device usage. In order to do this, this study hypothesized the moderating effects of four cultural dimensions (uncertainty avoidance, individualism vs, collectivism, masculinity vs. femininity, and power distance) as suggested by Hofstede(1980) within the theoretically integrated model of emotional attachment and user satisfaction. Statistical tests were then implemented on these moderating effects through conducting surveys with users of four digital devices (mobile phone, MP3 player, LCD TV, and refrigerator) in three countries (US, Germany, and Russia). In order to explain and forecast the behavior of personal device or system users, individual cultural characteristics must be measured, and depending on the target device or system, measurements must be measured independently. Through this suggestion, this study hopes to provide new and useful perspectives for future IS research.

어피 펩타이드 리포좀 대량생산 최적 조건 및 저장 안정성 (Optimization and Scale-up of Fish Skin Peptide Loaded Liposome Preparation and Its Storage Stability)

  • 이중규;이윤정;백정정;김수진;조영재;최미정
    • 산업식품공학
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    • 제21권4호
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    • pp.360-366
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    • 2017
  • 본 연구에서는 lab scale 용량으로 100 mL, 1 L 단위로 리포좀을 제조하였으며, prototype scale로서 10 L 단위로 blank 리포좀의 제조한 뒤 입자 크기 및 포집 효율을 측정하여 최적 제조 조건 선정하였다. 선정한 최적조건으로 어피 펩타이드를 리포좀으로 포집하여 그에 따른 저장 안정성을 평가하였다. 1차 균질 조건은 초고속균질기를 사용하여 3분간 각각 4,000 rpm, 8,000 rpm, 12,000 rpm으로 균질하였으며, 2차 균질 조건은 초음파균질기를 이용하여 각각 40 W, 60 W, 80 W로 3분간 균질하여 최적 균질 조건을 확립한 뒤 어피 펩타이드 리포좀을 농도에 따라 제조하여 $4^{\circ}C$에서 냉장 저장하였다. 최적 제조 조건 실험 결과를 two-way ANOVA로 분석한 결과 1, 2차 균질에서는 제조 용량이 입자크기와 제타 전위에 유의적으로 가장 큰 영향을 미쳤으며(p<0.001), pdI는 2차 균질 조건에서 제조용량을 제외하고는 어떤 요인도 유의적으로 영향을 미치지 못하였다(p>0.05). 1차 균질 실험 결과 lab scale에서 prototype scale로 제조 용량이 증가하였을 때, 유의적으로 입자크기가 증가하였으며(p<0.05), 가장 입자크기가 작고 제타전위의 절대값이 높은 4,000 rpm을 최적조건으로 선정하였다. 2차 균질 실험결과 40 W에서 제조 용량이 증가하였을 때 유의적으로 제타전위가 감소하였으며, 60 W 이상에서는 안정적인 결과가 나타났다. 리포좀의 산업적 적용을 고려할 때 공정비용 감소 측면에서 80 W보다 60 W가 적절하다고 사료된다. 선정된 리포좀 최적 조건으로 농도(3, 6, 12, 24%)별로 어피 펩타이드를 포집하였을 때, 24%에서 입자크기가 1 mm 이상으로 크게 나타났다. 이후 저장실험에서는 24%를 제외한 3가지 조건으로 진행하였다. 1달간 어피 펩타이드 리포좀을 냉장 저장한 결과를 two-way ANOVA로 분석한 결과, 펩타이드 농도에 따라 제조하였을 때는 입자크기와 제타 전위가 제조용량보다 펩타이드 농도에 더 큰 영향을 받았다. 또한 저장기간은 pdI에 유의적으로 영향을 미치는 것으로 나타났다(p<0.001). 입자크기는 제조용량과 펩타이드 농도가 증가함에 따라서 유의적으로 증가하였으며, 저장기간에 따라서 감소하였다. 제타전위는 10 L 용량에서 저장기간에 따라 증가하는 경향을 보였다. 이는 리포좀이 풀리면서 비표면적이 증가하여 제타전위가 증가한 것으로 사료된다. 또한 12%에서 3주차부터 침전물층이 형성되는 것이 관찰되어 6%가 가장 산업적용으로 적합한 농도로 사료된다.

HPLC-UVD를 이용한 농산물 중 스피노사드 분석법 개선 및 잔류실태 조사 (Development of Analytical Methods of Spinosad in Agricultural Commodities by HPLC with UV Detector and Monitoring)

  • 김희연;최선희;정소영;최희주;김용훈;조민자;서은채;한경진;최재천;박희옥;하상철;신일식;엄지윤
    • 한국식품과학회지
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    • 제43권2호
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    • pp.125-133
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    • 2011
  • 본 연구는 단성분 농약 중 스피노사드의 잔류실태를 조사하기 위하여 식품공전의 스피노사드 분석법을 농산물에 적용되도록 개선하고 개선 된 시험법으로 잔류실태조사가 부족한 스피노사드에 대한 잔류실태를 조사하였다. 국내 유통 중인 16종의 농산물 304건에 대한 스피노사드 잔류량을 조사한 결과 모든 검체에서 검량한계 이상 검출 되지 않았다. 스피노사드 시험법의 회수율은 현미, 강낭콩, 오렌지, 들깻잎, 표고버섯에서 스피노사드 A의 경우 0.05 mg/kg 수준에 각각 76.2, 95.3, 83.5, 79.9, 98.6%이고, 0.25 mg/kg 수준에 각각 97.3, 86.6, 84.4, 94.0, 88.1%이고, 스피노사드 D 의 경우 0.05 mg/kg 수준에 각각 76.6, 104.0, 88.9, 74.9, 81.1%이고, 0.25 mg/kg 수준에 각각 92.7, 97.7, 86.9, 90.4, 85.4%였으며 검출한계 0.001 mg/kg, 검량한계 0.005 mg/kg이다. 회수율에 대한 모든 표준편차는 10% 미만이다. 스피노사드 A와 D는 0.1-5.0 mg/kg 범위에서 상관계수($r^2$) 0.9999 이상의 높은 직선성을 보이고, 검출한계는 0.001 mg/kg, 검량한계는 0.005 mg/kg이었다.

비디오 통신 시스템의 실시간 비디오 품질 측정 방법 (Real-Time Video Quality Assessment of Video Communication Systems)

  • 김병용;이선오;정광수;심동규;이수연
    • 대한전자공학회논문지SP
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    • 제46권3호
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    • pp.75-88
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    • 2009
  • 본 논문에서는 실시간 멀티미디어 스트림 서비스에서 발생하는 비디오 품질 저하 요인에 따른 비디오 품질 측정 방법을 제안한다. 비디오 품질 저하에는 대표적으로 비디오 압축과 네트워크의 상태에 의해 발생한다. 본 논문에서는 비디오 압축에 의하여 생기는 화질 저하를 측정하기 위하여 공간 도메인에서 블록킹 왜곡에 대한 정도를 측정하는 방법을 제안한다. 제안하는 블록 경계 강도는 $8{\times}8$ 블록 경계에서 픽셀간의 변화와 경계를 제외한 주변영역에서 픽셀 사이의 변화에 대한 비율로 나타내었다. 반면, 움직임이 부자연스러운 동영상은 지터 및 지연 요소와 같은 네트워크 전송능력의 악화로 인해 비디오 품질 저하가 나타난다. 본 논문에서 제안하는 시간적 저키니스는 연속된 프레임의 대응되는 픽셀간의 밝기 값 차이에 대한 평균과 현재 프레임과 이전 프레임의 재생시간 간격의 평균 및 분산을 이용하는 방법을 제안하였다. 또한, 본 논문에서는 비디오 압축에 의한 품질 측정 방법인 블록 경계 강도와 네트워크에 의한 품질 측정 방법인 시간적 저키니스를 통합하여 비디오의 종합적 품질을 평가하는 Perceptual Video Quality Metric (PVQM)을 제안한다. 제안한 알고리즘의 성능을 평가하기 위하여, 사람이 직접 비디오 품질을 평가하는 Difference of Mean Opinion Score (DMOS)와 제안한 알고리즘을 비교 평가하였다. 그 결과 제안한 PVQM의 결과와 인간 시각에 의하여 평가된 DMOS가 근사함을 확인하였다.

역산이론을 이용한 연안 수질모형의 매개변수 추정 (Parameter Estimation of Coastal Water Quality Model Using the Inverse Theory)

  • 조홍연;조범준;정신택
    • 한국해안해양공학회지
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    • 제17권3호
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    • pp.149-157
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    • 2005
  • 모형의 지배방정식에서 정의되는 대표적인 매개변수는 유역 및 대기로부터의 오염부하량,퇴적물로부터의 오염물질 용출부하량, 확산계수, 반응계수 등으로, 직접적인 관측이 곤란할 뿐만 아니라 많은 관측비용을 필요로 한다. 본 연구에서는 매개변수를 포함한 오염물질 수지방정식을 구성하고, 구성된 선형 연립방정식을 이용하여 계산된 농도분포자료와 관측된 시계열 농도턱포자료를 이용하여 계산한 질량변화량의 차이를 최소화하는 역산문제를 구성하여 모형의 매개변수를 추정하는 방법을 제시하였다. 이 방법을 이용하여 천수만, 울산만(울산항)해 역에서 관측된 연직방향 농도분포 자료를 이용하여 연직 확산계수 및 대기로부터의 오염부하량, 퇴적물로부터의 오염물질 용출부하량, 확산$\cdot$반응에 의한 오염물질 변화량 등을 추정하였으며, 추정 매개변수는 시기적으로 변동이 크게 나타났다. 한편, 관측자료와 추정매개변수를 이용한 계산결과를 비교한 결과, RMS 오차는 괄측자료 범위의 $5.0\%$ 이하, 일치지수는 0.95 이상으로 본 방법을 이용한 매개변수 추정결과의 신뢰성은 우수한 것으로 파악되었다.

가축 사체 매몰지 침출수 처리를 위한 Fenton 산화공정의 최적조건 (Optimal Condition of Operation Parameter for Livestock Carcass Leachate using Fenton Oxidation Process)

  • 안상우;정영철;유지영;민지은;이시진;박재우
    • 한국지하수토양환경학회지:지하수토양환경
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    • 제18권1호
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    • pp.26-35
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    • 2013
  • Outbreak of animal infectious diseases such as foot-and-mouth disease, avian influenza are becoming prevalent worldwide. For prevent the further infection, tremendous numbers of the infected or culled stocks are buried around farm. This burial method can generate a wide range of detrimental components such as leachate, nutrient, salt, and pathogenic bacteria, consequently. In this study, for the stabilization of livestock carcasses leachate, advanced oxidation processes utilizing the Fenton reaction was investigated in lab-scale experiments for the treatment for $COD_{Cr}$ of livestock carcass leachate. $COD_{Cr}$ reduction by the Fenton oxidation was investigated response surface methodology using the Box-Begnken methods were applied to the experimental results. A central composite design was used to investigate the effects of the independent variables of pH ($x_1$), dosage of $FeCl_2{\cdot}4H_2O$ ($x_2$) and dosage of $H_2O_2$ ($x_3$) on the dependent variables $COD_{Cr}$ concentration ($y_1$). A 1 M NaOH and $H_2SO_4$ was using for pH control, $FeCl_2{\cdot}4H_2O$ was used as iron catalyst and NaOH was used for Fenton reaction. The optimal conditions for Fenton oxidation process were determined: pH, dosage of $FeCl_2{\cdot}4H_2O$ and dosage of $H_2O_2$ were 3, 0.6 g (0.0151 M) and 7 mL(0.259 M), respectively. Statistical results showed the order of significance of the independent variables to be pH > initial concentration of ferrous ion > initial concentration of hydrogen peroxide.

표면의 이슬 맺힘 저감을 위한 냉장고 가스켓 및 냉동냉장실 내벽 구조개선에 관한 연구 (A Study on the Design Parameters of a Gasket and Innercase of a Refrigerator to Reduce Dew Generation on the Outer Surface)

  • 강석훈;김성진;김주환;민준기;손창민;박상후
    • 대한기계학회논문집B
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    • 제36권4호
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    • pp.457-463
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    • 2012
  • 최근 사용자의 편의성과 냉장고 저장 공간의 확보를 위해 냉장고 문과 내벽을 얇게 제작하고 있다. 따라서 냉기차단 벽두께의 감소로 인하여 내 외부 온도차가 일정수준 이상으로 발생하고 이에 따라 표면의 이슬 맺힘 현상이 문제가 되는데, 이는 냉장고 설계에 있어 해결되어야 할 중요한 문제이다. 문제를 해결하기 위해 지금은 일반적으로 전기히터와 같은 발열체를 사용하였는데, 이 경우에 불필요한 전력소모가 뒤따른다. 본 연구에서는 기존방식과 달리 전기를 사용하지 않고 기계적 구조변경을 통한 해결방안으로 가스켓 최적화와 냉동냉장실 내벽두께 조정을 통해 표면에 이슬 맺힘 현상을 저감하는 방법을 제안한다. 해석결과를 살펴보면, 히터의 사용 없이 이슬 맺힘이 발생하는 부분에서의 온도가 국부적으로 $0.39{\sim}3.07^{\circ}C$까지 상승하는 효과를 얻어서 이슬 맺힘 현상을 저감시켰다.