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http://dx.doi.org/10.12925/jkocs.2014.31.4.771

Antioxidative Effect and Component Analysis of Niaouli (Melaleuca quinquenervia) Leaf Extracts  

Kim, Moon Jin (Department of Fine Chemistry, Nanobiocosmetic Lab, Cosmetic R & D center, Seoul National University of Science and Technology)
Kim, Eun Jong (Department of Fine Chemistry, Nanobiocosmetic Lab, Cosmetic R & D center, Seoul National University of Science and Technology)
Park, Soo Nam (Department of Fine Chemistry, Nanobiocosmetic Lab, Cosmetic R & D center, Seoul National University of Science and Technology)
Publication Information
Journal of the Korean Applied Science and Technology / v.31, no.4, 2014 , pp. 771-780 More about this Journal
Abstract
The antioxidative effects and component analysis of the Melaleuca quinquenervia leaf extracts were investigated. All experiments were performed with 50% ethanol extract, ethyl acetate fraction and aglycone fraction obtained from dried M. quinquenervia leaves. The DPPH (1,1-phenyl-2-picrylhydrazyl) scavenging activity ($FSC_{50}$) of ethyl acetate fraction ($10.05{\mu}g/mL$) of M. quinquenervia leaf extracts was similar to (+)-${\alpha}$-tocopherol($8.89{\mu}g/mL$) known as a typical antioxidant. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of the ethyl acetate fraction ($1.61{\mu}g/mL$) and aglycone fraction ($1.07{\mu}g/mL$) of leaf extracts of M. quinquenervia on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system using the luminol-dependent chemiluminescence assay were similar to that of L-ascorbic acid ($1.50{\mu}g/mL$). The cellular protective effect of the extracts on the rose bengal sensitized photohemolysis of human erythrocytes was increased in a concentration dependant manner ($1{\sim}50{\mu}g/mL$). Especially, the cellular protective effects of Aglycone fraction (${\tau}_{50}=158.80min$) and 50% Ethanol extract (${\tau}_{50}=50.1{\pm}0.2min$) on the $^1O_2$-induced cellular damage of human cells were exhibited the higher than (+)-${\alpha}$-tocopherol (${\tau}_{50}=38.0min$). TLC and HPLC were used to analyse active components in the ethylacetate fraction of the extracts. Results showed that avicularin and quercetrin were active components of the extracts. These findings suggest that the M. quinquenervia leaf extracts can be applied to new cosmetics products as an effective antioxidant ingradient.
Keywords
Niaouli leaf extract; flavonoid; antioxidative activity; avicularin; quercitrin;
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