Browse > Article
http://dx.doi.org/10.5012/bkcs.2004.25.7.1031

QCM Study of β-Casein Adsorption on the Hydrophobic Surface: Effect of Ionic Strength and Cations  

Lee, Myung-Hee (Department of Chemistry, Keimyung University)
Park, Su-Kyung (Department of Chemistry, Keimyung University)
Chung, Chin-Kap (Department of Chemistry, Keimyung University)
Kim, Hack-Jin (Department of Chemistry, Chungnam National University)
Publication Information
Bulletin of the Korean Chemical Society / v.25, no.7, 2004 , pp. 1031-1035 More about this Journal
Abstract
The adsorption kinetics of ${\beta}$-casein on a hydrophobic surface has been studied by means of the quartz crystal microbalance (QCM). The self assembled monolayer of 1-octadecanethiol on a gold coated quartz crystal was used as a hydrophobic surface for adsorption. The adsorption kinetics was monitored in different solution conditions. Formation of monolayer is observed in most cases. At high concentration of protein, micelle formation which is interrupted by high ionic strength of solution is observed. Casein binding cations such as $Ca^{2+},\;Ba^{2+}\;and\;Al^{3+}$ increase the hydrophobicity of the protein and the multiple layer adsorption occurs. The strong and weak points of the QCM method in the study of protein adsorption are discussed.
Keywords
Adsorption; ${\beta}$-Casein; Quartz crystal microbalance (QCM); Ionic strength; Cation;
Citations & Related Records

Times Cited By Web Of Science : 3  (Related Records In Web of Science)
Times Cited By SCOPUS : 3
연도 인용수 순위
1 Clasesson, P. M.; Blomberg, E.; Froberg, J. C.; Nylander, T.; Arnebrant, T. Adv. Colloid Interface Sci. 1995, 57, 161.   DOI   ScienceOn
2 Leermakers, F. A. M.; Atkinson, P. J.; Dickinson, E.; Horne, D. S. J. Colloid Interface Sci. 1996, 178, 681.   DOI   ScienceOn
3 Dubin, S. B.; Lunac, J. H.; Benedek, G. B. Proc. Natl. Acad. Sci. USA 1967, 57, 1164.   DOI   ScienceOn
4 Yan, L.; Zhang, Y.; Cui, G.; Li, J. Colloids Surf. A 2000, 175, 61.   DOI   ScienceOn
5 Ha, T. H.; Kim, K. Langmuir 2001, 17, 1999.   DOI   ScienceOn
6 Rickert, J.; Brecht, A.; Gopel, W. Biosensors Bioelectronics 1997, 12, 567.   DOI   ScienceOn
7 Hemar, Y.; Horne, D. S. J. Colloid Interface Sci. 1998, 206, 138.   DOI   ScienceOn
8 Fragneto, G.; Thomas, R. K.; Rennie, A. R.; Penfold, J. Science 1995, 267, 657.   DOI   ScienceOn
9 Hook, F.; Rodahl, M.; Kasemo, B.; Brzenzinski, P. Proc. Natl. Acad. Sci. USA 1998, 95, 12271.   DOI
10 Murray, B. S.; Cros, L. Colloids Surf. B 1998, 10, 227.   DOI   ScienceOn
11 Roedahl, M.; Kasemo, G. J. Sens. Actuators A 1996, 54, 448.   DOI   ScienceOn
12 Kull, T.; Nylander, T.; Tiberg, F.; Wahlgren, N. M. Langmuir 1997, 13, 5141.   DOI   ScienceOn
13 Molina-Bolivar, J. O.; Ortega-Vinuesa, J. L. Langmuir 1999, 15, 2644.   DOI   ScienceOn
14 Dickinson, E.; Stainsby, G. Colloids in Food; Applied Science: London, 1982.
15 Zhang, Z.; Dalgleish, D. G.; Goff, H. D. Colloids Surf. B 2004, 34, 113.   DOI   ScienceOn
16 Russev, S. C.; Arguirov, T. V.; Gurkov, T. D. Colloids Surf. B 2000, 19, 89.   DOI   ScienceOn
17 Aptel, J. D.; Voegel, J. C.; Schmitt, A. Colloids and Surfaces 1988, 29, 359.   DOI   ScienceOn
18 Krisdhasima, V.; Vinaraphong, P.; McGuire, J. J. Colloid Interface Sci. 1993, 161, 325.   DOI   ScienceOn
19 Janshoff, A.; Galla, H.-J.; Steinem, C. Angew. Chem. Int. Ed. 2000, 39, 4004.   DOI   ScienceOn
20 Caessens, P. W. J. R.; De Jongh, H. H. J.; Norde, W.; Gruppen, H. Biochim. Biophys. Acta 1999, 1430, 73.   DOI   ScienceOn
21 Nylander, T.; Tiberg, F.; Su, T.-J.; Lu, J. R.; Thomas, R. K. Biomacromolecules 2001, 2, 278.   DOI   ScienceOn