• Bulletin of the Korean Chemical Society
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• 제29권9호
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• pp.1755-1760
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• 2008

Energy minimization and conformational studies of molecular ions generated by ESI (electrospray ionization) tandem mass spectrometry (MS/MS) can be used for the discrimination of stereoisomeric permethylated and sodium cationized trisaccharides. Sets of fucose-containing trisaccharides having different internal and terminal linkages have been synthesized to analyze the reducing terminal linkage positions using BT and IT fusion approaches. A detailed investigation has been undertaken on the conformational behaviors of four trisaccharide fragments from human milk and blood group determinants of Type 1 and Type 2, namely Fuc($\alpha$1- 3)Gal($\beta$1-3)GalNAc and Fuc($\alpha$1-3)Gal($\beta$1-X)GlcNAc where X = 3, 4 and 6 using molecular modeling methods. Three dimensional rigid and adiabatic phi-psi-energy maps (Surfer program) describing the energy as a function of rotation around corresponding glycosidic linkages were calculated by SYBYL molecular modeling and MM4 force field programs conjunction with cleavage energies of ESI MS/MS for the side group orientations. This approach predicted conformational behaviors exhibited by isomer saccharides for future applications on biologically active glycoconjugates and to exploit a faster method of synthesizing a series of structural isomeric oligosaccharides.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1998년도 제13차 학술회의논문집
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• pp.285-288
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• 1998

A least-squares identification method is studied that estimates a finite number of coefficients in the series expansion of a transfer function, where the expansion is in terms of recently introduced generalized basis functions, We will expand and generalize the orthogonal functions as basis functions for dynamical system representations. To this end, use is made of balanced realizations as inner transfer functions. The orthogonal functions can be considered as generalizations of, for example, the pulse functions, Laguerre functions, and Kautz functions, and give rise to an alternative series expansion of rational transfer functions. We show that the Laplace transform of the expansion for some sets$\Psi_{\kappa}(Z)$ is equivalent to a series expansion . Techniques based on this result are presented for obtaining the coefficients $c_{n}$ as those of a series. One of their important properties is that, if chosen properly, they can substantially increase the speed of convergence of the series expansion. This leads to accurate approximate models with only a few coefficients to be estimated. The set of Kautz functions is discussed in detail and, using the power-series equivalence, the truncation error is obtained.

• 한국지반신소재학회논문집
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• 제12권4호
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• pp.87-98
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• 2013

본 연구는 석탄회의 상대밀도를 40%, 60%, 80%, 95%로 각각 변화시켜 등방압축팽창시험과 구속압력을 달리한 배수삼축시험을 하였다. 그리고 이 시험자료를 이용하여 Lade의 단일항복면 구성모델의 토질매개변수의 변화 특성을 알아보았다. 그 결과 탄성성분을 나타내는 토질매개변수 Kur과 n은 상대밀도의 변화에 따른 영향이 미소하고 파괴규준, 경화함수, 소성포텐셜에 관련된 토질매개변수는 상대밀도의 증가에 따라 선형적인 증 감현상을 보이고 있다. 그리고 항복함수에 관련된 토질매개변수 h와 ${\alpha}$는 상대밀도에 따른 변화가 미세하고 파괴규준에 관련한 토질매개변수와 관련성이 매우 높아 파괴규준, ${\eta}_1$에 관한 식으로 대체할 수 있으며, 이 식을 이용하여 수치해석한 결과 양호하게 예측하고 있다.

• Journal of Microbiology and Biotechnology
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• 제28권1호
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• pp.40-49
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• 2018

We evaluated the antioxidant activity and neuronal cell-protective effect of fucoidan extract from Ecklonia cava (FEC) on hydrogen peroxide ($H_2O_2$)-induced cytotoxicity in PC-12 and MC-IXC cells to assess its protective effect against oxidative stress. Antioxidant activities were examined using the ABTS radical scavenging activity and malondialdehyde-inhibitory effect, and the results showed that FEC had significant antioxidant activity. Intracellular ROS contents and neuronal cell viability were investigated using the DCF-DA assay and MTT reduction assay. FEC also showed remarkable neuronal cell-protective effect compared with vitamin C as a positive control for both $H_2O_2$-treated PC-12 and MC-IXC cells. Based on the neuronal cell-protective effects, mitochondrial function was analyzed in PC-12 cells, and FEC significantly restored mitochondrial damage by increasing the mitochondrial membrane potential (${\Delta}{\Psi}m$) and ATP levels and regulating mitochondrial-mediated proteins (p-AMPK and BAX). Finally, the inhibitory effects against acetylcholinesterase (AChE), which is a critical hydrolyzing enzyme of the neurotransmitter acetylcholine in the cholinergic system, were investigated ($IC_{50}$ value = 1.3 mg/ml) and showed a mixed (competitive and noncompetitive) pattern of inhibition. Our findings suggest that FEC may be used as a potential material for alleviating oxidative stress-induced neuronal damage by regulating mitochondrial function and AChE inhibition.

• 한국건강관리협회지
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• 제4권1호
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• pp.28-38
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• 2006

Background : Phentermine classified by "sympathomimmetic amie", is a stimulant of sympatheic tone But there has been no concrete study which presents the influence of phentermine on autonomic nervous system. Analysis of Heart rate variability is reliable, non-invasive and very useful for evaluating function of autonomic nervous system. We tried to elucidate the influence of phentermine on autonomic nervovs system by heart rate variability. MethodsAmong the 70 candidates who participated in the double-blind case control study whichwas designed in purpose of approving whether- Adipekⓓ is effective for treatment of obesity, 45persons were folled up. From April, 2005 to May 2005, HRV of the candidates who takes phentermine or placebo for 1month, was recorded using BFM-5000ⓓ(medi-core) for 5 minutes in resting state. HRV measures were assessed by time-domain and by frequency- domain analysis. Time domain parameters contain SDNN(Standard Deviation of NN intervals) and RMSSD(Root-Mean-Square of Successive Differences), etc and frequency domain Parameters contain Total Power(TP), Low frequency(LF'0.04-0.15Hz) power. High Frequency(HF:0.15-0.4Hz) power and LF/HF ratio etc. Results: Intakes of phentermine reduce HRV significant1y. SDNN & RMSSD, the main tine domain parameters of HRV, were decreased significantly(P=0.007. 0.016). PSI(Physical Stress Index of Pressure Index) was increase significantly(P=0.002)The main frequency domain parameters(TP, LF & VLF), also decreased significantly. (P=0.024,0.033, 0.015)Conclusion: The result showed that intakes of phentermine reduce heart rate variability and influence on most parameters of HRV. So phentermine not only accelerates sympathetic tone, but also inhibit the balance and function of autonomic nervous system.

• Reproductive and Developmental Biology
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• 제32권2호
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• pp.123-126
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• 2008

The objective of this study was to determine the potential hazardous effects of sorting process by flowcytometry on the quality of boar spermatozoa by flowcytometer. Freshly collected boar semen was diluted and divided into two groups; control none sorted and sorted. Sperms in sorted group were processed with flowcytometer for cell sorting with $100\;{\mu}M$ nozzle under the 20 psi pressure. Measurements on each parameter were made at two time points, 0hr (right after sorting) and 24 hr post sorting. Although there was a tendency of lower viability in sorted group than none sorted control group, the percentage of live cells in control ($75.83{\pm}6.92\;&\;59.53{\pm}10.34$) was not significantly different from sorted ($59.70{\pm}7.37\;&\;43.97{\pm}3.76$) at both 0 and 24 hr post sorting. However, sorted sperm showed significantly lower mitochondrial function compared to the control at both 0 h ($79.37{\pm}3.22\;vs.\;63.50{\pm}10.05$) and 24 hr ($67.27{\pm}3.22$ vs. $46.97{\pm}5.37$) time points (p<0.007). Sperm DNA fragmentation rate was significantly lower in control ($22.0{\pm}7.04$) than that of sorted ($32.27{\pm}7.49$) at 24 hr time point (p<0.0002). Taken together, these data suggested thatsorting process by flowcytometer may have influenced sperm motility rather than viability. Also high speed sperm sorting by flowcytometer has significant effects on DNA fragmentation on elapsed time after sorting.

• 한국지반신소재학회논문집
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• 제10권4호
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• pp.29-36
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• 2011

본 연구는 포천 화강토의 건조단위중량을 $16.67kN/m^3$, $17.26kN/m^3$, $17.65kN/m^3$으로 각각 변화시켜 등방압축-팽창시험과 구속압력을 달리한 일련의 배수삼축시험을 하였다. 그리고 이 시험자료를 이용하여 회귀분석을 근거로 건조단위중량의 변화에 따른 Lade의 단일항복면 구성모델의 토질매개변수의 변화 특성을 알아보았다. 그 결과 탄성성분, 파괴규준, 경화함수, 소성포텐셜에 관련된 토질매개변수는 상대밀도의 증가에 따라 선형적인 증 감현상을 보이고 있다. 그리고 항복함수에 관련된 토질매개변수 h와 ${\alpha}$는 상대밀도에 따른 변화가 미세하고 파괴규준에 관련한 토질매개변수와 관련성이 매우 높아 ${\eta}_1$에 관한 식으로 대체할 수 있으며, 이 식을 이용한 수치해석 결과 양호하게 예측하고 있는 것을 확인 할 수 있었다.

• Parasites, Hosts and Diseases
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• 제53권4호
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• pp.421-430
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• 2015

The parasite Plasmodium falciparum causes severe malaria and is the most dangerous to humans. However, it exhibits resistance to their drugs. Farnesyltransferase has been identified in pathogenic protozoa of the genera Plasmodium and the target of farnesyltransferase includes Ras family. Therefore, the inhibition of farnesyltransferase has been suggested as a new strategy for the treatment of malaria. However, the exact functional mechanism of this agent is still unknown. In addition, the effect of farnesyltransferase inhibitor (FTIs) on mitochondrial level of malaria parasites is not fully understood. In this study, therefore, the effect of a FTI R115777 on the function of mitochondria of P. falciparum was investigated experimentally. As a result, FTI R115777 was found to suppress the infection rate of malaria parasites under in vitro condition. It also reduces the copy number of mtDNA-encoded cytochrome c oxidase III. In addition, the mitochondrial membrane potential (${\Delta}{\Psi}m$) and the green fluorescence intensity of MitoTracker were decreased by FTI R115777. Chloroquine and atovaquone were measured by the mtDNA copy number as mitochondrial non-specific or specific inhibitor, respectively. Chloroquine did not affect the copy number of mtDNA-encoded cytochrome c oxidase III, while atovaquone induced to change the mtDNA copy number. These results suggest that FTI R115777 has strong influence on the mitochondrial function of P. falciparum. It may have therapeutic potential for malaria by targeting the mitochondria of parasites.

• 대한토목학회논문집
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• 제31권1C호
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• pp.11-17
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• 2011

본 연구는 백마강 모래의 상대밀도를 25%, 50%, 80%, 100%로 각각 변화시켜 등방압축-팽창시험과 구속압력을 달리한 일련의 배수삼축시험을 하였다. 그리고 이 시험자료를 이용하여 회귀분석을 근거로 상대밀도의 변화에 따른 Lade의 단일항복면 구성모델의 토질매개변수의 변화 특성을 알아보았다. 그 결과 탄성성분을 나타내는 토질매개변수 Kur과 n은 상대밀도의 변화에 따른 영향이 미소하고 파괴규준, 경화함수, 소성포텐셜에 관련된 토질매개변수는 상대밀도의 증가에 따라 선형적인 증 감현상을 보이고 있다. 그리고 항복함수에 관련된 토질매개변수 h와 ${\alpha}$는 상대밀도에 따른 변화가 미세하고 파괴규준에 관련한 토질매개변수와 관련성이 매우 높아 파괴규준, ${\eta}_1$에 관한 식으로 대체할 수 있으며, 이 식을 이용하여 수치해석 한 결과 양호하게 예측하고 있다.

• 한국표면공학회지
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• 제42권1호
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• pp.26-33
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• 2009

Recently ultra-supercritical steam power plants operate at $1000^{\circ}F$ ($538^{\circ}C$) and 3500 psi (24.1 MPa). Thermal efficiency of power plant will be increased about 2% if steam temperature increases from $1000^{\circ}F$ to $1150^{\circ}F$ ($621^{\circ}C$). In this study valve materials Incoloy901 (IC901) and Inconel718 (IN718) were nitrided to improve the surface hardness and solid lubrication function of the valve materials. The hardness of both IC901 and IN718 increased about two times by ion nitriding. IC901, IN718 and their nitrided specimens were corroded under ultra super-critical condition (USC) of $621^{\circ}C$. and 3600 psi (24.8 MPa) for 2000 hours. Oxidations of both IC901 and IN718 were very small due to the formation of protective oxide layer on the surface. But the corrosion resistance of both nitrided specimens decreased because of the formation of non-protective nitride layer of $Fe_{4}N$, $Fe_{2}N$ and CrN on the surface layer. The hardness of both nitrided IC901 and IN718 at $20{\mu}m$ depth from the surface decreased about 30% and 20% respectively by USC 2000 hours.