• Title/Summary/Keyword: Pueraria thunbergiana

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A Study on the Estrogenicity of Korean Arrowroot (Pueraria thunbergiana) (한국산 칡의 Estrogen 활성에 관한 연구)

  • Kim, So-Jung;Park, Chul;Kim, Hae-Gyoung;Shin, Wan-Chul;Choe, Suck-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.1
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    • pp.16-21
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    • 2004
  • To assess the estrogenicities of Korean arrowroot (Pueraria thunbergiana) the contents of nine phytochemicals which are known to present were analyzed by HPLC. Also the estrogenecities of these phytochemicals were assayed using estrogen receptor dependent transcriptional expression assay. Daidzein and puerarin are major compounds in arrowroot, which were contained in the order of: root>stem>leaf>flower>seed. Among the assayed phytochemicals daidzein, genistein, biochanin A, formononetin, puerarin and genistin were highly estrogenic. The total estrogenicities in different parts of arrowroot and those by regions in Korea were also assessed. Roots had the highest estrogenic effects. The estrogenicities also were shown in stem and leaf. The differences in the estrogenicities of arrowroots by regions in Korea were shown. These results demonstrated that the estrogenic phytochemicals content was the highest in Korean arrowroot and also present in stem and leaf.

Anti-obesity Effects of Capsule-filled Cheonggukjang Added with Arrowroot (Pueraria thunbergiana) Extracts in ob/ob Mice (칡(Pueraria thunbergiana)추출물 청국장 캡슐의 ob/ob Mice에 대한 항비만 효과)

  • Kim, Kil-Soo;Hong, Joo-Heon;Kim, Dae-Ik
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.6
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    • pp.782-789
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    • 2012
  • This study was designed to evaluate the anti-metabolic syndrome effects of capsule-filled cheonggukjang (CGJ) added with arrowroot (Pueraria thunbergiana) extracts on body weight, adiposity and lipid metabolism in ob/ob mice. Experimental groups were normal control group (NC: basal diet), positive control group (PC: 2% CGJ), CGJ added with arrowroot extracts group (AR: 2% arrowroot in CGJ), and capsule-filled CGJ added with arrowroot extracts group (ARC: 2% arrowroot CGJ capsule). Each group was fed experimental diet for 10 weeks. Final body weight gain and atherogenic index were significantly lower in the ARC than NC group. Serum levels of total cholesterol, LDL-cholesterol, and triglycerides, blood glucose and atherogenic index were significantly lower in the ARC than NC group. Furthermore, fatty liver and regional lipid accumultion in ob/ob mice were inhibited in the ARC group. The hepatic activities of superoxide dismutase, catalase and glutathione S-transferase were significantly higher in the ARC than NC group. Therefore, the anti-matabolic syndrome effects of the ARC group were higher than the AR group. In conclusion, these results indicated that CGJ added with arrowroot mediates its anti-obesity effects in ob/ob mice by improving lipid metabolism and antioxidant enzyme.

Vegetation Structure and Succession of Highway Cutting-slope Area (고속도로 절토비탈면의 식생구조와 천이)

  • Song, Hokyung;Jeon, Giseong;Lee, Sanghwa;Kim, Namchoon;Park, Gwansoo;Lee, Byungjun
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.8 no.6
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    • pp.69-79
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    • 2005
  • This study was carried out to select proper species for early stage replantation in highway cut-slope area. In highway cut-slope area, sample plots of 106 were selected, and their vegetations and environmental factors were investigated. 1. We found total 172 species in the 106 cutting area of highway. The species of high frequency of highway cut-slope were found in the order of Lespedeza bicolor, Artemisia princeps var. orientalis, Festuca arundinacea, Erigeron annuus, Lespedeza cuneata, Lactuca indica var. laciniata, Eragrostis curvula, Dactylis glomerata, Oenothera lamarckiana, Wistaria floribunda, Humulus japonica, Commelina communis, Miscanthus sinensis, Pueraria thunbergiana, Pinus densiflora, etc. 2. The average vegetation coverage was over 90% in the study sites and the average coverage was 91.4% in the total cut-slope area. The species of high coverage of highway cut-slope area were found in the order of Festuca arundinacea, Eragrostis curvula, Lespedeza bicolor, Wistaria floribunda, Lespedeza cuneata, Dactylis glomerata, Artemisia princeps var. orientalis, Humulus japonica, Pueraria thunbergiana, Robinia pseudoacacia, Poa pratensis, Medicago sativa, Festuca ovina, Pinus densiflora, Parthenocissua tricuspidata, etc. 3. The total coverage in the foreign plants of Festuca arundinacea, Eragrostis curvula, Dactylis glomerata, Poa pratensis, Medicago sativa, Coreopsis drummondii and native plants of Lespedeza bicolor, Wistaria floribunda, Lespedeza cuneata, Amorpha fruticosa, Indigofera pseudotinctoria, Lespedeza cyrtobotrya were 57.52%. That is, the ecological succession of native herbs and parachute shrubs have delayed because the afforested plants occupy 57.52%. In future, the coverage of foreign herbs have to reduce, and the coverage of the native herbs and parachute shrubs must be increased. 4. The native seed of Artemisia sp., Miscanthus sinensis, Smilax china, Pueraria thunbergiana, Rubus crataegifolius, Rubus parvifolius, Pinus densiflora, Rhus chinensis, Albizzia julibrissin, Rhododendron mucronulatum, Clematis apiifolia, Zanthoxylum schinifolium, Prunus sargentii could be added in the seedling of the temperate south zone highway with the used seeds. The native seed of Artemisia sp., Miscanthus sinensis, Rubus crataegifolius, Rhododendron mucronulatum, Weigela subsessilis, Stephanandra incisa, Rhus chinensis, Pinus densiflora, Salix koreensis, Cocculus trilobus, Populus alba, Spiraea prunifolia for. simpliciflora, Clematis apiifolia, Lindera obtusiloba, Quercus serrata, etc., could be added in the seedling of the temperate middle zone highway with the used seeds. 5. We have some recommendation. The native plants have to growth in the highway cut-slope area instead of foreign plants to have good environmental ecology. The role of the foreign plants should be the plant for the initial several years in the highway cut-slope area. And, the native plants should growth in the next season. 6. We should protect shrubs and trees in the highway slope area because shrubs and trees can be more helpful in stabilizing of the slope area than herbs.

Effect of Tectorigenin Obtained from Pueraria thunbergiana Flowers on Phase I and -II Enzyme Activities in the Streptozotocin-induced Diabetic Rat

  • Choi, Jong-Won;Shin, Myung-Hee;Park, Kun-Young;Lee, Kyung-Tae;Jung, Hyun-Ju;Park, Hee-Juhn
    • Natural Product Sciences
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    • v.9 no.4
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    • pp.235-240
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    • 2003
  • Tectorigenin has an apoptosis-inducing ability and immunosuppressive activity. We investigated the effect of tectorigenin on Phase I and II enzyme activities to elucidate the pharmacological action of the immunosuppressive tectorigenin in the diabetic rat. This compound was obtained from the hydrolysis of tetoridin isolated from the flower of Pueraria thumbergiana (Leguminosae). This crude drug (Puerariae Flos) has been used as a therapeutic for diabetes mellitus in traditional Korean medicine. Tecotrigenin inhibited the formation of malondialdehyde (MDA) and hydroxy radicals in serum and liver but promoted superoxide dismutase (SOD) activity. Low MDA contents and low xanthine oxidase and aldehyde oxidase activities were observed in the tectorigenin-treated rats, suggesting that such Phase I enzyme activities are the major source of lipid peroxidation. However, tectorigenin increased Phase II enzyme activities such as SOD, glutathione peroxidase and catalase, suggesting the activation of free radical-scavenging enzymes. The activities of tectorigenin were comparable to those of glibenclamide, which was employed as a positive control. These results suggest that tectorigenin may share some biological properties with glibenclamide in insulin-dependent-diabetes mellitus (IDDM).

Pharmacological Studies on Butanol Fraction of Puerariae Radix (갈근 Butanol Fraction의 약리 작용에 관한 연구)

  • 허인회;이상준
    • YAKHAK HOEJI
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    • v.27 no.4
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    • pp.263-270
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    • 1983
  • Effective BuOH fraction was extracted from Puerariae radix (Pueraria thunbergiana), and several pharmacological activities were examined. From several pharmacological examinations, it was found that BuOH fraction has an antipyretic activity against typhoid vaccine pyrexia in rabbit, a papaverine-like antispasmodic activity on isolated ileum and jejunum of rat, an analgesic effects on mice treated by 0.7% HAc, and an anti-inflammatory activity of carrageenin edema in rat. It was found that BuOH fraction has detoxication effects on acute and subacute toxic rat induced by alcohol in consequence of biochemical tests of serum. Therefore it was considered that BuOH fraction has preventive and therapeutic effects in alcohol intoxication.

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Effect of the root extract of Pueraria thunbergiana Bentham on high fat/high sucrose diet and single low-dose streptozotocin-induced diabetic mice (갈근이 고지방·고탄수화물식이와 저용량 streptozotocin-유도 당뇨병 마우스에 미치는 효능 연구)

  • Oh, Tae Woo;Park, Yong-Ki
    • The Korea Journal of Herbology
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    • v.29 no.5
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    • pp.75-81
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    • 2014
  • Objectives : This study was performed to investigate the effect of root extract of Pueraria thunbergiana Bentham (Puerariae Radix, PR) in diabetic mice as similar as emaciation-thirst disease in Oriental medicine. Methods : C57BL/6 mice were fed high fat (HF) and high sucrose (HS) for 8 weeks, and then administrated with 90 mg/kg body weight (bw) of streptozotocin (STZ) for induction of diabetes which is similar to the middle emaciation stage. After 5 days, blood glucose levels were measured, and selected the mice with ranges above $250mg/d{\ell}$. PR water extract was administrated orally once a day for 4 weeks with high fat and high sucrose. The levels of glucose, insulin, total cholesterol, triglyceride, ${\gamma}glutamyl$ transpeptidase (${\gamma}GTP$), glutamic oxaloacetic transaminase (GOT) and glutamate pyruvate transaminase (GPT) were analysed in the serum. Also, observed their histological changes by hematoxylin and eosin (H&E) of different organs, lung, heart, pancreas, stomach, liver, and kidney. Results : PR extract significantly decreased the levels of serum glucose and insulin in diabetic mice. PR extract significantly increased the levels of triglyceride, total cholesterol, GOT and GPT in diabetic mice. In H&E stain, PR extract inhibited the histopathological changes of lung (as a channel of the upper emaciation stage in the channel-tropism theory), pancreas (as a channel of the middle emaciation stage) and kidney (as a channel of the lower emaciation stage) in diabetic damage. Conclusions : PR extract has an anti-diabetic effect in HF/HS and low-dose STZ-induced diabetic mice. This result suggests that PR follows the channel-tropism theory in the emaciation-thirst disease through the protection of lung, pancreas and kidney.

Antioxidant and Antiproliferative Activities of Methanolic Extracts from Thirty Korean Medicinal Plants

  • Choi, Young-Min;Gu, Ja-Bi;Kim, Myung-Hee;Lee, Jun-Soo
    • Food Science and Biotechnology
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    • v.17 no.6
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    • pp.1235-1239
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    • 2008
  • To study the health promoting effects of medicinal plants, 30 medicinal plants commonly available in Korea have been evaluated for their antioxidant compounds and antioxidant and antiproliferative activities. Total polyphenolics and flavonoids in the methanolic extracts were measured by spectrophotometric methods and 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activities and chelating effects have been determined for antioxidant activities. Moreover, the effects of medicinal plants on cell proliferation of intestinal (Caco-2) and pituitary (GH3) tumor cells were investigated using thiazolyl blue terazolium bromide (MIT) assay. The methanolic extracts of Pueraria thunbergiana and Artemisiae asiatria contained the highest total polyphenolic and flavonoid contents, respectively. P. thunbergiana exhibited the highest antioxidant activities. A. asiatria showed the strongest antiproliferative activity against Caco-2 and Ponciruc trifoliata Rafin and Lophathrum gracile Bronghiart exhibited the highest activities against GH3. Although there was positive correlation between ABTS radical scavenging activity and polyphenolic contents ($R^2=8189$), no relationship was found between antiproliferative and antioxidant activities.

Effects of Pueraria thunbergiana Bentham Water Extracts on Hepatic Alcohol Metabolic Enzyme System In Rats (칡 열수추출물이 흰쥐의 알콜올 대사효소계에 미치는 영향)

  • 김명주;이정수;하오명;장주연;조수열
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.1
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    • pp.92-97
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    • 2002
  • The effects of Pueraria flos (PF) and Pueraria radix (PR) water extract on the hepatic alcohol metabolic enzyme activities were examined in rats that were orally administered ethanol (25% v/v, 5g/kg body weight/day) for 5 weeks. The PF and PR water extract were supplemented in a diet, based on 1.2 g or 2.4 g of raw PF or PR/kg body weight/body. Alcohol administration without the PF or PR supplementation significantly decreased net weight gain, feed intake and feed efficiency ratio. However. both dose of the PF of PR supplementation resulted in significant enhancement of growth and suppression of increased relative weight of liver, brain and heart by alcohol administration. Activities of hepatic alcohol dehydrogenase and microsomal ethanol oxidizing system were higher in the alcohol treated group than in the normal group, while aldehyde dehydrogenase activity was significantly lowered in the alcohol treated group. The hepatic metabolic enzyme activities altered by alcohol administration were normalized by both doses of PF or PR supplement. Hepatic monoamine oxidase activity and hydrogen peroxide, which were significantly higher in the alcohol treated group than in the normal group, were also decreased by the supplementation with either PF or PR. These results indicate that low-or high-supplementation of either water extract PF or PR may alleviate ethanol-induced hepatotoxicity by altering alcohol metabolic enzyme activities.

Preparation of Pueraria thunbergiana Benth Roots Film (칡 뿌리를 이용한 필름의 제조)

  • Jeong, Hyeon-Yeop;Son, Tae-Won;Lee, Geon-Min;Choe, Won-Mi;Lee, Yeon-Jin
    • Proceedings of the Korean Society of Dyers and Finishers Conference
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    • 2008.04a
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    • pp.147-149
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    • 2008
  • 현재 칡은 잎, 줄기, 뿌리를 이용해 많은 연구가 이루어지고 있다. 칡의 줄기를 이용해 삼태기, 광주리, 바구니 등을 제작하고, 꽃으로는 갈화라하여 식욕부진, 구토, 장출혈, 술 중독 등에 처방하고 있다. 그 중 뿌리는 고열, 두통, 고혈압, 주독, 설사 등에 효과가 있어 뿌리를 달여 칡차로서 많이 음용되고 있다. 하지만 뿌리의 즙을 짜고 남은 칡뿌리 섬유의 잔사는 식물성 섬유소로서 현재 대부분 폐기되거나 일부 양축 농가에서 사료의 증량제로 활용되고 있는 실정이다. 매년 1,000톤이상의 칡뿌리 생산량 중 잔사가 600톤 이상 된다. 이 잔사를 이용해 펄프를 만들고 필름을 만들어 나아가 섬유를 만듬으로써 친환경적인 섬유로써의 가능성을 살펴보고자 한다.

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Effect of Herbal Composition on Alcohol Degradation and Anti-inflammatory Activity in Mice (생약 조성물의 알코올 분해 및 항염증 효과)

  • Kang, Tae-Bong;Yoon, Taek-Joon
    • The Korean Journal of Food And Nutrition
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    • v.24 no.4
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    • pp.489-495
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    • 2011
  • These studies were conducted to investigate the effects an herbal extract composition(MHE) containing $Hovenia$ $dulcis$ T., $Artemisa$ $capillaris$ T., $Pueraria$ $thunbergiana$ B., $Polygonatum$ $falcatum$ A., $Agastache$ $rugosa$ O., $Silybum$ $marianum$ L. and $Glycyrrhiza$ $uralensis$ F. in alcohol administered mice. Prophylactic administration of different doses of MHE(20~100 mg/kg) had beneficial actions toward alcohol degradation in acute alcohol treated mice. In addition, intraperitoneal administration of the MHE showed anti-inflammatory effects in inhibition tests of vascular permeability produced by acetic acid. MHE also reduced the concentrations of nitric oxide(NO) and tumor necrosis factor(TNF)-${\alpha}$ in macrophages that were activated by LPS. These results demonstrate that MHE possesses the potential to stimulate alcohol degradation and inhibit inflammatory effects in mice.