• Proceedings of the Korean Information Science Society Conference
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• 2006.10a
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• pp.459-463
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• 2006

유비쿼터스 환경은 이질적인 디바이스와 서비스로 구성되어 있으며 많은 컴포넌트들이 각기 목적을 가지고 여러 네트워크에 산재되어있다. 이러한 디바이스들을 연결하고 서비스를 효율적으로 관리하기 위해 비슷한 목적을 가지는 컴포넌트들을 하나의 그룹으로 묶을 수 있다. 이러한 그룹의 멤버들은 서비스를 제공하기 위해 같은 그룹의 멤버들과 리소스를 공유하며 때로는 그룹의 모든 멤버들이 동시에 하나의 리소스를 사용하기도 한다. 서로 다른 그룹이 이러한 리소스를 공유하기도 한다. 따라서 그룹 간에 상호 배제 알고리즘이 필요하다. 이 논문에서는 쿼럼-기반 알고리즘(Quorum-based algorithm)을 응용하여 유비쿼터스 환경에서 그룹간의 상호 배제문제를 해결하는 알고리즘을 제안하고 이에 대한 성능측정 결과를 설명한다.

• Journal of the Korean Institute of Intelligent Systems
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• v.18 no.3
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• pp.392-400
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• 2008

This paper proposes a novel optimization algorithm inspired by bacteria behavior patterns for foraging. Most bacteria can trace attractant chemical molecules for foraging. This tracing capability of bacteria called chemotaxis might be optimized for foraging because it has been evolved for few millenniums. From this observation, we developed a new optimization algorithm based on the chemotaxis of bacteria in this paper. We first define behavior and decision rules based on the behavior patterns of bacteria and then devise an optimization algorithm with these behavior and decision rules. Generally bacteria have a quorum sensing mechanism that makes it possible to effectively forage, but we leave its implementation as a further work for simplicity. Thereby, we call our algorithm a simple bacteria cooperative optimization (BCO) algorithm. Our simple BCO is tested with four function optimization problems on various' parameters of the algorithm. It was found from experiments that the simple BCO can be a good framework for optimization.

• Journal of Microbiology and Biotechnology
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• v.29 no.9
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• pp.1341-1348
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• 2019

Acetic acid is indirectly involved in cell center metabolism, and acetic acid metabolism is the core of central metabolism, affecting and regulating the production of bacteriocin. Bacteriocin is a natural food preservative that has been used in the meat and dairy industries and winemaking. In this paper, the effects of acetic acid on bacteriocin produced by Gram-positive bacteria were reviewed. It was found that acetic acid in the undissociated state can diffuse freely through the hydrophobic layer of the membrane and dissociate, affecting the production, yield, and activity of bacteriocin. In particular, the effect of acetic acid on cell membranes is summarized. The link between acetic acid metabolism, quorum sensing, and bacteriocin production mechanisms is also highlighted.

• International Journal of Computer Science & Network Security
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• v.22 no.6
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• pp.163-171
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• 2022

Online services are widely spread, and their use increases day by day. As COVID-19 spread and people spent much time online, fraud scams have risen unexpectedly. Manipulation techniques have become more effective at swindling those lacking basic technological knowledge. Unfortunately, a user needs a quorum. The interest in preventing scammers from obtaining effective quality service has become the most significant obstacle, increasing the variety of daily Internet platforms. This paper is concerned with analyzing purchase data and extracting provided results. In addition, after examining relevant documents presenting research discussing them, the recommendation was made that future work avoids them; this would save a lot of effort, money, and time. This research highlights many problems a person may face in dealing with online institutions and possible solutions to the epidemic through theft operations on the Internet.

• Journal of Microbiology and Biotechnology
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• v.30 no.7
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• pp.1108-1108
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• 2020

• Proceedings of the Korea Information Processing Society Conference
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• 2021.11a
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• pp.334-336
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• 2021

허가형 블록체인 기반 데이터 공유 시스템은 분산 환경에서 신뢰 수준을 구축하고 일관된 메시지를 기록 및 공유함으로써 서비스의 상호 운용성을 가능하게 한다. 그러나 허가형 블록체인은 종종 탈중앙화, 보안 및 상호 운용성과 충돌한다. 이는 중앙 집중식 시스템으로 돌아가거나 데이터의 독점 및 남용 및 오용으로 이어질 수 있다. 따라서 CAP (Consistency, Availability, Partition tolerance)에 이론 검증에 따라 메시지 공유, 비잔틴 내결함성 및 메시지 일관성을 고려하고 적용해야 한다. 기존의 PBFT(Practical Byzantine Fault Tolerance) 합의 알고리즘는 노드의 증가시, 장애내성을 갖기위해 계산되어야 할 합의 처리시간이 증가하며, DPOS(Delegated Proof of Stake) 알고리즘은 보상, 리더 선출의 공정성 문제 등에 따라 허가형 블록체인에서의 적합한 방식이 연구되고 있다. 본 논문에서는 서비스의 상호 운용성과 과제에 대해 논의하고 허가된 블록체인의 합의 개선을 통한 데이터 공유 시스템을 제안한다.

• Korean Journal of Microbiology
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• v.46 no.3
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• pp.240-247
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• 2010

Pseudomonas aeruginosa is a Gram (-) opportunistic human pathogen causing a wide variety of infections on lung, urinary tract, eyes, and burn wound sites and quorum sensing (QS), a cell density-sensing mechanism plays an essential role in Pseudomonas pathogenesis. In order to investigate the importance of QS in the Pseudomonas infections of Korean patients, we isolated 189 clinical strains of P. aeruginosa from the patients in Pusan Paik Hospital, Busan, South Korea. The QS signal production of these clinical isolates was measured by signal diffusion assay on solid media using reporter strains. While most clinical strains (79.4%) produced the QS signals as similar level as a wild type strain, PAO1 did, where LasR, the initial QS signal sensor-regulator was fully activated, a minority of them (4.2%) produced much less QS signals at the level to which LasR failed to respond. Similarly, while 72.5% of the clinical isolates produced QS signals enough to activate QscR, an another QS signal sensor-regulator, some few of them (9%) produced the QS signals at much lower level where QscR was not activated. For further analysis, we selected 74 clinical strains that were obtained from the patients under suspicion of Pseudomonas infection and investigated the total protease activity that is considered important for virulence. Interestingly, significant portion of them showed very low protease activity (44.6%) or no detectable protease activity (12.2%). When the biofilm-forming ability that is considered very important in chronic infection was examined, most isolates showed lower biofilm-forming activity than PAO1. Similarly, significant portion of clinical isolates showed reduced motility (reduced swarming activity in 51.4% and reduced twitching activity in 41.9%), or non-detectable motility (swarming-negative in 28.4% and twitching-negative in 28.4%). Our result showed that the clinical isolates that produced QS signals at the similar level to wild type could have significantly reduced activities in the protease production, biofilm formation, and motility, and some clinical isolates had unique patterns of motility, biofilm formation, and protease production that are not correlated to their QS activity.

• Microbiology and Biotechnology Letters
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• v.50 no.4
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• pp.548-556
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• 2022

Acylases can have a significant effect on biofilm formation owing to their quorum quenching activity. In this study, we investigated the effects of acylase treatment episodes on multispecies biofilm development. A consortium composed of 9 species belonging to different genera was allowed to form biofilms for 5 days under various treatment episodes (different treatment periods, 1, 2, 3, or 4 days; and two application timings, beginning or later) at 1, 5, 10, 20 and 50 mg·l^-1 acylase concentrations. The acylase treatment for 5 days showed that acylase concentration was negative with biofilm development (linear regression, Y = -0.05·x + 2.37, p < 0.05, R² = 0.88). Acylase was more effective in reducing biofilm formation when it was applied in the beginning (vs. in later development stage) at all acylase concentrations (p < 0.05). ANOVA indicated that treatment period was significant on biofilm formation in both application timings at ≥ 10 mg·l^-1 (p < 0.05). Linearity test results showed that all slope values between period and biofilm were negative in both timings at ≥ 10 mg·l^-1 (p < 0.05, except for the later application at 20 mg·l^-1). When temporal biofilm dynamics were monitored at 20 mg·l^-1, biofilms gradually increased with time at all treatment episodes (p < 0.05), and slope values in linear regression between biofilm and time were lower when acylase was applied in the beginning (p < 0.05). Our findings suggest the importance of the acylase treatment period and application timing on biofilm control.

• Journal of Microbiology and Biotechnology
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• v.23 no.5
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• pp.644-651
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• 2013

The fermented manure derivative known as Preparation 500 is traditionally used as a field spray in biodynamic agriculture for maintaining and increasing soil fertility. This work aimed at characterizing the product from a microbiological standpoint and at assaying its bioactive properties. The approach involved molecular taxonomical characterization of the culturable microbial community; ARISA fingerprints of the total bacteria and fungal communities; chemical elemental macronutrient analysis via a combustion analyzer; activity assays for six key enzymes; bioassays for bacterial quorum sensing and chitolipooligosaccharide production; and plant hormone-like activity. The material was found to harbor a bacterial community of $2.38{\times}10^8$ CFU/g dw dominated by Gram-positives with minor instances of Actinobacteria and Gammaproteobacteria. ARISA showed a coherence of bacterial assemblages in different preparation lots of the same year in spite of geographic origin. Enzymatic activities showed elevated values of ${\beta}$-glucosidase, alkaline phosphatase, chitinase, and esterase. The preparation had no quorum sensing-detectable signal, and no rhizobial nod gene-inducing properties, but displayed a strong auxin-like effect on plants. Enzymatic analyses indicated a bioactive potential in the fertility and nutrient cycling contexts. The IAA activity and microbial degradation products qualify for a possible activity as soil biostimulants. Quantitative details and possible modes of action are discussed.

• Journal of Life Science
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• v.27 no.10
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• pp.1161-1167
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• 2017

In order to find a new antimicrobial bacterium, we performed screening for antimicrobial activity of bacteria isolated from the eggs of a sea hare. The newly identified strain was designated as Phaeobacter inhibens KJ-2, based on the biochemical characterization and 16S rRNA gene sequence analysis. A colony of P. inhibens KJ-2 showed a circular and ruler-like smooth form at the edge, and a brown color. However, when maintained with a longer incubation time, its coloring was transformed into dark brown. From the result of SEM, P. inhibens KJ-2 is a bacillus which has a length of $0.8{\sim}1.0{\mu}m$ and a width of $0.4{\sim}0.6{\mu}m$. The optimal growth and antimicrobial activity were observed by shaking the culture for 24 hr at $20^{\circ}C$, which showed potent activity against pathogenic bacteria including Vibrio logei, Vibrio campbellii, Vibrio mimicus, Vibrio vulnificus, and Vibrio salmonicida. The antimicrobial activity was proportional to the amount of produced acylated homoserine lactones (AHLs). Therefore, we suggest that production of antimicrobial materials from P. inhibens KJ-2 is regulated by Quorum sensing (QS).