• Title/Summary/Keyword: Pure sciences

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Neuregulin-1 promotes cardiomyocyte differentiation of genetically engineered embryonic stem cell clones

  • Wang, Zhi;Xu, Guotong;Wu, Yalan;Liu, Shaowen;Sun, Baogui;Dai, Qiuyan
    • BMB Reports
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    • v.41 no.10
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    • pp.699-704
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    • 2008
  • Embryonic stem (ES) cell-derived cardiomyocytes (ESCMs) must be specifically purified in order to prevent teratoma formation, and this confusing issue has hampered their clinical application. We therefore investigated a technique to generate pure labeled ESCMs for possible use in cardiac repair. We generated transgenic ES cell lines expressing enhanced green fluorescent protein (EGFP) under the transcriptional control of the $\alpha$-cardiac myosin heavy chain ($\alpha$-MHC) promoter. Differentiated EGFP-positive ES cells displayed characteristics of CMs. Furthermore, neuregulin-1 (NRG-1) upregulated the expression of the cardiac-restricted transcription factors Nkx2.5 and GATA-4, as well as differentiated CM factors ($\alpha$-MHC, $\beta$-MHC). Immunohistochemistry demonstrated that NRG-1 increased expression of cardiac-specific troponin T in the beating foci of the embryoid bodies. This work revealed a potential method for specifically labeling and enriching ESCMs by combining genetically-engineered ES cell clones and exogenous growth factor treatment.

A Study on Self-Similarity in Turbulent Hydrogen Jet Flames with Coaxial Air (동축공기 수소확산 화염의 자기상사성에 대한 연구)

  • Kim, Mun-Ki;Kim, Seung-Han;Yoon, Young-Bin
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.30 no.5
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    • pp.71-78
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    • 2002
  • Experiments have been performed using two-color PIV in hydrogen non-premixed flames with various conditions of coaxial air, which was classified into three cases with/without reaction. Mean velocity, turbulence intensity and Reynolds stress were analyzed using flow fields from PIV measurement First, the similarity of pure jet had a good agreement with previous results of other researchers. It was found that the decay of centerline velocity was proportional to $x^{-1}$ in coaxial air conditions. By normalizing axial distance with effective jet diameter defined by effective density, the data of centerline velocity collapsed a single line. And the radial profiles of mean velocity showed that they didn't become self-similar because the curves differed from each other as coaxial air velocity increased at fixed fuel velocity. Also, turbulence intensity became self-similar further downstream than mean velocity.

Thin and superthin perforator flap elevation based on preoperative planning with ultrahigh-frequency ultrasound

  • Visconti, Giuseppe;Bianchi, Alessandro;Hayashi, Akitatsu;Cina, Alessandro;Maccauro, Giulio;Almadori, Giovanni;Salgarello, Marzia
    • Archives of Plastic Surgery
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    • v.47 no.4
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    • pp.365-370
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    • 2020
  • The ability to directly harvest thin and superthin perforator flaps without jeopardizing their vascularity depends on knowledge of the microsurgical vascular anatomy of each perforator within the subcutaneous tissue up to the dermis. In this paper, we report our experience with ultrahigh-frequency ultrasound (UHF-US) in the preoperative planning of thin and superthin flaps. Between May 2017 and September 2018, perforators of seven patients were preoperatively evaluated by both ultrasound (using an 18-MHz linear probe) and UHF-US (using 48- and 70-MHz linear probes). Thin flaps (two cases) and superthin flaps (five cases) were elevated for the reconstruction of head and neck oncologic defects and lower limb traumatic defects. The mean flap size was 6.5×15 cm (range, 5×8 to 7.5×23 cm). No complications occurred, and all flaps survived completely. In all cases, we found 100% agreement between the preoperative UHF-US results and the intraoperative findings. The final reconstructive outcomes were considered satisfactory by both the surgeon and the patients. In conclusion, UHF-US was found to be very useful in the preoperative planning of thin and superthin free flaps, as it allows precise anticipation of very superficial microvascular anatomy. UHF-US may represent the next frontier in thin, superthin, and pure skin perforator flap design.

Thermal and Creep Analysis of an Exhaust Duct of Smart UAV with FGM (경사기능재료를 사용한 스마트 무인기 덕트의 열해석과 크리프 해석)

  • Im, Jong-Bin;Park, Jeong-Seon;Yun, Dong-Yeong;Lee, Jeong-Jin
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.34 no.1
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    • pp.65-73
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    • 2006
  • The high temperature occurs due to the combustion gas from engine in unmanned aerial vehicles (UAV). The high temperature may cause serious damages in UAV structure. The Functionally Graded Material (FGM) is chosen as a candidate material of the engine duct structure. A functionally graded material (FGM) is a two- component mixture composed by compositional gradient materials from one material to the other. In contrast, traditional composite materials are homogeneous mixtures, and involve compositions between the desirable properties of the component materials. Since significant proportions of an FGM contain the pure form of each material, the need for compromise is eliminated. The properties of both components can be fully utilized. Thermal stress analysis of FGM layers (20, 40, 60, 80 and 100) is performed in this paper. In addition, the creep behavior of FGM applied in duct structure of an engine is analyzed for better understanding of FGM characteristics.

Biosorption Model and Factors for Removing Lead to Aureobasdium pullulans being Imperfect Fungus (불완전 균류 Aureobasdium pullulans으로 납을 제거하기 위한 인자들과 흡착모델)

  • Suh, Jung-Ho;Suh, Myung-Gyo;Chung, Kyung-Tae;Lee, Yong-Hee
    • Journal of Life Science
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    • v.16 no.6
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    • pp.877-883
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    • 2006
  • An alternative method to remove and recover heavy metals is biosorption based on metal-sequestering properties of natural or biological origin. In this study, the effects of factors such as temperature, pH, initial concentration of lead, and initial amount of biomass on biosorption of lead using Aureobasdium pullulans were investigated. A. pullulans has an excellent selectivity to remove lead than other heavy metals such as cadmium, chromium, nickel in pure and mixed solution. The optimum temperature of biosorption with A. pullulans was $40^{\circ}C$ and the amount of removal increased at high pH. The higher initial lead concentration or the lower cell dry weight, the higher amount of lead was adsorbed. The adsorption isotherm of lead was accorded with Freundlich model. The adsorption capacity and initial adsorption rate of living A. pullulans were about twice higher than that of dead one.

Effect of Operating Conditions and Recovery of Water Back-washing in Spiral Wound Microfiltration Module Manufactured with PVDF Nanofibers for Water Treatment (수처리용 PVDF 나노섬유 나권형 정밀여과 모듈에서 운전조건의 영향과 물 역세척 회복)

  • Kyung, Kyu Myung;Park, Jin Yong
    • Membrane Journal
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    • v.25 no.2
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    • pp.180-190
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    • 2015
  • PVDF (polyvinylidene fluoride) nanofiber has the advantages such as excellent strength, chemical resistance, nontoxic, non-combustibility. Flat membranes with 0.3 and $0.4{\mu}m$ pore size respectively, were manufactured by PVDF nanofiber, and then each spiral wound module was prepared with them. A woven paper was not included in preparing the module with $0.3{\mu}m$ pore size; however, it was included the module with $0.4{\mu}m$ pore size. The permeate fluxes and rejection rates of the two modules were compared using pure water and simulation solution including kaolin and humic acid. The recovery rate and filtration resistance were calculated after water back-washing. In addition, the effect of flow rate and trans-membrane pressure on treatment efficiency and filtration resistance were investigated for the spiral wound module with $0.4{\mu}m$ pore size.

A Minerlogical Study of Plagioclase in Volcanic Rocks from the Mt. Mudeung Area (무등산지역 화산암류에서 산출되는 사장석의 광물학적 연구)

  • Park Byung-Kyu;Kim Yong-Jun;Kim Youn-Joong
    • Journal of the Mineralogical Society of Korea
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    • v.18 no.3 s.45
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    • pp.155-164
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    • 2005
  • Volcanic rocks from the Mt. Mudeung area which are composed of Hwasun andesite, Mudeungsan dacite and Togok rhyolite contain plagioclase phenocrysts in common. Majority of the repeated twins observed in optical microscopy are albite twin and some are pericline twin. EPMA studies of plagioclases from Hwansun andesite, Mudeungsan dacite and Togok rhyolte indicate calcic andesine, andesine-oligoclase, nearly pure albite, respectively Albite twin and pericline twin can be easily distinguished through TEM diffraction patterns, which is quite difficult by optical microscopy. Plagioclases in volcanic rocks from the Mt. Mudeung area do not show e-reflection in (100) electron diffraction patterns, probably because of their high cooling rate, which inhibited phase separations during cooling.

Self-Adaptation framework for TCP Selection (TCP 선택을 위한 자동 적응 프레임워크)

  • Hwang, Jae-Hyun;Yoo, Chuck
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.34 no.2B
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    • pp.130-142
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    • 2009
  • In this paper, we propose a self-adaptation framework that selects a TCP variant adapted to current end-to-end path among available TCP variants. There is no single version of TCP that is suitable to all network environments since the causes for performance degradation are different one another according to characteristics of network environments. Thus, determining that which TCP variants should be selected in order to get best performance is very important. To enable adaptation through such determination, we integrate the existing network estimation schemes and some TCP variants into our framework then make light-weight performance knowledge database for TCP selection. Through implementing and evaluating the proposed framework we show that our solution can help TCP get high and stable performance on the various types of network environments by pure end-to-end.

Growth and Electrochemical Behavior of Poly[Ni(saldMp)] on Carbon Nanotubes as Potential Supercapacitor Materials

  • Zhang, Yakun;Li, Jianling;Kang, Feiyu;Wang, Xindong;Ye, Feng;Yang, Jun
    • Bulletin of the Korean Chemical Society
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    • v.33 no.6
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    • pp.1972-1978
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    • 2012
  • The polymer of (2,2-dimethyl-1,3-propanediaminebis(salicylideneaminato))-nickel(II), Ni(saldMp), was deposited on multi-walled carbon nanotubes (MWCNTs) substrate by the route of potential linear sweep. The nano structures of poly[Ni(saldMp)] have been obtained by adjusting the monomer concentration of 0.1, 0.2, 0.5, 1.0 and 1.5 mmol $L^{-1}$. The poly[Ni(saldMp)] prepared in acetonitrile solution with monomer concentration of 1.0 mmol $L^{-1}$ shows the fastest growth rate. The effects of potential window on charge-discharge efficiency and electrodeposition scan number on capacitance performance were discussed. Poly[Ni(saldMp)] prepared with less electrodeposition scans exhibits higher capacitance, but this goes against the improvement of the whole electrode capacitance. Sample with 8 deposition scans is the best compromise with the geometric specific capacitance 3.53 times as high as that of pure MWCNTs, and 1.24 times for the gravimetric specific capacitance under the test potential window 0.0-1.0 V.

Hearing Ability of Redlip croaker Pseudosciaena polyactis cultured in the Coastal Sea of Jeju (제주 연안에서 양식된 참조기의 청각 능력)

  • AHN, Jang-Young;KIM, Seok-Jong;CHOI, Chan-Moon;PARK, Young-Seok;LEE, Chang-Heon
    • Journal of Fisheries and Marine Sciences Education
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    • v.28 no.2
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    • pp.384-390
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    • 2016
  • The purpose of this paper is to improve the availability of underwater sound by the fundamental data on the hearing ability of Redlip croaker Pseudosciaena polyactis, which is cultured according to the cultivation technology, recently. The auditory thresholds of Redlip croaker were determined at 6 frequencies from 80Hz to 800Hz by heartbeat conditioning method using pure tones coupled with a delayed electric shock. The audible range of the Redlip croaker extended from 80Hz to 800Hz with the best sensitive frequency range including little difference in hearing ability from 80Hz to 500Hz. In addition, the auditory thresholds over 800Hz increased rapidly. The mean auditory thresholds of the Redlip croaker at the test frequencies from 80Hz to 800Hz were 90.7dB, 93.4dB, 92.9dB, 94.4dB, 95.5dB and 108dB, respectively. Auditory masking for the redlip croaker was measured using masking stimuli with the spectrum level range of about 66, 71, 75dB (0dB re $1{\mu}Pa/{\sqrt{Hz}}$). According to white noise level, the auditory thresholds increased as compared with thresholds in a quiet background noise. The Auditory masking by the white noise spectrum level was stared over about 70dB within 80~500Hz. Critical ratio ranged from minimum 20.7dB to maximum 25.5dB at test frequencies of 80Hz~500Hz.