• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.2
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• pp.128-135
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• 2005

Mixed-mode hydrophobic/ionic matrices exhibit a salt-tolerant property for adsorbing target protein from high-ionic strength feedstock, which allows the application of undiluted feedstock via an expanded bed process. In the present work, a new type of mixed-mode adsorbent designed for expanded bed adsorption, Fastline $PRO^{\circledR}$, was challenged for the capture of nattokinase from the high ionic fermentation broth of Bacillus subtilis. Two important factors, pH and ion concentration, were investigated with regard to the performance of nattokinase ad-sorption. Under initial fermentation broth conditions (pH 6.6 and conductivity of 10 mS/cm) the adsorption capacity of nattokinase with Fastline PRO was high, with a maximum capacity of 5,350 U/mL adsorbent. The elution behaviors were investigated using packed bed adsorption experiments, which demonstrated that the effective desorption of nattokinase could be achieved by effecting a pH of 9.5. The biomass pulse response experiments were carried out in order to evaluate the biomass/adsorbent interactions between Bacillus subtilis cells and Fastline PRO, and to demonstrate a stable expanded bed in the feedstock containing Bacillus subtilis cells. Finally, an EBA process, utilizing mixed-mode Fastline PRO adsorbent, was optimized to capture nattokinase directly from the fermentation broth. The purification factor reached 12.3, thereby demonstrating the advantages of the mixed-mode EBA in enzyme separation.

• Korean Journal of Optics and Photonics
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• v.32 no.2
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• pp.62-67
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• 2021

We have experimentally demonstrated sum-frequency generation (SFG) in a periodically poled lithium niobate (PPLN) crystal, using a mode-locked picosecond-pulsed fiber laser and a continuous-wave (CW) diode laser with a narrow linewidth. The mode-locked fiber laser had a center wavelength of 1560.7 nm and a spectral width of 1.1 nm, and the CW diode laser had a center wavelength of 1551.0 nm and a spectral width of 6 MHz. To effectively realize SFG, both of the spatial modes of the two lasers were made to overlap in the PPLN crystal by using a single-mode optical fiber. The pulse-mode SFG with pulsed- and CW-mode lasers was successfully observed in the spectral and time domains. These results are expected to be applicable in various ways, such as optical frequency measurement and high-resolution laser spectroscopy studies using optical frequency combs.

• Journal of the Korean Magnetics Society
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• v.13 no.3
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• pp.115-120
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• 2003

We have developed laser micro-machining technology for fabrication of the micro thin-film inductors. After the thin layers of FM/M/FM films were coated to the silicon substrate by using the conventional sputtering method, the new laser machining was applied to the patterning process that used to be carried out by the semiconductor lithography procedure. A CW Nd:YAG laser operating in TEM$\sub$00/ mode was actively Q-switched to obtain the very short pulse of 200 ns. The laser micro-machining process with pulse energy and repetition rate have been optimized as 5 mJ/pulse and 5 kHz, respectively, to obtain the line resolution as fine as 20 $\mu\textrm{m}$.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.46 no.12
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• pp.14-21
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• 2009

In this paper, a Ka-band 10 W power amplifier module with seven power MMIC bare dies is designed and fabricated using MIC technology which combines multiple MMIC chips on a thin film substrate. Modified Wilkinson power dividers/combiners and CBFGCPW-Microstrip transitions for suppressing resonance and reducing connection loss are utilized for high-gain and high-power millimeter wave modules. A new TTL pulse timing control scheme is proposed to improve output power degradation due to large bypass capacitors in the gate bias circuit. Pulse-mode operation time is extended more than 200 nsec and output power increase of 0.62 W is achieved by applying the proposed scheme to the Ka-band 10 W power amplifier module operating in the pulsed condition of 10 kHz and $5\;{\mu}sec$. The implemented power amplifier module shows a power gain of 59.5 dB and an output power of 11.89 W.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.300.2-300.2
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• 2014

테라헤르쯔(terahertz : THz)파는 0.1~10 THz 의 범위로 적외선과 방송파 사이에 광대역 주파수 스펙트럼을 차지하고 있으며 직진성, 투과성, 그리고 낮은 에너지(meV)를 가지고 있어 비 파괴적이고 무해한 장점을 지니고 있다. Ti:sapphire laser와 같은 femto-pulse source 등이 많은 발전이 되어 현재 많은 연구와 발전이 이루어지고 있다. femto-pulse source를 이용한 THz 응용에서는 높은 저항, 큰 전자이동도, 그리고 아주 짧은 전하수명의 기판을 요구하는데 저온에서 성장한(low-temperature grown : LT) InGaAs는 격자 내에 Gallium 자리에 Arsenic이 치환 하면서 AsGa antisite가 발생하여 전하수명을 짧아지는 것을 응용하여 가장 많이 이용되고 있다. 본 연구에서는 보다 높은 저항을 얻기 위하여 molecular beam epitaxy를 이용하여 semi-insulating InP:Fe 기판위에 격자 정합된 LT-InGaAs:Be/InAlAs multi quantum well (MQW)를 well과 barrier를 가각 $10{\mu}m$ 씩 100주기 성장을 하였고 Ti와 Au를 각각 30, $200{\mu}m$로 dipole antenna를 제작 하였다. 이 때 Ti:sapphire femto-pulse laser (30 fs/90 MHz)를 excitation source로 사용하였을 때 9000 pA로 LT-InGaAs epilayer (180 pA)보다 50배 이상 큰 전류 신호를 얻을 수 있었다. THz 발생과 검출을 초소형, 초경량, 고효율로 하기 위해서는 fiber-optic를 이용해야 하는데 이때 분산과 산란 손실이 가장 적은 1550 nm 대역에서 많은 연구가 이루어 졌다. 780, 1560 nm의 mode-locking laser (90 fs/100 MHz)를 사용하여 현재 많이 이용되고 있는 Ti:sapphire femto-pulse laser와 비교하여 THz 특성 변화를 확인하는 연구를 진행 하고 있다.

• The Journal of the Acoustical Society of Korea
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• v.38 no.1
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• pp.106-113
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• 2019

In this paper, we propose an algorithm to classify the received active pulse when the active sonar system is operated as a non-cooperative mode. The proposed algorithm uses CNN (Convolutional Neural Networks) which shows good performance in various fields. As an input of CNN, time frequency analysis data which performs STFT (Short Time Fourier Transform) of the received signal is used. The CNN used in this paper consists of two convolution and pulling layers. We designed a database based neural network and a pulse feature based neural network according to the output layer design. To verify the performance of the algorithm, the data of 3110 CW (Continuous Wave) pulses and LFM (Linear Frequency Modulated) pulses received from the actual ocean were processed to construct training data and test data. As a result of simulation, the database based neural network showed 99.9 % accuracy and the feature based neural network showed about 96 % accuracy when allowing 2 pixel error.

• Medical Lasers
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• v.8 no.1
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• pp.24-27
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• 2019

Seborrheic keratoses (SKs) have been treated with non-ablative longpulsed (LP) lasers, including LP 532-nm neodymium (Nd): yttrium aluminum garnet (YAG), LP 695-nm ruby, LP 755-nm alexandrite (Alex), and LP 1,064-nm Nd:YAG lasers, with a pulse durations of 1-300 msec. Dual-wavelength LP 755-nm Alex/1,064-nm Nd:YAG laser systems have been used to remove hair follicles and treat various vascular and pigmented disorders by sequentially delivering two pulses of different wavelengths with interpulse intervals in the millisecond range. This paper reports the case of a female patient with multiple, discrete, light brown SKs on the dorsum of both hands that were treated effectively with one session of dual-wavelength LP 1,064-nm Nd:YAG/755-nm Alex laser treatment. The treatment settings for the LP Nd:YAG laser were comprised of a wavelength of 1,064 nm, fluence of 50 J/cm², pulse duration of 5 msec, and beam size of 3 mm. The settings for the LP Alex laser were comprised of a wavelength of 755 nm, fluence of 50 J/cm², pulse duration of 5 msec, and beam size of 3 mm. A hybrid mode was used to automatically deliver LP Nd:YAG and LP Alex laser pulses in succession at interpulse intervals of 20 msec. Six weeks after treatment, the patient exhibited remarkable improvement of the light brown seborrheic keratoses and was satisfied with the results.

• Restorative Dentistry and Endodontics
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• v.27 no.2
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• pp.158-174
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• 2002

The aim of this study was to investigate the effect of light irradiation modes on polymerization shrinkage, degree of cure and microleakage of a composite resin. VIP$^{TM}$ (Bisco Dental Products, Schaumburg, IL, USA) and Optilux 501$^{TM}$ (Demetron/Kerr, Danbury, CT, USA) were used for curing Filtek$^{TM}$ Z-250 (3M Dental Products, St. Paul., MN, USA) composite resin using following irradiation modes: VIP$^{TM}$ (Bisco) 200mW/$\textrm{cm}^2$ (V2), 400mW/$\textrm{cm}^2$ (V4), 600mW/$\textrm{cm}^2$ (V6), Pulse-delay (200 mW/$\textrm{cm}^2$ 3 seconds, 5 minutes wait, 600mW/$\textrm{cm}^2$ 30seconds, VPD) and Optilux 501$^{TM}$ (Demetron/Kerr) C-mode (OC), R-mode (OR). Linear polymerization shrinkage of the composite specimens were measured using Linometer (R&B, Daejeon, Korea) for 90 seconds for V2, V4, V6, OC, OR groups and for up to 363 seconds for VPD group (n=10, each). Degree of conversion was measured using FTIR spectrometer (IFS 120 HR, Bruker Karlsruhe, Germany) at the bottom surface of 2 mm thick composite specimens V2, Y4, V6, OC groups were measured separately at five irradiation times (5, 10, 20, 40, 60 seconds) and OR, VPD groups were measured in the above mentioned irradiation modes (n=5 each). Microhardness was measured using Digital microhardness tester (FM7, Future-Tech Co., Tokyo, Japan) at the top and bottom surfaces of 2mm thick composite specimens after exposure to the same irradiation modes as the test of degree of conversion(n=3, each). For the microleakage test, class V cavities were prepared on the distal surface of the ninety extracted human third molars. The cavities were restored with one of the following irradiation modes : V2/60 seconds, V4/40 seconds, V6/30 seconds, VPD , OC and OR. Microleakage was assessed by dye penetration along enamel and dentin margins of cavities. Mean polymerization shrinkage, mean degree of conversion and mean microhardness values for all groups at each time were analyzed using one-way ANOVA and Duncan's multiple range test, and using chi-square test far microleakage values. The results were as follows : . Polymerization shrinkage was increased with higher light intensity in groups using VIP$^{TM}$ (Bisco) : the highest with 600mW/$\textrm{cm}^2$, followed by Pulse-delay, 400mW/$\textrm{cm}^2$ and 200mW/$\textrm{cm}^2$ groups, The degree of polymerization shrinkage was higher with Continuous mode than with Ramp mode in groups using Optilux 501$^{TM}$ (Demetron/Kerr). . Degree of conversion and microhardness values were higher with higher light intensity. The final degree of conversion was in the range of 44.7 to 54.98% and the final microhardness value in the range of 34.10 to 56.30. . Microleakage was greater in dentin margin than in enamel margin. Higher light intensity showed more microleakage in dentin margin in groups using VIP$^{TM}$ (Bisco). The microleakage was the lowest with Continuous mode in enamel margin and with Ramp mode in dentin margin when Optilux 501$^{TM}$ (Demetron/Kerr) was used.

• Journal of IKEEE
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• v.25 no.3
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• pp.485-492
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• 2021

In this paper, we propose the development of a defect inspection system for polygonal containers. Embedded board consists of main part, communication part, input/output part, etc. The main unit is a main arithmetic unit, and the operating system that drives the embedded board is ported to control input/output for external communication, sensors and control. The input/output unit converts the electrical signals of the sensors installed in the field into digital and transmits them to the main module and plays the role of controlling the external stepper motor. The communication unit performs a role of setting an image capturing camera trigger and driving setting of the control device. The input/output unit converts the electrical signals of the control switches and sensors into digital and transmits them to the main module. In the input circuit for receiving the pulse input related to the operation mode, etc., a photocoupler is designed for each input port in order to minimize the interference of external noise. In order to objectively evaluate the accuracy of the development of the proposed polygonal container defect inspection system, comparison with other machine vision inspection systems is required, but it is impossible because there is currently no machine vision inspection system for polygonal containers. Therefore, by measuring the operation timing with an oscilloscope, it was confirmed that waveforms such as Test Time, One Angle Pulse Value, One Pulse Time, Camera Trigger Pulse, and BLU brightness control were accurately output.

• Bulletin of the Korean Space Science Society
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• 2008.10a
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• pp.30.3-30.3
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• 2008

In this study, we investigate the magnetic latitude of phase reversal on the sudden commencement (SC)-associated preliminary impulse with 267 SC events using the ground magnetometer data of the IMAGE from 1997 to 2005. During SC event, geomagnetic fields are affected by various currents flowing in the magnetosphere and/or ionosphere. In particular, high-latitude geomagnetic field variations are significantly dominated by the change of SC-associated field aligned current (FAC). Until now, however, there are few studies to examine where the location of the FAC in the ionosphere is and what determines the location of the FAC. The location of the SC-associated FAC can be examined by using magnetometer data obtained from high-latitude stations distributed along the same magnetic meridian. The phase reversal locations are concentrated two regions, ~62 deg (L~4.5) and ~70 deg (L~8.5) in magnetic latitude. If FAC is a result of a mode conversion from fast mode to Alfven mode, then the FAC location could be determine by the duration time of the input energy. When we use the rise time, dT, as the input energy, there is no relationship between dT and the location where the first pulse of SC is reversed. We consider other factors such as local time and solar wind condition.