• The Korean Journal of Zoology
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• v.11 no.4
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• pp.103-117
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• 1968

Insect hemocytes during metamorphosis were studies by histchemical and autoradiographic methods is Pieris rapae (Lepidoptera). The hemocytes were classified into six types, prohemocytes, plasmatocytes, podocytes, granular hemocytes, spherule cells and oenocytoids on the basis of the transitions in shapes and inclnsions of cytoplasms. Proteins, nucleic acids, polysaccharides and lipids in the hemocytes were detected histochemically from larval to pupal stages to learn the rise and fall of them during growth and metamorphosis. Most of the granules consisted of glycogen, neutral mucopolysaccharides and mucoprotein in addition to some granules of neutral fats and phospholipids were found in the granular hemocytes and spherule cells. Mitotic figures and DNA synthetic activities were observed in every type of hemocytes from 2nd to 5th instars, suggesting the all types of hemocytes originated from the prohemocytes. The cytoplasmic filaments of plasmatocytes and pdocytes extended very long in prepupa and pupa and the vermiform cells were the transformed plasmatocytes due to their further differentiation.

• Korean journal of applied entomology
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• v.23 no.2 s.59
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• pp.132-136
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• 1984

This study was conducted to investigate the bionomics of Curculio sikkimensis in Chonnam province from 1981 to 1983. Damage rates of the chestunt were $26.4\%$ in Damyang, $21.1\%$ in Gwangyang, and $12.9\%$ in Seungju. Adults occurred from the mid-July to the mid-September and showed peak from the mid-August to the early-September. Larvae escaped the chestnut from the early-October to the early-November. Overwintering larvae emerged $3.3\%$ in the first year, $22.78\%$ in the second year and continously overwintered $1.67\%$ in the third year. Larvae showed the highest distribution at $1.67\%$ of soil depth during the overwintering. Mortality of overwintering larvae and course of time showed the linearly correlation. Period of each stage was 5.9 days for egg and 17.17 days for pupa. Adult longevity was 9.10 days for female and 7.03 days for male.

• The Korean Journal of Mycology
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• v.1 no.2
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• pp.25-30
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• 1973

The Aspergilli can be isolated from many of damaged domestic fowl's lung which were collected at fowl slaughter house from July to August 1973, during two months at Dapsimri, Dongdaemoon-ku, Seoul city. The survey of incubation condition for carbon source and nitrogen source were summerized as follows: 1. One of the Aspergillius fumigatus group were isolated from damaged domestic fowl's lung. 2. In the preparation of isolation media, adding the 4 gs of silkworm pupa and Meju to Czapek's agar medium is more preferable than Austick's media (2% malt extract agar) 3. In survey of incubation condition, the growth rate and sporulation shown that higher differences according to varieties and amounts of C-source. And in the case of 20% C-source concentration shown that low growth rate than lower concentration of C-source. 4. In N-source, the growth rate indicated that some difference according to varieties of N-source, difference of growth rate demonstrated almost not shown at various amounts differences of mycelium and sporulation was only detected reversely.

• Applied Biological Chemistry
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• v.31 no.2
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• pp.143-146
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• 1988

The effect of gamma irradiation on controlling infestation of insects on rice grain were investigated. The egg and larva stages of rice weevil (Sitophilus oryzae) were more radiosensitive than the pupal and adult stages. The complete mortal dose of the first two stages was 0.05kGy, while it was 0.5kGy in the second two stages. In indian-meal moth (plodia interpuctella Hubner), sterile doses of egg, larva, pupa and adult were 0.04 to 0.05, 0.05 to 0.06 and 0.2 to 0.25kGy, respectively. Therefore, the irradiation dose within 1.0kGy was considered to be effective for rice storage so far as reinfestation was prevented. Attractiveness of indian-meal moth toward the rice irradiated at 1.0 to 5.0kGy was non-significant in F-test.

• Journal of Wetlands Research
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• v.17 no.2
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• pp.113-117
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• 2015

Even though many researches are conducted for the conservation and restoration of endangered species Menyanthes trifoliata, recently, there is no study on the threatening factors to this plant. This is the first time in Korea to study growth and feeding characteristics of Deilephila elpenor as a threatening factor to Menyanthes trifoliata through an experiment. Experiment was done with 6 Eephant hawk-moth larvae and change of body weight, food preference, and ingestion amount of Bog-bean were investigated. It took 27 days from larva to pupa and maximum body weight of lavae was in the range of 4-7.5g. The food preference sequence of the lavae was Menyanthes trifoliata, Impatiens balsamina, Ampelopsis brevipedunculata var. heterophylla, Parthenocissus tricuspidata. Ingestion model shows the total amount of ingestion by a larva is 11-30g and this amount can be acquired at $0.03-0.08m^2$ of Menyanthes trifoliata pure stand. This study showed Deilephila elpenor as a potential threatening factor and suggests that the conservation and restoration plan of endangered species Menyanthes trifoliata include the control plan of Deilephila elpenor, also.

• Animal Bioscience
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• v.37 no.8
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• pp.1440-1451
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• 2024

Objective: This study aimed to develop and evaluate the effectiveness of a water-soluble microencapsulation method for probiotic strains using gum Arabic (GA) and skim milk (SKM) over a three-month storage period following processing. Methods: Four strains of Pediococcus acidilactici (BYF26, BYF20, BF9, and BF14) that were typical lactic acid bacteria (LAB) isolated from the chicken gut were mixed with different ratios of GA and SKM as coating agents before spray drying at an inlet temperature 140℃. After processing, the survivability and probiotic qualities of the strains were assessed from two weeks to three months of storage at varied temperatures, and de-encapsulation was performed to confirm the soluble properties. Finally, the antibacterial activity of the probiotics was assessed under simulated gastrointestinal conditions. Results: As shown by scanning electron microscopy, spray-drying produced a spherical, white-yellow powder. The encapsulation efficacy (percent) was greatest for a coating containing a combination of 30% gum Arabic: 30% skim milk (w/v) (GA:SKM30) compared to lower concentrations of the two ingredients (p<0.05). Coating with GA:SKM30 (w/v) significantly enhanced (p<0.05) BYF26 survival under simulated gastrointestinal conditions (pH 2.5 to 3) and maintained higher survival rates compared to non-encapsulated cells under an artificial intestinal juices condition of pH 6. De-encapsulation tests indicated that the encapsulated powder dissolved in water while keeping viable cell counts within the effective range of 10⁶ for 6 hours. In addition, following three months storage at 4℃, microencapsulation of BYF26 in GA:SKM30 maintained both the number of viable cells (p<0.05) and the preparation's antibacterial efficacy against pathogenic bacteria, specifically strains of Salmonella. Conclusion: Our prototype water-soluble probiotic microencapsulation GA:SKM30 effectively maintains LAB characteristics and survival rates, demonstrating its potential for use in preserving probiotic strains that can be used in chickens and potentially in other livestock.

• Food Science of Animal Resources
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• v.44 no.4
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• pp.790-804
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• 2024

This study compared the physicochemical properties of edible insect oils from silkworm (Bombyx mori) pupa (SP), sago palm weevil (Rhynchophorus ferrugineus) larva (PW), and bamboo caterpillar (Omphisa fuscidentalis; BC) to oils from chicken skin (CK), beef back fat (BF), pork back fat (PF), salmon belly (SB), sea bass belly (BB), coconut (C), and peanut (P). The fatty acid profiles and thermal behaviors (crystallization and melting) of the extracted oils were evaluated. PW and BC oils had more saturated fatty acids (SFAs) than CK, PF, SB, BB, and P oils. SP oil had equivalent SFA content to CK and BB oils. Insect oils exhibited similar monounsaturated fatty acid concentrations in all samples, except C oils. PW and BC oils exhibited a higher content of palmitoleic acid than the other oils. SP oils contained polyunsaturated fatty acids similar to those in SB and BB oils, which were higher than those in PW, BC, CK, BF, and PF oils. SP oil also exhibited the highest concentration of α-linolenic acid (C18:3 n-3). Arachidonic acid (0.01-0.02 g/100 g) in all insect oils was lower level compared to CK, BF, PF, SB, and BB oils. SP oil (0.03 g/100 g) exhibited a slightly higher level of eicosapentaenoic acid compared to PW (0.01 g/100 g) and BC (0.01 g/100 g) oils. The insect oils were liquid at ambient temperature, solid below -15℃, and required less energy (∆H_m-max) for melting than other samples. This study indicated that insects, particularly SP, could serve as an alternative source of fat to meet its growing demand.

• Korean journal of applied entomology
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• v.50 no.2
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• pp.157-165
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• 2011

Enterobacteria were isolated in the gut of the predacious multicolored Asian ladybird beetle, Harmonia axyridis, and their effects to the development of H. axyridis were examined. Populations of H. axyridis in this experiment were collected from Kimjae at Cheonbuk province (JK population), Geumsan at Chungnam province (CK population) and laboratory population at Laboratory of Insect Physiology in Chungnam National University, Daejeon. Thirty-four enterobacteria isolates were purified and isolated from the digestive tract of H. axyridis, and a total of 4 strains were classified into group by analysis of 16S rRNA gene sequences. About 70% of total isolates were phylogenetic groups of Bacillus genus and Staphylococcus genus, and they were commonly separated from the digestive tract of H. axyridis. After investigating their susceptibility against antibiotics with 18 representative enterobacteria isolates, ofloxacin and penicillin were selected for examination in this study of their ability to inhibit the growth of all of isolates. In order to remove the enterobacteria from the aphids, ofloxacin and penicillin were given to the green peach aphid, Myzus persicae, and the turnip aphid, Lipaphis erysimi. These aphids were provided to H. axyridis as prey. The weight of pupa, developmental periods of each larval instar, the number of eggs and their hatching ratio of H. axyridis with treatment aphids were lower compared with non-treatment aphids. Staphylococcus saprophyticus is a representative enterobacteria and commonly isolated from the digestive tract of H. axyridis. In the absence of S. saprophyticus, the developmental periods of each larval instar increased; however, the weights of pupa, the number of eggs, and their hatching ratio decreased.

• Korean Journal of Environmental Biology
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• v.21 no.1
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• pp.72-76
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• 2003

We investigated the effects of molting-hormone insecticide tebufenozide on fourth larvae of the midge C. flaviplumuon in growth development from fourth-instar to adult. Fourth-instar larvae were exposed test concentrations were chosen control, 10 $\mu\textrm{g}$$L^{-1}$, 30 $\mu\textrm{g}$$L^{-1}$, 60 $\mu\textrm{g}$$L^{-1}$ and 100$\mu\textrm{g}$$L^{-1}$. In control and 10 $\mu\textrm{g}$$L^{-1}$, 10~20% individuals succeed adults while low emergence rates were observed surplus 30 $\mu\textrm{g}$$L^{-1}$. Death larvae appeared a various day along the concentration. We observed the converged day: control, 10 $\mu\textrm{g}$$L^{-1}$, 30 $\mu\textrm{g}$$L^{-1}$ and 60~100 $\mu\textrm{g}$$L^{-1}$ were day 4 to day 18, day 13 to day 16, day 9 to day 17 and day 9 to day 15, respectively. Therefore we concerned a tendency that exposure concentration was high, death period was short. Also, due to molting hormone disruption, development of midge was postponed relatively low concentration such as 10 $\mu\textrm{g}$$L^{-1}$. The converged days of pupa stage revealed control, 10 $\mu\textrm{g}$$L^{-1}$, 30 $\mu\textrm{g}$$L^{-1}$ and 60~100$\mu\textrm{g}$$L^{-1}$ were day 11~15, day 16~20, day 9~15 and day 5~9, respectively. About 10% individuals grow up the pupa-stage in 60~100 $\mu\textrm{g}$$L^{-1}$ condition. Sex ratio of the emerged adults was not different in this study. Death pupae frequently appeared in day 13~16 in control, day 6~23 in 10 $\mu\textrm{g}$$L^{-1}$, day 13~16 in 30 $\mu\textrm{g}$$L^{-1}$ and day 6~16 in 60~100 $\mu\textrm{g}$$L^{-1}$.

• Korean journal of applied entomology
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• v.58 no.4
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• pp.355-362
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• 2019

Cotesia glomerata L., an internal parasitoid wasp, attacks the larvae of both the cabbage white butterfly (Artogeia rapae L.) and the diamondback moth (Plutella xylostella L.). It can be utilized as a natural biological enemy to control these two insect pests in the summer cabbage fields of the Korean highland areas. The developmental response and sex ratio of C. glomerata to various temperatures and its longevity were examined in the laboratory. The egg-to-larva and pupa stages of C. glomerata were 12.1 ± 2.1 and 6.4 ± 1.8 days, respectively, at 20℃, The developmental threshold for egg-to-larva and pupa stages were 7.7 and 8.5℃, respectively. The sex ratios of C. glomerata when reared under various temperatures were 61.0 ± 4.5% at 15℃, 44.2 ± 1.0% at 20℃, and 39.0 ± 2.3% at 25℃, and the incidence of females increased as the temperature decreased. The longevity of C. glomerata when fed a 10% sugar solution was 20.4 ± 0.2 days, while in adults without any feed, the longevity was 3.6 ± 0.1 days. Indoor reared C. glomerata adults were released into cabbage fields from 2007 to 2018, in early August of each year, and the outdoor parasitism rates were surveyed. The parasitism rates were found to increase gradually as the year passed (Y = 0.2696X + 2.8633, R² = 0.3994). The highest parasitism rate was observed in 2013 at 7.6%, and the lowest was in 2018 at 6.5 %. These results could be used as basic information for biological control of kimchi cabbage pests at highland fields.