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Identification and Characterization of Pseudocercospora pyricola Causing Leaf Spots on Aronia melanocarpa

  • Park, Sung-Hee;Choi, In-Young;Seo, Kyoung-Won;Kim, Jin-Ho;Galea, Victor;Shin, Hyeon-Dong
    • Mycobiology
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    • v.45 no.1
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    • pp.39-43
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    • 2017
  • Leaf spot disease on black chokeberry (Aronia melanocarpa) was observed at several locations in Korea during 2014-2015. Leaf spots were distinct, scattered over the leaf surface and along the leaf border, subcircular to irregular and brown surrounded by a distinct dark color, and were expanded and coalesced into irregularly shaped lesions. Severely infected leaves became dry and fell off eventually. The causative agent was identified as Pseudocercospora pyricola. Morphological observations and phylogenetic analyses of multiple genes, including internal transcribed spacer, translation elongation factor 1-alpha, actin, and the large subunit ribosomal DNA were conducted. The pathogenicity test was conducted twice yielding similar results, fulfilling Koch's postulates. To our knowledge, this is the first report on P. pyricola infection of A. melanocarpa globally.

Study of physical simulation of electrochemical modification of clayey rock

  • Chai, Zhaoyun;Zhang, Yatiao;Scheuermann, Alexander
    • Geomechanics and Engineering
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    • v.11 no.2
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    • pp.197-209
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    • 2016
  • Clayey rock has large clay mineral content. When in contact with water, this expands considerably and may present a significant hazard to the stability of the rock in geotechnical engineering applications. This is particularly important in the present work, which focused on mitigating some unwelcomed properties of clayey rock. Changes in its physical properties were simulated by subjecting the rock to a low voltage direct current (DC) using copper, steel and aluminum electrodes. The modified mechanism of the coupled electrical and chemical fields acting on the clayey rock was analyzed. It was concluded that the essence of clayey rock electrochemical modification is the electrokinetic effect of the DC field, together with the coupled hydraulic and electrical potential gradients in fine-grained clayey rock, including ion migration, electrophoresis and electro-osmosis. The aluminum cathodes were corroded and generated gibbsite at the anode; the steel and copper cathodes showed no obvious change. The electrical resistivity and uniaxial compressive strength (UCS) of the modified specimens from the anode, intermediate and cathode zones tended to decrease. Samples taken from these zones showed a positive correlation between electric resistivity and UCS.

Erythrolobus australicus sp. nov. (Porphyridiophyceae, Rhodophyta): a description based on several approaches

  • Yang, Eun-Chan;Scot, Joe;West, John A.;Yoon, Hwan-Su;Yokoyama, Akiko;Karsten, Ulf;De Goer, Susan Loiseaux;Orlova, Evguenia
    • ALGAE
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    • v.26 no.2
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    • pp.167-180
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    • 2011
  • The unicellular marine red alga Erythrolobus australicus sp. nov. (Porphyridiophyceae) was isolated into laboratory culture from mangroves in Queensland and New South Wales, Australia. The single multi-lobed red to rose-red plastid has more than one pyrenoid and lacks a peripheral thylakoid. Arrays of small electron dense globules occur along the thylakoids. The nucleus is peripheral with a central to eccentric nucleolus. Each Golgi body is associated with a mitochondrion. The spherical cells are positively phototactic with slow gliding movement. The psaA + psbA phylogeny clearly showed that E. australicus is a distinct species, which is closely related to E. coxiae. The chemotaxonomically relevant and most abundant low molecular weight carbohydrate in E. australicus is floridoside with concentrations between 209 and 231 ${\mu}mol g^{-1}$ dry weight. Traces of digeneaside were also detected. These various approaches help to understand the taxonomic diversity of unicellular red algae.

Identification and Characterization of Cercospora malayensis Causing Leaf Spot on Kenaf

  • Park, Sung-Hee;Choi, In-Young;Lee, Wang-Hyu;Lee, Kui-Jae;Galea, Victor;Shin, Hyeon-Dong
    • Mycobiology
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    • v.45 no.2
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    • pp.114-118
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    • 2017
  • In September 2013 and 2014, a significant number of kenaf plants showing symptoms of leaf spots with approximately 50% incidence were found in experimental plots in Iksan and Namwon, Korea. Leaf spots were circular to irregular, more or less vein-limited, reaching to 10 mm in diameter. The spots were initially uniformly brown to reddish brown, turning pale brown with a purplish margin and showing grayish patches on the lesion due to heavy fructification. The causative agent of the leaf spot disease was identified as Cercospora malayensis. The pathogenicity test was conducted with similar results, which fulfilled Koch's postulates. This is the first report of C. malayensis infection of kenaf in Korea.

Condition Classification for Small Reciprocating Compressors Using Wavelet Transform and Artificial Neural Network (웨이브릿 변환과 인공신경망 기법을 이용한 소형 왕복동 압축기의 상태 분류)

  • Lim, D.S.;Yang, B.S.;An, B.H.;Tan, A.;Kim, D.J.
    • Journal of Power System Engineering
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    • v.7 no.2
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    • pp.29-35
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    • 2003
  • The monitoring and diagnostics of the rotating machinery have been received considerable attention for many years. The objectives are to classify the machinery condition and to find out the cause of abnormal condition. This paper describes a classification method of diagnosing the small reciprocating compressor for refrigerators using the artificial neural network and the wavelet transform. In order to extract salient features, the wavelet transform are used from primary noise signals. Since the wavelet transform decomposes raw time-waveform signals into two respective parts in the time space and frequency domain, more and better features can be obtained easier than time-waveform analysis. In the training phase for classification, self-organizing feature map(SOFM) and learning vector quantization(LVQ) are applied, and the accuracies of them ate compared with each other. This paper is focused on the development of an advanced signal classifier to automatize the vibration signal pattern recognition. This method is verified by small reciprocating compressors, for refrigerator and normal and abnormal conditions are classified with high flexibility and reliability.

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Behavior of circular concrete-filled steel tubular columns under pure torsion

  • Ding, Fa-xing;Fu, Qiang;Wen, Bing;Zhou, Qi-shi;Liu, Xue-mei
    • Steel and Composite Structures
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    • v.26 no.4
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    • pp.501-511
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    • 2018
  • Concrete-filled steel tubular (CFT) columns are commonly used in engineering structures and always subjected to torsion in practice. This paper is thus devoted to investigate the mechanical behavior of circular CFT columns under pure torsion.3D finite element models based on reasonable material constitutive relation were established for analyzing the load-strain ($T-{\gamma}$) curves of circular CFT columns under pure torsion. The numerical simulation indicated that local bulking of the steel tube in CFT columns was prevented and the shear strength and ductility of the core concrete were significantly improved due to the confinement effect between the steel tube and the core concrete. Based on the results, formulas to predict the torsional ultimate bearing capacity of circular CFT columns were proposed with satisfactory correspondence with experimental results. Besides, formulas of composite shear stiffness and the overall process of the $T-{\gamma}$ relation of circular CFT columns under pure torsion were proposed.

Behavior of polygonal concrete-filled steel tubular stub columns under axial loading

  • Zhang, Tao;Ding, Fa-xing;Wang, Liping;Liu, Xue-mei;Jiang, Guo-shuai
    • Steel and Composite Structures
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    • v.28 no.5
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    • pp.573-588
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    • 2018
  • The objective of this paper is to investigate the mechanical performances of polygonal concrete-filled circular steel tubular (CFT) stub columns under axial loading through combined experimental and numerical study. A total of 32 specimens were designed to investigate the effect of the concrete strength and steel ratio on the compressive behavior of polygonal CFT stub columns. The ultimate bearing capacity, ductility and confinement effect were analyzed based on the experimental results and the failure modes were discussed in detail. Besides, ABAQUS was adopted to establish the three dimensional FE model. The composite action between the core concrete and steel tube was further discussed and clarified. It was found that the behavior of CFT stub column changes with the change of the cross-section, and the change is continuous. Finally, based on both experimental and numerical results, a unified formula was developed to estimate the ultimate bearing capacity of polygonal CFT stub columns according to the superposition principle with rational simplification. The predicted results showed satisfactory agreement with both experimental and FE results.

Estimation of In vitro Digestibility of Barley Straw by Using a Homogenized Rumen Fluid and Artificial Saliva Mixed with Nitrogen and Energy Sources

  • Chaudhry, Abdul S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.11 no.1
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    • pp.13-16
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    • 1998
  • A $2{\times}2{\times}4$ factorial study was conducted to examine the possibility of improving estimates of in vitro digestibility, using untreated (UBS) and ammonia-treated (ABS) barley straw, through homogenization of rumen fluid (RF) and by additions of urea (U) and casein (C) as N sources and Xylose + Glucose (XG) as energy sources into artificial saliva. Digestibiltiy of ABS was significantly greater than that of UBS (p < 0.001). There was a significant decrease in digestibility when additions (U, UC, UCXG) were compared with the control (p < 0.001). A 2-way interaction between RF and straw type was significant (p < 0.05) for dry matter digestibility (DMD). Homogenization of RF increased DMD of ABS (p < 0.05) whereas it decreased DMD of UBS (p > 0.05). The study showed that addition of N alone or in combination with energy sources was not better than control, rather the reverse, digestion was inhibited by a combination of U and casein (UC). It was concluded that sufficient N and branched chain fatty acids were supplied in the inoculum from sheep fed high protein grass cubes to support the growth of cellulolytic microbes during in vitro incubation. Further studies are, however, required to explore the potential of homogenization in improving the in vitro method to estimate digestibility of cereal straws.

An Orbital Stability Study of the Proposed Companions of SW Lyncis

  • Hinse, T.C.;Horner, Jonathan;Wittenmyer, Robert A.
    • Journal of Astronomy and Space Sciences
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    • v.31 no.3
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    • pp.187-197
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    • 2014
  • We have investigated the dynamical stability of the proposed companions orbiting the Algol type short-period eclipsing binary SW Lyncis (Kim et al. 2010). The two candidate companions are of stellar to substellar nature, and were inferred from timing measurements of the system's primary and secondary eclipses. We applied well-tested numerical techniques to accurately integrate the orbits of the two companions and to test for chaotic dynamical behavior. We carried out the stability analysis within a systematic parameter survey varying both the geometries and orientation of the orbits of the companions, as well as their masses. In all our numerical integrations we found that the proposed SW Lyn multi-body system is highly unstable on time-scales on the order of 1000 years. Our results cast doubt on the interpretation that the timing variations are caused by two companions. This work demonstrates that a straightforward dynamical analysis can help to test whether a best-fit companion-based model is a physically viable explanation for measured eclipse timing variations. We conclude that dynamical considerations reveal that the proposed SW Lyncis multi-body system most likely does not exist or the companions have significantly different orbital properties from those conjectured in Kim et al. (2010).

Transferability of Cupped Oyster EST (Expressed Sequence Tag)-Derived SNP (Single Nucleotide Polymorphism) Markers to Related Crassostrea and Ostrea Species

  • Kim, Woo-Jin;Jung, Hyungtaek;Shin, Eun-Ha;Baek, Ilseon
    • The Korean Journal of Malacology
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    • v.30 no.3
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    • pp.197-210
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    • 2014
  • Single nucleotide polymorphisms (SNPs) are widely acknowledged as the marker of choice for many genetic and genomic applications because they show co-dominant inheritance, are highly abundant across genomes and are suitable for high-throughput genotyping. Here we evaluated the applicability of SNP markers developed from Crassostrea gigas and C. virginica expressed sequence tags (ESTs) in closely related Crassostrea and Ostrea species. A total of 213 putative interspecific level SNPs were identified from re-sequencing data in six amplicons, yielding on average of one interspecific level SNP per seven bp. High polymorphism levels were observed and the high success rate of transferability show that genic EST-derived SNP markers provide an efficient method for rapid marker development and SNP discovery in closely related oyster species. The six EST-SNP markers identified here will provide useful molecular tools for addressing questions in molecular ecology and evolution studies including for stock analysis (pedigree monitoring) in related oyster taxa.