• Proceedings of the KSME Conference
• /
• 2000.04b
• /
• pp.535-540
• /
• 2000

The objective of the present study is to visualize the pulsatile flow fields by using three-dimensional computer simulation and the PIV system. A closed flow loop system was built for the steady and unsteady experiments. The Harvard pulsatile pump was used to generate the pulsatile pressure and velocity waveforms. Conifer powder as the tracing particles was added to water to visualize the flow field. Two consecutive particle images were captured by a CCD camera for the image processing. The cross-correlation method in combination with the moving searching area algorithm was applied for the image processing of the flow visualization. The pulsatile flow fields were visualized effectively by the PIV system in conjunction with the applied algorithm. The range validation and the area interpolation methods were used to obtain the final velocity vectors with high accuracy. The finite volume predictions were used to analyze three-dimensional flow patterns in the bifurcation model. The results of the PIV experiment and the computer simulation are in good agreement and the results show the recirculation zones and formation of the paired secondary flow distal to the apex of the bifurcated model. The results also show that the branch flow is pushed strongly to the inner wall due to the inertial force effect and helical motions are generated as the flow proceeds toward the outer wall.

• Proceedings of the KSME Conference
• /
• 2003.11a
• /
• pp.765-768
• /
• 2003

The objective of the present study was to visualize the pulsatile flow field in a branching model by using the high-resolution PIV system. A bifurcated flow system was built for the experiments in the pulsatile flow. Harvard pulsatile pump was used to generate the pulsatile velocity waveforms. Conifer powder as the tracing particles was added to water to visualize the flow fields. Two consecutive particle images at several cross sections of the flow filed were captured by the CCD cameras ($1K{\ast}1K$ and $640{\ast}480$). The results after the image processing clearly showed the recirculation zones and the formation of the paired secondary flows from the distal to the apex in the bifurcated model. The results also indicated that the flow velocities in the inner wall moved faster than those in the outer wall due to the inertial force effects and the helical motions generated in the branch flows as the flow proceeded toward the outer wall. While the PIV images from the $1K{\ast}1K$ camera were closer to the simulation results thantheimagesfromthe640${\ast}$480camera,bothresultsofthePIVexperimentsusingthetwocamerasgenerallyagreed quitewellwiththeresultsfromthenumericalsimulation.

• The Korean Journal of Pharmacology
• /
• v.23 no.2
• /
• pp.57-75
• /
• 1987

Two modalities of gonadotropin secretion, pulsatile gonadotropin and preovulatory gonadotropin surge, have been identified in the mammals. Pulsatile gonadotropin secretion is modulated by the pulsatile pattern of GnRH release and complex ovarian steroid feedback actions. The neural mechansim that regulates the pulsatile release of GnRH in the hypothalamus is called "GnRH pulse generator". Ovarian steroids, estradiol and progesterone, appear to exert thier feedback effects both directly on the pituitary to modulate gonadotropin release and on a hypothalamic site to modulate GnRH release; estradiol primarily affects the amplitude while progesterone decreases the frequency of the pulsatile GnRH. Steroid hormones are known to affect catecholamine transmission in brain. MBH-POA is richly innervated by NE systems and close apposition of NE terminals and GnRH cell bodies occurs in the MBH as well as in the POA. NE normally facilitates pulsatile LH release by acting through ${\alpha}-receptor$ mechanism. However, precise nature of facilitative role of NE transmission in maintaining pulsatile LH has not been clearly understood. Close apposition of DA and GnRH terminals in ME might permit DA to influence GnRH release. Action of DA transmission probably is mediated by axo-axonic contacts between GnRH and DA fibers in the ME. Dopamine transmission does not normally regulate pulsatile LH release, but under certain conditions, increased DA transmission inhibit LH pulse. Endogenous opioid acts to suppress the secretion of GnRH into hypophysial portal circulation, thereby inhibiting gonadotropin secretion. However, an interaction between endogenenous opioid peptides and gonadotropin release is a complex one which involves ovarian hormones as well. LH secretion appears to be most suppressed by endogenenous opioids during the luteal phase, at a time of elevated progesterone secretion. The arcuate nucleus contains not only cell bodies for GnRH and ${\beta}-endorphin$ but also a dense aborization of fibers suggesting that GnRH release is changed by the interactions between GnRH and ${\beta}-endorphin$ cell bodies within the arcuate nucleus. The frequency and amplitude of pulsatile LH release seem to be increased during the preovulatory gonadotropin surge. Estradiol exerts positive feedback action on the hypothalamo-pituitary axis to trigger preovulatory LH surge. GnRH is also crucial hormonal stimulus for preovulatory LH surge. It is unlikely, however, that increased secretion of GnRH during the preovulatory gonadotropin surge represents an obligatory neural signal for generation of the LH discharge in primates including human. Modulation of preovulatory LH surge by catecholamines has been studied almost exclusively in rats. NE and E may be involved in distinct way to accumulate GnRH in the MBH and its release into the hypophysial portal system during the critical period for LH surge on proestrus in rats. However, the mechanisms whereby augmented adrenergic transmission may facilitate the formation and accumulation of GnRH in the ME-ARC nerve terminals before the LH surge have not been clearly understood.

• Proceedings of the KSME Conference
• /
• 2001.06e
• /
• pp.748-753
• /
• 2001

The objective of the present study is to visualize the pulsatile flow fields by using three-dimensional computer simulation and the PIV system. A closed flow loop system was built for the steady and unsteady experiments. The Harvard pulsatile pump was used to generate the pulsatile pressure and velocity waveforms. Conifer powder as the tracing particles was added to water to visualize the flow field. Two consecutive particle images were captured by a CCO camera for the image processing at several cross section. The range validation and the area interpolation methods were used to obtain the final velocity vectors with high accuracy. The finite volume predictions were used to analyze three-dimensional flow patterns in the bifurcation model. The results of the PIV experiment and the computer simulation are in good agreement and the results show the recirculation zones and formation of the paired secondary flow distal to the apex of the bifurcated model. The results also show that the branch flow is pushed strongly to the inner wall due to the inertial force effect and helical motions are generated as the flow proceeds toward the outer wall.

• Proceedings of the KSME Conference
• /
• 2001.11b
• /
• pp.318-323
• /
• 2001

Characteristics of the pulsatile flow in a 3-dimensional elastic blood vessel are investigated to understand the blood flow phenomena in the human body arteries. In this study, a model for the elastic blood vessel is proposed. The finite volume prediction is used to analyse the pulsatile flow in the elastic blood vessel. Variations of the pressure, velocity and wall shear stress of the pulsatile flow in the elastic blood vessel are obtained. The magnitudes of the velocity waveforms in the elastic blood vessel model are larger than those in the rigid blood vessel model. The wall shear stresses on the elastic vessel vary with the blood vessel motions. Amplitude indices of the wall shear stress for blood in the elastic blood vessel are $4\sim5$ times larger than those of the Newtonian fluid. As the phase angle increased, point of the phase angle is are moved forward and the wall shear stresses are increased for blood and the Newtonian fluid.

• Asian-Australasian Journal of Animal Sciences
• /
• v.14 no.9
• /
• pp.1209-1215
• /
• 2001

We conducted this study to investigate 24 h leptin profiles and to ascertain whether leptin secretion occurs in a pulsatile manner in cattle. Plasma leptin concentrations were measured every 10 min for 24 h in five Holstein steers aged 10 months. Simultaneously, feeding behavior was recorded every 5 min during this experiment. In two of the five cattle, leptin showed diurnal rhythmicity, which could be described by a cosine, with peaks between 15:00 and 16:00 and nadirs at around midnight. Pulsatile leptin release was quantified by model-free Cluster analysis. Plasma leptin showed a pulsatile pattern in all cattle, with an average number of pulses at 15 peaks/24 h. The daily number of pulses was not related to total time spent eating, ruminating or chewing. However, when divided into six 4 h time intervals, time spent ruminating was positively related with pulse number (p=0.05) in cattle showing no diurnal plasma leptin variation. These results suggest that cattle may have unique diurnal variation and pulsatile patterns of plasma leptin, differing from those of monogastric animals.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.26 no.1
• /
• pp.39-44
• /
• 2002

The arterial stenosis related to the intimal thickening of the arterial wall is the main cause of many diseases in human cardiovascular system. Hemodynamic behavior of the blood flow is influenced by the presence of the arterial stenosis. In this study, effects of the pulsatile flow, caused by the periodic motion of the heart, on the blood flow and its interaction with the arterial stenosis are analyzed by the FEM-based computational fluid dynamics. As a result, it was found that the characteristics of the pulsatile flow in the artery with stenosis are quite different from those of the steady flow. And, the pulsatile flow condition affects the wall shear stress, which is one of the most important physiological parameters in the hemodynamics.

• Journal of the Korean Society of Visualization
• /
• v.17 no.2
• /
• pp.10-16
• /
• 2019

Considering the role of viscosity in the hemorheology, the characteristics of non-Newtonian fluid are important in the pulsatile blood flows. Stenosis, with an abnormal narrowing of the vessel, contributes to block blood flows to downstream tissue and lead to plaque rupture. Therefore, systematic analysis of blood flow around stenosed vessels is crucial. In this study, non-Newtonian behaviors of blood analog fluids around the micro-stenosis with 60 % severity in diameter of $500{\mu}m$ was examined by using CFX under the pulsatile flow conditions with the period of 10 s. Viscosity information of two non-Newtonian fluids were obtained by fitting the value of normal blood and highly viscous blood. As the Newtonian fluid, the water at room temperature was used. During the pulsatile phase, wall shear stress (WSS) is highly oscillated. In addition, high viscous solution gives rise to increases the variation in the WSS around the micro-stenosis. Highly oscillating WSS enhance increasing tendency of plaque instability or rupture and damage of the tissue layer. These results, related to the influence on the damage to the endothelium or stenotic lesion, may help clinicians understand relevant mechanisms.

• Journal of Chest Surgery
• /
• v.37 no.1
• /
• pp.1-10
• /
• 2004

Background: Currently, the cardiopulmonary machine with non-pulsatile pumps, which are low in internal circuit pressure and cause little damage to blood cells, is widely used. However, a great number of experimental studies shows that pulsatile perfusions are more useful than non-pulsatile counterparts in many areas, such as homodynamic, metabolism, organ functions, and micro-circulation. Yet, many concerns relating to pulsatile cardiopulmonary machines, such as high internal circuit pressure and blood cell damage, have long hindered the development of pulsatile cardiopulmonary machines. Against this backdrop, this study focuses on the safety and effectiveness of the pulsatile cardiopulmonary machines developed by a domestic research lab. Material and Method: The dual-pulsatile cardiopulmonary bypass experiment with total extracorporeal circulation was conducted on six calves, Extracorporeal circulation was provided between superior/inferior vena cava and aorta. The membrane oxygenator, which was placed between the left and right pumps, was used for blood oxygenation. Circulation took four hours. Arterial blood gas analysis and blood tests were also conducted. Plasma hemoglobin levels were calculated, while pulse pressure and internal circuit pressure were carefully observed. Measurement was taken five times; once before the operation of the cardiopulmonary bypass, and after its operation it was taken every hour for four hours. Result: Through the arterial blood gas analysis, PCO2 and pH remained within normal levels. PO2 in arterial blood showed enough oxygenation of over 100 mmHg. The level of plasma hemoglobin, which had total cardiopulmonary circulation, steadily increased to 15.87 $\pm$ 5.63 after four hours passed, but remained below 20 mg/㎗. There was no obvious abnormal findings in blood test. Systolic blood pressure which was at 97.5$\pm$5.7 mmHg during the pre-circulation contraction period, was maintained over 100 mmHg as time passed. Moreover, diastolic blood pressure was 72.2 $\pm$ 7.7 mmHg during the expansion period and well kept at the appropriate level with time passing by. Average blood pressure which was 83$\pm$9.2 mmHg before circulation, increased as time passed, while pump flow was maintained over 3.3 L/min. Blood pressure fluctuation during total extracorporeal circulation showed a similar level of arterial blood pressure of pre-circulation heart. Conclusion: In the experiment mentioned above, pulsatile cardiopulmonary machines using the doual-pulsatile structure provided effective pulsatile blood flow with little damage in blood cells, showing excellence in the aspects of hematology and hemodynamic. Therefore, it is expected that the pulsatile cardiopulmonary machine, if it becomes a standard cardiopulmonary machine in all heart operations, will provide stable blood flow to end-organs.

• Proceedings of the KSME Conference
• /
• 2008.11a
• /
• pp.1484-1486
• /
• 2008

Characteristics of pulsatile flow in 3-dimensional elastic vessel wall should be investigated in order to understand the physiological blood flow in human body. In this study, the modelling of the physiological blood flow in the elastic blood vessel is proposed. Variation of the pressure and the velocity wavefroms are obtained using the FSI method