Browse > Article
http://dx.doi.org/10.5941/MYCO.2017.45.1.39

Identification and Characterization of Pseudocercospora pyricola Causing Leaf Spots on Aronia melanocarpa  

Park, Sung-Hee (Department of Physical Medicine and Rehabilitation, Chonbuk National University Medical School)
Choi, In-Young (Jeollabuk-do Agricultural Research and Extension Services)
Seo, Kyoung-Won (Jeollabuk-do Agricultural Research and Extension Services)
Kim, Jin-Ho (Jeollabuk-do Agricultural Research and Extension Services)
Galea, Victor (School of Agriculture and Food Sciences, The University of Queensland)
Shin, Hyeon-Dong (Division of Environmental Science and Ecological Engineering, Korea University)
Publication Information
Mycobiology / v.45, no.1, 2017 , pp. 39-43 More about this Journal
Abstract
Leaf spot disease on black chokeberry (Aronia melanocarpa) was observed at several locations in Korea during 2014-2015. Leaf spots were distinct, scattered over the leaf surface and along the leaf border, subcircular to irregular and brown surrounded by a distinct dark color, and were expanded and coalesced into irregularly shaped lesions. Severely infected leaves became dry and fell off eventually. The causative agent was identified as Pseudocercospora pyricola. Morphological observations and phylogenetic analyses of multiple genes, including internal transcribed spacer, translation elongation factor 1-alpha, actin, and the large subunit ribosomal DNA were conducted. The pathogenicity test was conducted twice yielding similar results, fulfilling Koch's postulates. To our knowledge, this is the first report on P. pyricola infection of A. melanocarpa globally.
Keywords
Aronia melanocarpa; Multigene phylogenetic analysis; Mycosphaerella; Pseudocercospora leaf spot; Pseudocercospora pyricola;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Crous PW, Braun U, Hunter GC, Wingfield MJ, Verkley GJ, Shin HD, Nakashima C, Groenewald JZ. Phylogenetic lineages in Pseudocercospora. Stud Mycol 2013;75:37-114.   DOI
2 Hsieh WH, Goh TK. Cercospora and similar fungi from Taiwan. Taiwan: Maw Chang Book Company; 1990.
3 Nakashima C, Kobayashi T. Addition and reexamination of Japanese species belonging to the genus Cercospora and allied genera III. Species described by Japanese mycologists (2). Mycoscience 2000;41:25-31.   DOI
4 Carbone I, Kohn LM. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 1999;91:553-6.   DOI
5 O’Donnell K, Kistler HC, Cigelnik E, Ploetz RC. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proc Natl Acad Sci U S A 1998;95:2044-9.   DOI
6 White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. San Diego (NY): Academic Press; 1990. p. 315-22.
7 Crous PW, Schoch CL, Hyde KD, Wood AR, Gueidan C, De Hoog GS, Groenewald JZ. Phylogenetic lineages in the Capnodiales. Stud Mycol 2009;64:17-47.   DOI
8 Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 1990;172:4238-46.   DOI
9 Hunter GC, Wingfield BD, Crous PW, Wingfield MJ. A multi-gene phylogeny for species of Mycosphaerella occurring on Eucalyptus leaves. Stud Mycol 2006;55:147-61.   DOI
10 Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725-9.   DOI
11 Wee JI, Park JH, Baek CG, You YH, Chang T. First report of leaf spot caused by Alternaria tenuissima on black chokeberry (Aronia melanocarpa) in Korea. Mycobiology 2016;44:187-90.   DOI
12 Kulling SE, Rawel HM. Chokeberry (Aronia melanocarpa): a review on the characteristic components and potential health effects. Planta Med 2008;74:1625-34.   DOI
13 Hwang ES, Lee YJ. Quality characteristics and antioxidant activities of yanggaeng with aronia juice. J Korean Soc Food Sci Nutr 2013;42:1220-6.   DOI
14 Farr DF, Rossman AY. Fungal Databases, U.S. National Fungus Collection, ARS, USDA [Internet]. Washington, DC: United States Department of Agriculture, Agricultural Research Service; 2016 [cited 2016 Sep 27]. Available from: http://nt.ars-grin. gov/fungaldatabases/.
15 Kwon JH, Kang DW, Lee SY, Choi O, Kim J. First report of brown leaf spot caused by Alternaria alternata on Aronia melanocarpa in Korea. Plant Dis 2016;100:1011.
16 Hahm SS, Kwon MK, Kim BR, Han KS, Nam YG. Alternaria leaf spot caused by Alternaria mali on black chokeberry in Korea. Res Plant Dis 2016;22:50-4.   DOI
17 Chupp C. A monograph of the fungus genus Cercospora. Ithaca (NY): Author; 1954.
18 Crous PW, Aptroot A, Kang JC, Braun U, Wingfield MJ. The genus Mycosphaerella and its anamorphs. Stud Mycol 2000; 45:107-21.
19 Tang AM, Jeewon R, Hyde KD. A re-evaluation of the evolutionary relationships within the Xylariaceae based on ribosomal and protein-coding gene sequences. Fungal Divers 2009;34:127-55.
20 Stewart EL, Liu Z, Crous PW, Szabo LJ. Phylogenetic relationships among some cercosporoid anamorphs of Mycosphaerella based on rDNA sequence analysis. Mycol Res 1999;103:1491-9.   DOI
21 Crous PW, Kang JC, Braun U. A phylogenetic redefinition of anamorph genera in Mycosphaerella based on ITS rDNA sequence and morphology. Mycologia 2001;93:1081-101.   DOI
22 Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W; Fungal Barcoding Consortium; Fungal Barcoding Consortium Author List. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc Natl Acad Sci U S A 2012;109:6241-6.   DOI