• Title/Summary/Keyword: Pure sciences

Search Result 782, Processing Time 0.022 seconds

Isolation of Antioxidant and Antibrowning Substance from Chionanthus retusa Leaves (이팝나무 잎으로부터 항산화 및 항갈변물질의 분리)

  • Lee, Young-Nam;Jeong, Chang-Ho;Shim, Ki-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.33 no.9
    • /
    • pp.1419-1425
    • /
    • 2004
  • This study was performed to examine in vitro antioxidative activities such as DPPH radical scavenging activity, reducing power and tyrosinase inhibitory effect of various solvent fractions from Chionanthus retusa leaves. Ethyl acetate fraction showed potent antioxidative activity and tyrosinase inhibitory effect. The active compound was isolated from the butanol fraction by silica gel column chromatography and MPLC. The isolated compound was luteolin-4'-O-glucoside determined by $^1H$, $^{13}C$-NMR and 2D NMR. Compared with several antioxidant compounds, luteolin-4'-O-glucoside exhibited effective DPPH radical scavenging activity and reducing power in a concentration dependent manner. Bioassay with pure luteolin-4'-O-glucoside showed a dose-independent inhibitory effect on L-DOPA oxidation by mushroom tyrosinase and its $IC_{50}$ values were established as 23.2 ${\mu}g/mL$. Therefore, we may suggest that luteolin-4'-O-glucoside can be used as a food additive possessing the potent antioxidative activity and skin-whitening cosmetic material.

Ectomycorrhizal Effect on Physiological Activities of Water-Stressed Nodulated Alnus rubra Seedlings (외생균근(外生菌根)이 수분결핍(水分缺乏)된 루브라 오리나무 묘목(苗木)의 생리활동(生理活動)에 미치는 영향(影響))

  • Koo, Chang-Duck;Molina, Randy;Miller, Steven L.
    • Journal of Korean Society of Forest Science
    • /
    • v.89 no.4
    • /
    • pp.513-521
    • /
    • 2000
  • Red alder (Alnus rubra Bong.) seedlings inoculated with Frankia pure cultures were grown in a walk-in growth chamber for sixteen weeks. Half were inoculated with the spores of the ectomycorrhizal fungus Alpova diplophloeus (Zeller & Dodge) Trappe & Smith. The mycorrhizal seedlings were significantly larger than nonmycorrhizal plants in diameter, and nodule and shoot dry weight by 6 to 16% when their heights were very similar. The mycorrhizal effects on water relations of red alder seedlings were explored in a 30 hours water stress. Mycorrhizal and nonmycorrhizal seedlings did not significantly differ in leaf water potentials, $CO_2$ exchange rates or $N_2$-fixation rates during the drought. Our results suggests that A. diplophloeus mycorrhizas increased red alder seedling growth under well-watered conditions but do not affect water relations of the plant under water-stress.

  • PDF

Determination of $SiO_2$ and $ZrO_2$ in Zircon Sand by Optical Emission Spectrometer (직독식 방출분광기를 이용한 지르콘사 중의 $SiO_2$$ZrO_2$의 분석)

  • Kim, Young Man;Jeong, Chan Yee;Han, Bong Han;Choi, Beom Suk
    • Analytical Science and Technology
    • /
    • v.6 no.3
    • /
    • pp.275-282
    • /
    • 1993
  • A direct and simultaneous method to determine the $SiO_2$ and $ZrO_2$ in zircon sand of raw mineral and its treated one were studied by optical emission spectrometer using DC arc source. The synthetic standard was prepared by mixing with pure metal oxide, and it was diluted with buffer(graphite) and flux($Li_2B_4O_7$). The mixing ratio of buffer and flux and its dilution ratio to sample was investigated in order to choose the best excitation conditions. The optimum mixing and dilution ratios were 0.22:1 and 40, and the standard deviations of analytical results were 1.9% for $SiO_2$ and 4.7% for $ZrO_2$.

  • PDF

Introgression of Sex-Limited Larval Markings to a Productive Multivoltine Strain of Silkworm Bombyx mori L.

  • Rao, D. Raghavendra;Singh Ravindra;Basavaraja H.K.;Kariappa B.K.;Dandin S.B.;Rufaie S.Z. Haque
    • International Journal of Industrial Entomology and Biomaterials
    • /
    • v.13 no.1
    • /
    • pp.7-14
    • /
    • 2006
  • A breeding programme was initiated during 2001 to introduce sex-limited larval markings to a productive multivoltine breed - BL67 from an inbred sex-limited line, MY1 (SL) maintained at Central Sericultural Research and Training Institute, Mysore. Introgressive hybridization, recurrent backcrossing for six generations followed by sib-mating resulted in synthesis of a new multivoltine silkworm breed BL67 (SL) with sex-limited larval markings. The new breed was studied for combining ability by crossing with eight bivoltine breeds viz., $NB_4D_2,\;CSR_2,\;CSR_2 (SL),\;CSR_2,\;CSR_4,\;CSR_8,\;CSR_{18}\;and\;CSR_{19}$. General combining ability effects of the new breed showed its superiority over the popular Pure Mysore by expressing significant GCA effects for six out of twelve characters whereas the results are on par with the original multivoltine breed. The hybrid $BL67(SL){\times}CSR2(SL)$ excelled in several quantitative characters such as pupation rate (90.2%), cocoon weight (1.97 g), cocoon shell weight (40 cg), cocoon shell ratio (20.3%), filament length (918 m), denier (2.96), raw silk percentage (14.96%) and neatness (90 p). Studies on cocoon size variability revealed that the cocoons of $BL67(SL){\times}CSR2(SL)$ were found comparatively uniform showing less standard deviation of 6.55 and co-efficient of variation of 3.91 %. The suitability of newly developed breed for easy grain age operation and commercial exploitation with promising hybrid have been discussed.

Evaluation of Mixtures of Certain Market Wastes as Silage

  • Ozkul, H.;Kilic, A.;Polat, M.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.24 no.9
    • /
    • pp.1243-1248
    • /
    • 2011
  • The aim of this study was to determine the usefulness of vegetable wastes as silage for ruminants. Varying amounts of wheat straw (WS), wheat bran (WB) and salt (S) were combined with minced vegetable wastes (VW) during ensilage. Seven different ingredient combinations were investigated viz: 100% VW (Group I, control), 90% VW+9% WS+1% S (Group II), 80% VW+15% WS+4% WB+1% S (Group III), 70% VW+20% WS+9% WB+1% S (Group IV), 90% VW+9% WB+1% S (Group V), 80% VW+15% WB+4% WS+1% S (Group VI) and 70% VW+20% WB+9% WS+1% S (Group VII). The inclusion of straw and bran increased (p<0.01) the DM content of silage. The highest contents of the pure silage were CP (p<0.001), EE (p<0.01) and NFE (p<0.05). NDF contents of VW silage and group V were significantly lower and especially the VW silage was found to have the lowest ADL content (p<0.01). The in vitro ME values of VW silage and bran added silage were higher than other groups (p<0.01). pH, lactic acid and acetic acid values of silage groups were changed between 4.09-4.20, 2.43-3.46% and 0.60-0.86%, respectively. In conclusion, different mixtures of VW have a high ensilage capacity and can serve as an alternative roughage source for ruminants. The addition of 9% bran significantly improved the silage in view of both dry matter content and nutritive value.

Inhibitory Activity of Nitric Oxide Synthase and Peroxynitrite Scavenging Activity of Extracts of Perilla frutescens (들깨 잎 추출물의 Nitric Oxide Synthase 저해활성 및 Peroxynitrite 소거활성)

  • Kim, Jae-Yeon;Kim, Ji-Sun;Jung, Chan-Sik;Jin, Chang-Bae;Ryu, Jae-Ha
    • Korean Journal of Pharmacognosy
    • /
    • v.38 no.2 s.149
    • /
    • pp.170-175
    • /
    • 2007
  • Activated microglia by neuronal injury or inflammatory stimulation overproduce nitric oxide (NO) by inducible nitric oxide synthase (iNOS) and reactive oxygen species (ROS) such as superoxide anion, resulting in neurodegenerative diseases. The toxic peroxynitrite (ONOO$^-$), the reaction product of NO and superoxide anion further contributes to oxidative neurotoxicity. We tried to evaluate the effects of two kinds of varieties of Perilla frutescens var japnica Hara on the NO production in lipopolysaccharide (LPS)-activated microglia. The perilla cultivars of Namcheondeulkkae (NC) and Boradeulkkae (BR) were developed by pure line from the local variety and by a cross between 'deulkkae' and 'chajogi', respectively. Spirit, hexane, chloroform and butanol fractions of the leaves of NC and BR inhibited the production of NO in LPS-activated microglia. The fractions of BR showed stronger activity than NC and the spirit extracts was the most potent in both cultivars. The solvent fractions of BR suppressed the expression of protein and mRNA of iNOS in LPS-activated microglial cells. Moreover, the extracts of NC and BR showed the activity of peroxynitrite scavenging in cell free bioassay system. These results imply that Namcheondeulkkae and Boradeulkkae might have neuroprotective activity through the inhibition of NO production by activated microglial cells and peroxynitrite scavenging activity.

The effects of a temporal masking on the sound laterlization (시간 마스킹이 음상정위에 미치는 영향)

  • Lee, Chai-Bong
    • The Journal of the Korea institute of electronic communication sciences
    • /
    • v.5 no.4
    • /
    • pp.352-356
    • /
    • 2010
  • In this study, it is discussed how the directional property of the sound lateralization is influenced by proceeding or succeeding tone. The acoustic source applied here is a reference sound which has 0.5 msec interaural time difference(ITD). Based on this reference sound, interfering sounds with five levels of magnitude are applied to the subjects with four kinds of inter-stimuli time intervals(ISI). The interfering sounds are also added as two different types, proceeding tone and succeeding tone. Additionally, in order to investigate a frequency influence, the reference sound and the interfering sounds are generated by using 2kHz, 4 kHz and a white noise. As a result, the influence on lateralization by proceeding tone is lager than that by succeeding tone. It can consider this result as the effect of temporal masking on lateralization. Moreover, there are small differences of masking effect on lateralization by combinations of pure tone. This result shows that the dependency of frequency domain between reference sound and interfering sound is small on the sound lateralization.

Crystallinity of CrOx/TiO2 Catalysts and Their Activity in TCE Oxidation (CrOx/TiO2 촉매의 결정성과 TCE 산화반응 활성)

  • Kim, Moon-Hyeon;Lee, Hyo-Sang
    • Journal of Environmental Science International
    • /
    • v.23 no.5
    • /
    • pp.829-837
    • /
    • 2014
  • Titania-supported chromium oxides with different loadings have been embarked in catalytic oxidation of trichloroethylene (TCE) to inquire association of the formation of crystalline $Cr_2O_3$ with catalytic performances. A better activity in the oxidative TCE decomposition at chosen temperatures was represented when chromium oxides ($CrO_x$) had been dispersed on pure anatase-type $TiO_2$ (DT51D) rather than on phase-mixed and sulfur-contained ones such as P25 and DT51. The extent of TCE oxidation at temperatures below $350^{\circ}C$ was a strong function of $CrO_x$ content in $CrO_x$/DT51D $TiO_2$, and a noticeable point was that the catalyst has two optimal $CrO_x$ loadings in which the lowest $T_{50}$ and $T_{90}$ values were measured for the TCE oxidation. This behavior in the activity with respect to $CrO_x$ amounts could be associated with the formation of crystalline $Cr_2O_3$ on the support surface, that is less active for the oxidation reaction, and an easier mobility of the surface oxygen existing in noncrystalline $CrO_x$ species with higher oxidation states, such as $Cr_2O_8$ and $CrO_3$.

Retrieval of Depolarization ratio using Sunphotometer data and Comparison with LIDAR Depolarization ratio (선포토미터 데이터를 이용한 편광소멸도 산출과 라이다 편광소멸도와의 비교)

  • Kim, Kwanchul;Choi, Sungchul;Noh, Youngmin
    • Korean Journal of Remote Sensing
    • /
    • v.32 no.2
    • /
    • pp.97-104
    • /
    • 2016
  • We present linear particle depolarization ratio at 440, 675, 870, and 1020 nm retrieved from measurements with an AERONET sun/sky radiometer at Osaka, Japan. The retrieved data were compared with lidar derived linear particle depolarization ratio at 532 nm at the same site. We find good agreement between linear particle depolarization ratios derived with Sun photometer and measured by lidar except for those at 440 nm. The coefficients of determination between lidar derived data and sun/sky radiometer derived data were 0.28, 0.81, 0.88, and 0.89 at 440, 675, 870, and 1020 nm, respectively. We find that the linear particle depolarization ratio derived with sun/sky radiometer varies by the mixing between Asian dust and pollution particles. As the mixing ratio of Asian dust and pollution particles is increased, the linear particle depolarization ratio values are lower than the values of pure Asian dust. It was confirmed by the value of single-scattering albedo and particle size distribution.

Enhancement of Dissolution Rate of Poorly Water-soluble Ibuprofen using Solid Dispersions and Inclusion Complex (고체분산체 및 포접화합물을 이용한 난용성 약물인 이부프로펜의 용출 속도의 증가)

  • Lee, Beom-Jin;Lee, Tae-Sub
    • Journal of Pharmaceutical Investigation
    • /
    • v.25 no.1
    • /
    • pp.31-36
    • /
    • 1995
  • Solid dispersions and inclusion complex were prepared for the enhancement of solubility and dissolution rate of poorly water-soluble ibuprofen(IPF) as a model drug. Polyethylene glycol 4000(PEG4000) and polyvinylpyrrolidone(PVP) were used for the preparation of solid dispersion. $2-Hydroxypropyl-{\beta}-cyclodextrin(2-HP{\beta}CD)$ was also used for the preparation of inclusion complex. The solubility of IPF increased as the concentration of PEG4000, PVP and $2-HP{\beta}CD$ increased. Solubilization capacity of $2-HP{\beta}CD$ was increased about 10 times when compared to PEG 4000 and PVP. The dissolution rate of drug from solid dispersions and inclusion complex in the simulated gastric fluid was enhanced when compared to pure IPF and commercial $BR4^{\circledR}$ tablet as a result of improvement of solubility. In case of solid dispersions, dissolution rate of drug was proportional to polymer concentration in the formulation. The marked enhancement of dissolution rate of drug by inclusion complexation with $2-HP{\beta}CD$ was noted. However, dissolution rate of drug from solid dispersions and inclusion complex in the simulated intestinal fluid was not significant because IPF was readily soluble in that condition. From these findings, water-soluble polymers and cyclodextrin were useful to improve solubility and dissolution rate of poorly water-soluble drugs. However, easiness and reliability of preparation method, scale-up and cost of raw materials must be considered for the practical application of solid dispersion and inclusion complex in pharmaceutical industry.

  • PDF