• Journal of Applied Biological Chemistry
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• 제44권4호
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• pp.163-166
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• 2001

Piezoelectric (PZ) crystal biosensor system was used to detect the DNA of food pathogenic Listeria monocytogenes. L. monocytogenes-specific DNA was multiplied via the polymerase chain reaction using LM1 oligonucleotide (5'-TTACGAATTAAAAAGGAGCG-3') and LM2 oligonucleotide (5'-TTAAATCAGCAGGGGTCTTT-3') as primers. DNA fragment of 161 bp, which was specific only for L. monocytogenes, was observed. To obtain a large amount of single-stranded DNA containing an SH group used for coupling to the gold electrode chemisorptively, LM1 oligonucleotide containing a mercaptohexyl group was utilized as a single strand PCR primer. The PCR product was immobilized onto the gold electrode of PZ crystal, and hybridization was monitored in quartz crystal microbalance (QCM) system by injecting the antisense single-stranded DNA of 161 nucleotides obtained via the single strand PCR using the unmodified LM2 primer. Approximately 70 Hz of frequency drop was observed in the QCM system in the case of two consecutive injections of $5{\mu}g$ of the antisense single-stranded DNA.

• 한국항만학회지
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• 제14권3호
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• pp.373-378
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• 2000

Oil spill in sea water is the most frequent and significant problem of marine pollution. As an early detection sensor of the pollution, an activated carbon coated quartz crystal is prepared and examined for its performance of detection sensitivity and stability. Powdered activated carbon and phenol resin is coated on the surface of the sensor and the sensor is baked for an hour. Adsorption of acetone dissolved in water and salt water is measured using frequency shift of quartz crystal at different concentrations of solute material. The outcome indicates that the sensor preparation is adequate and the measurement of solute concentration is stable and sensitive enough to be implemented on the monitoring of solute concentration is stable and sensitive enough to be implemented in the monitoring of organic pollution of sea water.

• Bulletin of the Korean Chemical Society
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• 제25권7호
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• pp.1031-1035
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• 2004

The adsorption kinetics of ${\beta}$-casein on a hydrophobic surface has been studied by means of the quartz crystal microbalance (QCM). The self assembled monolayer of 1-octadecanethiol on a gold coated quartz crystal was used as a hydrophobic surface for adsorption. The adsorption kinetics was monitored in different solution conditions. Formation of monolayer is observed in most cases. At high concentration of protein, micelle formation which is interrupted by high ionic strength of solution is observed. Casein binding cations such as $Ca^{2+},\;Ba^{2+}\;and\;Al^{3+}$ increase the hydrophobicity of the protein and the multiple layer adsorption occurs. The strong and weak points of the QCM method in the study of protein adsorption are discussed.

• 한국해양공학회지
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• 제17권6호
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• pp.77-82
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• 2003

The absorption of water into an anti-corrosive organic coating, such as alkyd and urethane resin coating, was investigated, using a quartz crystal microbalance (QCM). Anticorrosive properties of the coatings were also measured, by means of electrochemical impedance spectroscopy (EIS). The overall absorption of water in the coating is determined by the chemical nature of resin, and decreases with increasing thickness. The enhancement of anti-corrosive performance, through increase of coating thickness, was more remarkable in the case of the coating that hadlower equilibrium water absorption. The absorption kinetics curves were Fickian in nature. The EIS analysis confirmed that the resin, having lower equilibrium water absorption, shows higher anti-corrosive performan.

• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제48권6호
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• pp.442-450
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• 1999

In this paper, we have fabricated Langmuir-Blodgett(LB) films by LB technique and evaluated the deposited status of LB films by UV-vis absorbance. It was found thatthe thickness of LB films per a layer are $27~30[{\AA}]$ by ellipsometry. The responeses between LB films and organic gases were investigated using by I-V characteristics of LB films and F-R diagram of quartz crystal. The response orders between LB films and organic gases observed by I-V characteristics were as following ; chloroform, methanol, acetone and ethanol in the order of their short chain length. The response mechanism between LB films and organic gases observed by F-R diagram of quartz crystal could be modeled on adsorption at surface, penetration, desorption at surface and inside.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1993년도 정기총회 및 추계학술대회 논문집 학회본부
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• pp.246-248
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• 1993

We investigated the responses of six types of lipid-coated AT-cut quartz crystal resonators to chemical vapour sensors. The responses of quartz crystal at 9 MHz coated with phosphatidylglycerol (PG), phosphatidylinositol(PI), phosphatidylethanolamine(PE), phosphatidylserine(PS), and lipid A(LA) were obtained for amyl acetate, acetoin, menthane and other organic gases which showed different affinities for each lipid. The identification of odorants depending on the species of lipid used for coating is discussed in terms of the normalized resonant frequency shift pattern.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1992년도 하계학술대회 논문집 A
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• pp.498-500
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• 1992

In this paper, we described a evaluating technique of deposition status of LB films by quartz crystal. As a deposited material for this experiments, arachidic acid and stearic acid were used. We observed the deposition status of LB films on the surface of quartz crystal. The experimental results showed that Y type H films are deposited according to frequency shift, deposition ratio, and SEM photographs etc.

• 센서학회지
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• 제18권2호
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• pp.160-167
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• 2009

In this study, secondary antibody-containing quartz crystal microbalance(QCM) sensor chip was prepared and utilized for the detection of human and bovine haptoglobin. Anti-goat immunoglobulin G antibody, which is a secondary antibody capable of capturing primary antibodies raised in goat, was immobilized through the reaction between hydrazide and aldehyde group prepared on the QCM surface and antibody respectively. The resulting sensor chip showed higher stability in the repeated surface regeneration with acidic dissociation solution as well as requiring lower amount of primary antibody when compared to the protein G sensor chip. The secondary antibody sensor chip was applied for the estimation of bovine and human haptoglobin.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1997년도 하계학술대회 논문집 C
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• pp.1522-1524
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• 1997

In this paper, we measured the impedence |Z| and phase angle $\theta$ using the Impedence Analyzer (Hp 4192A). When frequency is varied to produce resonance, the curves(admittance diagram) are obtained. The resonant frequency is obtained also. We analyzed the operating characteristics of quartz crystal coated with sensitive LB films. That is the size of circle in the admittance diagram is decreased as to coat sensitive LB films on the quartz crystal. The resonant frequency and resistance is changed with coating on the surface.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1996년도 추계학술대회 논문집 학회본부
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• pp.261-263
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• 1996

The study on the development of one-channel gas sensor using the quartz crystal analyzer were attempted. The adsorption and desorption behavior of organic gases were investigated using the resonant frequency and resistance method of quartz crystal. The sensitive materials were deposited on the quartz crystal analyzer(QCA) by using Langmuir-Blodgett method. To investigate the response characteristics of organic vapours and response mechanism, resonant frequency-resonant resistance (F-R) diagram was used. In our experimental results, the response mechanism between sensitive LB film and organic vapours was obtained using F-R diagram. And the position of each organic vapour were different as to the kind and injection amount. Thus F-R diagram can be applied to one-channel gas sensor using the QCA and useful to analyze the response mechanism between organic vspours and sensitive LB films.