• Archives of Pharmacal Research
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• 제11권2호
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• pp.166-168
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• 1988

The effects of aglycones moring and quercetin and their corresponding glycosides quercitrin and rutin were studied on the blood glucose levels of rats. Quercetin and quercetrin caused hypoglycaemia in rats while rutin and morin showed almost no difference. Quercetin, which showed 50% pronounced hypoglycaemic effects, reduced significantly the blood glucose level of alloxan diabetic rats.

• Archives of Pharmacal Research
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• 제13권2호
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• pp.211-213
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• 1990

To test flavonoids for antibacterial activity against oral micraorganisms, flavonoids, quercetrin and naringenin, were incorporated into two pharmaceutical preparations in the form of tooth paste. Samplees of dental plaque, the msot accused dental deposit which initiates the gingival and periodental diseases, were collected from the teeth surface of ten dental students at one week interval before and after using placebo, followed by two formulae of tooth paste containing 0.1% of quercetrin and naringenin (formulas I and II, respectively). The amount of dental plaque was assessed by the quigley and Hens index. Then plaque samples were subjected to bacteriological examination of Gram stain and plate counts of microorganisms. The amount of dental plaque was assessed by the Quigley and Hens index. Then plaque samples were subjected to bacteriological examination of Gram stain and plate counts of microorganisms. The results revealed that most of Gram negative cocci and bacilli were highly affected by the two formulae : the number of actinomycetes were decreased after using formula I and disappeared completely by the sue of formula II, while the number of Gram positive streptococci was highly decreased after the treatment with the two formulae. These results indicate a possible use of flavonoids to inhibit dental plaque formation.

• Natural Product Sciences
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• 제4권1호
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• pp.9-14
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• 1998

Jaceidin, Jaceidin-7-O-methylether and quercetin were isolated from-Juniperus phoenicea L. alcoholic extract, however, Sequoiaflavone was isolated from Cupressus semperiverns L. In addition, the alcoholic extracts of both plants were found to contain also kaempferol-3-O-rhamnoside, quercetrin, myricitrin, cupressuflavone. The chemical identities of the isolated compounds were established using UV, IR, $^1H-and\;^{13}C-NMR$ spectroscopy.

• Natural Product Sciences
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• 제8권1호
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• pp.16-17
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• 2002

The flavonoids kaempferol, quercetin, myrecetin, kaempferol-3-O-rhamnoside and quercetrin has been isolated from the leaves of C. tamala and their structures were established by spectral analysis and direct comparison with authentic samples. This is the first report of occurrence of these compounds from C. tamala.

• Natural Product Sciences
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• 제24권4호
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• pp.272-283
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• 2018

The root-knot nematode, Meloidogyne incognita caused a serious damage to many plants. The phenolic components of the leaves of Schinus terebinthifolius were investigated as potential nematicidal agents for M. incognita. Nine compounds were isolated and characterized as viz., 1,2,3,4,6-pentagalloyl glucose (1), kaempferol-3-O-${\alpha}$-L-rhamnoside (Afzelin) (2), quercetin-3-O-${\alpha}$-L-rhamnoside (Quercetrin) (3), myricetin (4), myricetin-3-O-${\alpha}$-L-rhamnoside (Myricetrin) (5), methylgallate (6), protocatechuic acid (7), quercetin (8), and gallic acid (9) using nuclear magnetic resonance (NMR) spectroscopy. Compound 1 showed pronounced nematicidal activity compared to Oxamyl as a positive control. It showed the lowest eggs-hatchability (34%) and the highest mortality in nematode population (21% after 72 hours of treatment) at a concentration of $200{\mu}g/mL$. It exhibited the best suppressed total nematode population, root galling and number of eggmasses in infected tomato plants. The total carbohydrates and proteins were also significantly induced by 1 with reduction in total phenolics and increase in defense-related proteins. Thus, compound 1 could be a promising, more safe and effective natural nematicidal agent for the control of root-knot nematodes.

• Applied Biological Chemistry
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• 제48권1호
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• pp.89-92
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• 2005

삼백초 지상부의 추출조건을 설정하고자 추출용매 및 시간에 따른 이화학적 특성을 살펴보았다. 추출용매 조건은 40% 에탄올을 사용하여 추출할 때로 나타났으며, 이때, 총수율, 전자공여능, 총페놀함량, 총플라보노이드 함량은 각각 13.50%, 83.50%, 2.60 mg/ml 및 2.09 mg/ml으로 나타났다. 최적의 추출 시간은 4시간으로 나타났으며, 이때, 총수율, 전자공여능,총폐놀함량, 총플라보노이드 함량은 각각 12.79%, 71.13%, 2.83 mg/ml 및 2.16 mg/ml으로 나타났다. 삼백초 지상부를 40% 에탄을 추출물로 4시간 추출하였을때, quercetin 함량은 360.13 mg/100 g으로 나타났으며, quercetin 함량은 1379.54 mg/100 g으로 나타났다. 삼백초 지상부를 이용하여 추출조건을 설정한 결과 40% 에탄올을 이용하여 4시간 추출하는 것이 가장 적합한 것으로 나타났다.

• Advances in Traditional Medicine
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• 제6권4호
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• pp.355-360
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• 2006

Plants have been used in traditional medicinal system for centuries. Bangladeshi medicinal plants have received considerable attention from the researchers for evaluation of their bioactivity. As a part of our ongoing research of screening the Bangladeshi medicinal plants, the ethanolic extract of Dendrophthoe falcata have been chosen for the present study. The ethanolic extract of the leaves of the plant have been assessed for their antioxidant, antinociceptive, and general toxicity. The extract showed potent antioxidant activity ($IC_{50}5.1{\mu}g/ml$) using DPPH radical scavenging assay, which is comparable to the standard ascorbic acid ($IC_{50}4.6{\mu}g/ml$). The extract significantly and dose dependently inhibited the acetic acid induced writhing in mice (71.2%, P < 0.001 and 28.0%, P < 0.05 for 500 and 250 mg/kg body weight, respectively). A general toxicity was assessed by a simple and low cost assay using brine shrimp lethality as an indicator. The extract showed low level of toxicity ($LC_{50}100{\mu}g/ml$). Using different chromatographic techniques, quercitrin (quercetin 3-O-${\alpha}$-rhamnoside) was separated as the major component from the extract. The structure was elucidated by detailed 1D and 2D NMR and mass spectral analysis.

• 한국식품조리과학회지
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• 제11권3호
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• pp.303-308
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• 1995

참죽나무(Cedrela sinensis A.Juss) 잎을 재료로 하여, 김치, 나물, 부각 등의 조리하였을 때 이 식물의 flavonoid 화합물인 quercitrin의 함량 변화를 HPLC에 의한 용매계 THF-dioxane-MeOH-HOAc-5% H/Sub 3/PO/Sub 4/H/Sub 2/O(145:125:50:20:2:658)에서 분석하였다. 참죽김치를 5$^{\circ}C$에서 저장하였을 때의 quercitrin 함량은 1일째 13.06%, 6일째는 10.99%, 10일째는 11.49%, 20일째는 7.21%이며, 김치를 2$0^{\circ}C$에 저장하였을 시는 저장 1일째 15.33%, 6일째 10.83%, 10일째 9.85%, 20일째 5.78% 함유하고 있다. 나물의 경우 주성분인 quercitrin의 함량은 13.06%, 부각은 5.03%이었다. 따라서 김치의 경우 저장 일수가 길어짐에 따라 quercitrin함량은 감소하는 경향을 나타내며, 나물의 경우에는 quercetrin함량의 큰 변화를 볼 수 없고, 부각으로 조리한 경우 quercitrin의 함량은 급격히 감소하였다.

• 한국식품과학회지
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• 제31권3호
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• pp.815-822
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• 1999

우리 나라 전역에 자생하는 쑥(Artemisia vulgaris)의 전초의 수용성 에탄올추출물로 부터 21가지의 flavonoids를 분리하였다. 이들 화합물의 동정은 H-NMR, mass, UV-스펙트럼을 이용하여 동정하였으며, 동정된 flavonoids들은 tricin, jaceosidine, eupafolin, diosmetin, chrysoeriol, humoeridictyol, isorhamnetin, apigenin, eriodictyol, luteolin, luteolin 7-glucoside, kaempferol 3-glucoside, kaempferol 7-glucoside, kaempferol 3-rhamnoside, kaempferol 3-rutinoside, quercetin, quercetin 3-glucoside, quercetin 3-galactoside, quercetin, quercetin 7-glucoside, rutin 그리고 vitexin으로 동정 되었다. 이들 분리된 각 flavonoids들에 대하여 쥐의 간에서 추출한 마이크로좀에 대하여 지질과산화 효과를 살펴보았다. 이들 flavonoids화합물들의 항산화효과는 비타민 E와 비교하였을 때 높은 활성이 나타났다. 이미 강력한 항산화물질로 잘 알려진 quercetin, apigenin, eriodictyol등의 화합물의 $IC_{50}$ 값은 각각 0.9, 0.3, $0.3\;{\mu}g/mL$로 나타났으며, methoxylated flavonoids인 eupafolin, jaceosidine, diosmetin 등의 화합물도 $IC_{50}$ 값이 1.0, 1.4, $1.0\;{\mu}g/mL$로 나타나 비타민 E에 비교할 때 높은 활성을 나타냈다.

• 한국응용과학기술학회지
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• 제31권4호
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• pp.771-780
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• 2014

니아울리 잎 추출물의 항산화 효능과 성분 분석에 관한 연구를 시행하였다. 실험에 사용한 시료는 건조된 니아울리 잎의 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획을 이용하였다. 자유라디칼(1,1-phenyl-2-picrylhydrazyl, DPPH) 소거활성($FSC_{50}$)은 에틸아세테이트 분획이 $10.05{\mu}g/mL$로, 지용성 항산화제로 알려진 (+)-${\alpha}$-tocopherol ($8.89{\mu}g/mL$)과 유사한 효과를 나타내었다. 루미놀 발광법을 이용한 $Fe^{3+}-EDTA/H_2O_2$ 계에서 생성된 활성산소종(reactive oxygen species, ROS)에 대한 니아울리 잎 추출물의 총 항산화능($OSC_{50}$)에서 에틸아세테이트 분획은 $1.61{\mu}g/mL$, 아글리콘 분획은 $1.07{\mu}g/mL$를 나타냈다. 에틸아세테이트 분획과 아글리콘 분획 모두 항산화 활성이 매우 큰 비교 물질인 L-ascorbic acid ($1.50{\mu}g/mL$)와 비슷한 활성산소 소거활성을 나타내었다. Rose-bengal로 광증감된 $^1O_2$에 의한 세포손상에 대한 세포보호능 측정에서 추출물 및 분획 모두 농도 의존적($5{\sim}50{\mu}g/mL$)으로 세포보호 활성을 나타내었다. 특히 아글리콘 분획과 50% 에탄올 추출물의 ${\tau}_{50}$은 $10{\mu}g/mL$에서 각각 158.80 min, 50.1 min으로 지용성 항산화제로 알려진 (+)-${\alpha}$-tocopherol (38.0 min)보다 더 큰 세포 보호 효과를 나타내었다. TLC와 HPLC를 이용하여 니아울리 잎 추출물의 에틸아세테이트 분획으로부터 avicularin과 quercitrin이 존재함을 처음 확인하였다. 이상의 결과들은 니아울리 잎 추출물 또는 분획이 항산화 원료로서 화장품에 응용 가능함을 시사한다.