Browse > Article

Biodegradation of Crude Oil and Petroleum Products by Crude Oil-degrading Microorganism  

정선용 (전남대학교 환경공학과)
오경택 (전남대학교 환경공학과)
박귀환 (전남보건환경연구원)
이정일 (전남보건환경연구원)
이중기 (전남보건환경연구원)
Publication Information
KSBB Journal / v.17, no.3, 2002 , pp. 247-254 More about this Journal
Abstract
Two kinds of crude oil-degrading microorganisms from soil and one kind from sea were isolated and named strain Al32, strain F722 and strain OM1, respectively. These microorganism were identified Acinetobacter sp., Pseudomonas aeruginosa and Acinetobacter calcoaceticus, respectively. The optimum cultivation temperature of Acinetobacter sp. A132 and P. aeruginosa F722 was $35^{\circ}C$ and optimum growth pH was 8 and 9, respectively. The growth was the highest at 2.0% (w/v) substrate concentration when crude oil was only carbon source. The growth of A. calcoaceticus OM1 isolated from sea was the highest at 3.0% (w/v) of crude oil. In inspection of crude oil degradability, strain Al32 showed 5.49 g/L.day with Eleuthera (OMAN), 2.0% (w/v). P. aeruginosa F722 showed 1.19 g/L g/L.day with L-Zakum (AFRICA). In case of kerosene $nC_9\simnC_{20}$ and diesel $nC_9\simnC_{28}$, A. calcoaceticus OM1 was degraded 95% and 75%, respectively, for 7 days culture, and P. aeruginosa F722 was 80% after 10 days.
Keywords
Acinetobacter sp. Al32; Pseudomonas aeruginosa F722; Acinetobacter calcoaceticus OM1; degradability;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Swannell, R P. J., K. Lee, and M. McDonagh (1996), Field Evaluations of Marine Oil Spill Bioremediation, Microbiol. Rev. 6, 342-365
2 Haines, J. R, B. A. Wrenn, E. L. Holder, K. L. Strohmeier, R T. Herrington, and A. D. Venosa (1996), Measurement of hydrocarbon-degrading microbial populations by 96-well plate most-probable-number procedure, J. Ind. Microbiol. 16, 36-41   DOI
3 Rho, S, C., C. H. Lee, and D, J. Jahng (1999), Nutritional Factors Affecting Efficiency of a Bioremediation Process for Diesel-Contaminated Soil, Kor. J, Biotechnol. Bioeng. 14, 503-510   과학기술학회마을
4 Wang, Z., M. Fingas and G. Sergy (1994), Study of 22-Year-Old Arrow Oil Samples Using Biomarker Compounds by $GC/MS$, Environ. Sci. Technol. 28, 1733-1746   DOI   ScienceOn
5 Cha, J. Y., S. Y. Chung, Y. S. Cho, Y. L. Choi, B. K. Kim, and Y. C. Lee (1999), Characterization of Crude Oil Degradation by Klebsiella sp. KCL-l Isolated from Sea Water, Kor. J. Appl. Microbiol. Biotechnol. 27, 452-457   과학기술학회마을
6 Huesemann, M. H. (1995), Predictive Model for Estimating the Extent of Petroleum Hydrocarbon Biodegradation in Contaminated Soils, Environ. Sci. Technol. 29, 7-18   DOI   ScienceOn
7 Atlas, R. M. and C. E. Cerniglia (1995), Bioremediation of Petroleum Pollutants Diversity and environmental aspects of hydrocarbon biodegradation, BioSci. 45, 332-338   DOI   ScienceOn
8 Boehm, P. D., D. S. Page, E. S. Gilfiilan, A. E. Bence, W. A. Bums, and P. J. Mankiewicz (1998), Study of the Fates and Effects of the Exxon Valdez Oil Spill on Benthic Sediments in Two Bays in Prince William Sound, Alaska, 1, Study Design, Chemistry, and Source Fingerprinting, Environ. Sci. Technol. 32, 567-576   DOI   ScienceOn
9 Douglas, G. S., A. E. Bence, R. C. Prince, S. J. Mcmillen, and E. I. Butler (1996), Environmental Stability of Selected Petroleum Hydrocarbon Source and Weathering Ratios, Environ. Sci. Technol. 30, 2332-2339   DOI   ScienceOn
10 Foght, J., K. Semple, D. W. S. Westlake, S. Blenkinsopp, G. Sergy, Z. Wang, and M. Fingas (1998), Development of a standard bacterial consortium for laboratory efficacy testing of commercial freshwater oil spill bioremediation agents, J. Ind. Microbiol. Biotech. 21, 322-330   DOI
11 Sidorov, D. G., I. A. Borzenkov, R. R Ibatullin, E. I. Milekhina, I. T. Khramov, S. S. Belyaev, and M. V. Ivanov (1997), A Field Experiment on Decontamination of Oil- polluted Soil Employing Hydrocarbon-oxidizing Microorganisms, Appl. Biochem. Microbiol. 33, 441-445
12 Kim, H, J., B. J. Kim, J. Y. Kong, and H. S, Koo (2000), Isolation and Characterization Oil Degrading Bacteria from Southern Sea of Korea, Kor. J. Biotechnol. Bioeng. 15, 27-34   과학기술학회마을
13 Sin, S. C. (1996), Petroleum and Safety, Petroleum and Lubrication 46-51
14 Ross, D. A. (1995), Introduction to Oceanography, p409, Harper Collins Press, United States
15 http://www.nmpa.go.kr/menu6.htm
16 Huy, N. Q., S. Jin, K. Amada, M. Haruki, N. B. Huu, D. T. Hang, D. T. C. Ha, T. Imanaka, M. Morikawa, and S. Kanaya (1999), Characterization of PetroleumDegrading Bacteria from Oil-Contaminated Sites in Vietnam, J. Biosci. Bioeng. 88, 100-102   DOI   ScienceOn
17 Eve, R R (1992), Bioremediation of Petroleum Contaminated Sites, p121, C. K. Smoley. Press, New York
18 Syoko, K. N., S. Keiji, Y. I. Yukie, T. Haruhisa, V. Kasthuri, Y. Satoshi, T. Hiroki, and H. Shigeaki (1996), Construction of Bacterial Consortia That Degrade Arabian Light Crude Oil, J. Fermen. Bioeng. 82, 570-574   DOI   ScienceOn
19 Sohrabi, M. and A. Mogharei (1999), Some aspects of bioremediation of soil contaminated with petroleum hydrocarbons, Afinidad Lvi. 56, 307-312
20 Atlas, R M. (1996), Principles of Microbiology, 2nd ed., p44, Wm. C. Brown. Press
21 Kapley, A., H. J. Purohit, S. Chhatre, R Shanker, T. Chakrabarti, and P. Khanna (1999), Osmotolerance and hydrocarbon degradation by a genetically engineered microbial consortium, Biore. Tech. 67, 241-245   DOI   ScienceOn
22 Macnaughton, S. J., J. R Stephen, A. D. Venosa, G. A. Davis, Y. J. Chang, and D. C. White (1999), Microbial Population Changes during Bioremediation of an Experimental Oil Spill, Appl. Environ. Microbiol. 65, 3566-3574
23 Oh, K. T., Y. W. Lee, M. Kubo, S. J. Kim, and S. Y. Chung (2000), Isolation, identification and characterization of bacteria degrading crude oil, Kor. J. Soci. Environ. Eng. 22, 1851-1859   과학기술학회마을
24 Venosa, A. D., M. T. Suidan, D. King, and B. A. Wrenn (1997), Use of hopane as a conservative biomarker for monitoring the bioremediation effectiveness of crude oil contaminating a sandy beach, J. Ind. Microbiol. Biotech. 18, 131-139   DOI
25 Sugiura, K., M. Ishihara, T. Shimauchi, and S. Harayama (1997), Physicochemical Properties and Biodegradability of Crude Oil, Environ, Sci. Technol. 31, 45-51   DOI   ScienceOn