• KSBB Journal
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• 제17권3호
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• pp.247-254
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• 2002

토양으로부터 2종류 그리고 해양으로부터 1종류의 원유 분해 미생물을 분리하였고, 이들을 strain A132, strain F722 그리고 strain OM1으로 각각 명명하였다. 이들 미생물은 Acinetobacter sp., Pseudomonas aeruginosa, Acinetobacter calcoaceticus로 각각 동정되었다. Strain Al32, F722의 최적배양 및 분해온도는 $35^{\circ}C$이고, 최적 생장 pH는 각각 8과 9에서 나타났다. 원유를 유일한 탄소 원으로 하여 배양을 하였을 때 원유농도 2.0% (w/v)에서 생장이 높았다. 한편, 해양에서 분리된 strain OM1은 pH 7, 원유농도 3.0% (w/v)에서 생장이 높았다. 원유 분해능 조사에서는 Eleuthera (OMAN) 원유를 2.0% (w/v) 기질로 하였을 때, strain Al32가 5.49g/L.day의 분해능을 나타내었다. 반면, L-Zakum (AFRICA) 원유에서는 strain F722가 1.19 g/L.day의 분해능을 보였다. 등유($nC_9\simnC_{20}$)와 경유 ($nC_{9}\simnC_{28}$)에 대하여 분해특성을 조사한 결과 strain OM1은 배양 7일 후 95, 75%를 각각 분해하였다. Strain F722는 배양10일 후 80%의 분해율을 나타내었다.

• Journal of Microbiology and Biotechnology
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• 제20권8호
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• pp.1230-1239
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• 2010

Five bacterial species, capable of degrading the recalcitrant organic compounds (ROCs) diethyleneglycol monomethylether (DGMME), 1-amino-2-propanol (APOL), 1-methyl-2-pyrrolidinone (NMP), diethyleneglycol monoethylether (DGMEE), tetraethyleneglycol (TEG), and tetrahydrothiophene 1,1-dioxide (sulfolane), were isolated from an enrichment culture. Cupriavidus sp. catabolized $93.5{\pm}1.7$ mg/l of TEG, $99.3{\pm}1.2$ mg/l of DGMME, $96.1{\pm}1.6$ mg/l of APOL, and $99.5{\pm}0.5$ mg/l of NMP in 3 days. Acineobacter sp. catabolized 100 mg/l of DGMME, $99.9{\pm}0.1$ mg/l of NMP, and 100 mg/l of DGMEE in 3 days. Pseudomonas sp.3 catabolized $95.7{\pm}1.2$ mg/l of APOL and $99.8{\pm}0.3$ mg/l of NMP. Paracoccus sp. catabolized $98.3{\pm}0.6$ mg/l of DGMME and $98.3{\pm}1.0$ mg/l of DGMEE in 3 days. A maximum $43{\pm}2.0$ mg/l of sulfolane was catabolized by Paracoccus sp. in 3 days. When a mixed culture composed of the five bacterial species was applied to real wastewater containing DGMME, APOL, NMP, DGMEE, or TEG, 92~99% of each individual ROC was catabolized within 3 days. However, at least 9 days were required for the complete mineralization of sulfolane. Bacterial community diversity, analyzed on the basis of the TGGE pattern of 16S rDNA extracted from viable cells, was found to be significantly reduced in a conventional bioreactor after 6 days of incubation. However, biodiversity was maintained after 12 days of incubation in an electrochemical bioreactor. In conclusion, the electrochemical reduction reaction enhanced the diversity of the bacterial community and actively catabolized sulfolane.

• 한국환경농학회지
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• 제30권4호
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• pp.424-431
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• 2011

본 연구에서 우리는 광양만의 해수와 표층 퇴적물에서 TBTCl 내성세균의 개체수를 조사하였다. 광양만에서 TBTCl 내성세균의 개체수는 해수에서 $2.5{\times}10^3-3.8{\times}10^3$ cfu/mL 범위였으며, 표층 퇴적물에서 TBTCl 내성세균의 개체수는 $3.2{\times}10^5-9.1{\times}10^5$ cfu/g 범위였다. 광양만에서 TBTCl 내성세균의 종조성은 Vibrio spp. (19.2%)가 가장 높은 우점종으로 나타났고, Bacillus spp. (16.2%), Aeromonas spp. (15.2%), Pseudomonas spp. (13.1%), Klebsiella spp. (11.1%), Alteromonas spp. (9.1%), Pantoea spp. (6.1%), Proteus spp. (3.0%), Listeria spp. (2.0%), unidentified (5.0%)의 순으로 우점하였다. 또한 11개의 대표적인 TBTCl 내성균주는 여러 중금속들(Cd, Cu, Hg 및 Zn)에도 내성을 나타내었다. 이들 중에서 가장 강한 TBTCl 내성을 보이는 T7 균주를 선별하여, API 20NE등을 이용하여 본 균주의 형태학적, 생리학적 및 생화학적 특성들을 조사하였다. T7 균주는 16S rRNA gene 염기서열 분석에 의해 Pantoea 속으로 동정되어 Pantoea sp. T7으로 명명되었다. 또한 본 균주는 $500{\mu}M$의 TBTCl 농도에서도 60시간 배양 후에 정상 균주 성장의 50.7%까지 증식하였다. Pantoea sp. T7의 생물학적 TBTCl 분해활성은 GC-FPD 분석에 의해 측정되었는데, $100{\mu}M$의 TBTCl 농도에서 배양 40시간 후에 TBTCl 제거 효율은 62.7%로 나타났다.

• 미생물학회지
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• 제28권1호
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• pp.41-46
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• 1990

자연환경으로부터 분리한 DJ-12 균주는 4CBA 및 4CB를 비롯하여 그 대사산물인 4OHBA와 PCA를 분해하여 단일 탄소원으로 이용하였다. DJ-12 균주에서 4CBA 및 4CB분해유전자는 약 65kb 크기의 plasmid인 pDJ121에 존재하였으며, 이 pDJ121은 ExoRI, HindIII, SalI 그리고 PslI의 절단부위를 각각 9, 11, 10 그리고 19개씩 가지고 있었다. EcoRI으로 처리한 pDJ121 절편을 pKT230에 ligation 시켜 재조합 vector인 pDK450을 만들었으며, 이를 Pseudomonas putida KT2440에 transformation 시켜 얻은 cloned cell 에서는 4CBA 분해유전자가 잘 발현되었다.

• 상하수도학회지
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• 제13권4호
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• pp.45-53
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• 1999

The microbial organisms named "Pseudomonas sp. LK-14" were isolated from farm land and shallow river sediment, activated, augmented and identified; which were using 2,4-D (2,4-Dichlorophenoxyacetic acid) as a sole carbon source and energy source. 2,4-D removal efficiency of LK-14 with 2,4-D sole carbon source (reactor S) were higher than that of Activated Sludge with 2,4-D sole carbon source (reactor A). Dynamic bioligical reaction kinetic parameters (sole carbon source was 2,4-D) obtained from batch reactor experiments were ${\mu}_{max}$ $0.105hr^{-1}$, $K_{s,24D}$ 15.64mg/L, $K_{i,24D}$ $1.94h^{r-1}$, $Y_{24D}$ 0.39 for LK-14 and ${\mu}_{max}$ $0.008hr^{-1}$, $K_{s,24D}$ 26.95mg/L, $K_{i,24D}$ $1.75hr^{-1}$, $Y_{24D}$ 0.10 for Activated Sludge. Using these parameters, we could predict the behaviors of 2,4-D substrate utilized by LK-14 and Activated Sludge in batch reactors. The kinetic parameters are enable to predict the 2,4-D substrate and microbial population behavior entering into wastewater treatment plants by using unsteady states dynamic simulation modeling technique.

• 상하수도학회지
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• 제10권4호
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• pp.119-126
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• 1996

Influence factors and efficiency characteristics for treatment of wastewater containing phenol were studied with using Pseudomonas sp. B3. It took 130 hours to remove phenol, when only activated sludge of terminal disposal palnt of sewage was innoculated in batch culture, but it was required just 36 hours, when bacteria degrading phenol and activated sludge were simultaneously innoculated. If only phenol an carbon source was used, it necessary 36 hours for biodegradation of phenol, while glucose was added to medium, it took 73 hours. It was revealed as excellent effluent and SVI, when the F/M ratio, COD and phenol concentration were 53mg/l and 1.2mg/l, respectively, and optimum F/M ratio was revealed 0.31. The reactor were seriously shocked as reducing hydraulic retention time at constant phenol concentration more than increasing phenol concentration at constant hydraulic retention time, when volumetric loading rate was increased to $0.8kg\;phenol/m^3{\codt}d$ from $1.6kg\;phenol/m^3{\codt}d$. And also the effluent phenol concentration was 34mg/l after starting 12 hours of shocking and reactor was recovered as steady state after 65 hours of changing in the former test. Although the effluent phenol concentration was maximum value with 12mg/l after starting 20 hours of shocking and reactor was recovered as steady state after 54 hours of changing in the later test.

• 대한환경공학회지
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• 제31권1호
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• pp.51-57
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• 2009

역삼투 해수담수화(SWRO)공정의 큰 문제점 중 하나인 biofouling 현상을 초기에 감지하기 위한 센서 개발의 선행 연구로써, 본 연구는 역삼투막에 바이오필름을 형성하는 문제성 있는 박테리아를 센서의 타겟 박테리아로 제시하는 것에 중점을 두었다. 문헌조사와 실제 해수담수화 공정에서 사용된 해수 원수와 오염된 역삼투막에 존재하는 박테리아를 계통발생학적으로 분석한 결과를 토대로 Bacillus sp., Flavobacterium sp., Mycobacterium sp., P. aeruginosa, P. fluorescens, 그리고 Rhodobacter sp.의 여섯종의 모델 박테리아를 선정하였고, 선정된 모델 박테리아 중, 막 오염 잠재력을 가진 종을 찾아내기 위해 각각 박테리아의 역삼투막 부착 능력, 고압내성, 그리고 소수성을 비교 분석하였다. 그 결과, 역삼투막 부착능력은 Rhodobacter sp.와 Mycobacterium sp.가 뛰어났으며 소수성이거나 역삼투막(접촉각 약 $63^{\circ}$)과 비슷한 접촉각을 가진 박테리아가 역삼투막에 잘 부착하였다. 800 psi의 고압을 적용 한 후, Rhodobacter sp.는 여섯 종류의 모델 박테리아 중 59-73%의 가장 큰 개체수의 감소를 보였고, P. fluorescens는 1-29%로 가장 높은 고압내성을 보였다. 부착, 소수성, 고압내성 특성을 통한 역삼투막에 biofouling을 유발하는 영향력 있는 박테리아 선정 실험 결과, 여섯 종류의 모델 박테리아 중 Mycobacterium sp.가 부착 능력이 뛰어나고 높은 소수성 특성을 가지며, 800 psi의 고압에서도 50% 이상의 cell의 생물학적 활성능력을 가지고 있어, 막 오염을 유발시키는 가장 잠재력 있는 박테리아로 분석되었다.

• Journal of Microbiology and Biotechnology
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• 제29권1호
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• pp.79-90
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• 2019

Lichens are generally known as self-sufficient, symbiotic life-forms between fungi and algae/cyanobacteria, and they also provide shelter for a wide range of beneficial bacteria. Currently, bacterial-derived biodegradable polyhydroxyalkanoate (PHA) is grabbing the attention of many researchers as a promising alternative to non-degradable plastics. This study was conducted to develop a new method of PHA production using unexplored lichen-associated bacteria, which can simultaneously degrade two ubiquitous industrial toxins, anthracene and naphthalene. Here, 49 lichen-associated bacteria were isolated and tested for PHA synthesis. During the GC-MS analysis, a potential strain of EL19 was found to be a 3-hydroxyhexanoate (3-HHx) accumulator and identified as Pseudomonas sp. based on the 16S rRNA sequencing. GC analysis revealed that EL19 was capable of accumulating 30.62% and 19.63% of 3-HHx from naphthalene and anthracene, respectively, resulting in significant degradation of 98% and 96% of naphthalene and anthracene, respectively, within seven days. Moreover, the highly expressed phaC gene verified the genetic basis of $PHA_{mcl}$ production under nitrogen starvation conditions. Thus, this study strongly supports the hypothesis that lichen-associated bacteria can detoxify naphthalene and anthracene, store energy for extreme conditions, and probably help the associated lichen to live in extreme conditions. So far, this is the first investigation of lichen-associated bacteria that might utilize harmful toxins as feasible supplements and convert anthracene and naphthalene into eco-friendly 3-HHx. Implementation of the developed method would reduce the production cost of $PHA_{mcl}$ while removing harmful waste products from the environment.