• Title/Summary/Keyword: Protein-based

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Prediction of Carcass Fat, Protein, and Energy Content from Carcass Dry Matter and Specific Gravity of Broilers

  • Wiernusz, C.J.;Park, B.C.;Teeter, R.G.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.1
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    • pp.42-48
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    • 1999
  • Three experiments were conducted to develop and test equations for predicting carcass composition. In the first study using 52 d-old Cobb ${\times}$ Cobb male broilers, twenty four carcasses were selected from 325 processed birds based upon visual appraisal for abdominal fat (low, medium, high) and assayed for specific gravity (SG), dry matter (DM), fat, protein, and ash. In experiment 2, 120 birds were fed rations containing 2 caloric densities (2,880 and $3,200kcal\;ME_n/kg$ diet) and assayed as described above on weeks 2,3,4,5, and 6. Carcass fat was elevated (p < 0.05) with increased caloric density. In both studies predictive variables were significantly correlated with chemically determined carcass fat, protein, and ash contents. Pooled across the 2 studies, data were used to form SG, DM, and or age based equations for predicting carcass composition. Results were tested in experiment 3, where 576 birds reared to 49-d consumed either 2,880, 3,200, or $3,574kcal\;ME_n/kg$ diet while exposed to constant $24^{\circ}C$ or cycling 24 to $35^{\circ}C$ ambient temperatures. Both dietary and environmental effects impacted (p < 0.05) carcass composition. The fat content analyzed chemically was enhanced from 12.4 to 15.7%, and predicted fat was also elevated from 13.4 to 14.8% with increasing caloric density. Heat distress reduced (p < 0.05) analyzed carcass protein (18.9 vs 18.3%) and predicted protein (18.2 vs 17.5%). Predicted equation values for carcass fat, protein, ash, and energy were correlated with the chemically analyzed values at r=0.96, 0.77, 0.86, and 0.79, respectively. Results suggest that prediction equations based on DM and SG may be used to estimate carcass fat, protein, ash, and energy contents of broilers consuming diets that differ in caloric density (2,800 to $3,574kcal\;ME_n/kg$) and for broilers exposed to either constant ($24^{\circ}C$) or cycling high (24 to $35^{\circ}C$) ambient temperatures during 49-d rearing period tested in the present study.

Regiospecific Protein Perturbation on F NMR Shifts and Photoisomerization of Fluororhodopsins. An Interpretation Based on Recent Crystal Structures of Rhodopsin

  • Colmenares, Letica U.;Liu, Robert S.H.
    • Journal of Photoscience
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    • v.10 no.1
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    • pp.81-87
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    • 2003
  • Based on structural information provided by recently reported crystal structures of rhodopsin, we present rationales for the regiospecific protein perturbation on the previously reported $\^$19/F chemical shifts of the vinyl and trifluoromethylrhodopsins and their photoproducts. The crystal structures also suggest that H-bonding is a likely cause for the earlier reported regiospecific photoisomerization of the 10-fluororhodopsins. Photoisomerization was revealed by chemical shift of the photoproducts. Additionally, possible use of 3-bond F,F coupling constants for following photoisomerization of retinal-binding proteins is discussed.

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New Yeast Cell-Based Assay System for Screening Histone Deacetylase 1 Complex Disruptor

  • Jeon, Kwon-Ho;Kim, Min-Jung;Kim, Seung-Young
    • Journal of Microbiology and Biotechnology
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    • v.12 no.2
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    • pp.286-291
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    • 2002
  • Histone deacetylase I (HDAC1) works as one of the components in a nucleosome remodeling (NuRD) complex that consists of several proteins, including metastasis-associated protein 1 (MTA1). Since the protein-protein interaction of HDAC1 and MTA1 would appear to be important for both the integrity and functionality of the HDAC1 complex, the interruption of the HDAC1 and MTA1 interaction may be an efficient way to regulate the biological function of the HDAC1 complex. Based on this idea, a yeast two-hybrid system was constructed with HDAC1 and MTA1 expressing vectors in the DNA binding and activation domains, respectively. To verify the efficiency of the assay system, 3,500 microbial metabolite libraries were tested using the paper disc method, and KB0699 was found to inhibit the HDAC1 and MTA1 interaction without any toxicity to the wild-type yeast. Furthermore, KB0699 blocked the interaction of HDAC1 and MTA1 in an in vitro GST pull down assay and induced morphological changes in B16/BL6 melanoma cells, indicating the interruption of the HDAC1 complex function. Accordingly, these results demonstrated that the yeast assay strain developed in this study could be a valuable tool for the isolation of a HDAC1 complex disruptor.

Enhanced Fluorescence from Silk Protein with TiO2 Scatters (산화티타늄 나노 입자에 의한 실크 단백질 형광 증폭 연구)

  • Rakesh Kumar Jha;Sunghwan Kim
    • Korean Journal of Optics and Photonics
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    • v.35 no.1
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    • pp.30-34
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    • 2024
  • We report a purely protein-based platform for green fluorescence by mixing silk protein with green fluorescence protein, and also report its enhancement by the incorporation of TiO2 nanoparticles. The TiO2 nanoparticles employed have diameters of 100 and 300 nm, with a significant increase in fluorescence (by a factor of 7.5) observed when introducing 300-nm TiO2 nanoparticles. Furthermore, an increase in particle distribution density is found to enhance fluorescence amplification. These research findings suggest that protein-based fluorescent films can be enhanced by the characteristics of nanoparticles, opening up new possibilities in the fields of optics and fluorescence applications.

Treatment of Cow's Milk Protein Allergy

  • Vandenplas, Yvan;De Greef, Elisabeth;Devreker, Thierry
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.17 no.1
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    • pp.1-5
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    • 2014
  • The diagnosis and treatment of cow's milk protein allergy (CMPA) is still a challenge. A systematic literature search was performed using Embase, Medline, The Cochrane Database of Systematic Reviews and Cochrane Central Register of Controlled Clinical Trials for the diagnosis and treatment of cow's milk allergy (CMA). Since none of the symptoms of CMPA is specific and since there is no sensitive diagnostic test (except a challenge test), the diagnosis of CMPA remains difficult. A "symptom-based score" is useful in children with symptoms involving different organ systems. The recommended dietary treatment is an extensive cow milk based hydrolysate. Amino acid based formula is recommended in the most severe cases. However, soy infant formula and hydrolysates from other protein sources (rice) are gaining popularity, as they taste better and are cheaper than the extensive cow's milk based hydrolysates. Recent meta-analyses confirmed the safety of soy and estimate that not more than 10-15% of CMPA-infants become allergic to soy. An accurate diagnosis of CMA is still difficult. The revival of soy and the development of rice hydrolysates challenge the extensive cow's milk based extensive hydrolysates as first option and amino acid formula.

Interaction of a 22 kDa Peptidyl Prolyl cis/trans Isomerase with the Heat Shock Protein DnaK in Vibrio anguillarum

  • Kang, Dong Seop;Moon, Soo Young;Cho, Hwa Jin;Lee, Jong Min;Kong, In-Soo
    • Journal of Microbiology and Biotechnology
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    • v.27 no.3
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    • pp.644-647
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    • 2017
  • Peptidyl prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds preceding prolines. We investigated the protein-protein interaction between a 22 kDa PPIase (VaFKBP22, an FK506-binding protein) and the molecular chaperone DnaK derived from Vibrio anguillarum O1 (VaDnaK) using GST pull-down assays and a bacterial two-hybrid system for in vivo and in vitro studies, respectively. Furthermore, we analyzed the three-dimensional structure of the protein-protein interaction. Based on our results, VaFKBP22 appears to act as a cochaperone of VaDnaK, and contributes to protein folding and stabilization via its peptidyl-prolyl cis/trans isomerization activity.

Prediction of hub genes of Alzheimer's disease using a protein interaction network and functional enrichment analysis

  • Wee, Jia Jin;Kumar, Suresh
    • Genomics & Informatics
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    • v.18 no.4
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    • pp.39.1-39.8
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    • 2020
  • Alzheimer's disease (AD) is a chronic, progressive brain disorder that slowly destroys affected individuals' memory and reasoning faculties, and consequently, their ability to perform the simplest tasks. This study investigated the hub genes of AD. Proteins interact with other proteins and non-protein molecules, and these interactions play an important role in understanding protein function. Computational methods are useful for understanding biological problems, in particular, network analyses of protein-protein interactions. Through a protein network analysis, we identified the following top 10 hub genes associated with AD: PTGER3, C3AR1, NPY, ADCY2, CXCL12, CCR5, MTNR1A, CNR2, GRM2, and CXCL8. Through gene enrichment, it was identified that most gene functions could be classified as integral to the plasma membrane, G-protein coupled receptor activity, and cell communication under gene ontology, as well as involvement in signal transduction pathways. Based on the convergent functional genomics ranking, the prioritized genes were NPY, CXCL12, CCR5, and CNR2.

Effect of exercise on the stability of protein tissues

  • Liu, Weixiao;Liu, Yaorong
    • Advances in nano research
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    • v.13 no.5
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    • pp.487-497
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    • 2022
  • This study investigates the stability of protein tissues regarding the vibration analysis based on the classical beam theory coupled with the nonlocal elasticity theory concerning the exercise impact. As reported in the previous research, four different types of protein tissues are supposed, and the influence of sports training is investigated. The protein tissues are made of protein fibers surrounded by an elastic foundation. The exercise enhances the muscle area and plays an essential role in the stability and strength of protein and muscle tissues. The results are examined in detail to examine the impact of different parameters on the stability of nano protein fibers.

A new purification method for the Fab and F(ab)2 fragment of 145-2C11, hamster anti-mouse CD3ε antibody

  • Kwack, Kyu-Bum
    • BMB Reports
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    • v.33 no.2
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    • pp.188-192
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    • 2000
  • Recombinant protein G has been utilized in the purification of antibodies from various mammalian species based on the interaction of antibodies with protein G. The interaction between immunoglobulin and protein G may not be restricted to the Fc protion of antibodies, as many different $F(ab)_2$ or Fab fragments can also bind to protein G. I found both FAb $F(ab)_2$ of 145-2C11, a hamster anti-mouse $CD3{\varepsilon}$ antibody, bound to the protein G-sepharose. Interestingly, Fab and $F(ab)_2$ of 145-2C11 did not bind to the protein A-sepharose. The binding of Fab and $F(ab)_2$ of 145-2C11 to protein G provided a useful method to remove proteases, chopped fragments of the Fc region, and other contaminating proteins. The remaining intact antibody in the protease reaction mixture can be removed by using a protein A-sepharose, because the Fab and $F(ab)_2$ portions of 145-2C11 did not bind to protein A-sepharose. The specific binding of Fab and $F(ab)_2$ portions of 145-sC11 to a protein G-sepharose (though not to a protein A-sepharose) and binding of intact 145-2C11 to both protein A- and G-sepharose will be useful in developing an effective purification protocol for Fab and $F(ab)_2$ portions of 145-2C11.

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Cell-Based Assay Design for High-Content Screening of Drug Candidates

  • Nierode, Gregory;Kwon, Paul S.;Dordick, Jonathan S.;Kwon, Seok-Joon
    • Journal of Microbiology and Biotechnology
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    • v.26 no.2
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    • pp.213-225
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    • 2016
  • To reduce attrition in drug development, it is crucial to consider the development and implementation of translational phenotypic assays as well as decipher diverse molecular mechanisms of action for new molecular entities. High-throughput fluorescence and confocal microscopes with advanced analysis software have simplified the simultaneous identification and quantification of various cellular processes through what is now referred to as high-content screening (HCS). HCS permits automated identification of modifiers of accessible and biologically relevant targets and can thus be used to detect gene interactions or identify toxic pathways of drug candidates to improve drug discovery and development processes. In this review, we summarize several HCS-compatible, biochemical, and molecular biology-driven assays, including immunohistochemistry, RNAi, reporter gene assay, CRISPR-Cas9 system, and protein-protein interactions to assess a variety of cellular processes, including proliferation, morphological changes, protein expression, localization, post-translational modifications, and protein-protein interactions. These cell-based assay methods can be applied to not only 2D cell culture but also 3D cell culture systems in a high-throughput manner.