• 제목/요약/키워드: Protein Functionality

검색결과 206건 처리시간 0.023초

대나무 추출물의 염색성과 기능성 (제2보) -단백질섬유에 대한 염색성- (Dyeability and Functionality of Bamboo Extracts (Part II) -Dyeing Properties of Protein Fiber-)

  • 정고은;이정순
    • 한국의류학회지
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    • 제35권3호
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    • pp.336-346
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    • 2011
  • Dyeing properties of protein fiber with bamboo colorants were studied by investigating the effect of dyeing conditions such as dye concentration, dyeing temperatures, times on dye uptakes, effect of mordants, and color change. The various colorfastness of dyed fabrics were evaluated for practical use. In addition, the antimicrobial ability, ultraviolet-cut ability, and deodorant ability were estimated. The dye uptake increased as the dyeing concentration increased. Bamboo colorants showed relatively good affinity to protein fiber and produced a yellow color. Dye uptake increased as the dyeing time and temperature increased. Post-mordanting was more effective than pre-mordanting. Mordants, Fe and N.Fe, were effective for increasing dye uptake. The color of fabric mordanted with Cu and N.Cu changed to GY. Colorfastness of dyed fabrics showed a relatively good rating, and mordanting had no significant effect on colorfastness. Dyed silk fabric showed very good antimicrobial abilities of 99.9%. Also, ultraviolet-cut ability and deodorant ability were improved in silk fabric dyed with bamboo extracts.

Improvement of Surface Functionalities, Including Allergenicity Attenuation, of Whole Buckwheat Protein Fraction by Maillard-Type Glycation with Dextran

  • Tazawa, Shigeru;Katayama, Shigeru;Hirabayashi, Masahiro;Yamaguchi, Daiki;Nakamura, Soichiro
    • Preventive Nutrition and Food Science
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    • 제19권4호
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    • pp.327-332
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    • 2014
  • The purpose of the current study was to determine the effects of the introduction of polysaccharide chains onto the molecular surface of buckwheat proteins on buckwheat protein surface functionality. The whole buckwheat protein fraction (WBP) was prepared using 50 mM phosphate buffer (pH 7.5) containing 0.5 M NaCl and covalently linked with 6 kDa, 17.5 kDa, 40 kDa, 70 kDa, or 200 kDa dextran by Maillard-type glycation through controlled dry-heating at $60^{\circ}C$ and 79% relative humidity for two weeks. Conjugation with 40 kDa dextran improved the water solubility and emulsifying properties of WBP without causing a serious loss of available lysine; 84.9% of the free amino groups were conserved. In addition, we found that the introduction of dextran chains onto the molecular surfaces of WBP attenuated the antigenicity of WBP.

어류 알로부터 알칼리 가용화공정을 통해 회수한 Collagenous Protein 획분의 식품 기능특성 (Food Functionality of Collagenous Protein Fractions Recovered from Fish Roe by Alkaline Solubilization)

  • 윤인성;김진수;허민수
    • 한국수산과학회지
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    • 제51권4호
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    • pp.351-361
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    • 2018
  • This study investigated the potential of collagenous protein fractions (CPFs) as functional foods. The specific CPFs studied were recovered from the roe of bastard halibut (BH), Paralichthys olivaceus; skipjack tuna (ST), Katsuwonus pelamis; and yellowfin tuna (YT), Thunnus albacares through the alkaline solubilization process at pH 11 and 12. The buffer capacity, water-holding capacity and solubility of CPFs with pH-shift treatment were significantly better at alkaline pH (10-12) than at acidic pH (2.0). At pH-shift treatment (pH 2 and 12), the foaming capacities of CPFs from ST and YT were improved compared to those of controls, but they were unstable compared to BH CPFs. The emulsifying activity index (EAI, $m^2/g$ protein) of CPFs (controls) was 16.0-21.1 for BH, 20.1-23.9 for ST and 9.3-13.7 for YT (P<0.05). CPFs adjusted to pH 12 showed improved EAI and YT CPFs showed significantly greater emulsifying ability than those from BH and ST. CPFs recovered from fish roe are not only protein sources but also have a wide range of food functionalities, confirming the high availability of fish sausage and surimi-based products as protein or reinforcing materials for functional foods and alternative raw materials.

불레기말(Colpomenia sinuosa)의 최종당화산물 저해 효능 및 신장 보호 효과 (Anti-glycation effect and renal protective activity of Colpomenia sinuosa extracts against advanced glycation end-products (AGEs))

  • 김민경;조치흥;김세라;최인욱;이상훈
    • 한국해양바이오학회지
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    • 제13권2호
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    • pp.94-103
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    • 2021
  • Here, we evaluated the anti-glycation effects and renal protective properties of 70% (v/v) ethanolic extract of Colpomenia sinuosa (CSE) against AGEs -induced oxidative stress and apoptosis at different concentrations (1, 5, and 20 ㎍/mL). At 20 ㎍/mL, CSE showed that anti-glycation activities via the inhibition of AGE formation (51.1%), inhibition of AGEs-protein cross-linking (61.7%), and breaking of AGEs-protein cross-links (33.3%), were significantly (###p < 0.001 vs. non-treated group) lower than the nontreated group. Methylglyoxal (MGO) significantly (***p < 0.001) reduced cell viability (24.4%) and increased reactive oxygen species (ROS) level (642.3%), MGO accumulation (119.4 ㎍/mL), and apoptosis (55.0%) in mesangial cells compared to the nontreated group. Pretreatment with CSE significantly (###p < 0.001) increased cell viability (57.8%) and decreased intracellular ROS (96.5%), MGO accumulation (80.0 ㎍/mL), and apoptosis (22.6%) at 20 ㎍/mL. Additionally, we confirmed intracellular AGEs reduction by CSE pretreatment. Consequently, our results suggest that CSE is a good source of natural therapeutics for managing diabetic complications by the antiglycation effect and renal protective activity against MGO-induced oxidative stress.

가열-건조처리 넙치(Paralichthys olivaceus) 알 농축물의 식품기능성 및 생리활성 (Food Functionality and In Vitro Bioactivity of Olive Flounder Paralichthys olivaceus Roe Concentrates Prepared by Cook-dried Process)

  • 윤인성;강상인;김진수;권인상;김형준;허민수
    • 한국수산과학회지
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    • 제56권1호
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    • pp.7-20
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    • 2023
  • Boil-dried concentrate (BDC) and steam-dried concentrate (SDC) were prepared from olive flounder Paralichthys olivaceus roe using the cook-dried process, and their food functionality and in vitro bioactivity were examined. The buffer capacity of BDC and SDC was found to be stronger in the alkaline region than in the acidic region, and the buffer capacity of SDC was superior to that of BDC. The water holding capacities of these concentrates were 7.6 and 7.4 g/g protein, respectively, both of which were significantly lower than that of freeze-dried concentrate (FDC). The solubility of BDC (13.4%) and SDC (12.7%), foaming capacity of BDC (107.7%) and SDC (110.6%), and oil-in-water emulsifying activity index of BDC (7.7 m2/g) and SDC (9.7 m2/g) were all significantly lower than the corresponding values for FDC (P<0.05). The lower food functionality of BDC and SDC compared with FDC can be attributed to the high-temperature denaturation of proteins during the cook-dried process. The 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical scavenging activities (IC50) of SDC (2.5 mg protein/mL) was 60.4 ㎍/mL, and the angiotensin I converting enzyme inhibitory activity was 80.9%. Olive flounder roe concentrates have good antioxidant and antihypertensive activities, and can be used as materials or ingredients in the processing of seafood and other foods to enhance protein contents and food functionality.

아선약의 염색성과 기능성(제2보) -단백질섬유에 대한 염색성- (Dyeability and Functionality of Catechu(Part II) -Dyeing Properties of Protein Fiber with Catechu-)

  • 남기연;이정순
    • 한국생활과학회지
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    • 제19권4호
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    • pp.709-717
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    • 2010
  • This study investigated the properties and functions of wool and silk dyed with Catechu by examining the effects of dying conditions such as dye concentration, dying temperature, dyeing time, pH level and pre-mordants. These conditions were examined in relation to dye uptake and color changes, washing fastness, light fastness, ultraviolet-cut ability and antimicrobial ability of the dyed fabrics. Catechu showed good affinity to silk fiber. Langmuir adsorption isotherm was obtained, and so it was considered that ionic bondings are formed between Catechu and protein fiber. As the dyeing time and temperature is increased, the dyeability of both silk and wool fabrics also increases. At high temperatures the color of dyed fabrics changes from Y and YR to R. Wool is effective in using Al, Cu, Fe mordant, while silk is effective only in using only Cu mordants. The dyeability was shown to be improved at low pH levels. Additionally, both washing fastness and light fastness were shown to be low. However, the fabric color gradually changed to red was due to mailard reaction of catechol tannin causingby repeated washing and sunlight. The ultraviolet-cut ability was improved for cotton fabric dyed with Catechu. Also, dyed fabric with Catechu showed very good antimicrobial abilities at 99.9%.

Changes in Physicochemical Properties of Soybean Protein due to Acetylation during Incubation with Glucose

  • Kim, Myung-Hee;Kim, Jin-Hee;Kim, Kang-Sung
    • 한국환경보건학회지
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    • 제30권4호
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    • pp.308-313
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    • 2004
  • Native and acetylated soybean protein with acetylation percentage of $25\%$ were incubated with glucose to induce Maillard reaction. Acetylation of ${\varepsilon}$-amino group of lysine residues changed the conformation of soybean protein. The direct uv spectrum of native and acetylated soybean protein showed conformational changes with accessibility of tyrosine and tryptophan residues increased. Acetylation suppressed Maillard reaction between soybean protein and glucose. Acetylated soybean protein showed improved water sorption, fat binding, foam formation, and emulsion activity of the protein, but depressed brown pigment development and trypsin digestion. Thus aceylation prevented deterioration of certain functional characteristics that occurred during storage, besides causing functional characteristics to be improved on its own.

Development of new food protein through chemical modification of rice bran proteins

  • Bae, Dongho;Jang, In Sook
    • Journal of Applied Biological Chemistry
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    • 제42권4호
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    • pp.180-185
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    • 1999
  • Protein concentrate was produced and succinylated from rice bran to assess and improve its functional properties for the purpose of expanding the uses of rice bran proteins. The most effective solvent for the extraction of rice bran proteins was 20% aqueous ethanol at pH 9. The protein content of rice bran protein concentrate produced was 70.0% and the total protein yield was 64.3%. The extent of succinylation of free amino groups in the modified products was 72.8%. Though the modified protein products showed good functional properties including solubility, emulsion properties, and oil absorption capacity, it did not form gel. Succinylation improved solubility and emulsion and gelling properties. These improvements in functionality will enhance the value of rice bran proteins, thus enabling them to be more competitive with other food proteins.

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급이사료의 종류와 냉동저장이 타조육단백질의 기능성에 미치는 영향에 관한 연구 (Evaluation of Diet and Frozen Storage on Protein Functionalities of Ostrich Muscle)

  • 진구복
    • 한국축산식품학회지
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    • 제20권4호
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    • pp.320-325
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    • 2000
  • The diet had an effect(P<0.05) on the nutritional contents of ostrich meat during 4 month of frozen storage. As frozen storage increased up to 4 months, pJ, water holding capacity(WHC) and myofibrillar protein solu-bility($\mu$g/$\mu$l) were reduced (P<0.05), how-ever, increased drip loss(DL, %) was found in ostrich muscle from forage fed ostriches, This study suggests that forage fed ostriches, This study suggests that frozedn storage(-2$0^{\circ}C$)up to 4 months in ostrich FCL muscle (outside strip)could be reduced protein functionality, due to increase in DL. decrease in WHC, and markedly decrease in myofibrillar protein solubility($\mu$g/$\mu$l).

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한외여과에 의해 제조된 참깨박 농축단백질의 기능적 특성 (Functional Properties of Sesame Protein Concentrates Produced by Ultrafiltration)

  • 전정례;박정룡
    • 동아시아식생활학회지
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    • 제10권5호
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    • pp.394-403
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    • 2000
  • The functional properties of sesame protein concentrate(SPC) using different size of ultrafiltration(UF) membranes were examined and compared with those of conventional acid-precipitated sesame protein concentrate. The protein contents of SPC by UF with molecular size of 10K, 30K and look dalton membranes were 84.2%, 82.7%, and 76.4%, respectively, and that of acid-precipitated SPC was 88.7%. The nitrogen solubility of SPC by UF was higher than that of conventional SPC at various pH levels. Especially, it showed three-fold increase at near isoelectric point. However, water absorption capacity and fat absorption capacity of SPC by UF were decreased. For emulsion and foam properties, there were no significant differences between SPC by acid precipitation and SPC by UF method. At various pH levels, SPC by membrane with pore size of 30K dalton showed the highest emulsion properties. The SPC by UF had slightly higher viscosity than defatted sesame flour and SPC by acid precipitation.

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