• Industrial Engineering and Management Systems
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• 제14권4호
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• pp.392-400
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• 2015

Monitoring of plasma etch processes for fault detection is one of the hallmark procedures in semiconductor manufacturing. Optical emission spectroscopy (OES) has been considered as a gold standard for modeling plasma etching processes for on-line diagnosis and monitoring. However, statistical quantitative methods for processing the OES data are still lacking. There is an urgent need for a statistical quantitative method to deal with high-dimensional OES data for improving the quality of etched wafers. Therefore, we propose a robust relevance vector machine (RRVM) for regression with statistical quantitative features for modeling etch rate and uniformity in plasma etch processes by using OES data. For effectively dealing with the OES data complexity, we identify seven statistical features for extraction from raw OES data by reducing the data dimensionality. The experimental results demonstrate that the proposed approach is more suitable for high-accuracy monitoring of plasma etch responses obtained from OES.

• Journal of Microbiology
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• 제41권4호
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• pp.320-326
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• 2003

In order to identify Vibrio vulnificus in the Yellow Sea near Gunsan, Korea during the early and late summers, the efficiency of the real-time quantitative TaqMan PCR was compared to the efficiency of the conventional PCR and Biolog identification system^TM. Primers and a probe were designed from the hemolysin/cytolysin gene sequence of V. vulnificus strains. The number of positive detections by real-time quantitative TaqMan PCR, conventional PCR, and the Biolog identification system from seawater were 53 (36.8%), 36 (25%), and 10 strains (6.9%), respectively, among 144 samples collected from Yellow Sea near Gunsan, Korea. Thus, the detection method of the real-time quantitative TaqMan PCR assay was more effective in terms of accuracy than that of the conventional PCR and Biolog system. Therefore, our results showed that the real-time TaqMan probe and the primer set developed in this study can be applied successfully as a rapid screening tool for the detection of V. vulnificus.

• KSBB Journal
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• 제29권5호
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• pp.361-371
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• 2014

Mycoplasma is well recognized as one of the most prevalent and serious microbial contaminants of biologic manufacturing processes. Conventional methods for mycoplasma testing, direct culture method and indirect indicator cell culture method, are lengthy, costly and less sensitive to noncultivable species. In this report, we describe a new TaqMan probe-based real-time PCR method for rapid and quantitative detection of mycoplasma contamination during manufacture of biologics. Universal mycoplasma primers were used for mycoplasma PCR and mycoplasma DNA was quantified by use of a specific TaqMan probe. Specificity, sensitivity, and robustness of the real-time PCR method was validated according to the European Pharmacopoeia. The validation results met required criteria to justify its use as a replacement for the culture method. The established real-time PCR assay was successfully applied to the detection of mycoplasma from human keratinocyte and mesenchymal stem cell as well as Vero cell lines artificially infected with mycoplasma. The overall results indicated that this rapid, specific, sensitive, and robust assay can be reliably used for quantitative detection of mycoplasma contamination during manufacture of biologics.

• Journal of Microbiology and Biotechnology
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• 제11권2호
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• pp.199-203
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• 2001

A simple quantitative method to measure the degree of silanization was developed, based on the reaction of ninhydrin with the silanization reagent (3-aminopropyltriethoxysilane, 3-APTES). At low concentrations (0.001-0.005%, v/v) of 3-APTES, a good linearity was obtained when 3-APTES reacted with undiluted ninhydrin for 30 min. On the other hand, at high levels of 3-APTES, a linearity was obtained when 3-APTES reacted with 3-fold diluted ninhydrin for 20 min. The reliability of regression curves mentioned above was expressed as a regression coefficient ($R^2$) of more than 0.99. Immobilization of different enzymes was introduced via silanization by using the 3-APTES in order to confirm the validity of the ninhydrin method. When yield for each step in the immobilizatio process were compared, yields of both glutaraldehyde and protein were founc to have the same tendency to silanization. These results shw that the ninhydrin method was suitable for quatitative analysis of silanization and that yields of immobilization could be pre-estimated by measuring silanization levels using the ninhydrin method.

• 부모자녀건강학회지
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• 제14권1호
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• pp.1-8
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• 2011

Purpose: This study was aimed to classify the major subjects and theme and to analyze the data collection and analysis method in research papers published in the Korean Parent-Child Health Journal of the Academic Society of Parent-Child Health since 1998. Methods: A total 152 studies published from the first edition to volume 12, number 2 were reviewed using structured analysis criteria developed by researchers; research type, research design, research subjects, research theme, data collection and analysis method. Research theme was founded 4 nursing domains. Data collection and analysis method of papers were limited to quantitative and qualitative researches. Results: One hundred papers conducted quantitative research; 79.0% used survey design. Most of the data collection and analysis method in quantitative research were self-reported questionnaire (69.4%) and parametric statistics respectively. The research subjects of sixty three papers were parent with well or child. The common domain studies was human related concepts such as raring. Conclusion: The findings of this study suggest that published studies have been improved and diversified, however, detailed and clear evaluation tool that assess study process and method should be developed as a way to further improve the quality of published papers in the Korean Parent-Child Health Journal.

• 전기전자학회논문지
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• 제17권1호
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• pp.1-9
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• 2013

3차원 영상 해석 기법에 의해 구해진 DEM(Digital Elevation Map)을 정량적으로 평가하는 것은 영상 해석 기법의 유효성을 검증하기 위해 매우 중요하다. 본 논문에서는 모의 영상 제작에 의한 3차원 재구성 과정의 새로운 정량적 평가 방법을 제안하였다. 제안된 방법은 미리 확보된 DEM과 정사영상(ortho-image)을 가상의 실제 값(pseudo ground truth)으로 가정한 것에 의한 것이다. 제안된 방법의 과정은 그래픽스에서 사용되는 ray tracing 알고리즘을 구성하여 가상의 실제 값에 적용함으로서 원하는 시점으로부터의 한 쌍의 모의 영상을 제작하는 것으로부터 시작된다. 제작된 모의 영상 쌍으로부터 구해진 DEM을 가상의 실제 값과 비교하면 구해진 DEM의 정량적인 오차 분석이 가능하여, 적용된 3차원 영상 해석 기법의 유효성이 평가될 수 있다. 제안된 평가 방법의 타당성을 검증하기 위해, 정량적 및 정성적인 실험이 수행되었다. 이를 위해 발생되는 모의 영상이 실제 형상을 재현하는 정도를 정량적인 수치로서 구하여 제안된 방법을 타당성을 입증하였다. 또한 정합창의 크기 변화에 따른 DEM의 정확도를 제안된 평가 방법에 의해 실험하였다. 이러한 실험 결과가 예견된 결과와 일치함에 의해 제안된 평가 방법의 타당성을 정성적으로도 명백히 증명하였다.

• Journal of the Korean Data and Information Science Society
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• 제18권4호
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• pp.1057-1064
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• 2007

From the view of natural language process, quantitative linguistic analysis is a linguistic study relying on statistical methods, and is a mathematical linguistics in an attempt to discover various linguistic characters by interpreting linguistic facts quantitatively through statistical methods. In this study, I would like to introduce a quantitative linguistic analysis method utilizing a computer and statistical methods on literary works. I also try to introduce a use of SynKDP, a synthesized Korean data process, and show the relations between distribution of linguistic unit elements which are used by the hero in a novel #Sassinamjunggi# and theme analysis on literary works.

• 대한자기공명의과학회:학술대회논문집
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• 대한자기공명의과학회 2001년도 제6차 학술대회 초록집
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• pp.165-165
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• 2001

Purpose： To obtain quantitative diffusion-tensor anisotropy information of human cerebral structu using turbo STEAM diffusion-tensor imaging. Method： Quantitative diffusion anisotropy MR images were obtained in 7 healthy adults using turbo STEAM sequence and a combination of tetrahedral and orthogonal diffusi gradients. Both relative anisotropy(RA) and fractional anisotropy(FA) values were measured various brain regions. The anisotropy index was then compared with the reported valu resulting from EPI-based diffusion tensor imaging.

• 대한자기공명의과학회:학술대회논문집
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• 대한자기공명의과학회 2001년도 제6차 학술대회 초록집
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• pp.138-138
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• 2001

Purpose： To obtain quantitative diffusion-tensor anisotropy information of human cerebral structu using turbo STEAM diffusion-tensor imaging. Method： Quantitative diffusion anisotropy MR images were obtained in 7 healthy adults using turbo STEAM sequence and a combination of tetrahedral and orthogonal diffusi gradients. Both relative anisotropy(RA) and fractional anisotropy(FA) values were measured various brain regions. The anisotropy index was then compared with the reported valu resulting from EPI-based diffusion tensor imaging.

• Archives of Pharmacal Research
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• 제17권2호
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• pp.76-79
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• 1994

The concentration of styrne in blood of the occupationally syrene-exposed workers was checked by gas chromatographic headspace analysis. Mandelic acd in urine, that is a major metabolite of styrene, and hippuric acid wre also analyzed by high performance liquid chromatography. For the biological monitoring of styrene-exposed workers, the routine method of the quantitative determination of styrene nad its metabolites in the biolgical samples were studied.