Browse > Article
http://dx.doi.org/10.5369/JSST.2009.18.2.160

Detection of human and bovine haptoglobin by using quartz crystal microbalance sensor chip containing secondary antibody  

Kim, Sung-Il (AMO Co., Ltd.)
Ha, In-Young (Department of Chemistry, Kangnung National University)
Choi, Suk-Jung (Department of Chemistry, Kangnung National University)
Publication Information
Journal of Sensor Science and Technology / v.18, no.2, 2009 , pp. 160-167 More about this Journal
Abstract
In this study, secondary antibody-containing quartz crystal microbalance(QCM) sensor chip was prepared and utilized for the detection of human and bovine haptoglobin. Anti-goat immunoglobulin G antibody, which is a secondary antibody capable of capturing primary antibodies raised in goat, was immobilized through the reaction between hydrazide and aldehyde group prepared on the QCM surface and antibody respectively. The resulting sensor chip showed higher stability in the repeated surface regeneration with acidic dissociation solution as well as requiring lower amount of primary antibody when compared to the protein G sensor chip. The secondary antibody sensor chip was applied for the estimation of bovine and human haptoglobin.
Keywords
secondary antibody; immunosensor; quartz crystal microbalance(QCM); haptoglobin;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 M. Nisnevitch and M. A. Firer, 'The solid phase in affinity chromatography: strategies for antibody attachment', J. Biochem. Biophys. Methods, vol. 49, pp. 467-480, 2001   DOI   ScienceOn
2 E. Briand, M. Salmain, J. M. Herry, H. Perrot, C. Compere and C. M. Pradier, 'Building of an immunosensor: how can the composition and structure of the thiol attachment layer affect the immunosensor efficiency?', Biosens. Bioelectron., vol. 22, pp. 440-448, 2006   DOI   ScienceOn
3 M. J. O’Brien II, S. R. J. Brueck, V. H. Perez-Luna, L. M. Tender, and G. P. Lopez, 'SPR biosensors: simultaneously removing thermal and bulk composition effects', Biosens. Bioelectron., vol. 14, pp. 145-154, 1999   DOI   ScienceOn
4 M. Åkerstedt, L. Bjorck, K. P. Waller, and Å. Sternesjo, 'Biosensor assay for determination of haptoglobin in bovine milk', J. Dairy Res., vol. 73, pp. 299-305, 2006   DOI   ScienceOn
5 J. W. Chung, J. M. Park, R. Bernhardt, and J. C. Pyun, 'Immunosensor with a controlled orientation of antibodies by using NeutrAvidin-protein. A complex at immunoaffinity layer', J. Biotechnol., vol. 126, pp. 325-333, 2006   DOI   ScienceOn
6 하인영, 최석정, 'Protein G를 포함하는 수정미소저 울 센서 칩과 정제되지 않은 항혈청을 이용한 헵토 글로빈과 트랜스페린의 면역분석', 센서학회지, 제 17권, 제5호, pp. 380-386, 2008   DOI
7 김성일, 이현정, 박순혁, 방현수, 이진아, 최석정, '헵토글로빈 측정을 위한 수정미소저울 바이오센서 개발', 센서학회지, 제16권, 제2호, pp. 132-141, 2007   DOI
8 M. Kotaka, G. G. Chen, P. B. Lai, W. Y. Lau, P. K. Chan, T. W. Leung, and A. K. Li, 'Analysis of differentially expressed genes in hepatocellular carcinoma with hepatitis C virus by suppression subtractive hybridization', Oncol. Res., vol. 13, pp. 161-167, 2002   ScienceOn
9 Y. Kaneta, Y. Kagami, T. Tsunoda, R. Ohno, Y. Nakamura, and T. Katagiri, 'Genome-wide analysis of gene-expression profiles in chronic myeloid leukemia cells using a cDNA microarray', Int. J. Oncol., vol. 23, pp. 681-691, 2003   ScienceOn
10 B. Ye, D. W. Cramer, S. J. Skates, S. P. Gygi, V. Pratomo, L. Fu, N. K. Horick, L. J. Licklider, J. O. Schorge, R. S. Berkowitz, and S. C. Mok, 'Haptoglobin alpha subunit as potential serum biomarker in ovarian cancer: identification and characterization using proteomic profiling and mass spectrometry', Clin. Cancer. Res., vol. 9, pp. 2904-2911, 2003   ScienceOn
11 H. F. Juan, J. H. Chen, W. T. Hsu, S. C. Huang, S. T. Chen, J. Y.-C. Lin, Y. W. Chang, C. Y. Chiang, L. L. Wen, D. C. Chan, Y. C. Liu, and Y. J. Chen, 'Identification of tumor-associated plasma biomarkers using proteomic techniques: from mouse to human', Proteomics, vol. 4, pp. 2766-2775, 2004   DOI   ScienceOn
12 W. Lee, B. K. Oh, Y. M. Bae, S. H. Paek, W. H. Lee, and J. W. Choi, 'Fabrication of self-assembled protein A monolayer and its application as an immunosensor', Biosens. Bioelectron., vol. 19, pp. 185-192, 2003   DOI   ScienceOn
13 H. C. Lu, H. M. Chen, Y. S. Lin, and J. W. Lin, 'A reusable and specific protein A-coated piezoelectric biosensor for flow injection immunoassay', Biotechnol. Prog., vol. 16, pp. 116-124, 2000   DOI   ScienceOn