• BMB Reports
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• 제32권2호
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• pp.133-139
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• 1999

Thioltransferase, also known as glutaredoxin, was previously purified and characterized from Chinese cabbage (Brassica campestris ssp. napus var. pekinensis). However, in the process of gel filtration on Sephadex G-75, there were two activity peaks. In this study, a second thioltransferase (TTase CC-2) in the minor peak of the Sephadex G-75 elution profile was further purified using affinity chromatography on an S-hexylglutathione-agarose column by eluting with buffer solution containing 2.5 mM S-hexylglutathione. It showed a single band on SDS-PAGE indicating that TTase CC-2 is electrophoretically homogeneous. The molecular weight of TTase CC-2 was estimated to be about 22,000 daltons, and its isoelectric point was determined to be 6.73. Its size appears to be atypical and much larger than that of the first thioltransferase (TTase CC-1) from Chinese cabbage, and it can utilize 2-hydroxyethyl disulfide, S-sulfocysteine, and insulin as substrates. S-sulfocysteine was found to be a superior substrate for TTase CC-2. TTase CC-2 also displayed the reducing activity for non-disulfides such as dehydroascorbic acid. Its optimum pH was 8.5, which was consistent with that of TTase CC-1. TTase CC-2 activity was greatly activated by L-cysteine and reduced glutathione, and was found to be less heat-stable compared with TTase CC-1. Molecular and physiological differences between TTase CC-1 and TTase CC-2 remain to be elucidated. Chinese cabbage is the first plant which is known to contain two kinds of thioltransferases.

• 한국산림과학회지
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• 제103권2호
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• pp.240-247
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• 2014

본 연구는 황폐계류 복원지의 식생변화에 대한 시계열 분석을 통하여 복원기법 및 효과에 대해 고찰하고자 수행 되었으며, 수질정화습지와 월류보, 수생식물 복원지에서 복원 전, 후 식생변화를 2년간 모니터링 하였다. 복원 1년 후 월류보에서 초본류 증가와 수질정화습지에서 줄풀의 피복도 증가가 조사되었다. 식생 피복률은 복원직후 10~30% 감소되었고, 복원 1년 후 복원 전 수준으로 조사 되었다. 황폐계류 복원지의 식생변화는 복원 후 1년 경과시점부터 서식종수, 귀화률, 피복률이 증가하는 것으로 조사되었다.

• 한국산학기술학회논문지
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• 제8권2호
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• pp.380-384
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• 2007

COD 제거효율은 각 조의 부처꽃을 제외한 대조군의 경우 물상추 56%, 달뿌리풀 48%, 미나리 41%의 효율을 보이고, 발포유리가 첨가된 조의 경우는 물상추 69%, 달뿌리풀 68% 미나리 62%의 제거효율을 보임으로써 발포유리를 첨가한 조의 COD 제거효율이 대조군 보다 높았다. T-N의 제거효율은 발포유리를 첨가한 조의 경우 부처꽃 91%, 달뿌리풀 93%와 발포유리를 첨가한 혼합조가 94%의 높은 T-N 제거효율 보임으로써 모든 질소성분이 제거된 것으로 사료된다. T-P의 제거효율은 대조군의 부처꽃 35%, 달뿌리풀 8%이며, 발포유리를 첨가한 조의 부처꽃 78%, 달뿌리풀 43%로 발포유리를 첨가한조의 T-P 제거효율이 대조군 보다 높았다.

• BMB Reports
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• 제38권5호
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• pp.526-532
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• 2005

A lectin with in-vitro anticancer activity against established human cancer cell lines has been purified by affinity chromatography on asialofetuin-linked amino activated silica beads from the tubers of Arisaema tortuosum, popularly known as Himalayan Cobra lily, a monocot plant from the family Araceae. The bound Arisaema tortuosum lectin (ATL) was eluted with glycine-HCl buffer, pH 2.5. ATL was effectively inhibited by asialofetuin, a complex desialylated serum glycoprotein as well as by N-acetyl-D-lactosamine, a disaccharide. It gave a single band corresponding to a subunit molecular weight of 13.5 kDa in SDS-PAGE, pH 8.8 both under reducing and non reducing conditions. When subjected to gel-filtration on Biogel P-200, it was found to have a molecular weight of 54 kDa, suggesting a homotetramer structure, in which individual polypeptides are not bound to each other with disulfide bonds. ATL is a glycoprotein with 0.9% carbohydrate content, stable up to $55^{\circ}C$ and at pH 2 to 10. The lectin had no requirement for divalent metal ions i.e. $Ca^{2+}$ and $Mn^{2+}$ for its activity. However, as reported for other monocot lectins, ATL gave multiple bands in isoelectric focusing and Native PAGE, pH 8.3. The lectin was found to inhibit in vitro proliferation of human cancer cell lines HT29, SiHa and OVCAR-5.

• 청정기술
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• 제9권1호
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• pp.29-35
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• 2003

기존의 Epichlorohydrin의 생산 공정을 개선하기 위해 중간 생성물인 dichlorohydrin생성 반응인 hypochlorination 반응에 대한 실험을 통해 개선방안을 조사하였다. 첫 번째로 이 반응에서 사용되고 있는 공업용수를 PVC 공장으로부터 방출된 폐수를 재활용하여 사용하였을 경우 반응 수율에 미치는 영향에 대해 실험하였고 실제적인 반응 생성물 조성에는 크게 영향을 미치지 않는 것으로 나타났다. 부반응물 중 거의 대부분을 차지하고 있는 TCPA를 정제공정 이전에 "추출제 A"를 사용하여 제거할 수 있다. 이를 이용하여 TCPA에 의한 부가적인 부반응을 억제하고 후단의 정제공정에서 분리하는데 소요되는 에너지를 절감할 수 있을 것이다. 또한 Allyl chloride와 반응하는 염소기체의 양을 감소시키면서 생성물의 조성변화를 관찰하였다. 주반응 생성물의 수율에 는 거의 영향을 미치지 않으면서 부반응물의 비율이 다소 감소하는 경향을 확인할 수 있었다.

• Applied Biological Chemistry
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• 제35권4호
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• pp.300-307
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• 1992

파인애플의 과육과 줄기로부터 fruit-bromelain을 정제도 18배와 수율 23%, stem-bromelain을 정제도 46배와 수율 23%로 각각 전기영동적으로 균일하게 정제하였다. 두 효소의 분자량을 Sephadex G-200에 의해 측정한 결과, fruit-와 stem-bromelain의 분자량은 32.5 KDa와 37 KDa이었으며, 이들은 공히 1개의 subunit로 구성된 효소단백질이었다. Fruit-bromelain은 pH 8.0과 $70^{\circ}C$, stem-bromelain은 pH 7.0과 $60^{\circ}C$ 에서 각각 최대 활성을 나타내었다. 동 식물 유래의 천연단백질을 중심으로 기질 특이성을 검토한 결과 동물성 단백질보다는 밀단백 또는 ISP와 같은 식물성 단백질에 높은 기질 특이성을 보였으며, casein에 대한 fruit와 stem-bromelain의 또 $K_m$값은 0.18 mM와 0.34 mM이었다. pCMB는 경쟁적 저해제로서 fruit와 stem-bromelain의 $K_i$값은 0.18 mM과 0.10 mM이었다. pCMB에 의해 저하된 두 효소의 활성은 cysteine에 의해 재활성화 되었으며 또한 과량의 cysteine 첨가에 의해 활성화됨으로써 이 효소들은 전형적인 thiol계 단백분해효소임을 알 수 있었다.

• 한국물환경학회지
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• 제20권5호
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• pp.437-446
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• 2004

To develop sewage treatment apparatus by natural purification method, the sewage treatment plant that consisted of aerobic and anaerobic plot was constructed. And then, the effects of treatment conditions on the removal of pollutants in the relation to sewage loading, sewage injection method and season according to the pebble kind. Removal rate of BOD and COD according to the sewage loading in effluent were over 95 and 77%, respectively. Removal rate of nitrogen in treated water by aerobic plot and effluent using sand were about 22~40 and 49~75%, respectively. Those of effluent using sand 75%+ oyster shell 25% and sand 50%+ oyster shell 50% as filter media in comparison with using sand were about 7~25 and 16~23%, respectively. Removal rate of phosphorus in treated water by aerobic plot and effluent using sand as filter media were about 30~36 and 52~56%, respectively. Those of effluent using sand 75%+ oyster shell 25% and sand 50%+ oyster shell 50% in comparison with using sand as filter media were about 11~40 and 12~45%, respectively. Removal efficiency of BOD and COD according to the intermittent injection method of sewage were slightly decreased, but those of nitrogen and phosphorus were little varied in comparison with continuous injection method. Removal efficiency of BOD and COD in winter in comparison with the others were little varied, but those of nitrogen and phosphorus slightly decreased.

• 한국환경복원기술학회지
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• 제14권5호
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• pp.81-101
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• 2011

This study was conducted to understand the environmental and ecological function of habitat through evaluation of water environment, soil environment, vegetation characteristics, macro-invertebrate characteristics, and visual habits environment evaluation (SVAP) in Hongdong stream located in Hongseong-Gun, Chungnam Province, and hereafter to utilize the results for the habits reconstruction and improvement project. As the results of water quality analysis, BOD, COD, T-P was almost below the standard quality from upper stream to down stream. The construction of small reservoir, wetland and water purification facility, and the management of non-point pollution are proposed to improve these problems. The soil texture was sandy soil, which is unfitted with vegetation development. The construction of shallows and bogs, and induction of soil sedimentation and biotope formation are proposed to improve these problems. In the plant flora, total 90 kinds were observed with 81 species and nine varieties in total 36 families and 85 genera, and the naturalization rate was higher at down area than upper area. As the results of macro-invertebrate fauna survey, total 26 species and 297 individuals in 20 families and 22 genera were collected. Peltodytes sinensis, Chironomidae sp., and Culicidae sp., which are observed at polluted environment, were collected as dominant species. An appropriate vegetation management party idea is necessary, and it is done an idea in consideration of the soil and a physical characteristic. Visual habits environment evaluation (SVAP) result was mostly determined with below normal (Fair) grade. Pollution source interception, purification pond establishment, and various bog establishment are proposed to improve these problems. With the above results, the ecological environment was determined with bad condition, and the improvement of biotope was urgently needed through sustainable monitoring and management of streams habitat in rural area.

• Archives of Pharmacal Research
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• 제28권7호
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• pp.816-822
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• 2005

Catharsius protease-2 (CPM-2) was isolated from the body of dung beetles, Catharsius molossus, using a three step purification process (ammonium sulfate fractionation, gel filtration on Bio-Gel P-60, and affinity chromatography on DEAE Affi-Gel blue). The purified CPM-2, having a molecular weight of 24 kDa, was assessed homogeneously by SDS-polyacrylamide gel electrophoresis. The N-terminal amino acid sequence of CPM-2 was composed of X Val Gin Asp Phe Val Glu Glu lie Leu. CPM-2 was inactivated by $Cu^{2+}\;and\;Zn^{2+}$ and strongly inhibited by typical serine proteinase inhibitors such as TLCK, soybean trypsin inhibitor, aprotinin, benzamidine, and ${\alpha}_1$-antitrypsin. However, EDTA, EGTA, cysteine, $\beta$-mercaptoethanol, E64, and elastatinal had little effect on enzyme activity. In addition, antiplasmin and antithrombin III were not sensitive to CPM-2. Based on the results of a fibrinolytic activity test, CPM-2 readily cleaved $A{\alpha}-$ and $B{\beta}$-chains of fibrinogen and fibrin, and y-chain of fibrinogen more slowly. The nonspecific action of the enzyme resulted in extensive hydrolysis, releasing a variety of fibrinopeptides of fibrinogen and fibrin. Polyclonal antibodies of CPM-2 were reactive to the native form of antigen. The ELISA was applied to detect quantities, in nanograms, of the antigen in CPM-2 protein.

• 한국환경복원기술학회지
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• 제17권1호
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• pp.1-12
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• 2014

This study was carried out not only to identify the optimum concentrations of sulfur powder and citric acid treated for improving arsenic absorption of Pteris multifuda known as hyperaccumulator of arsenic, but also to develop arsenic purification model in the forest soil. After applying sulfur powder (0, 30, 45, $60g{\cdot}m^{-2}$) and citric acid (0, 200, 400, $800g{\cdot}m^{-2}$) in the forest soil contaminated with heavy metals, P. multifuda was planted and cultivated for 16 weeks. And then the growth and arsenic contents of plants were analyzed. In the result of research, the growth of P. mulifuda, except plant width, cultivated in soils treated with sulfur powder and citric acid was relatively lower than control. The accumulated amount of arsenic in aerial parts of P. multifuda ($1822.2mg{\cdot}kg^{-1}$) cultivated in soils treated with $200g{\cdot}m^{-2}$ citric acid was improved 62.5% against the control. And the accumulated amount of arsenic per 1 $m^2$ ($20.1mg{\cdot}m^{-2}$) was the greatest in $200g{\cdot}m^{-2}$ citric acid treatment. Translocation rate (TR) was higher in all acid treatment compare to control, and was the best in $200g{\cdot}m^{-2}$ citric acid treatment (0.95) especially. It showed that the arsenic absorbed in underground parts was transferred fast to aerial parts. Therefore, $200g{\cdot}m^{-2}$ citric acid treatment in the soil is recommended for arsenic purification using P. multifuda.