• 한국산림과학회지
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• 제105권2호
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• pp.167-176
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• 2016

본 연구는 울산광역시에 위치한 봉대산을 대상으로 산불피해 4년 후 인공조림 활엽수임분(튤립나무, 왕벚나무, 상수리나무조림지), 미복구지, 인접 미피해 소나무임분의 유기물층 및 토양층의 이화학적 특성과 조림목의 잎 내 양분함량을 조사하였다. 유기물층의 탄소 축적량은 미피해 소나무임분이 $9388kg{\cdot}C{\cdot}ha^{-1}$로, 활엽수임분 $2394{\sim}3551kg{\cdot}C{\cdot}ha^{-1}$ 나, 미복구지 $3689kg{\cdot}C{\cdot}ha^{-1}$ 에 비해 유의적으로(P < 0.05) 높았다. 그러나 인, 칼슘, 마그네슘 축적량은 활엽수임분과 미피해 소나무임분 사이에 유의적인 차가 없었다(P > 0.05). 토양 이화학적 특성 중 왕벚나무조림지의 액상과 토양 pH는 18.4%와 pH 4.80로, 미피해 소나무임분 7.0%와 토양 pH 4.10에 비해 유의적으로 높았다. 토양 유기탄소함량은 인공조림 활엽수임분이 1.89~3.60%로 미복구지 1.41%에 비해 증가하는 경향을 보이나 유의적인 차는 없었다. 토양 칼슘과 마그네슘 축적량의 경우 튤립나무와 왕벚나무조림지가 미피해 소나무임분과 미복구지에 비해 유의적으로 높은 값을 보였다. 잎 내 양분함량 중 칼슘이나 마그네슘함량은 튤립나무(칼슘: 1.10%; 마그네슘: 0.37%)가 상수리나무(칼슘: 0.50%; 마그네슘: 0.14%)나 인접 미피해 소나무 잎(칼슘: 0.33%; 마그네슘: 0.10%)에 비해 유의적으로 높게 나타났다. 본 연구결과에 따르면 산불발생 4년 이내 초기단계 결과이지만, 유기물층의 인, 칼슘, 마그네슘축적량은 미피해 산림지역과 유의적인 차이는 없었으나, 토양층의 칼슘과 마그네슘 축적량은 식재수종의 영향이 큰 것으로 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제29권5호
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• pp.674-680
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• 2016

This study investigated the effects of feeds naturally contaminated with mycotoxins on growth performance, serum biochemical parameters, carcass traits, and splenic heat shock protein 70 (Hsp70) mRNA expression levels in broiler chickens. The efficacy of yeast cell wall (YCW) adsorbent in preventing mycotoxicosis was also evaluated. Three hundred 1-d-old Arbor Acres broiler chicks were randomly allotted to 3 treatments in completely randomized design for 42 d. Each treatment group had 5 replicate pens with 20 birds. The treatments were as follows: i) basal diet (control), ii) naturally contaminated diet (NCD), and iii) NCD+0.2% YCW adsorbent (NCDD). The NCD decreased average daily gain (ADG) (p<0.01) of 0 to 21 d, 22 to 42 d, and 0 to 42 d, and increased feed conversion ratio (p<0.01) of 22 to 42 d and 0 to 42 d. Both the breast meat percentage and thigh meat percentage of the NCD group were significantly higher (p<0.01) than that of the control group on d 21. The NCD group showed significantly increased levels of triglycerides (p<0.05) and cholesterol (p<0.05) on both d 21 and d 42 compared to the control group. However, the NCD significantly reduced (p<0.01) the high-density lipoprotein (HDL) on d 42 compared to controls. Compared with the NCD, supplementation with YCW significantly improved (p<0.01) the ADG of 0 to 21 d and 0 to 42 d, and increased (p<0.01) concentrations of HDL on d 42, and on d 21, and triglycerides (p<0.05) on d 21 and d 42. Supplementation with YCW reduced (p<0.01) the breast meat percentage, the thigh meat percentage, the concentrations of cholesterol (p<0.01) and the low-density lipoprotein (p<0.05) on d 21, and improved (p<0.01) the splenic Hsp70 mRNA expression levels compared with the NCD group. The results of this study indicated that feeding NCD for 42 d had adverse effects on broiler chickens, and that YCW might be beneficial in counteracting the effects of mycotoxins.

• 벤처창업연구
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• 제9권1호
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• pp.153-164
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• 2014

R&D는 산업과 제품의 특성에 따라 그 중요도가 다르며, 제품의 고부가가치를 중요시하는 첨단산업에 있어서 R&D의 중요성이 크다. 그런데 첨단산업의 기업은 전통적인 상장기업에 비해 재무변수의 기업가치 설명력이 상대적으로 낮게 나타나는데 그 원인은 재무제표에 기록되지 않은 무형자산 때문인 것으로 추정된다. 이에 본 연구의 목적은 코스닥기업을 첨단산업과 비첨단산업으로 나누어 무형자산성 지출의 가치관련성을 분석하는 것이다. 무형자산성 지출은 연구개발비, 교육훈련비, 광고선전비로 측정하였다. 분석 결과 첫째, 첨단산업에서는 연구개발비가 기업가치에 양(+)의 유의한 영향을 미치는 것으로 나타났고 교육훈련비는 기업가치에 음(-)의 유의한 영향을 미치는 것으로 나타났다. 비첨단산업에 있어서는 교육훈련비가 기업가치에 양(+)의 유의한 영향을 미치는 것으로 나타났다. 첨단산업과 비첨단산업 모두 광고선전비는 유의한 영향을 미치지 않는 것으로 나타났다. 둘째, 대형회계법인의 감사품질은 첨단산업의 연구개발비의 가치관련성을 더욱 증가시키고 비첨단산업의 교육훈련비의 가치관련성을 증가시키는 역할을 하는 것으로 나타났다. 이러한 연구결과는 투자자들이 전자, 통신, 제약, 정밀기계와 같은 첨단산업군의 연구개발비 투자에 대해 비첨단산업군의 연구개발비 투자와 비교해서 더 가치 있는 투자로 평가하는 것으로 해석할 수 있다. 본 연구의 연구결과는 첨단산업과 비첨단산업의 경영자의 무형자산성 투자의 의사결정에 있어서 유익한 참고가 될 수 있을 것으로 기대한다.

• The Korean Journal of Physiology and Pharmacology
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• 제5권4호
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• pp.287-297
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• 2001

Contraction of smooth muscle is initiated by an increase in cytosolic $Ca^{2+}$ leading to activation of $Ca^{2+}$/ calmodulin-dependnet myosin light chain (MLC) kinase and phosphorylation of MLC. The types of contraction and signaling mechanisms mediating contraction differ depending on the region. The involvement of these different mechanisms varies depending on the source of $Ca^{2+}$ and the kinetic of $Ca^{2+}$ mobilization. $Ca^{2+}$ mobilizing agonists stimulate different phospholipases $(PLC-{\beta},\;PLD\;and\;PLA_2)$ to generate one or more $Ca^{2+}$ mobilizing messengers $(IP_3\;and\;AA),$ and diacylglycerol (DAG), an activator of protein kinase C (PKC). The relative contributions of $PLC-{\beta},\;PLA_2$ and PLD to generate second messengers vary greatly between cells and types of contraction. In smooth muscle cell derived form the circular muscle layer of the intestine, preferential hydrolysis of $PIP_2$ and generation of $IP_3$ and $IP_3-dependent\;Ca^{2+}$ release initiate the contraction. In smooth muscle cells derived from longitudinal muscle layer of the intestine, preferential hydrolysis of PC by PLA2, generation of AA and AA-mediated $Ca^{2+}$ influx, cADP ribose formation and $Ca^{2+}-induced\;Ca^{2+}$ release initiate the contraction. Sustained contraction, however, in both cell types is mediated by $Ca^{2+}-independent$ mechanism involving activation of $PKC-{\varepsilon}$ by DAG derived form PLD. A functional linkage between $G_{13},$ RhoA, ROCK, $PKC-{\varepsilon},$ CPI-17 and MLC phosphorylation in sustained contraction has been implicated. Contraction of normal esophageal circular muscle (ESO) in response to acetylcholine (ACh) is linked to $M_2$ muscarinic receptors activating at least three intracellular phospholipases, i.e. phosphatidylcholine-specific phospholipase C (PC-PLC), phospholipase D (PLD) and the high molecular weight (85 kDa) cytosolic phospholipase $A_2\;(cPLA_2)$ to induce phosphatidylcholine (PC) metabolism, production of diacylglycerol (DAG) and arachidonic acid (AA), resulting in activation of a protein kinase C (PKC)-dependent pathway. In contrast, lower esophageal sphincter (LES) contraction induced by maximally effective doses of ACh is mediated by muscarinic $M_3$ receptors, linked to pertussis toxin-insensitive GTP-binding proteins of the $G_{q/11}$ type. They activate phospholipase C, which hydrolyzes phosphatidylinositol bisphosphate $(PIP_2),$ producing inositol 1, 4, 5-trisphosphate $(IP_3)$ and DAG. $IP_3$ causes release of intracellular $Ca^{2+}$ and formation of a $Ca^{2+}$-calmodulin complex, resulting in activation of myosin light chain kinase and contraction through a calmodulin-dependent pathway.

• Journal of Hospice and Palliative Care
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• 제14권3호
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• pp.152-162
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• 2011

목적: 본 연구는 우리나라 호스피스 간호 연구 동향을 알아보기 위해 시도되었다. 방법: 본 연구의 대상은 1985년부터 2009년까지 24년 동안 발표된 국내 호스피스 간호 관련 논문 총 183편으로 간호학위논문 62편과 비학위논문으로서 학술지에 게재된 논문 121편이었다. 본 분석대상 논문은 한국교육학술정보원에서 제공하는 학술정보서비스(http://www.riss.kr)를 이용하여 호스피스(완화) 간호, 말기(암)환자의 핵심어를 조합하여 검색하였다. 검색결과 호스피스 관련 국내 학위논문과 대한간호학회지, 아동간호학회지, 정신간호학회지, 성인간호학회지, 병원간호사회지, 행정간호학회지, 종양간호학회지, 한국 호스피스 완화의료학회지, 호스피스 학술지, 호스피스 논집 등에 게재된 논문들을 대상으로 하였고, 학위논문 중 학술지에 게재된 것은 학술지를 분석대상으로 하였다. 단, 종설과 인터넷으로 원문수집이 불가능한 논문은 제외하였다. 결과: 호스피스 간호 관련 논문 수가 급증하였고, 연구설계 유형은 양적연구가 질적연구보다 월등하게 많았다. 분석대상 논문에서 도출된 주요어는 5개 영역에서 총 74개가 도출되었고, 총 225개의 주요어를 사용하였다. 자료수집시 윤리적 고려는 서면동의가 많았고, IRB를 통과한 연구가 극소수이며, 다학제간 연구 지원금은 점차 증가하였다. 결론: 결론적으로 말기암환자의 간호문제나 현상을 연구하기 위해서는 조사연구보다는 질적연구 방법의 접근이 많이 필요하고, 연구개념도 삶의 질과 더불어 죽음의 질에 대한 연구가 필요하며, 중재연구에 있어서는 말기암환자의 가장 큰 문제로 대두되고 있는 통증완화, 기타 주요 신체간호, 그리고 보완대체요법에 관한 간호연구가 더 필요하다고 본다. 또한 간호학 연구를 넘어 다학제적 팀 연구가 더 많이 수행되어야 하며, 마지막으로 아동호스피스에 관한 연구가 매우 미비한데, 이에 관한 연구가 더 시행되어야함을 제안한다.

• 생물정신의학
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• 제2권1호
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• pp.3-19
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• 1995

Numerous investigators have conducted extensive investigation in the search for biological markers in psychiatric illness. There are, as a test of q biological approach to the diagnosis of the psychiatric illness, tests for the neurotransmitters, their metabolites, and related enzymes, the neurotransmitter receptors, the neuroendocrine output and response, the membrane transport, peptides and eletrolytes. They are called the biological markers, and they are helpful for the diagnosis or differential diagnosis, choice of treatment or drugs, symptom improvement, predictor of recurrence and anticipation of suicidal attempt. These studies are among the main purposes that are pursued in the neuroscience and based on the potential utility of the biological markers mentioned above. Since 1970's, lots 01 biological markers' studies for the diagnosis, differential diagnosis or subtypes differentiation have been done but varieties of different opinions have been drawn since then through they could explain the charaters of main psychiatric illness(especially schizophrenia and mood disorder). But, the search for biological markers, including displines of neuroendoclinology and neurochemistry(neurotransmitter and thair metabolite), has yielded a number of putative trait merkers and state markers for psychayric illness. This paper aims to anticipate or evaluate the good response to the therapy(Therpeutic response) with lots of markers. Acoording to the diagnosis of lots of diseases or subtypes, we are going to review the papers, mainly concern with 'Is there any Marker' or 'Is any test possible to detect the improvement clinically?' 'Is it possible to predict the recurrence or good prognsis?' or 'Is it possible to select any drug or therapy to bring the good response?' The biological tests to review are mainly the metabolites of catecholamine neurotransmitter, and especially neuroendocrine test based on the knowledge that hormons of the adenohypophysis are influenced by activity of the cerebral or limbic neurons as well as the hypothalamus ones. Among them, author introduced some clinically available tests that are DST, TRH stimulation test(TRHST), GH stimulation test, and the urine MHPG test that can give us the evaluation of the treatment response, the predictor for recurrence or choice of drug that can bring a good response. So author discussed thair potential utility in clarifying, therapeutic, and prognostic issues in psychatric illness. We hope they'll be used and look forward to more active study on the different opinion.

• 한국식품과학회지
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• 제50권5호
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• pp.555-563
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• 2018

보리는 높은 비율로 식이섬유를 함유하고 있어, 일반적으로 영양학적으로는 전곡 형태로의 섭취가 추천되고 있지만, 조직감과 소화율을 고려하여 발효물 형태의 소비가 바람직한 것으로 판단되고 있다. 본 연구는 효소당화 후, 효모 및 유산균 발효를 거쳐 조제한 보리발효물로부터 조다당 BF-CP를 분리하고, 대식세포에 대한 면역증강 효과 및 세포 내 신호전달을 규명하여 기능성 소재로의 이용방안을 모색하기 위해 계획되었다. BF-CP 획분의 일반화학적 특성을 분석한 결과 70.7% 글루코스 11.4% 자일로스 및 9.0% 아라비노스를 포함하여 91.1%의 중성당으로 이루어진 중성다당이었다. BF-CP는 RAW 264.7 대식세포주에서 농도의존적으로 IL-6, $TNF-{\alpha}$와 같은 사이토카인 및 NO의 생산능을 유도하는 등 높은 대식세포 활성능을 나타냈다. 또한 qPCR 분석을 통해, BF-CP 획분을 대식세포주에 처리하였을 때, 처리 농도에 비례하여 IL-6, $TNF-{\alpha}$ 및 iNOS의 mRNA 유전자 발현을 증가시킴을 확인할 수 있었다. 한편 Western blot을 활용한 신호전달 단백질 추적실험에서 BF-CP 획분을 대식세포주에 처리하였을 때, JNK, ERK 및 p38과 같은 MAPK 경로와 $NF-{\kappa}B$ 경로의 관련 단백질을 인산화시킴이 확인되었으며 그 활성은 BF-CP 농도에 의존적이었다. 이상의 결과로부터 보리발효물 유래 다당 BF-CP는 MAPK와 $NF-{\kappa}B$ 경로를 통해 대식세포를 활성화시키며, 이를 통하여 NO, IL-6 및 $TNF-{\alpha}$와 같은 면역활성화 관련 물질의 생산을 높은 비율로 유도시킨다는 것을 최종 확인할 수 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권5호
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• pp.638-644
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• 2006

Sixteen adult male buffaloes (average body weight $443{\pm}14kg$) were equally distributed into four groups in an experiment to study the effect of supplementary protein sources on energy utilization efficiency in buffaloes fed a wheat straw-based diet. The animals in the control group were offered a basal diet composed of 700 g deoiled ground nut cake and ad libitum wheat straw. Animals of other groups were offered 1.8 kg of soyabean meal (SBM), linseed meal (LSM) or mustard cake (MC) along with the basal diet. Protein supplementation increased the digestibility of DM (p<0.01), OM (p<0.01) CP (p<0.01) and CF (p<0.05). Maximum CP digestibility was observed on SBM, followed by LSM and MC when compared to the control. Total DMI and DOMI was significantly (p<0.01) higher in protein supplemented groups with no differences between treatment groups. Digestible crude protein (DCP) intake and N balance were significantly (p<0.01) different between the groups; maximum response was obtained with SBM supplementation, followed by LSM and MC. Faecal energy was significantly (p<0.01) lower in SBM and LSM groups in comparison to other groups. Methane production (% DEI) was significantly (p<0.05) lower on the SBM treatment. Metabolizable energy (ME) intake increased significantly due to protein supplementation. Metabolizable energy intake (MEI) of animals in the MC group was less than LSM and SBM. Energy balance was increased significantly (p<0.01) due to protein supplementation and within supplement variation was also significant with maximum balance in SBM followed by LSM and MC groups. Protein supplementation significantly (p<0.05) increased the digestibility and metabolizability of energy from whole ration. Metabolizable energy (ME) content (Mcal/kg DM) of SBM, LSM and MC was 4.49, 3.56 and 2.56, respectively. It was concluded that protein supplementation of wheat straw increased intake, digestibility and metabolizability of energy and maximum response could be obtained when soybean meal was used as a supplement.

• Journal of Animal Science and Technology
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• 제57권3호
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• pp.8.1-8.8
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• 2015

Background: The demand for healthy, lean and consistent meat products containing low saturated fatty acid content and high quality polyunsaturated fatty acids (PUFA), especially long-chain (${\geq}C_{20}$) omega-3 PUFA, has increased in recent times. Fat deposition is altered by both the genetic background and dietary supplements, and this study aimed to assess the effect of dietary Spirulina supplementation levels on the mRNA expression patterns of genes controlling lipid metabolism in the subcutaneous adipose tissue (SAT) and Longissimus dorsi (ld) muscle of Australian crossbred sheep. Methods: Twenty-four weaned lambs belonging to four breeds under the same management conditions were maintained on ryegrass pasture and fed three levels of Spirulina supplement (control, low and high). In terms of nutrient composition, Spirulina is a nutrient-rich supplement that contains all essential amino acids, vitamins and minerals. It also is a rich source of carotenoids and fatty acids, especially gamma-linolenic acid (GLA) that infer health benefits. After slaughter, subcutaneous adipose tissue (SAT) and ld samples were subjected to mRNA extraction and reverse transcription using quantitative polymerase chain reaction (RT-qPCR) to assess the mRNA expression levels of the Aralkylamine N-acetyltransferase (AANAT), Adrenergic beta-3 receptor (ADRB3), B-cell translocation gene 2 (BTG2) and Fatty acid synthase (FASN) genes, which are associated with lipid metabolism. Results: Both low and high Spirulina supplementation levels strongly up-regulated the transcription of all the selected genes in both SAT and ld tissues (mostly in the subcutaneous adipose), but sheep breed and sex did not influence the gene expression patterns in these tissues. Conclusions: The evidence indicates that high Spirulina supplementation level resulted in a decrease in intramuscular fat content in Australian purebred and crossbred sheep due to the enhanced production of melatonin in sheep muscle tissues and strong up-regulation of mRNA expression of BTG2 in SAT which negatively affected fat deposition. In contrast, low Spirulina supplementation level strongly up-regulated the ADRB3 and FASN genes responsible for fat production. These findings are consistent with the observed phenotypic data suggesting that low Spirulina supplementation level can increase lamb production, with higher long-chain PUFA content.

• 한국환경과학회지
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• 제24권11호
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• pp.1501-1511
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• 2015

As essential trace elements, copper and zinc play important roles in many physiological events in plants. In excess, however, these elements can limit plant growth. This study selected a heavy metal-tolerant plant by analyzing seed germination and biomass of alfalfa (Medicago sativa), canola (Brassica campestris subsp. napus var. nippo-oleifera), Chinese corn (Setaria italica), and a sorghum-sudangrass hybrid (Sorghum bicolor ${\times}$ S. sudanense), and determined heavy metal uptake capacity by analyzing biomass, chlorophyll a fluorescence, and heavy metal contents under high external copper or zinc levels. The seed germination rate and biomass of the sorghum-sudangrass hybrid were higher under copper or zinc stress compared to the other three plants. The plant biomass and photosynthetic pigment contents of the sorghum-sudangrass hybrid seedlings were less vulnerable under low levels of heavy metals (${\leq}50ppm$ copper or ${\leq}400ppm$ zinc). The maximum quantum yield of PSII ($F_v/F_m$) and the maximum primary yield of PSII ($F_v/F_o$) decreased with increasing copper or zinc levels. Under high copper levels, the decline in $F_v/F_m$ was caused only by the decline in $F_m$, and was accompanied by an increase in non-photochemical quenching (NPQ). The $F_v/F_m$ declined under high levels of zinc due to both a decrease in the maximum fluorescence ($F_m$) and an increase in the initial fluorescence ($F_o$), and this was accompanied by a marked decrease in photochemical quenching (qP), but not by an increase in NPQ. Accumulations of copper and zinc were found in both aboveand below-ground parts of plants, but were greater in the below-ground parts. The uptake capacity of the sorghum-sudangrass hybrid for copper and zinc reached 4459.1 mg/kg under 400 ppm copper and 9028.5 mg/kg under 1600 ppm zinc. Our results indicate that the sorghum-sudangrass hybrid contributes to the in situ phytoremediation of copper or zinc polluted soils due to its high biomass yield.