• Plant Biotechnology Reports
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• 제5권3호
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• pp.245-254
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• 2011

This study describes an efficient protocol for Agrobacterium tumefaciens-mediated transformation of two subgroups of genotype AAA bananas (Musa acuminata cv. Pei Chiao and Musa acuminata cv. Gros Michel). Instead of using suspension cells, cauliflower-like bud clumps, also known as multiple bud clumps (MBC), were induced from sucker buds on MS medium containing $N^6$-Benzylaminopurine (BA), Thidiazuron (TDZ), and Paclobutrazol (PP333). Bud slices were co-cultivated with A. tumefaciens C58C1 or EHA105 that carry a plasmid containing Arabidopsis root-type ferredoxin gene (Atfd3) and a plant ferredoxin-like protein (pflp) gene, respectively. These two strains showed differences in transformation efficiency. The EHA105 strain was more sensitive in Pei Chiao, 51.3% bud slices were pflp-transformed, and 12.6% slices were Atfd3-transformed. Gros Michel was susceptible to C58C1 and the transformation efficiency is 4.4% for pflp and 13.1% for Atfd3. Additionally, gene integration of the putative pflp was confirmed by Southern blot. Resulting from the pathogen inoculation assay, we found that the pflp transgenic banana exhibited resistance to Fusarium oxysporum f. sp. cubense tropical race 4. This protocol is highly advantageous to banana cultivars that have difficulties in setting up suspension cultures for the purpose of quality improvement through genetic transformation. In addition, this protocol would save at least 6 months in obtaining explants for transformation and reduce labor for weekly subculture in embryogenic cell suspension culture systems.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2005년도 추계종합학술대회
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• pp.1031-1034
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• 2005

초기의 웹서비스는 인터넷에 존재하는 응용들을 보다 효율적으로 통합할 수 있는 기술로 인식되어, 지리적으로 분산되어 있는 기업 시스템을 통합하기 위한 목적이나 서로 다른 기업 간의 시스템연동을 위한 목적으로 활용되기 시작하였다. 그러나, 최근에는 웹서비스의 적용이 크게 확산되면서 인터넷의 응용을 넘어 이동통신망 응용까지 확산되고 있는 추세이다. 이는 웹서비스가 아스키 기반의 XML 표준을 기반으로 하고 있어 플랫폼, 프로그래밍 언어, 통신망에 독립적인 특징을 갖으며, 또한 웹의 국제 표준화 기구인 W3C를 중심으로 개발된 표준 기술이기 때문이다. 현재 MS, IBM 등주요 IT 기업들은 이와 같은 웹서비스의 장점을 이용하여 유비쿼터스 환경에 존재하는 다양한 종류의 디바이스를 연동하기 위한 기술에 집중하고 있다. 본 논문에서는 유비쿼터스 환경에서 웹서비스를 적용하기 위해 고려해야 할 기술적 이슈들에 대해서 설명한다.

• 한국정보통신학회논문지
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• 제13권3호
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• pp.546-555
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• 2009

HL7은 병원정보 시스템에서 사용되는 정보와 관련된 표준화된 프로토콜이다. 생체 정보 웹 뷰어 시스템 또한 의료 영상 및 전송에 대해 표준화된 프로토콜이다. 본 논문에서는 ICU(Intensive Care Unit)에 설치된 Central Monitor에서 확인할 수 있는 환자의 생체 정보들을 웹상에서 실시간 확인할 수 있는 시스템 구성을 제안하기 위하여 생체정보 수신 모듈과 웹 뷰어 시스템을 구성하였다. 생체정보 수신 모듈은 Central Monitor로부터 수신할 수 있는 Raw Data 형태의 환자의 생체 정보를 생체 정보 웹 뷰어 시스템에서 사용가능 한 데이터로 분석할 수 있도록 설계가 이루어져 있다. 웹 뷰어 시스템은 환자 생체 정보에 대한 실시간성과 생체 정보에 대한 데이터의 연속성을 부여하여 베드 사이트의 환자에게서 발생된 모든 생체정보에 의해 환자 관리를 데이터 베이스를 이용하여 전산화함으로써 환자의 광범위한 자료 검색이 이루어지므로 획기적인 원격 진료로 활용이 가능하다. 또한 적절한 생체 정보데이터의 교환과 정보 관리의 수정을 통해 병원정보 시스템은 모든 병원 관계자의 보다나은 업무처리를 향상시킬 수 있다.

• 대한물리의학회지
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• 제18권3호
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• pp.121-133
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• 2023

PURPOSE: Computerization using ICF as a protocol can enhance the assessment, communication, and decision-making of various disciplines and cultures, individual functions, disabilities and health to promote communication and understanding among various professionals, organizations, and countries. The empirical foundation for these propositions was provided by delineating of six distinct computerization components. METHODS: This study analyzed 14 papers that combined the medical field and information technology to activate the ICF through computerization. From each of these papers, distinctive advantages were extracted to propose six computerization elements. The validity of these computerization elements was examined. These papers encompass various computerization elements, among which core elements were identified. In particular, six common core elements were extracted from these papers and assumed to be strategic computerization components for ICF activation. A heuristic methodology was employed to validate these components, representing IT technology maturity using four determining indices, which were then presented graphically for validation attempts. RESULTS: Four quantified indices were defined: reliability, cost-effectiveness, support and updates, and collaboration. Using these indices, this study identified elements that leverage existing IT technologies and require new development. The possibility of increasing utility was identified by applying computerization to ICF. CONCLUSION: This study examined the strategic elements of utilizing ICF by computerizing it using a protocol concept and discussed its potential for utilization. The potential to enhance the value of information in social, physical, and cultural contexts was presented by integrating various domains and data within the ICF framework.

• 전기전자학회논문지
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• 제18권3호
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• pp.349-355
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• 2014

반도체 IP를 재사용하여 통합 칩을 개발하기 위해서는 반도체 IP 인터페이스의 정확한 이해가 필수적이다. 그러나 이들 인터페이스는 대부분 원래 설계자의 스타일대로 기술되어 있는데다가 기술 방법이 제각각이어서 통합 칩 설계자가 이해하는데 많은 혼란이 따른다. 본 논문에서는 반도체 IP 인터페이스를 기술하는 표준화된 방법을 제안한다. 제안하는 기술 방법은 반도체 IP 인터페이스를 IP 정보, 기술 수준, 모델 제공, 데이터 타입, 인터페이스 정보, 포트 정보, 신호 정보, 프로토콜 정보, 소스 파일의 9개 항목으로 나누어 정의한다. 제안된 방법은 통합 칩 설계자가 반도체 IP의 인터페이스를 이해하고 통합 칩을 구현하는데 도움이 된다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제12권4호
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• pp.1396-1414
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• 2018

With the proliferation of the Internet of Things (IoT) healthcare devices, significant interoperability issue arises where devices use proprietary data transfer protocols. The IHE PCD-01 standard has been suggested for the exchange of healthcare data in ISO/IEEE 11073 PHD data model. However, the PCD-01 is not efficient to be used in the IoT environment. This is because the use of SOAP for PCD-01 may be too complex to be implemented in the resource-constrained IoT healthcare devices. In this paper, we have designed a communication system to implement ISO/IEEE 11073 and IHE PCD-01 integration using the IETF CoAP. More specifically, we have designed the architecture and procedures, using CoAP, to seamlessly transmit the bio-signal from the tiny resource-constrained IoT healthcare devices to the server in a standardized way. We have also built the agent, gateway, and PCD-01 interface at the server, all of which are using the CoAP as a communication protocol. In order to evaluate the performance of the proposed system, we have used the PCD data to be transmitted over CoAP, MQTT, and HTTP. The evaluation of the system performance shows that the use of CoAP results in faster transaction and lesser cost than other protocols, with less battery power consumption.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 추계학술대회
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• pp.579-584
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• 2004

In this paper, we present the mechanical, electrical system design and system integration of controllers including sensory devices of the humanoid, KHR-2 (KAIST Humanoid Robot - 2). We have developed KHR-2 since 2003. Total number of DOF of KHR-2 is 41. Each arm including a hand has 11 DOF and each leg has 6 DOF. Head and trunk also has 6 DOF and 1 DOF respectively. In head, two CCD cameras are used for eye. To control all axes efficiently, distributed control architecture is used to reduce computation burden of main controller and to expand devices easily. So we developed the sub-controller as a servo motor controller and a sensor interfacing devices using microprocessor. The main controller attached its back communicates with sub-controllers in real-time by CAN (Controller Area Network) protocol. We used Windows XP as its OS (Operation System) for fast development of main control program and easy extension of peripheral devices. And RTX HAL extension commercial software is used to realize the real-time control in Windows XP environment.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2003년도 학술회의 논문집 정보 및 제어부문 A
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• pp.163-166
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• 2003

In recent years, there has been increased to access multimedia service through the Internet due to rise of the access speed. We suppose the co-existence of the various access networks in adjacent area in the future, such as wireless LAN, Bluetooth, and 3G Cellular So, the size of the cell will be smaller and the number of access point will be increased. In other words, the potable devices need the efficient mobility scheme due to move between cells frequently Currently, the several of the scheme, which is Cellular IP, HAWAII, Mobile IPv4 regional registration, and hierarchical Mobile IPv6, is suggested to support micro-mobility but Mobile IPv4 is standard scheme to support the mobility of the mobile node. MPLS, which forwards the packet through switching instead of routing using IP address, has begun to deploy in the internet backbone to increase the performance of mobility protocol. The integration of both MPLS and Mobile IP improves the scalability of the Mobile IP data forwarding process by leveraging on the features MPLS which are fast switching and high scalability. Moreover it abbreviates IP-in-IP tunneling between HA and FA. In this paper, we suggest efficient mobility scheme through integration of both MPLS and hierarchical Mobile IPv6.

• Journal of Plant Biotechnology
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• 제6권2호
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• pp.69-75
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• 2004

Optimal protocol for efficient genetic transformation has been defined to aid future strategies of genetic engineering in pigeon pea with agronomically important genes. Transgenic pigeonpea plants were successfully produced through Agrobacterium tumefaciens-mediated genetic transformation method using cotyledonary node explants by employing defined culture media. The explants were co-cultivated with A. tumefaciens strain C-58 harboring the binary plasmid, pCAMBIA-1301 [con-ferring $\beta$-glucuronidase(GUS) activity and resistance to hygromycin] and cultured on selection medium (regeneration medium supplemented with hygromycin) to select putatively transformed shoots. The shoots were then rooted on root induction medium and transferred to pots containing sand and soil mixture in the ratio of 1:1. About 22 putative TO transgenic plants have been produced. Stable expression and integration of the transgenes in the putative transgenics were confirmed by GUS assay, PCR and Southern blot hybridization with a transformation efficiency of over 45%. Stable integration and expression of the marker gene has been confirmed in the TO and T1 transgenics through PCR, and Southern hybridization.

• 한국CDE학회논문집
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• 제7권2호
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• pp.110-120
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• 2002

This paper presents the method that allows easy and rapid integration of legacy resources within the company and between departments. The proposed system can easily construct a distributed environment for collaborative design between departments in the companies. It supports knowledge-based integration system, which allows designers to develop product with deep knowledge about product design. For the purpose, DOME (Distributed Object-based Modeling Environment)-which has been developed through various studies-was used in this paper. To overcome its problems and insufficiency, the Web-Integrator is proposed. The Web-Integrator is very suitable for an Internet environment because it uses HTTP (Hyper Text Transfer Protocol) and XML (extensible Markup Language) as its main communication method. By supporting the remote object access via URL (Uniform Resource Locator), the implementation of the integrated system makes the Web-Integrator systematic and intuitive. All the functions and resources provided by DOME could be used with the interface that enables bi-directional communication with the DOME system. Web-Integrator provides full web-based environments for the general designers, who do not have a full design knowledge and experience, and the proposed system allows design operations to happen at any place and anytime. Also it provides XML-RPC(Remote Procedure Call) based web service framework, which allows other systems to use easily the service that the DOME system supplies regardless the location and the platform.