• Title/Summary/Keyword: Pull-in

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Human Intersectin 2 (ITSN2) binds to Eps8 protein and enhances its degradation

  • Ding, Xiaofeng;Yang, Zijian;Zhou, Fangliang;Hu, Xiang;Zhou, Chang;Luo, Chang;He, Zhicheng;Liu, Qian;Li, Hong;Yan, Feng;Wang, Fangmei;Xiang, Shuanglin;Zhang, Jian
    • BMB Reports
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    • v.45 no.3
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    • pp.183-188
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    • 2012
  • Participates in actin remodeling through Rac and receptor endocytosis via Rab5. Here, we used yeast two-hybrid system with Eps8 as bait to screen a human brain cDNA library. ITSN2 was identified as the novel binding factor of Eps8. The interaction between ITSN2 and Eps8 was demonstrated by the in vivo co-immunoprecipitation and colocalization assays and the in vitro GST pull-down assays. Furthermore, we mapped the interaction domains to the region between amino acids 260-306 of Eps8 and the coiled-coil domain of ITSN2. In addition, protein stability assays and immunofluorescence analysis showed ITSN2 overexpression induced the degradation of Eps8 proteins, which was markedly alleviated with the lysosome inhibitor NH4Cl treatment. Taken together, our results suggested ITSN2 interacts with Eps8 and stimulates the degradation of Eps8 proteins.

Bond Properties of GFRP Rebar with Cover Thickness and Volume Fraction of Steel Fiber (강섬유 혼입률과 피복두께에 따른 GFRP 보강근의 부착특성)

  • Choi, Yun-Chul
    • Journal of the Korea Concrete Institute
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    • v.24 no.6
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    • pp.761-768
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    • 2012
  • The purpose of this study is to investigate bond properties of GFRP used in SFRC (Steel fiber reinforced concrete) and normal concrete. The experimental variables were rebar diameter (D13, D16), steel fiber volume fraction (0~2%) and cover thickness ($1.5d_b$, $5.4d_b$). The experimental results showed a different failure mode depending on the cover thickness. Through the tested specimens, splitting failure occurred for the specimens with small cover thickness and pull out failure occurred in the specimens with large cover thickness. Introduction of steel fiber caused the specimens to have more ductile behavior of bond stresss-lip after peak stress, but they did not increase the bond strength significantly. These failure modes were shown in both steel reinforcement and GFRP. However, from the difference of micro structure of bond failure mechanism between steel rebar and GFRP rebar, more ductile behavior was observed in GFRP-specimens after maximum bond strength was reached.

Failure Characteristics of Carbon/BMI Sandwich Composite Joint under Pull-out Loading (풀아웃 하중을 받는 카본/BMI 샌드위치 복합재 체결부 파손특성 연구)

  • Lee, Gyeong-Chan;Choi, Young-Ho;Lee, Kowan-Woo;Sim, Jae-Hoon;Jung, Young-In
    • Composites Research
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    • v.30 no.2
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    • pp.132-137
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    • 2017
  • The purpose of this paper is to investigate failure characteristics of Carbon/BMI-Nomex honeycomb sandwich on design parameters. A total of 6 types sandwich specimens were manufactured according to core height, face thickness and density, and environmental condition were applied to evaluate temperature and humidity effects of one of these specimens. The test results show that the core shear buckling loads was commonly observed in all specimens except for the joint with density of $64kg/m^3$. After core shear buckling, however, the joint carried additional loads over the buckling loads and then finally failed in the upper face and lower face at the same time. In the case of specimen having high stiffness, the maximum failure load was low due to interfacial failure of the upper face and core without initial core shear buckling. The ETW1 and ETW2 conditions, which were carried out to evaluate the environmental condition of the sandwich specimen, show an initial failure mode which was significantly different from RTD condition. Also, the ETW2 condition with increased temperature under the same humidity shows that the core shear buckling load was 18% less than ETW1 condition.

Properties of Lead-free Solder Joints on Flexible Substrate for Automotive Electronics (자동차 전장을 위한 플렉시블 기판 무연 솔더 접합부 특성)

  • Ahn, Sungdo;Choi, Kyeonggon;Park, Dae Young;Jeong, Gyu-Won;Baek, Seungju;Ko, Yong-Ho
    • Journal of the Microelectronics and Packaging Society
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    • v.25 no.2
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    • pp.25-30
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    • 2018
  • Sn-Pb solder has been used in automotive electronics for decades. However, recently, due to the environmental and health concerns, some international environmental organizations such as the end-of-life vehicle (ELV) enacted legislation banning of the Pb usage in automotive electronics. For this reason, many studies to develop and promote Pb-free soldering have been significantly reported. Meanwhile, because of flexibility and lightweight, flexible printed circuit boards (FPCBs) have been increasingly used in automotive electronics for lightweight to improve fuel efficiency and space utilization. Although the properties of lead-free solders for automotive electronics have been widely studied, there is a lack of research on the reliability performance of the lead-free solder joint on FPCB under user conditions. This study reported the properties of solder joints between Pb-free solders such as Sn3.0Ag0.5Cu, Sn0.7Cu and Sn0.5Cu0.01Al (Si), and various FPCBs finished with organic solderability preservative (OSP) and electroless nickel immersion gold (ENIG). To evaluate on joint properties and reliabilities with different solder compositions and surface-finishing materials, pull strength test, thermal shock test, and bending cycle test were performed and analyzed. After the bending cycle test of solder joint on OSP-finishing, the fractures were occurred in solder and the lifetime of Sn3.0Ag0.5Cu solder joint was the longest.

Complications of Femoral Lengthening Using Monolateral External Fixator in Rabbits (토끼에서 단측성 외고정기구를 이용한 대퇴골 신연술의 합병증)

  • Song, Hae-Ryong;Hwang, Jae-Min;Koo, Ja-Min;Kim, Hyeon-Hui;Lee, Won-Ik;Kim, Gon-Sup;Lee, Hyo-Jong;Chang, Ki-Churl;Yeon, Seong-Chan
    • Korean Journal of Veterinary Research
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    • v.42 no.3
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    • pp.411-418
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    • 2002
  • Thirty rabbits underwent femoral lengthening using monolateral external fixator to evaluate results and complications of limb lengthening. Twenty rabbits survived until consolidation of callus formed at the lengthening site after finishing lengthening. Ten rabbits were sacrificed during femoral lengthening because of complications. Survived 20 rabbits were classified to two groups according the amount of lengthening: group I (10% lengthening of the femoral length); group II (20% lengthening). There was no significant difference of consolidation time between two groups (p=0.25). Varus angulation at the lengthening site occurred in 60% of two groups and the amount of varus angulation in group II was larger than that of group I. Degenerative change of articular cartilage at the medial condyle of the distal femur was found in 30% of group II. Of sacrificed ten rabbits, 5 had pin loosenings with pull an of pins from the femur, 2 had fermoral fractures around the pin-tract site, and 3 had severe osteomyelitis of the femur around the pin-tract site.

Comparison of Heat Insulation Characteristics of Multi-layer Thermal Screen and Development of Curtain System (다겹보온자재의 보온성 비교 및 커튼개폐장치 개발)

  • Lee, Si-Young;Kim, Hark-Joo;Chun, Hee;Yum, Sung-Hyun;Lee, Hyun-Joo
    • Journal of Bio-Environment Control
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    • v.16 no.2
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    • pp.89-95
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    • 2007
  • This study was accomplished to compare energy saving effects of several heat insulation materials in greenhouse and to develop new automatic opening and closing equipment which is suitable to the most effective heat insulation material. To find out more effective heat insulation material, the magnitude of heat transfer occurred through aluminum screen (ALS), non-woven fabric (NWF), double-layer aluminum screen with chemical cotton sheet (DAL), and multi-layer fabric screen material quilted with non-woven fabric, chemical cotton, poly foam, and polypropylene (MLF) were compared relatively. The results showed that the relative magnitude of heat transfer occurred through MLF was lower than DAL and ALS by 23.3% and 43.0% respectively. MLF screen material was the most effective compared with other heat insulation materials. But because of thickness, there was a need of new mechanism for automatic operation in greenhouse. Accordingly, new screen system using MLF-thick but profitable for keeping warm in greenhouse-was developed. Opening & closing equipment was designed to roll MLF with pipe axis during opening process and pull MLF with string during closing process with electric motors, clutches, drums, and so on. In hot pepper cultivation and energy saving test during winter time, the early stage yield of pepper under MLF screen system was higher than NWF by 27%, and gasoline consumption of MLF screen system was lower than NWF by 46%.

The Design of the Class E Swiching Frequency Multiplier (스위칭 모드 E급 주파수 체배기 설계)

  • Roh, Hee-Jung;Seo, Choon-Weon
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.23 no.10
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    • pp.90-99
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    • 2009
  • In this paper, we proposed the new class-E frequency multiplier design that include the highest efficient characteristics. The proposed frequency multiplier is designed for 5.8[GHz] output using the frequency multiplier about 2.9[GHz] input signal. And studying in this paper is for the design and the implementation of the class E frequency multiplier. For the result, the maximum highest efficient characteristics 32[%] which is with output power 24.5[dBm] and 8.5[dB], is shown with frequency multiplier for the 2.9/5.8[GHz] class E. And we applied the linear method to the implemented class E frequency multiplier. As a result, the output spectrum for the linear is upgrade to 12[dB], 12[dB], 13[dB] of the ACPR characteristics on the +11[MHz], +20[MHz], +30[MHz] offset frequency in the center frequency. The result is satisfied with the 3.83[%] of the lineared EVM for the 64-QAM modulated method with the 54[Mbps] transmission velocity. In this paper, we show that the good compensation result of the linearity and the efficiency through the digital pre-linear method of the distortion with the frequency multiplier. Therefore, we suggested the frequency multiplier method are applying to WLAN, cellular, PCS, WCDMA, and etc.

Factors Associated with Radiologic Tests in Patients with Radial Head Subluxation (요골두아탈구 환자에서 방사선 검사 시행과 연관된 인자 분석)

  • Chang, Ikwan;Kim, Do Kyun;Park, So Young;Suh, Dongbum;Jung, Jae Yun;Kwak, Young Ho
    • Journal of Trauma and Injury
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    • v.27 no.2
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    • pp.13-19
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    • 2014
  • Purpose: In general, X-ray examinations are not recommended for radial head subluxation (pulled elbow) patients. The purpose of this study was to determine the frequency of X-ray examinations and to investigate the factors associated with the decision to perform an X-ray examination on a patient with a pulled elbow. Methods: Patients who visited the pediatric emergency department (ED) of one tertiary hospital from January 1, 2011, to December 31, 2012, with a diagnosis of radial head subluxation at discharge were enrolled in this study. Through retrospective chart reviews, factors that could have influenced the decision to perform an X-ray examination and their statistical relevance were analyzed. Results: A total 308 patients were enrolled, and 101 patients (32.8%) underwent X-ray examinations. Among the 252 patients with a typical pulled elbow, 65 underwent X-ray examination. This result showed statistical significance compared to atypical pull-elbow group (25.8% vs. 64.3%, p<0.001). Factors associated with the decision to perform an X-ray examination were analyzed using the Chi-square test and the Fisher's exact test. The mechanism of injury and consultation with an orthopedic surgeon (p=0.001) showed statistical significance. In the multivariable logistic regression, the odds ratio (OR) for the injury mechanism was 4.7 (95% CI: 1.8~8.8, p<0.001) and that for consultation with an orthopedic surgeon was 8.0 (95% CI: 1.6~40.7, p=0.004). Conclusion: One third of patients with a pulled elbow underwent X-ray examination, and patients with an atypical mechanism of injury underwent more frequent X-ray examinations than did patients with a typical mechanism of injury. The factors that could have influenced the decision to perform an X-ray examination were mechanism of injury and consultation with an orthopedic surgeon.

Magnetic resonance imaging Usefulness after Medial Meniscus Posterior Root Tear Repair (내측 반월상 연골판 후각 기시부 파열 봉합 후 추시 자기공명영상 검사의 유용성)

  • Chon, Jegyun;Kim, Jun-Beom;Lee, Bong-Ju
    • Journal of the Korean Arthroscopy Society
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    • v.17 no.1
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    • pp.6-10
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    • 2013
  • Purpose: This study intends to verify the usefulness of magnetic resonance imaging (MRI) for estimate recovery after arthroscopic pull-out repair at root tears of medial meniscus. Materials and Methods: We performed 17 patients who examined MRI and arthroscopy among patients who had received repair of medial meniscus from November, 2007 to June, 2011. To determine restoration meniscus, we performed arthroscopy and MRI. Results: Lysholm knee scores before and after operation were average 56.4 and 79.0 and visual analogue scale (VAS) score was improved from 8 points to 3 points. From secondary look arthroscopy performed after operation, 17 cases showed stabilization after regeneration. However, In MRI, cleft sign implying root tears of medial meniscus was observed in all cases before and after operation, ghost sign was observed in 10 cases and 9 cases respectively, radial linear defect was showed 17 cases and 15 cases respectively. Conclusion: It was not useful that MRI after medial meniscus repair in non-anatomical site, to consider restoration of medial meniscus. To evaluate for recovery medial meniscus after repair more exactly, secondary arthroscopy would be required.

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THE ERUPTION GUIDANCE OF IMPACTED MAXILLARY ANTERIOR TEETH (맹출장애를 보이는 상악 전치의 맹출유도)

  • Sim, Jeung-Ho;Eum, Jong-Hyeok;Kim, Shin;Jeong, Tae-Sung
    • Journal of the korean academy of Pediatric Dentistry
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    • v.31 no.1
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    • pp.34-40
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    • 2004
  • Unerupted maxillary anterior teeth is not a common case, However it may present practitioners with management problem. The cause of impaction is considered to be multifactorial, and local cause is the most common. These impacted teeth require surgical intervention, removal, transplantation, or surgical exposure, with or without orthodontic traction to align the malpositioned tooth. The preferred option is surgical exposure and orthodontic correction. Surgical intervention and orthodontic correction should not be delayed to avoid unnecessary difficulties in aligning the tooth in the arch. Surgical exposure should be performed with the intent of providing sufficient attached gingiva rather than simply uncovering the crown, which results in only alveolar mucosal attachment. Attached gingiva is essential to secure the gingival tissues to the adjacent teeth at the dentogingival junction. Thus preventing loss of periodontal tissues as a result of the pull of the surrounding soft tissues and facial muscles. Labially impacted maxillary anterior teeth uncovered with an apically positioned flap technique have more un- esthetic sequelae than those uncovered with a closed-eruption technique. In the case of severly displaced impacted teeth, autotransplantation ensures preservation of the alveolar bone and will facilitate future placement of an osseointegrated implant once growth has ceased or if ankylosis/resorption of the transplant occurs.

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