• Research in Plant Disease
• /
• v.11 no.1
• /
• pp.80-84
• /
• 2005

Bacterial canker of sweet cherry (Prunus avium L.) was observed in farmers\' orchards in Goesan, Chungbuk in 2003. Typical canker symptoms occurred on the branches or twigs of sweet cherry in early spring and bacterial exudates oozed out of the cracked barks of diseased trees. Watersoaked brown symptoms appeared on the leaves and severe infection caused thorough defoliation on the branches or twigs of sweet cherry. When severely infected branches or twigs were cut, irregular and rusty-colored symptoms in sapwood and heartwood were clearly found, indicating that they can serve as specific symptoms of bacterial canker of sweet cherry. The causal bacterium responsible for the symptoms was isolated purely from the infected sapwood of sweet cherry. Based on its morphological, physiological and biochemical characteristics, the causal bacterium was identified as Pseudomonas syringae pv. morsprunorum. The bacterium was pathogenic on sweet cherry and Japanese apricot, but not on peach, cherry, and kiwifruit. It is proposed that the disease be named as bacterial canker of sweet cherry.

• Microbiology and Biotechnology Letters
• /
• v.37 no.4
• /
• pp.371-376
• /
• 2009

The fruits of sweet cherry are highly appreciated by the consumer due to their precocity and quality, such as their sweetness, color and sourness. In this study, the hot-water extract and its sequential organic solvent fractions were prepared from domestic Napoleon sweet cherry (Prunus avium L.) to investigate antimicrobial and antioxidant activity. The hot-water extract contained about 40% sugars, and the solvent fraction yields for hexane, ethylacetate (EA), butanol, and water residue were 0.01%, 3.45%, 16.30%, and 80.24%, respectively. Contents of total polyphenol and total flavonoid of the fractions were 1.24~5.24%, and 0~3.76%, respectively. Among the fractions, EA fraction showed the highest total polyphenol and total flavonoid concentrations. Evaluation of antimicrobial activity of the extract and fractions revealed that EA fraction and butanol fraction contained strong antibacterial activity against Listeria monocytogenes, Staphylococcus aureus, and Salmonella typhimurium with minimal inhibitory concentration (MIC) of 0.5~1.0 mg/mL. But the extract and fractions tested were not active to Pseudomonas aeruginosa. In a while, only hexane fraction showed anti-Candida activity with 0.5~1.0 mg/mL of MIC. The fraction showed strong activity against different multi-antibiotics resistant strains such as C. albicans CCARM 14020. Antioxidative activity assay showed that EA fraction has a strong DPPH scavenging activity and a reducing power. The $IC_{50}s$ of vitamin E and EA fraction were 15.5 and $195.5\;{\mu}g/mL$, respectively. Our results suggest that the fruit of P. avium L. has high potentials with anti-Candida and antioxidative activity.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2020.08a
• /
• pp.62-62
• /
• 2020

체리나무는 앵두나무아과(Drupaceae) 벚나무속(Prunus) 앵두나무아속에 속하며 유럽 중남부와 소아시아가 원산지이다. 특히 온대지역에 잘 적응하는 체리나무는 재배기간 중 비가 적은 건조한 기후에 적합하다. 최근 국내에서 체리 소비가 급격히 증가함에 따라 체리 수입량[6,454톤('11)→15,855톤('19)]이 꾸준히 증가하고 있는 추세이다. 또한 국내에서 체리나무 재배에 대한 관심이 높아지면서 농경지 및 산지에 식재되어 생산량[289톤('09)→656톤('14)] 및 재배면적[60ha('08)→144ha('14)]도 꾸준히 증가하고 있다. 그러나 체리나무의 산지재배관련 연구는 전무한 실정으로 고품질 체리의 안정적 생산이 가능한 재배기술 개발이 시급히 요구되고 있다. 따라서 본 연구는 산지재배 체리나무의 생육, 결실 및 과실품질에 대한 기초자료를 확보하기 위하여 실시하였다. 공시품종은 '라핀(Lapins)'으로 대목은 '기셀라 6(Gisela 6)'을 사용한 3년과 4년생 체리나무를 조사하였다. 체리나무의 생육특성 조사 결과, 수고 및 근원경은 3년생 체리나무 234.0±23.0 cm, 5.1±0.7 cm로 나타났고 4년생 체리나무는 346.0±27.0 cm, 7.3±0.9 cm으로 조사되었다. 본당 착과수는 3년생 체리나무의 경우 과실이 착과되지 않았고 4년생 체리나무는 563.6±42.9개로 조사되었다. 4년생 체리나무의 과실특성을 조사한 결과, 입중 8.1±0.9 g, 당도 16.3 °Brix, 경도 9.8 N, 산도 1.3%로 조사되었으며, 본당 수확량은 4.6±0.3 kg으로 예측할 수 있었다.

• Korean Journal of Agricultural Science
• /
• v.49 no.4
• /
• pp.821-831
• /
• 2022

American plum line pattern virus (APLPV), a member of the genus Ilarvirus in the family Bromoviridae, is one of the plant quarantine pathogens in Korea. In this study, 15 candidate primer sets were designed and examined to develop a reverse transcription polymerase chain reaction (RT-PCR) assay for plant quarantine inspection of APLPV. Using APLPV-infected and healthy samples, the primer sets were assessed for APLPV detection. To confirm the occurrence of nonspecific reactions, six ilarviruses (Apple mosaic virus, Asparagus virus 2, Blueberry shock virus, Prune dwarf virus, Prunus necrotic ringspot virus, and Tobacco streak virus) and 10 target plants (Prunus mume, P. yedoensis, P. persica, P. armeniaca, P. dulcis, P. tomentosa, P. avium, P. glandulosa, P. salicina, and P. cerasifera) were examined. Finally, two primer sets were selected. These primer sets could generate the expected amplicons even with at least 1 ng of the total RNA template in concentration-dependent amplifications. In addition, a positive clone was developed for use as a positive control in the abovementioned RT-PCR assay.

• The Plant Pathology Journal
• /
• v.28 no.3
• /
• pp.295-298
• /
• 2012

Genetic diversity among Pseudomonas syringae pv. morsprunorum isolates from Prunus mume in Korea and Japan was investigated by comparative sequence analysis of the 16S rRNA gene. The strains included 24 field isolates recovered from P. mume in Korea along with seven Japanese strains. Two strains isolated from P. salicina in Japan, one strain from P. avium in the United Kingdom, and the pathotype strain were also used for comparison with their 16S rRNA gene sequences. Nearly complete 16S rRNA gene sequences were sequenced in all 35 strains, and three sequence types, designated types I, II and III, were identified. Eleven strains consisting of five Korean isolates, five Japanese strains, and one strain from the United Kingdom belonged to type I, whereas the pathotype strain and another 19 Korean isolates belonged to type III. Another four Japanese strains belonged to type II. Type I showed 98.9% sequence homology with type III. Type I and II had only two heterogeneous bases. The 16S rRNA sequence types were correlated with the races of P. syringae pv. morsprunorum. Type I and II strains belonged to race 1, whereas type III isolates were included in race 2. Sequence analyses of the 16S rRNA gene from P. syringae pv. morsprunorum were useful in identifying the races and can further be used for epidemiological surveillance of this pathogen.

• Korean journal of applied entomology
• /
• v.58 no.4
• /
• pp.313-317
• /
• 2019

Stink bugs (Hemiptera: Pentatomidae) are economically important pests of fruit trees in Korea. The aim of the present study was to survey the relative abundance of stink bugs on four stone fruits, maesil (Prunus mume Sieb. et Zucc), cherry (Prunus avium L.), plum (Prunus salicina Lindl), and peach (Prunus persica (L.) Batsch), from 2017 to 2019 in the field. Four stink bug species were observed, including Carbula putoni (Jakovlev), Dolycoris baccarum (L.), Halyomorpha halys (Stål), Plautia stali Scott. H. halys was the most abundant (65%), followed by P. stali (26%), C. putoni (7%), and D. baccarum (2%). H. halys was the dominant species in maesil, cherry, and peach fruits, whereas P. stali was the dominant species in plum fruits. Most (81%) of the stink bugs observed were adults, with nymphs accounting for only 19% of the observed specimens. More stink bugs were observed during the late season than during the early season. These finding suggest that control strategies should be developed for the management of H. halys and P. stali at harvest in stone fruit tree orchards in Korea.

• Journal of Bio-Environment Control
• /
• v.21 no.3
• /
• pp.294-298
• /
• 2012

In sweet cherry (Prunus avium L.) growing there are several severe problem which have to be overcome to produce highly graded fruits because of fruit rots and fruit crackings, if there is frequent precipitation during immature fruit step and picking season. In order to reduce fungicide sprayings and produce qualified fruits in areas with rainy season like as South Korea, rain-sheltered growing is necessary absolutely. Sweet cherry blooms early to medium April in southern area of South Korea. If we depend on honeybees (Apis mellifera) distributed in natural ecosystem, it is not easy to get normal fruit-set every season because of low temperature around blooming time. And also bee keepers seldom sell honeybee hives as a pollinator during spring, instead they keep honeybee hives to get honey. Recently use of B. terrestris as a pollinator of cherry tomato, oriental pumpkin etc. grown in protected cultivation system increase abundantly. Therefore, in this study we studied B. terrestris as an alternate of honeybee to pollinate sweet cherry grown in rain shelter. In part of foraging activity B. terrestris shows staying on a cherry flower for about six second and visiting frequency of 11 flowers per minute. However A. mellifera stayed about 15 second on a flower and visited 4~5 flowers per minute. There were no significant difference in fruit-setting rate and fruit characteristics after using B. terrestris and A. mellifera as pollinators of sweet cherry. Consequently there is no negative effect when we use B. terrestris as an alternate pollinator of A. mellifera in sweet cherry cultivation under rain shelter.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.21 no.1
• /
• pp.5-13
• /
• 2019

The first commercial planting of sweet cherry in Korea is thought to be done in early 1920s. The planting area of sweet cherry in Korea is about 500 ha in 2017. Although sweet cherry is considered a minor fruit in Korea, it is one of premium fruits in market and popular among children and women, especially among pregnant women. The import of sweet cherry fruits has increased dramatically in recent years. Seedlings of Prunus lannesiana or clones of 'Colt' (P. avium × P. pseudocerasus) were usually used as sweet cherry rootstocks in Korea. During recent decades new dwarfing rootstocks for cherry such as 'Gisela' series were developed in Germany. Among them, several 'Gisela' series have been mainly used in international nurseries. In this study, we investigated optimum rooting condition of a dwarfing rootstock 'Gisela 5' in summer season cuttings. Among eight soil conditions tested, saprolite + commercial nursery soil (1:1) and saprolite + vermiculite (1:1) showed higher rooting percentage than others, showing 93.6% and 88.9%, respectively. More than 95% of 'Gisela 5' rootstocks produce roots when it was treated with IBA for several seconds just before cuttings, irrespective of concentrations (500 to 2000 mg·L^-1).

• The Plant Pathology Journal
• /
• v.30 no.2
• /
• pp.178-182
• /
• 2014

Among imported plants, seeds are the items that have many latent pathogens and are difficult to inspect. Also, they are the import and export items whose market is expected to expand. The biggest problem with seeds is viruses. Prune dwarf virus (PDV) is the virus that is commonly inspected in Prunus cerasifera, P. persica, P. armeniaca, P. mandshurica, P. cerasus, P. avium or P. serotina seeds. In this study, two RT-PCR primer sets, which can promptly and specifically diagnose plant quarantine seed-transmitted PDV, were developed; and nested PCR primers, where products amplify 739 and 673 nucleotides (nt), and an nested PCR-product, 305 nt, can be obtained as these products are amplified again, were developed. Also, a modified-positive control plasmid was developed, where the restriction enzyme XhoI, which can identify the contamination of samples from the control, was inserted. The method developed in this study has detected PDV in 18 cases since 2007, and is expected to continuously contribute to the plant quarantine in Korea.

• Food Science and Preservation
• /
• v.17 no.3
• /
• pp.370-375
• /
• 2010

Cherries (Prunus avium L) were selected at the light red (LR) and dark red (DR) stages of maturation. Soluble solid content and the physicochemical properties of fresh 'Bing' cherries were analyzed to identify an instrumental nondestructive attribute reflecting changes in sweetness. Soluble solid content was significantly correlated with various physicochemical properties (firmness, color, and acid level) in LR-stage cherries. In DR-stage cherries, only firmness was positively correlated with soluble solid content. A positive correlation was found between soluble solid content and firmness of 200 randomly selected cherries. Thus, flesh firmness may be a useful quality factor indicating potential consumer acceptance of 'Bing' cherries.