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Biodegradation of Crude Oil and Petroleum Products by Crude Oil-degrading Microorganism  

정선용 (전남대학교 환경공학과)
오경택 (전남대학교 환경공학과)
박귀환 (전남보건환경연구원)
이정일 (전남보건환경연구원)
이중기 (전남보건환경연구원)
Publication Information
KSBB Journal / v.17, no.3, 2002 , pp. 247-254 More about this Journal
Abstract
Two kinds of crude oil-degrading microorganisms from soil and one kind from sea were isolated and named strain Al32, strain F722 and strain OM1, respectively. These microorganism were identified Acinetobacter sp., Pseudomonas aeruginosa and Acinetobacter calcoaceticus, respectively. The optimum cultivation temperature of Acinetobacter sp. A132 and P. aeruginosa F722 was $35^{\circ}C$ and optimum growth pH was 8 and 9, respectively. The growth was the highest at 2.0% (w/v) substrate concentration when crude oil was only carbon source. The growth of A. calcoaceticus OM1 isolated from sea was the highest at 3.0% (w/v) of crude oil. In inspection of crude oil degradability, strain Al32 showed 5.49 g/L.day with Eleuthera (OMAN), 2.0% (w/v). P. aeruginosa F722 showed 1.19 g/L g/L.day with L-Zakum (AFRICA). In case of kerosene $nC_9\simnC_{20}$ and diesel $nC_9\simnC_{28}$, A. calcoaceticus OM1 was degraded 95% and 75%, respectively, for 7 days culture, and P. aeruginosa F722 was 80% after 10 days.
Keywords
Acinetobacter sp. Al32; Pseudomonas aeruginosa F722; Acinetobacter calcoaceticus OM1; degradability;
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