• Journal of Animal Science and Technology
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• 제48권2호
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• pp.169-190
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• 2006

최근 가축에 있어서 DNA marker를 이용하여 경제적으로 유용한 유전자를 찾아내는 연구가 활발히 진행되고 있다. 소의 경우 생산성을 향상시키기 위하여 경제형질관련 양적 형질좌위에 존재하는 후보 유전자를 선발한 후 형질변이의 원인이 되는 염기서열을 찾아 표지인자로 이용을 하고 있다. 본 연구는 소의 중요 경제형질인 육질 및 육량과 관련된 분자 유전학적 연구와 더불어 경제형질관련 후보유전자를 찾아내기 위하여 최근에 많이 이용되고 있는 microarray에 대하여 고찰하였다. 특히 microarray의 경우 cDNA microarray에서 oligoarray를 제작하여 이용함으로서 실험의 오차를 최대한 줄이는 방향으로 연구가 진행되고 있다. 소의 형질 관련 유전자에 관한 연구는 bovine genome sequencing이 끝난 현 시점에서 연구의 속도가 가속화될 것으로 생각되며 경제형질 원인 유전자의 분석 뿐 아니라 질병 저항성과 환경에 영향을 많이 받는 유전자를 확인하여 선발에 이용하기 위한 연구가 계속될 것으로 생각된다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 춘계학술대회
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• pp.122-122
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• 2022

• Asian-Australasian Journal of Animal Sciences
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• 제31권8호
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• pp.1134-1140
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• 2018

Objective: Fatty acid composition is one of the most important meat quality traits because it can contribute to functional, sensorial, and nutritional factors. In this study, quantitative trait locus (QTL) analyses for fatty acid composition traits were investigated in thigh and breast meat of Korean native chicken (KNC). Methods: In total, 18 fatty acid composition traits were investigated from each meat sample using 83 parents, and 595 $F_1$ chicks of 20 week old. Genotype assessment was performed using 171 informative DNA markers on 26 autosomes. The KNC linkage map was constructed by CRI-MAP software, which calculated genetic distances, with map orders between markers. The half-sib and full-sib QTL analyses were performed using GridQTL and SOLAR programs, respectively. Results: In total, 30 QTLs (12 in the thigh and 18 in the breast meat) were detected by the half-sib analysis and 7 QTLs (3 in the thigh and 4 in the breast meat) were identified by the full-sib analysis. Conclusion: With further verification of the QTL regions using additional markers and positional candidate gene studies, these results can provide valuable information for determining causative mutations affecting the fatty acid composition of KNC meat. Moreover, these findings may aid in the selection of birds with favorable fatty acid composition traits.

• Mycobiology
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• 제49권6호
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• pp.589-598
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• 2021

White strains of Hypsizygus marmoreus are more difficult to cultivate than are brown strains; therefore, new white strain breeding strategies are required. Accordingly, we constructed the genetic map of H. marmoreus with 1996 SNP markers on 11 linkage groups (LGs) spanning 1380.49 cM. Prior to analysis, 82 backcrossed strains (HM8 lines) were generated by mating between KMCC03106-31 and the progenies of the F1 hybrid (Hami-18 × KMCC03106-93). Using HM8, the first 23 quantitative trait loci (QTLs) of yield-related traits were detected with high limit of detection (LOD) scores (1.98-9.86). The length, thickness, and hardness of the stipe were colocated on LG 1. Especially, length of stipe and thickness of stipe were highly correlated given that the correlation coefficients were negative (-0.39, p value ≤ .01). And a typical biomodal distribution was observed for lightness of the pileus and the lightness of the pileus trait belonged to the LG 8, as did traits of earliness and mycelial growth in potato dextrose agar (PDA) medium. Therefore, results for color traits can be suggested that color is controlled by a multi-gene of one locus. The yield trait was highly negatively correlated with the traits for thickness of the stipe (-0.45, p value ≤ .01). Based on additive effects, the white strain was confirmed as recessive; however, traits of mycelial growth, lightness, and quality were inherited by backcrossed HM8 lines. This new genetic map, finely mapped QTLs, and the strong selection markers could be used in molecular breeding of H. marmoreus.

• 응용통계연구
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• 제21권1호
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• pp.19-33
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• 2008

연속 형질(quantitative trait)에 영향을 미치는 유전자를 알아내기 위해 형제 쌍의 자료를 수집하여, 주로 이용되는 Haseman과 Elston (1972)의 최소제곱 회귀검정법으로 분석하는데 이는 단일 형질에 대한 분석법이다. 현실적으로 여러 형질들이 복잡하게 단일유전자 좌위(single locus)와 연관되어 있어 함께 수집하게 되는 경우에는, 이러한 연관된 여러 형질을 동시에 분석하는 유전연관성 검정법(linkage test)이 절실히 필요한 실정이다. Amos 등 (1990)은 주성분(principal component) 선형모형을 이용하여 Haseman과 Elston (1972)방법을 둘 이상의 형질의 다변량 분석법으로 확장시켰다. 그러나 이 검정방법은 통계량의 분포를 알 수 없기에 아직 제 1종 오류가 제대로 통제되지 못하는 문제를 가지고 있다. 본 논문에서는 이러한 다변량 형질 자료의 연관성검정에 있어 단일변량에 대한 비모수 추세검정법을 다변량 자료에 대한 분석법으로 확장시킨 통계량을 사용할 것을 제안한다. Amos 등 (1990)이 제안한 방법과 다변량 추세검정 통계량을 모의실험으로 생성한 연속형 형질자료에 적용하였을 때, 다변량 추세검정 통계량은 Amos 등 (1990) 방법에서의 여러 문제점이 발생되지 않을 뿐만 아니라 모의실험에서 제 1종 오류가 정해진 유의수준에 가까운 것을 확인하였고, 검정적이 더 높음을 볼 수 있었다.

• 한국가금학회지
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• 제42권4호
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• pp.315-325
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• 2015

최근 소비자의 소득 수준이 향상되고, 육제품의 다원화 성향이 증가하면서 가축개량은 과거 성장 및 육량 중심의 개량에서 품질 중심의 개량으로 중심이 이동하고 있다. 특히, 식육의 품질 중에서 육색은 소비자가 식육을 선택 및 질을 판단하는 기준으로 작용하기 때문에 매우 중요한 형질이라고 볼 수 있다. 경제적으로 유용한 형질은 대부분 측정가능한 연속변이에 해당하고, 이러한 형질은 대부분 여러 유전자가 형질에 영향을 미치는 양적형질 좌위(Quantitative Trait Loci;QTL)에 속한다. Chicken QTL db에 보고된 닭의 육색형질과 관련된 형질들은 육색(Bco, Mco), 가열감량(DL), pH가 보고되어 있으며, 이는 닭의 13개 염색체에서 33개 QTL 및 association 영역이 보고되고 있다. 이 중에서 육색관련 후보 유전자는 APP, BCMO1, COL1A2, FTO, KPNA2, PSMD12, G0S2, FTSJ3가 있으며, 가열감량관련 후보유전자는 AGRP, FTO, pH와 관련된 후보유전자는 GALNT1, PCDH19, DIAPH1, SPP2 유전자로 총 14개 유전자가 확인되었다. 이렇게 확인된 후보유전자 및 QTL 연구결과는 한국재래닭에 적용 및 활용 가능성을 확인해 볼 필요가 있으며, 이러한 적용은 낮은 성장속도의 단점을 가진 한국재래닭의 개발에 있어 품질의 개량속도를 높여 산업적 가치를 빠르게 끌어올릴 수 있는 중요한 표지인자가 될 수 있을 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제29권1호
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• pp.43-50
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• 2016

Quantitative trait locus (QTL) is a particular region of the genome containing one or more genes associated with economically important quantitative traits. This study was conducted to identify QTL regions for body weight and growth traits in purebred Korean native chicken (KNC). F1 samples (n = 595) were genotyped using 127 microsatellite markers and 8 single nucleotide polymorphisms that covered 2,616.1 centi Morgan (cM) of map length for 26 autosomal linkage groups. Body weight traits were measured every 2 weeks from hatch to 20 weeks of age. Weight of half carcass was also collected together with growth rate. A multipoint variance component linkage approach was used to identify QTLs for the body weight traits. Two significant QTLs for growth were identified on chicken chromosome 3 (GGA3) for growth 16 to18 weeks (logarithm of the odds [LOD] = 3.24, Nominal p value = 0.0001) and GGA4 for growth 6 to 8 weeks (LOD = 2.88, Nominal p value = 0.0003). Additionally, one significant QTL and three suggestive QTLs were detected for body weight traits in KNC; significant QTL for body weight at 4 weeks (LOD = 2.52, nominal p value = 0.0007) and suggestive QTL for 8 weeks (LOD = 1.96, Nominal p value = 0.0027) were detected on GGA4; QTLs were also detected for two different body weight traits: body weight at 16 weeks on GGA3 and body weight at 18 weeks on GGA19. Additionally, two suggestive QTLs for carcass weight were detected at 0 and 70 cM on GGA19. In conclusion, the current study identified several significant and suggestive QTLs that affect growth related traits in a unique resource pedigree in purebred KNC. This information will contribute to improving the body weight traits in native chicken breeds, especially for the Asian native chicken breeds.

• Asian-Australasian Journal of Animal Sciences
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• 제17권8호
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• pp.1033-1038
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• 2004

LOD scores related to marbling scores and permutation test have been applied for the purpose detecting quantitative trait loci (QTL) and we selected a considerable major locus BM4311. K-means clustering, for the major DNA marker mining of BM4311 microsatellite loci in Hanwoo chromosome 6, has been tried and five traits are divided by three cluster groups. Then, the three cluster groups are classified according to six DNA markers. Finally, bootstrap test method to calculate confidence intervals, using resampling method, has been adapted in order to find major DNA markers. It could be concluded that the major markers of BM4311 locus in Hanwoo chromosome 6 were DNA marker 100 and 95 bp.

• Molecules and Cells
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• 제37권2호
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• pp.149-160
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• 2014

Plant breeders have focused on improving plant architecture as an effective means to increase crop yield. Here, we identify the main-effect quantitative trait loci (QTLs) for plant shape-related traits in rice (Oryza sativa) and find candidate genes by applying whole genome re-sequencing of two parental cultivars using next-generation sequencing. To identify QTLs influencing plant shape, we analyzed six traits: plant height, tiller number, panicle diameter, panicle length, flag leaf length, and flag leaf width. We performed QTL analysis with 178 $F_7$ recombinant inbred lines (RILs) from a cross of japonica rice line 'SNU-SG1' and indica rice line 'Milyang23'. Using 131 molecular markers, including 28 insertion/deletion markers, we identified 11 main- and 16 minor-effect QTLs for the six traits with a threshold LOD value > 2.8. Our sequence analysis identified fifty-four candidate genes for the main-effect QTLs. By further comparison of coding sequences and meta-expression profiles between japonica and indica rice varieties, we finally chose 15 strong candidate genes for the 11 main-effect QTLs. Our study shows that the whole-genome sequence data substantially enhanced the efficiency of polymorphic marker development for QTL fine-mapping and the identification of possible candidate genes. This yields useful genetic resources for breeding high-yielding rice cultivars with improved plant architecture.

• Communications for Statistical Applications and Methods
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• 제11권3호
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• pp.657-668
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• 2004

Permutation test has been applied for the QTL(quantitative trait loci) analysis and we selected a major locus. K -means clustering analysis, for the major DNA Marker mining of ILSTS035 microsatellite loci in Hanwoo chromosome 6, has been described. Finally, bootstrap testing method has been adapted to calculate confidence intervals and for finding major DNA Markers.