• ALGAE
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• 제37권2호
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• pp.149-161
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• 2022

Noctiluca scintillans is a heterotrophic dinoflagellate that causes red-colored oceans during the day (red tides) and glowing oceans at night (bioluminescence). This species feeds on diverse prey, including phytoplankton, heterotrophic protists, and eggs of metazoans. Thus, many scientists have conducted studies on the ecophysiology of this species. It is easy to cultivate N. scintillans at a scale of <1 L, but it is difficult to cultivate them at a scale of >100 L because N. scintillans cells usually stay near the surface, while prey cells stay below the surface in large water tanks. To obtain mass-cultured N. scintillans cells, we developed an automatic system for cultivating N. scintillans on a scale of 100 L. The system consisted of four tanks containing fresh nutrients, the chlorophyte Dunaliella salina as prey, N. scintillans for growth, and N. scintillans for storage, respectively. The light intensities supporting the high growth rates of D. salina and N. scintillans were 300 and 20 µmol photons m^-2 s^-1, respectively. Twenty liters of D. salina culture from the prey culture tank were transferred to the predator culture tank, and subsequently 20 L of nutrients from the nutrient tank were transferred to the prey culture tank every 2 d. When the volume of N. scintillans in the predator culture tank reached 90 L 6 d later, 70 L of the culture were transferred to the predator storage tank. To prevent N. scintillans cells from being separated from D. salina cells in the predator culture tank, the culture was mixed using an air pump, a sparger, and a stirrer. The highest abundance of N. scintillans in the predator culture tank was 45 cells mL^-1, which was more than twice the highest abundance when this dinoflagellate was cultivated manually. This automatic system supplies 100 L of N. scintillans pure culture with a high density every 10 d for diverse experiments on N. scintillans.

• 항공우주기술
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• 제4권2호
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• pp.142-152
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• 2005

액체로켓 엔진시스템 개발에 있어서 시동부터 정상상태까지의 시동특성은 안정된 시동의 재현성을 확보하여 신뢰도를 높이는 것과 과도상태의 시간을 단축하는 두 가지 목표를 만족하는 방향으로 전개된다고 할 수 있다. 특히 우리나라와 같이 액체로켓 엔진 개발의 초기단계에서는 엔진시스템 시험의 경험이 없어, 시동에서 정상상태까지 과연 어느 정도의 시간이 필요할 것인가에 대한 예측이 힘들 수밖에 없다. 본 연구에서는 터보펌프 공급식 액체로켓 엔진의 각 구성품의 모델을 구성하여 시동 과도 해석을 수행할 수 있는 프로그램을 완성하였다. 이 프로그램을 이용하여 25톤급 가스발생기 사이클 엔진에 대한 시동 특성을 조사하여 시동에서 정상상태에 이르는 시간을 계산하였으며, 비정상 상태의 엔진시스템의 동특성을 밝힐 수 있었다.

• 청정기술
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• 제7권4호
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• pp.273-280
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• 2001

흡수기는 흡수식 열펌프 시스템에서 중요한 구성요소일 뿐만 아니라 흡수기의 성능은 전체 시스템에 중요한 영향을 미친다. 본 연구에서는 기포분사형 흡수기에서의 암모니아기체의 흡수열의 효과적 제거를 위한 냉각수 방향으로의 열전달에 대해 실험적 연구를 수행하였다. 흡수기에 유입되는 암모니아 기체의 유속, 암모니아 수용액의 유속, 농도, 온도, 흡수기의 지름, 높이, 기체와 용액의 유입 방향등 여러 가지 변수에 대하여 열전달 성능의 특성을 살펴본 결과, 기체의 주입량, 용액의 주입량 증가는 열전달 성능 향상에 기여하며, 용액의 온도나 농도의 상승은 열전달 성능에 방해요소로 작용하였으며 흐름방향이 향류인 경우 열전달 성능에 향상이 있었다. 본 실험의 데이터를 이용하여 상관관계식을 유도하여 열전달에 대한 복잡한 관계를 일반화 하였다.

• 한국산학기술학회논문지
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• 제14권7호
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• pp.3164-3169
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• 2013

본 연구에서는 수직형 지중 열교환기의 3차원 CFD 해석 기법을 제안하여 2개소의 현장 열응답 시험과 비교하였다. CFD 해석 비교를 위해 GAMBIT을 이용하여 지중과 지중 열교환기 형상을 모델링하였으며, 상용코드인 FLUENT를 사용하여 3차원 열전달 유동 해석을 수행하였다. 2개소의 현장 열응답 시험에서 도출된 지중 유효 열전도도와 지중 초기온도를 경계조건으로 사용하였으며, 시간에 따른 지중 열교환기의 입구온도 변화는 profile을 사용하여 실제 조건을 모사하였다. CFD 해석 결과 2개소의 지중 열교환기 출구온도는 $0.5^{\circ}C$ 범위 내에서 예측하였고, 기울기는 1.6% 이내에서 적절히 예측하였다. 향후 CFD 해석 기법을 활용하여 지중 열교환기 깊이, 형상 및 배열 변화 등에 따른 성능예측에 활용하고자 한다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권10호
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• pp.1460-1468
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• 2003

An experiment was conducted to quantify the flow of soluble non-ammonia nitrogen (SNAN) in the liquid phase of ruminal (RD) and omasal digesta (OD), and to investigate diurnal pattern in SNAN flow in OD. Five ruminally cannulated Finnish-Ayrshire dairy cows in a $5{\times}5$ Latin square design consumed a basal diet of grass silage and barley grain, and that supplemented with four protein feeds (kg/d DM basis) as follows: skimmed milk powder (2.1), wet distiller' solubles (3.0), untreated rapeseed meal (2.1) and treated rapeseed meal (2.1). Ruminal digesta was sampled using a vacuum pump, whereas OD was collected using an omasal sampling system at 1.0 h interval during a 12 h feeding cycle. Both RD and OD were acidified, centrifuged to remove microbes and precipitated with trichloroacetic acid followed by centrifugation. The SNAN fractions (free amino acid (AA), peptide and soluble protein) in RD and OD were assessed using ninhydrin assay. Free AA, peptide and soluble protein averaged 60.0, 89.4 and 2.1 g/d, respectively, for RD, and 81.8, 121.5 and 2.5 g/d, respectively, for OD. Although free AA flow was relatively high, mean peptide flow was quantitatively the most important fraction of SNAN, indicating that degradation of peptide to AA rather than hydrolysis of soluble protein to peptide or deamination may be the most limiting step in rumen proteolysis. Diurnal pattern in flow of peptide including free AA in OD during a 12 h feeding cycle peaked 1 h post-feeding, decreased by 3 h post-feeding and was relatively constant thereafter. Protein supplementation showed higher flow of peptide including free AA immediately after feeding compared with no supplemented diet. There were no differences among protein supplements in diurnal pattern in flow of peptide including free AA in OD.

• Asian-Australasian Journal of Animal Sciences
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• 제25권9호
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• pp.1269-1275
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• 2012

An experiment was conducted to study the effect of soluble protein supplements on concentration of soluble non-ammonia nitrogen (SNAN) in the liquid phase of ruminal (RD) and omasal digesta (OD) of Korean native steers, and to investigate diurnal pattern in SNAN concentration in RD and OD. Three ruminally cannulated Korean native steers in a $3{\times}3$ Latin square design consumed a basal diet of rice straw and corn-based concentrate (control), and that supplemented (kg/d DM basis) with intact casein (0.24; IC) or acid hydrolyzed casein (0.46; AHC). Ruminal digesta was sampled using a vacuum pump, whereas OD was collected using an omasal sampling system at 2.0 h intervals after a morning feeding. The SNAN fractions (free amino acid (AA), peptide and soluble protein) in RD and OD were assessed using the ninhydrin assay. Concentrations of free AA and total SNAN in RD were significantly (p<0.05) lower than those in OD. Although free AA concentration was relatively high, mean peptide was quantitatively the most important fraction of total SNAN in both RD and OD, indicating that degradation of peptide to AA rather than hydrolysis of soluble protein to peptide or deamination may be the most limiting step in rumen proteolysis of Korean native steers. Diurnal variation in peptide concentration in OD for the soluble protein supplemented diets during the feeding cycle peaked 2 h post-feeding and decreased thereafter whereas that for the control was relatively constant during the entire feeding cycle. Diurnal variation in peptide concentration was rather similar between RD and OD.

• 한국추진공학회지
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• 제8권3호
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• pp.37-43
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• 2004

증기발생기 슬러지 세정을 위한 고압분사 실험의 분류궤적에 대한 가시화 및 속도분포를 측정함으로써 최적의 노즐형상을 찾기 위하여 플랜져방식의 고압펌프(사용압력 : 200 kg/$\textrm{cm}^2$, 15HP, 11kW. 토출유량 Q : 301/min)를 이용하였다. 가시화를 통하여 정성적인 분포를 비교분석할 수 있었고, 분류의 속도분포등을 통하여 노즐 형상비에 따른 유동의 차이를 2차원 PDPA 광학 계측장비를 사용하여 분석하였다. 고압 분사시 형상비에 관계없이 모든 경우에 있어서 중심축을 따라 모든 궤적들은 거의 유사함을 보였으나, 분류 중심에서 벗어난 외곽영역에서는 많은 차이가 있음을 알 수 있었다 이는 형상 비에 따른 주변 공기와의 마찰 및 분류 유입현상 때문이라 여겨진다.

• 한국전자파학회논문지
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• 제22권10호
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• pp.927-933
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• 2011

본 논문에서는 전자 소자 기반의 상용 오실로스코프에 의한 기존의 펄스 신호 측정 방법의 주파수 한계를 극복하기 위하여 전기광학 기반의 측정 방법을 기술하였다. 펄스폭 0.1 ps의 펨토초 레이저와 광다이오드를 사용하여 20 GHz 주파수 범위에 대응되는 전자기 펄스를 발생시키고, 마이크로스트립 선로를 통해 전송되는 전자기 펄스를 검출하기 위하여 전기광학 샘플링 기법을 이용하였다. 마이크로스트립 선로 위의 매우 근접한 거리에서 광학 결정 프로브를 이용하여 비접촉식으로 펄스 신호를 검출하고, 선로의 출력단 펄스 신호를 기존의 오실로스코프로 측정하여 두 측정 결과를 서로 비교하였다.

• 한국환경보건학회지
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• 제44권3호
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• pp.205-216
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• 2018

Objectives: Scientific verification for health effects has been constantly demanded through the forest healing factors. In this study, phytoncide concentration which is one of the forest healing factors, was investigated according to stand density, season and visiting time, and analyzed correlation with micrometeorology factors. Methods: Total volatile organic compounds (TVOCs) and Natural volatile organic compounds (NVOCs) were collected using a measuring instrument which is connected to an air pump with the Tenax TA tube. The 32NVOCs were selected through the detailed criteria of adequacy assessment for recreational forest. The statistical analysis (correlation and stepwise regression analysis) was conducted between phytoncide concentration and micrometeorology factors. Results: NVOCs concentration linearly increased according to stand density. The high level showed in the summer (p<0.05), and there is no significant difference according to visiting hours of the Healing forest. NVOCs is a negative correlation with solar radiation, PAR and wind direction, and a positive correlation with relative humidity and temperature (p<0.01). NVOCs increased following the increase of humidity and temperature ($R^2=0.55$). Conclusions: Phytoncide linearly increased according to stand density, and showed the correlation significantly with microclimate factors. In future, these results will be utilized as a basic material to promote the generation of phytoncide, which positively influences human health promotion and manage the forest welfare space.

• Journal of Advanced Marine Engineering and Technology
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• 제31권3호
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• pp.263-271
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• 2007

The heat transfer coefficient and pressure drop during 9as cooling process of $CO_2$ (R744) in a helically coiled copper tube with the inner diameter of 4.55 mm and outer diameter of 6.35 mm were investigated experimentally. The main components of the refrigerant loop are a receiver, a variable-speed pump. a mass flow meter a pre-heater and a helically coiled type gas cooler (test section). The refrigerant mass fluxes are varied from 200 to $800kg/m^2s$ and the inlet pressures of gas cooler are 7.5 to 10.0 MPa. The heat transfer coefficients of $CO_2$ in a helically coiled tube are higher than those in a horizontal tube. The Pressure drop of $CO_2$ in the gas cooler shows a relatively good agreement with those predicted by Ito's correlation developed for single-phase in a helically coiled tube. The local heat transfer coefficient of $CO_2$ agrees well with the correlation by Pitla et al. However. at the region near pseudo-critical temperature. the experiments indicate higher values than the Pitla et al correlation. Therefore, various experiments in helically coiled tubes have to be conducted and it is necessary to develop the reliable and accurate prediction determining the heat transfer and pressure drop of $CO_2$ in a helically coiled tube.