• Journal of Nutrition and Health
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• v.55 no.5
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• pp.543-557
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• 2022

Purpose: Conditions that limit activity constitute one of the important factors that threaten the health of the elderly. This study sought to analyze the dietary habits and nutritional intake status of the Korean elderly who were faced with activity limitations. Methods: Utilizing data from the 2019 Korea National Health and Nutrition Examination Survey, we classified 1,383 elderly people aged 65 and over into 2 groups: those with activity limitations (n = 222, LG) and those without (n = 1,161, NG). We conducted a comparative analysis of the general characteristics, dietary habits, intake of major food groups, energy, and major nutrients. The nutrient adequacy ratio (NAR), index of nutritional quality (INQ), and healthy eating practices were also analyzed. Complex sampling analysis was utilized for all the analyses with energy intake and socio-demographic variables adjusted. Results: The average age (73.8 years) of members of the LG was higher than those of the NG (72.6 years; p = 0.010). A higher percentage of participants in the LG lived alone (28.1%) compared to those in NG (17.2%; p = 0.004). The LG participants tended to regard their health status as "bad" or "very bad" (62.7%) more often than those in the NG (19.3%; p < 0.001). The LG subjects ate breakfast less frequently (p = 0.054) and ate out significantly less often than the NG subjects (p < 0.001). The LG subjects ate more grain (p = 0.001), fewer seeds (p = 0.014) and vegetables (p = 0.039). Carbohydrate made up a large percentage of their energy intake (p = 0.026). The LG subjects had significantly lower NARs of energy (p = 0.021), protein (p = 0.031), and riboflavin (p = 0.037). The LG participants also had a significantly lower rate of healthy eating practices (22.7%) compared to those in the NG (37.8%; p < 0.001). Conclusion: Activity-limited elderly, despite their need for appropriate dietary management, were poorly nourished. They were likely to live alone, thereby making it hard to provide them with social support. Hence, there is a need to provide nutrition education and policy support for the elderly bearing the burden of limited activity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.6
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• pp.392-399
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• 2000

This study was carried out to investigate the physicochemical characteristics of corn silks. Pollination of corn silks was finished within three days after silking, and elongation of open pollinated corn silks was stopped, but unpollinated silks elongated until eight days after silking. Moisture contents of corn silks were about 92-94% at silking stage, but continuously decreased. these were about 70-75% at 30 days after silking. Chlorophyll b was higher than chlorophyll a in corn silks, and chlorophyll a/b ratios of four hybrids were high in this order : silage ＞ sweet ＞ super sweet ＞ waxy corn. Free sugars in corn silks were mainly fructose, glucose and maltose. Their composition rate was 55% of glucose, 42% of fructose and 4% of maltose. Water soluble solid (Brix %) contents of unpollinated corn silks were ranged from 13.7 to 16.8 Brix % and pollinated corn silks were from 12.6 to 13.7 Brix %. Phytic, oxalic, malic. shikimic, glutaric and acetic acid were detected on corn silks. Phytic, oxalic and glutaric acid were considered as a major organic acids in corn silks. Amino acids in corn silks were high in this order : waxy ＞ silage ＞ sweet ＞sweet corn. Serine, glycine and thereoine were contained more than 10%, and five amino acids such as aspartic, glutaric, arginine, alanine and proline were ranged about 5 to 8%. Methionine and cystine, amino acids containing sulfur were contained only small quantity as about 2.1% and 1.3%, respectively. Acetaldehyde, ethanol, acetone, DMS, isobutylaldehyde, cis-3-hexanol, 3-hexe-1-ol, acetate, trans-2-hexanol and pentanol were detected as the volatile components in corn silks, but acetaldehyde and DMS were major volitiles in silage corn silks, and acetaldehyde, ethanol and DMS were major volitiles in waxy corn silks. The length of corn silks was a positively correlated with organic acids (r=${0.556}^*$), and a negatively correlated with amino acids (r=${-0.514}^*$), respectively. Free sugars were positively correlated with all characteristics tested and significantly correlated with organic acid (r=TEX>${0.703}^{**}$), and flavonoids (r=TEX>${0.544}^*$). Chlorophyll was significantly correlated with flavonoid contents (r=TEX>${0.523}^*$). For this reason chlorophyll was evaluated as an indirect selection character for the high flavonoid containing varieties.

• Journal of Microbiology and Biotechnology
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• v.9 no.1
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• pp.106-112
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• 1999

The therapeutic potential of phosphodiesterase 4(PDE4) inhibitors in inflammatory diseases including some autoimmune diseases has been explored recently with some hopeful results. These PDE4 inhibitors are thought to show their anti-inflammatory effect by down-regulating tumor necrosis factor-a (TNF-$\alpha$) production in lymphocytes and macrophages. A high concentration of TNF-$\alpha$has been found in rheumatoid arthritis (RA) synovium and reducing TNF-$\alpha$using biological agents was proven to be an effective RA treatment. To test the possibility of using PDE4 inhibitors for RA treatment, the effects of a newly synthesized PDE4 inhibitor, DWP205505, on TNF-$\alpha$ and IL-10 production was tested in cells isolated from normal peripheral blood and rheumatoid arthritis synovial fluid. Cytokine production was assayed at the protein level by sandwich enzyme-linked immunosorbent assay (ELISA) and at the mRNA expression level by semi-quantitative RT-PCR. Another PDE4 inhibitor, RP73401, was used for comparison. DWP205505 and RP73401 had no harmful effect on cell viability up to 10 $\mu$M concentration during the 24 h culture period. DWP205505 as well as RP73401 significantly reduced TNF-$\alpha$ secretion from lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (pBMC) and synovial fluid mononuclear cells (SFMC). The effect of DWP205505 or RP73401 treatment on the mRNA expression of TNF-$\alpha$ was also studied in LPS-stimulated PBMC and SFMC. TNF-$\alpha$ mRNA expression was increased by LPS stimulation and both of the PDE4 inhibitors suppressed TNF-$\alpha$ mRNA expression. For interleukin-l0 (IL-l0), a little different results were obtained from PBMC and SFMC; IL-l0 secretion was unaffected by LPS stimulation and only minimally affected by both of the PDE4 inhibitors in PBMC. In unstimulated SFMC, DWP205505 and RP73401 slightly enhanced IL-10 secretion, while they reduced IL-l0 secretion from LPS-stimulated SFMC where IL-l0 secretion was a lot higher than unstimulated SFMC. These results suggest that the newly synthesized PDE4 inhibitor DWP205505 may have anti-rheumatoid arthritis activity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.67 no.4
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• pp.265-273
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• 2022

Waxy corn (Zea mays L.), which contains homozygous mutant alleles for the waxy1 (wx1) gene, is widely consumed as a snack food in Asia. This study evaluated sixteen agronomic characteristics of inbred Korean waxy corn lines to aid development of high-quality waxy corn cultivars. The plant materials studied were 177 inbred waxy corn lines developed by the National Institute of Crop Science, Rural Development Administration, Republic of Korea. For the tested lines, days to tasseling and silking averaged 77.69±2.22 days (with a range of 56-97 days), and 81.12±7.56 days (66-99 days), respectively. Plant length ranged from 88 to 237 cm (averaged 164.88±22.67 cm), ear length averaged 11.75±2.52 cm (5.0-18.5 cm), and ear width averaged 2.94±0.68 cm (1.4-4.5 cm). The number of rows on each ear of corn averaged 12.22±2.22 (7-32 rows) and the kernel number averaged 24.30±4.22 (9-37 kernels) per row. The crude protein content was 12.05±1.53% (8.90-21.80%) and total starch content was 69.27±5.74% (49.5-83.9%). Principal component analysis revealed that ear width, grain length, ear length, days to tasseling, days to silking, percentage of ear setting height, and total starch are features that allow distinction between the 177 waxy inbred corn lines. Hierarchical cluster analysis identified twelve waxy inbred lines that produce tall plants and have a short silking period. These lines may improve yield among quickly growing corn varieties.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.4
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• pp.249-257
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• 2022

This study was conducted to comparison analysis growth characteristics, yield and forage quality of 18silage corn varieties grown in paddy and upland fields. In this condition, days to silking of Sinhwangok was the shortest with 78 days and Kangdaok the longest with 92 days. Moreover it was confirmed that the difference of days to silking at this condition greatly occurred in the late species than in the precocious species. The height and ear height ratio of corn grown in paddy decreased by 5~10% and increase by 10~15% compared to upland, respectively. It was confirmed that the ear ratio of corn which enhances the forage quality was highest in Sinhwangok at paddy and upland and most of the varieties decreased by 10~30% at paddy rather than upland. The length of ear was also reduced by 10~25%. In fresh yield, Dacheongok showed the highest yield at 65,750 kg/ha in upland and 38,830 kg/ha in paddy. Similar to the fresh yield, dry and TDN yield in Dacheongok also showed the highest yield at 36,910 kg/ha, 18,040 kg/ha in upland and 21,670 kg/ha, 14,390 kg/ha in paddy, respectively. As a result of analyzing crude protein and starch from seeds to evaluate the feed value of corn for silage, there was no difference between cultivars grown in this condition. In addition, as a result of analyzing the contents of ADF and NDF using leaves, stems and seeds, the contents of leaves and stems were lower in P3394 and P1543 at the upland and lower in Singwangok and Daanok when cultivated at paddy. Moreover ADF and NDF contents were decreased in some varieties in paddy rather than upland. Therefore, there is no significant difference in the feed value of corn varieties grown in this condition, it is possible to cultivate corn in paddy if normal growth is achieved through drainage management.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.4
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• pp.215-221
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• 2022

This study was carried out to investigate the growth characteristics, yield and chemical compositions of rye according to mixing ratio of chemical fertilize r(CF) and liquid swine manure (LSM) in paddy field cultivation. The experimental design was arranged in a randomized block design with three replications. The manure fertilizer ratio of five treatments were CF 100% + LSM 0% (C), CF 70% + LSM 30% (T1), CF 50% + LSM 50% (T2), CF 30% + LSM 70% (T3), and CF 0% + LSM 100% (T4) of rye. At this time, the application of liquid swine manure was based solely on nitrogen. Plant length did not show significant differences among treatments. Ear length, leaf length and leaf width were the longest in C, T3, and T2, respectively (p<0.05). The stem diameter showed in the order of T4 > T3 > T2 > T1 > C, which was thicker as the LSM application rate increased (p<0.05). Fresh, dry matter and total digestible nutrient (TDN) yield were the highest in T4 (p<0.05), whereas the lowest in C treatment. Crude protein, neutral detergent fiber (NDF), acid detergent fiber (ADF) and crude fiber content were did not show significant difference among treatments. However, compared to C, crude fat and crude ash were significantly higher in T2 and T3, respectively (p<0.05). Total mineral content decreased significantly as the LSM application rate increased (p<0.05). Total free sugar showed high in T3 and T4 with a high LSM ratio, but showed significantly lower in chemical fertilizer treatment (C) and low LSM treatment (T1) (p<0.05). The analysis of all the above results suggests that the application of LSM is very effective, considering the dry matter yield and the contents of free sugar. In addition, LSM may be possible to grow rye without chemical fertilizer.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.4
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• pp.243-248
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• 2022

This study was conducted to evaluate the effect of feeding the whole crop rice silage (WCRS) which partially replaces the imported grass hay crop on the milking performance of Holstein and profitability. Two experimental diets were used according to the traditional practice of dairy farms. The control diet (C) was composed of 13kg/day of mixed hay, 6.8kg/day concentrate, and orchardgrass/bermudagrass hay (1.8kg/day). The treatment diet (T) was composed of 9.6kg/day of mixed hay, 6.8kg/day of concentrate and 2.2kg/day of WCRS. The CP content of the WCRS (4.6%) was lower than orchardgrass (11.3%) and bermudagrass hay (8.4%) due to the harvest being 30days later than the optimum harvest date of rice. The lower intake was observed in T during the entire experiment period which seems to be related to the higher proportion of fiber in rice (p<0.05). No significant difference was found in milk production between C (26.9kg) and T (26.3kg) during the entire experiment period (p>0.05). Also, no difference in milk fat, milk protein, total solids, and milk composition was observed between the two diets. Higher income per head was resulted in T (21,141won) compared to C (21,915 won). These results indicate that the partial replacement of the grass hay by the WCRS can bring the profit to the dairy farm due to the no difference between the control diet and the treatment diet.

• Journal of Applied Biological Chemistry
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• v.65 no.4
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• pp.261-267
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• 2022

The purpose of this study is to investigate the various functionalities of the peels, pulps, and seeds of passion fruit. Proximate composition, mineral contents, phenolic acid contents, total polyphenols, total flavonoids, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activities, reducing power, α-glucosidase, and α-amylase inhibitory activities were measured for each part of passion fruit. Proximate composition analysis of the passion fruit indicated that moisture content contained (4.78-8.20%), carbohydrate (68.33-73.23%), protein (8.78-13.63%), fat (1.19-11.60%), and ash (1.51-8.80%). K, Ca, Na and Fe were the predominant mineral in the peels. P and Mg were the predominant mineral in the pulps. All the antioxidant activities (total polyphenols, total flavonoids, DPPH radical scavenging, ABTS radical scavenging, and reducing power) showed high results in the seeds. α-Glucosidase and α-amylase inhibitory activities IC₅₀ were in the peels (5.59 and 63.16 mg/mL), in the pulps (3.80 and 31.90 mg/mL), and in the seeds (0.06 and 1.02 mg/mL). These results indicated that the pulps, peels, and seeds of passion fruit have value as natural antioxidants with the high quality functional components.

• Korean Journal of Plant Resources
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• v.36 no.1
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• pp.15-25
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• 2023

Myriophyllum spicatum L. has been used as an ornamental in ponds and aquariums, and as a folk remedy for inflammation and pus. Nevertheless, the biological activity and underlying mechanisms of anti-inflammatory effects are unclear. This study is aimed at investigating the antioxidative and anti-inflammatory activities of ethanol extract of Myriophyllum spicatum L. (EMS) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Antioxidant activity of EMS was assessed by radical-scavenging effects on ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. As inflammatory response parameters produced by LPS-stimulated RAW 264.7 cells were quantified to assess the anti-inflammatory activity of EMS. Our results showed that EMS increased FRAP and DPPH radical-scavenging activity. In EMS-treated RAW 264.7 cells, the production of NO, PGE₂, TNF-α and IL-1β was significantly inhibited at the non-cytotoxic concentration. In addition, EMS significantly attenuated LPS-stimulated the toll-like receptor (TLR) 4/myeloid differentiation protein (MyD) 88 signaling pathway, and inhibited nuclear translocation of nuclear factor-kappa B(NF-κB). Positive correlations were noted between anti-inflammatory activity and antioxidant activity. In conclusion, it was indicated that EMS suppresses the transcription of inflammatory factors by inhibiting the TLR4/MyD88/NF-κB signaling pathway, thereby suppressing LPS-stimulated inflammation in RAW 264.7 cells. This study highlights the potential role of EMS against inflammation and associated diseases.

• Korean journal of applied entomology
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• v.61 no.4
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• pp.605-614
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• 2022

Two major problems currently threaten human survival on Earth: climate change and the rapid aging of the population in developed countries. Climate change is a result of the increase in greenhouse gas (GHG) concentrations in the atmosphere due to the increase in the use of fossil fuels owing to economic and transportation development. The rapid increase in the age of the population is a result of the rise in life expectancy due to the development of biomedical science and technology and the improvement of personal hygiene in developed countries. To avoid irreversible global climate change, it is necessary to quickly transition from the current fossil fuel-based economy to a zero-carbon renewable energy-based economy that does not emit GHGs. To achieve this goal, the dairy and livestock industry, which generates the most GHGs in the agricultural sector, must transition to using low-carbon emission production methods while simultaneously increasing consumers' preference for low-carbon diets. Although 77% of currently available arable land globally is used to produce livestock feed, only 37% and 18% of the proteins and calories that humans consume come from dairy and livestock farming and industry. Therefore, using edible insects as a protein source represents a good alternative, as it generates less GHG and reduces water consumption and breeding space while ensuring a higher feed conversion rate than that of livestock. Additionally, utilizing the functionality of medicinal insects, such as silkworms, which have been proven to have certain health enhancement effects, it is possible to develop functional foods that can prevent or delay the onset of currently incurable degenerative diseases that occur more frequently in the elderly. Insects are among the first animals to have appeared on Earth, and regardless of whether humans survive, they will continue to adapt, evolve, and thrive. Therefore, the use of various edible and medicinal insects, including silkworms, in industry will provide an important foundation for human survival and prosperity on Earth in the near future by resolving the current two major problems.