Browse > Article

Inhibitory Activity of Nitric Oxide Synthase and Peroxynitrite Scavenging Activity of Extracts of Perilla frutescens  

Kim, Jae-Yeon (College of Pharmacy, Sookmyung Women's University)
Kim, Ji-Sun (College of Pharmacy, Sookmyung Women's University)
Jung, Chan-Sik (Yeongnam Agricultural Research Institute, NICS, RDA)
Jin, Chang-Bae (Division of Life Sciences, Korea Institute of Science & Technology (KIST))
Ryu, Jae-Ha (College of Pharmacy, Sookmyung Women's University)
Publication Information
Korean Journal of Pharmacognosy / v.38, no.2, 2007 , pp. 170-175 More about this Journal
Abstract
Activated microglia by neuronal injury or inflammatory stimulation overproduce nitric oxide (NO) by inducible nitric oxide synthase (iNOS) and reactive oxygen species (ROS) such as superoxide anion, resulting in neurodegenerative diseases. The toxic peroxynitrite (ONOO$^-$), the reaction product of NO and superoxide anion further contributes to oxidative neurotoxicity. We tried to evaluate the effects of two kinds of varieties of Perilla frutescens var japnica Hara on the NO production in lipopolysaccharide (LPS)-activated microglia. The perilla cultivars of Namcheondeulkkae (NC) and Boradeulkkae (BR) were developed by pure line from the local variety and by a cross between 'deulkkae' and 'chajogi', respectively. Spirit, hexane, chloroform and butanol fractions of the leaves of NC and BR inhibited the production of NO in LPS-activated microglia. The fractions of BR showed stronger activity than NC and the spirit extracts was the most potent in both cultivars. The solvent fractions of BR suppressed the expression of protein and mRNA of iNOS in LPS-activated microglial cells. Moreover, the extracts of NC and BR showed the activity of peroxynitrite scavenging in cell free bioassay system. These results imply that Namcheondeulkkae and Boradeulkkae might have neuroprotective activity through the inhibition of NO production by activated microglial cells and peroxynitrite scavenging activity.
Keywords
Perilla frutescens; nitric oxide; peroxynitrite scavenging;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Lee, J. I., Han, E. D., Lee, S. T. and Park, H. W. (1986) Study on the evaluation of oil quality and the differences of fatty acid composition between varieties in perilla (Perilla frutescens Britton var. japonica Hara ). Korean J. Breeding 18:228-233
2 Park, H.-S., Ahn, B. and Yang, C.-B. (1990) Studies on the functional properties of sesame and perilla protein isolate. Korean J. Food Sci. Technol. 22: 350-356   과학기술학회마을
3 Kim, T. J. (1996) Natural plant resources in Korea, Seoul National University Press
4 Wolfe, T. A. and Dasta, J. F. (1995) Use of nitric oxide synthase inhibitors as a novel treatment for septic shock. Ann. Pharmacother. 29: 36-46   DOI
5 Kooy, N. W. and Royall, J. A., Ischiropoulos, H. and Beckman, J. S. (1994) Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radic. Biol. Med. 16: 149-156   DOI   ScienceOn
6 Wang, J. and Mazza, G. (2002) Inhibitory effects of anthocyanins and other phenolic compounds on nitric oxide production in LPS/IFN-gamma-activated RAW 264.7 macrophages. J. Agric. Food Chem. 50: 850-857   DOI   ScienceOn
7 Boissel, J. P., Schwarz, P. M. and Forstermann, U. (1998) Neuronal-type NO synthase: transcript diversity and expressional regulation. Nitric Oxide 2: 337-349   DOI   ScienceOn
8 Radi, R., Beckman, J. S., Bush, K. M. and Freeman, B. A. (1991) Peroxynitrite oxidation of sulfhydryls. The cytotoxic potential of superoxide and nitric oxide. J. Biol. Chem. 266: 4244-4250
9 Meda, L., Cassatella, M. A., Szendrei, G. I., Otvos, L. Jr., Baron, P., Villalba, M., Ferrari, D. and Rossi, F. (1995) Activation of microglial cells by beta-amyloid protein and interferon-gamma. Nature 374: 647-650   DOI   ScienceOn
10 Busse, R. and Fleming, I. (1995) Regulation and functional consequences of endothelial nitric oxide formation. Ann. Med. 27: 331-340   DOI
11 Green, L. C., Wagner, D. A., Glogowski, J., Skipper, P. L., Wishnok, J. S. and Tannenbaum, S. R. (1982) Analysis of nitrate, nitrite, and [$^{15}N$]nitrate in biological fluids. Anal. Biochem. 126: 131-138   DOI   ScienceOn
12 Moilanen, E. and Vapaatalo, H. (1995) Nitric oxide in inflammation and immune response. Ann. Med. 27: 359-367   DOI
13 Ignarro, L. J., Buga, G. M., Wood, K. S., Byrns, R. E. and Chaudhuri, G. (1987) Endothelium-derived relaxing factor produced and released from artery and vein is nitric oxide. Proc. Natl. Acad. Sci. U.S.A. 84: 9265-9269
14 Hibbs, J. B. Jr., Taintor, R. R., Vavrin, Z. and Rachlin, E. M. (1988) Nitric oxide: a cytotoxic activated macrophage effector molecule. Biochem. Biophys. Res. Commun. 157: 87-94   DOI   ScienceOn
15 Pfeilschifter, J., Eberhardt, W., Hummel, R., Kunz, D., Muhl, H., Nitsch, D., Pluss, C. and Walker, G. (1996) Therapeutic strategies for the inhibition of inducible nitric oxide synthasepotential for a novel class of anti-inflammatory agents. Cell Biol. Int. 20: 51-58   DOI   ScienceOn
16 Lee, K.-I., Rhee, S.-H., Kim, J.-O., Chung, H.-Y. and Park, K.-Y. (1993) Antimutagenic and antioxidative effects of perilla leaf extracts. J. Korean Soc. Food. Nutr. 22: 175-180   과학기술학회마을
17 McCartney-Francis, N., Allen, J. B., Mizel, D. E., Albina, J. E., Xie, Q. W., Nathan, C. F. and Wahl, S. M. (1993) Suppression of arthritis by an inhibitor of nitric oxide synthase. J. Exp. Med. 178: 749-754   DOI   ScienceOn
18 Sakai, T. and Hirose, Y. (1969) Farnesenes isolated from the volatile oil of Perilla frutescens f. viridis Makino. Bull. Chem. Soc. Jpn. 42: 3615   DOI   ScienceOn
19 Han, H.S., Park, J.H., Choe, H.J., Son, J.H., Kim, Y.H., Kim, S. and Choe, C. (2004) Biochemical analysis and physiological activity of perilla leaves. Korean J. Food Culture 19:94-105
20 Haenen, G. R., Paquay, J. B., Korthouwer, R. E. and Bast, A. (1997) Peroxynitrite scavenging by flavonoids. Biochem. Biophys. Res. Commun. 236: 591-593   DOI   ScienceOn
21 Salvemini, D., Misko, T. P., Masferrer, J. L., Seibert, K., Currie, M. G. and Needleman, P. (1993) Nitric oxide activates cyclooxygenase enzymes. Proc. Natl. Acad. Sci. U.S.A. 90:7240-7244
22 Yamazaki, M. and Saito, K. (2006) Isolation and characterization of anthocyanin 5-O-glucosyltransferase in Perilla frutescens var. crispa by differential display. Methods Mol. Biol. 317: 255-266
23 Park, K.-Y., Lee, K.-I. and Rhee, S.-H. (1992) Inhibitory effect of green-yellow vegetables on the mutagenicity in salmonella assay system and on the growth of AZ -521 human gastric cancer cells. J. Korean Soc. Food Nutr. 21: 149-153   과학기술학회마을
24 Gehrmann, J., Matsumoto, Y. and Kreutzberg, G. W. (1995) Microglia: intrinsic immuneffector cell of the brain. Brain Res. Brain Res. Rev. 20: 269-287   DOI   ScienceOn
25 Choung, M. G. (2005) Comparison of major characteristics between seed perilla and vegetable perilla. Korean J. Crop Sci. 50: 171-174   과학기술학회마을
26 Angus, J. A. and Cocks, T. M. (1989) Endothelium-derived relaxing factor. Pharmacol. Ther. 41: 303-352   DOI   ScienceOn
27 McGeer, P. L. and McGeer, E. G. (1995) The inflammatory response system of brain: implications for therapy of Alzheimer and other neurodegenerative diseases. Brain Res. Brain Res. Rev. 21: 195-218   DOI   ScienceOn
28 Szabo, C. (1996) The pathophysiological role of peroxynitrite in shock, inflammation, and ischemia-reperfusion injury. Shock 6: 79-88   DOI   ScienceOn
29 농작물 직무육성 신품종선정위원회 결과 보고서 (2001) 351-354, 농촌진흥청
30 Nagatsu, A., Tenmaru, K., Matsuura, H., Murakami, N., Kobayashi, T., Okuyama, H. and Sakakibara, J. (1995) Novel antioxidants from roasted perilla seed. Chem. Pharm. Bull. (Tokyo) 43: 887-889   DOI   ScienceOn