• Title/Summary/Keyword: Pulsed

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Effects of Inoculum Level and Pressure Pulse on the Inactivation of Clostridium sporogenes Spores by Pressure-Assisted Thermal Processing

  • Ahn, Ju-Hee;Balasubramaniam, V.M.
    • Journal of Microbiology and Biotechnology
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    • v.17 no.4
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    • pp.616-623
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    • 2007
  • The effects of initial concentration and pulsed pressurization on the inactivation of Clostridium sporogenes spores suspended in deionized water were determined during thermal processing $(TP;\;105^{\circ}C,\;0.1MPa)$ and pressure-assisted thermal processing $(PATP;\;105^{\circ}C\;and\;700MPa)$ treatments for 40 min and 5min holding times, respectively. Different inoculum levels $(10^4,\;10^6\;and\;10^8CFU/ml)$ of C. sporogenes spores suspended in deionized water were treated at $105^{\circ}C$ under 700MPa with single, double, and triple pulses. Thermally treated samples served as control. No statistical significances (p>0.05) were observed among all different inoculum levels during the thermal treatment, whereas the inactivation rates $(k_1\;and\;k_2)$ were decreased with increasing the initial concentrations of C. sporogenes spores during the PATP treatments. Double- and triple-pulsed pressurization reduced more effectively the number of C. sporogenes spores than single-pulse pressurization. The study shows that the spore clumps formed during the PATP may lead to an increase in pressure-thermal resistance, and multiple-pulsed pressurization can be more effective in inactivating bacterial spores. The results provide an interesting insight on the spore inactivation mechanisms with regard to inoculum level and pulsed pressurization.

Effect of current waveform on drop transfer in pulsed gas metal arc welding (Pulsed GMAW 의 전류 파형이 금속이행에 미치는 영향)

  • Hammad, Muhammad A.;Yoo, Choong-D.
    • Proceedings of the KWS Conference
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    • 2009.11a
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    • pp.48-48
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    • 2009
  • Conventionally in pulsed gas metal arc welding (GMAW-P), drop transfer is analyzed with simplest square pulse waveform. While the pulse current is described by four parameters (peak current magnitude and time plus base current magnitude and time), it deviates the real pulse shape. Real pulse can be better idealized by the trapezoidal pulse waveform described by two additional parameters, i.e., current rise and fall rate (dI/dt). Power source response rate is described by these parameters. In this work, the effect of these parameters on drop transfer is predicted by the force displacement model (FDM). While peak current has significant effects on drop detachment, drop transfer is also influenced by the current rise rate. Predictions indicate that the current rise rate can have considerable effects on the size of the detached drop if other pulse parameters are kept constant. FDM is applied to determine peak time for one drop one pulse condition (ODOP) when rests of the pulse parameters are given. The predicted range of ODOP shows good agreement with experimental data.

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The Effect of Wound Healing of Pulsed Ultrasound and Chitosan in Diabetic Induced Model (당뇨유발 모델에서 맥동성초음파와 키토산의 창상치유효과)

  • Kim Gye-Yeop;Min Soon-Gyu;Cheong Mee-Sun
    • The Journal of Korean Physical Therapy
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    • v.16 no.3
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    • pp.50-64
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    • 2004
  • The studies have been designed to evaluate the effect of wound healing of pulsed ultrasound and chitosan application in diabetic incisive wound of rats. Mild diabetes mellitus was induced in rat used 30 mg/kg streptozotocin. Full thickness skin incision was made on the backs of Sprague-Dawley rats. We used 72 Sprague-Dawley rats which were divided into 4 groups; the subjects were divided into group of 6 rats each 3, 6, and 15 days. The results were summarized as follows; The rate of wound length of pulsed ultrasound with chitosan application groups more decreased than only pulsed ultrasound treatment group. The density of inflammatory cells in the experimental groups was more significantly decreased than diabetic control group(p<0.05). Historically, in the ultrasound with chitosan application groups, reepithelized epithelium was thicker and the collagen fiber were organized in a liner manner and connective tissue was matured faster those of the diabetic control group(p<0.05). From the conclusions above, in this study application of pulsed ultrasound and chitosan can be an effective way of promotion of wound healing in diabetic model.

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Heat input effects on microstructure quenched and tempered steel ASTM A517 to stainless steel AISI 316L

  • Pezeshkian, Rouhollah Mohsen;Shafaiepour, Saiedeh
    • Journal of Welding and Joining
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    • v.33 no.1
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    • pp.41-48
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    • 2015
  • In this study, the effect of heat input on weld metal microstructure and the effects of dissimilar weld heat affected zone in quenched and tempered ASTM A517 on the stainless steel AISI 316L is investigated through the optimization of welding parameters. For this purpose, two welding techniques are used, tungsten-conventional gas and pulsed gas with weld wire ER 309MoL with Diameter 2.4 mm. Research showed that the grain size of the heat affected zone in pulsed welding is less compared with conventional welding; weld metal structure is fully austenitic, it has a finer structure in the pulsed method. Additionally, the growth of weld metal adjacent steel A517 is different from steel 316L. Further, investigation showed that the rate of dilution is less in the pulsed method and the impact energy is increased in each three regions of the weld metal and heat affected zones in the pulsed method; the fracture in the weld metal and heat affected zone of steel 316L is quite soft and it is semi-crispy in the heat affected zone of steel A517.

Magnetic Pulsed Compaction and Sintering Characteristics of Al Composite Powders Reinforced with Waste Stainless Steel Short Fibers (폐 스테인레스강 단섬유로 강화한 알루미늄 복합분말의 자기펄스압 성형 및 소결 특성)

  • Hyun, Chang-Yong;Won, Chul-Hyun;Park, Jae-Soon
    • Journal of Powder Materials
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    • v.14 no.6
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    • pp.380-385
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    • 2007
  • Characteristics of Al-based composites with waste stainless steel short fiber, fabricated by magnetic pulsed compaction and sintering were investigated. The compacts prepared by magnetic pulsed compaction showed high relative density and homogeneous microstructure compared with that by conventional press compaction. The relative density of sintered composites at $430^{\circ}C$ for 1 h exhibited the same value with compacts and decreased with increase in STS short fiber content. The reaction between Al and STS phase was confirmed by the microstructural analysis using EDS. The sintered composites, prepared by magnetic pulsed compaction, showed increased hardness value with increasing STS fiber content. Maximum yield strength of 100 MPa and tensile strength of 232 MPa were registered in the AI-based composite with 30 vol% STS short fiber.

Pulsed-laser-diode Intermittently Pumped 2-㎛ Acousto-optic Q-switched Tm:LuAG Laser

  • Wen, Ya;Jiang, Yan;Zheng, Hao;Zhang, Hongliang;Wang, Chao;Wu, Chunting;Jin, Guangyong
    • Current Optics and Photonics
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    • v.4 no.3
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    • pp.238-246
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    • 2020
  • The heat distribution in crystals in a 2-㎛ acousto-optic Q-switched Tm:LuAG laser pumped by pulsed-laser-diode (pulsed-LD) intermittent-pumping technology was analyzed using COMSOL software. The thermal lensing effect of the Tm:LuAG crystal can be mitigated by pulsed-LD intermittent-pumping techniques. An experimental setup using this kind of approach achieved maximum output energy of 8.31 mJ, minimum pulse width of 101.9 ns, and highest peak power of 81.55 kW, reached at a Q-switched repetition rate of 200 Hz. It offers significant improvement of performance of the output laser beam, compared to pulsed-LD double-ended pumping technology at the same repetition rate.

A Comparative Study of CrN Coatings Deposited by DC and Pulsed DC Asymmetric Bipolar Sputtering for a Polymer Electrolyte Membrane Fuel Cell (PEMFC) Metallic Bipolar Plate (DC 스퍼터법과 비대칭 양극성 펄스 스퍼터법으로 제작된 고분자 전해질 연료전지 금속분리판용 CrN 코팅막의 특성 연구)

  • Park, Sang-Won;Chun, Sung-Yong
    • Journal of the Korean Ceramic Society
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    • v.50 no.6
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    • pp.390-395
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    • 2013
  • Nanocrystalline CrN films were deposited on Si (100) substrates by means of asymmetric pulsed DC reactive magnetron sputtering. We investigated the growth behavior, corrosion resistance and mechanical properties of CrN films with a change in the duty cycle and pulse frequency. The grain size of the CrN films decreased from 25.4 nm to 11.2 nm upon a decrease in the duty cycle. The corrosion potentials for the CrN films by DC sputtering was approximately - 0.6 V, and it increased to - 0.3 V in the CrN films which underwent pulsed sputtering. The nanoindentation hardness of the CrN films also increased with a decrease in the duty cycle. This enhancement of the corrosion resistance and mechanical properties of pulsed sputtered CrN films could be attributed to the densification and surface smoothness of the microstructure of the films.

The Effects of Pulsed Elctromagnetic Fields on Expression of Neurotrophic Factors after Spinal Cord Hemisection in Rats

  • Kang, Ji-Hyuk;Park, Sang-Young;Lee, Yun-Seob
    • Journal of Magnetics
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    • v.16 no.3
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    • pp.253-258
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    • 2011
  • The purpose of this study was to identify the effect of pulsed electromagnetic fields on the expression of neurotrophic factors after spinal cord injury. Sprague-Dawley male rats were given a spinal cord hemisection and randomly divided into 2 groups, the control and experimental groups. The experimental group was administered a fifteen minutes session of pulsed electromagnetic field once a day, five days a week. In order to observe the effect of these pulsed electromagnetic fields, this study observed the BDNF expression in the rat's lumbar spinal cord and the H&E staining in the gastrocnemius at 3, 7, 14, 21 days group after spinal cord hemisection. The results of this showed that the immunoreactivity of the BDNF in the rat's spinal cord gradually increased in each group. At 21 days, there is a significant difference between the control and experimental groups. The morphological shape of the gastrocnemius was gradually changed from 3days to 21days, and the gastrocnemius at 21 days was significantly degraded. However, the experimental group showed a slightly more organized gastrocnemius than the control group at 21days. The Results of this study suggest that pulsed electromagnetic field application decreases the degeneration of a rat's gastrocnemius morphology, and increases the immunoreactivity of the BDNF in the rat's spinal cord after spinal cord hemisection.

The Microbe Removing Characteristics Caused by Dirty Water Using a Simple Pulsed Power System

  • Kim, Hee-je;Song, Keun-ju;Song, Woo-Jung;Kim, Su-Weon;Park, Jin--Young;Joung, Jong-Han
    • KIEE International Transactions on Electrophysics and Applications
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    • v.4C no.3
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    • pp.91-95
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    • 2004
  • The pulsed power system is widely available for use in pulse generator applications. Generally, the pulse generator is required for very short pulse width and high peak value. We have designed and fabricated our own pulsed type power system and through its use, we investigated microbe removal characteristics. This paper introduces a simple pulsed power system for removing various microbes caused by dirty water. This system includes a 2 times power supply circuit, IR2110 operated by using a fixed voltage regulator 7812 and 7805, and the switching MOSFET (Metal Oxide Semiconductor Field Effect Transistor). We can also control this process by using a PIC one chip microprocessor. As a result, we can obtain good removing characteristics of various microbes by adjusting the charging voltage, the pulse repetition rate and the electrical field inducing time.

Pulse energy high Power test of metal film resistor (메탈 필름 저항의 펄스 대전력 시험)

  • Son, Y.G.;Jang, S.D.;Kwon, S.J.;Oh, J.S.;Cho, M.H.;Lee, K.T.
    • Proceedings of the KIEE Conference
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    • 2005.07c
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    • pp.2103-2105
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    • 2005
  • Metal film type of resistor have been tested to invest maximum usable power at the pulsed high voltage and pulsed high current. Experiments were carried out using capacitor charging power supply and dump switch. Pulsed amplitude were varied from 1kV to 25kV. The peak current reached was 1kA. Datasheet are given for the limited pulsed power and energy for metal film type of resistor in nanosecond and microsecond time range. The experimental investigation of the threshold loading of the resistor in the high current and voltage pulsed mode has shown that the process of destruction of resistor has specific features for each mode. The mechanisms of failure and destruction of resistors under action of high-voltage and high-current pulses are discussed.

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