• Animal cells and systems
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• v.8 no.1
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• pp.49-55
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• 2004

In vertebrates, multisubunit cofactors regulate gene expression through interacting with cell-type- and gene-specific DNA-binding proteins in a chromatin-selective manner. ADD1/SREBP1c regulates fatty acid metabolism and insulin-dependent gene expression through binding to SRE and E-box motif with dual DNA binding specificity. Although its transcriptional and post-translational regulation has been extensively studied, its regulation by interacting proteins is not well understood. To identify cellular proteins that associate with nuclear form of ADD1/SEBP1c, we employed the GST pull-down system with Hela cell nuclei extract. In this study, we demonstrated that Ku proteins interact specifically with ADD1/SREP1c protein. GST pull-down combined with peptide sequencing analysis revealed that Ku80 binds to ADD1/SREBP1c in vitro. Additionally, western blot analysis showed that Ku70, a heterodimerizing partner of Ku80, also associates with ADD1/SREBP1c. Furthermore, co-transfection of Ku70/Ku80 with ADD1/SREBP1c enhanced the transcriptional activity of ADD1/SREBP1c. Taken together, these results suggest that the Ku proteins might be involved in the lipogenic and/or adipogenic gene expression through interacting with ADD1/SREBP1c.

• Journal of the Ergonomics Society of Korea
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• v.22 no.2
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• pp.57-69
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• 2003

This study was performed to investigate the isometric endurance time as percentages of maximum voluntary contraction. Electromyogram(EMG) and Borg's CR-I0 value were measured by push-pull-up-down tasks for 10 healthy males. The normalized EMG value and the MPF(mean power frequency) were used to estimate the muscle recruitment pattern and the development of muscle fatigue. The subjects exerted and maintained 5 levels of %MVC(maximum voluntary contraction) in $90^{\circ}$ shoulder flexion/ 180oelbow extension at sitting posture. The up-task showed the lower endurance time and higher Borg's CR-I0 value than the other task types. Comparing Rohmert's curve with the endurance time of task types. Rohmert's curve overestimated the endurance time of up-task and underestimated the endurance time of push-pull-down tasks. The normalized EMG value showed that muscles recruitment patterns were different from task types. The 4 muscles(biceps brachii muscle, tricep brachii muscle. middle deltoid muscle. trapezius muscle) recruitment patterns of up-task were higher than those of other tasks. The MPF value decreased with the endurance time, and the shift of MPF at up-task was larger than that of the other task types.

• BMB Reports
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• v.38 no.4
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• pp.373-380
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• 2005

For measles viruses, fusion on the cell membrane is an important initial step in the entry into the infected cells. The recent research indicated that hemagglutinin firstly leads the conformational changes in the fusion protein then co-mediates the membrane fusion. In the work, we use the co-immunoprecipitation and pull-down techniques to identify the interactions among fusion protein, hemagglutinin and signaling lymphocyte activation molecule (SLAM), which reveal that the three proteins can form a functional complex to mediate the SLAM-dependent fusion. Moreover, under the confocal microscope, fusion protein and hemagglutinin protein can show the cocapping mediated by the SLAM. So fusion protein not only is involved in the fusion but also might directly interact with the SLAM to be a new fusion-trimer model, which might account for the infection mechanism of measles virus.

• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.3
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• pp.192-198
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• 2003

BiP, immunoglobulin binding protein, is an ER homologue of Hsp70. However, unlit other Hsp70 proteins, regulatory protein(s) for BiP has not been identified. Here, we demo strafed the presence of potential regulatory proteins for BiP using a pull -down assay. Since BiP can bind any unfolded protein, only the ATPase domain of BiP was used for the pull -down assay in order to minimize nonspecific binding. The ATPase domain was cloned to produce recombinant protein, which was then conjugated to CNBr-activated agarose. The structural conformation and ATP hydrolysis activity of the recombinant ATPase domain were similar to those of the native protein, light proteins from metabolically labeled mouse plasmacytoma cells specifically bound to the recombinant ATPase protein. The binding of these proteins was inhibited by excess amounts of free ATPase protein, and was dependent on the presence of ATP. These proteins were eluted by ADP. Of these proteins, Grp170 and BiP where identified. while the other were not identified as known ER proteins, from Western blot analyses. The presence of the ATPase-binding proteins for BiP was first demonstrated in this study, and our data suggest similar regulatory machinery for BiP may exist in the ER, as found in prokaryotes and other cellular compartments.

• Bulletin of the Korean Chemical Society
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• v.25 no.12
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• pp.1863-1868
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• 2004

Kringle 5 (K5), located outside of angiostain (K1-4) in human plasminogen, displays more potent antiangiogenic activity on endothelial cell proliferation than angiostatin itself. Using a yeast two-hybrid system in vivo, we have recently identified Rgl2 (guanine nucleotide dissociation stimulator (RalGDS)-like factor 2) as a binding protein of human K5. In order to confirm in vitro protein interaction between K5 and Rgl2, we developed bacterial recombinant expression systems for them. K5 and Rgl2 proteins were expressed in high yields and purified into pure forms with His tags and GST fusion, respectively. GST-pull down experiments clearly demonstrated that K5 interacts specifically with Rgl2 in vitro. These results indicate that Rgl2 functions as a receptor protein for K5 in vitro as well as in vivo, leading to anti-angiogenesis through regulating Ras signaling pathways.

• Design & Manufacturing
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• v.10 no.2
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• pp.29-33
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• 2016

Production machines have been more important, due to quality level of vehicle motor core is getting higher. That is why, to improve assembly fit of tooling and to be emphasized how much moving down caused of deterioration of high speed press, it is also getting more important parts as solution of problems. To analyze how much move based on condition of movement as tooling and high speed press, and to measure how much impact to embossing height caused of changing movement down. As the result of investigation, in case of material thickness 0.5mm, there is highest pull and force power when emboss height is 0.45mm. If emboss height is less than 0.45mm, pull and force power is getting lower, if emboss height is higher than 0.45mm, it is impossible to make it forming caused of changed press movement, also it has been piercing.

• Archives of design research
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• v.17 no.1
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• pp.127-134
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• 2004

The purpose of this study was to compare data input interfaces used in e-business applications on the web and find optimal input design characteristics. Basic data entry tools such as a pull down menu, list, text input box, and radio button were examined by inputting data into a simulated hotel room reservation web site. Experimental results indicated that the text input box was most efficient for experts or experienced operators when there are more than four menu-items and pull down menu was considered most satisfactory, simplest, and easier to use for novices or unexperienced users. A simple list was determined to be the best for the input of binary data considering user's satisfaction, simplicity, and flexibility but radio button was evaluated best for the ease to use. Design guide lines of this study can be applied to build a usable interactive web sites and increase economic efficiency.

• The Korean Journal of Pesticide Science
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• v.12 no.2
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• pp.162-167
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• 2008

To enhance the control efficacy against cockroaches in dwelling space, repellent ("Push") was set down on one comer and a poison bait including attractant ("Pull") was on the opposite side. And we tested the control efficacy of Push-Pull strategy by behavior-stimuli of cockroaches. In a mini-field test, German cockroach males primarily chose shelters nearest to the attractant-treated surfaces and farthest from the repellent-treated surfaces. The consumption of food or bait was also highest from food nearest to the preferred shelters by push-pull effect. We evaluated the push-pull insecticidal efficacies of five essential oils belong to Citrus plant, namely, grapefruit, lemon, lime, orange, and petitgrain. The combined push-pull treatments appeared to be faster and higher insecticidal effects than single (pull) treatment, and the repellent efficacy of Citrus oils was in the order of grapefruit > lemon > lime > orange > petitgrain. Therefore, we propose the effective control of the german cockroach using Citrus oils as repellents and push-pull method into a limited area.

• Asia pacific journal of information systems
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• v.8 no.3
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• pp.181-194
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• 1998

The inability of the management information systems (MIS) field to progress as a scientific discipline has been attributed to the lack of systematic research and a cumulative tradition, an identity crisis, and the poverty of scientism. While research on the status of MIS is very important in order to enhance the field as a scientific discipline, few have investigated this issue. Following Thomas Kuhn's idea of paradigm, this paper studies other fields to investigate when they progressed, when they did not, and why. After research paradigm was broken down into technology-push and demand-pull types, a model on the science life cycle was developed in an effort to explain the path how a science has progressed. A test of this model in the fields of physics and chemistry with an old historial background reveals that the scientific progress in the area of demand-pull is more desirable if this progress turns out to be in the right direction. An application of the model to the MIS field shows that the research paradigm in this field is mainly of technology-push. In order to shift this paradigm toward the demand-pull area, this paper suggests the research on the relationship among MIS subfields and the adoption of appropriate reference disciplines.

• Journal of Biomedical Engineering Research
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• v.9 no.2
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• pp.247-250
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• 1988

Laboratory information system (LIS) is a key tool to manage laboratory data in clinical pathology. Our department has developed an information system for routine hematology using down-sized computer system. We have used an IBM 486 compatible PC with 16MB main memory, 210 MB hard disk drive, 9 RS-232C port and 24 pin dot printer. The operating system and database management system were SCO UNIX and SCO foxbase, respectively. For program development, we used Xbase language provided by SCO foxbase. The C language was used for interface purpose. To make the system use friendly, pull-down menu was used. The system connected to our hospital information system via application program interface (API), so the information related to patient and request details is automatically transmitted to our computer. Our system interfaced with fwd complete blood count analyzers(Sysmex NE-8000 and Coulter STKS) for unidirectional data tansmission from analyzer to computer. The authors suggests that this system based on down-sized computer could provide a progressive approach to total LIS based on local area network, and the implemented system could serve as a model for other hospital's LIS for routine hematology.