• Korean Journal of Clinical Laboratory Science
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• v.49 no.3
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• pp.263-270
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• 2017

This study was conducted to investigate the dietary supplementation of fruiting body of Hericium erinaceus (HE) mushroom on lipid profiles of serum and histological changes of the liver in rats with high fat and cholesterol diet. Five-week old female Sprague-Dawley albino rats were divided into three groups of 8 rats each: The normal control diet (NC group), high fat and cholesterol diet (HFC group), and HFC diet supplemented with 5% fruiting powder of Hericium erinaceus (HFC+HE group). In the HFC+HE group, serum total cholesterol, low density lipoprotein, and triglyceride concentrations were significantly reduced compared with the NC group. Body weight gain of those in the HFC+HE group were lower than those in the HFC group; whereas HFC+HE had no effect on the levels of plasma albumin, creatinine, blood urea nitrogen, uric acid, glucose, and total protein. The enzyme activities related to the liver function, such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), and, alkaline phosphatase (ALP), were lower in the NC group than in the HFC group, but without significance. Feeding the mushroom increased the excretion of total lipid and cholesterol. A histopathological analysis showed that the those in the HFC group developed hepatic steatosis, whereas those in the HFC+HE group developed small fat droplet. In conclusion, these results suggest that 5% HE supplementation to HFC diet provided health benefits by acting on lowering atherogenic lipid profile in rats with high fat and cholesterol diet.

• Journal of The Korean Society of Grassland and Forage Science
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• v.5 no.2
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• pp.116-120
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• 1985

This experiment was planned to investigate the effects of delayed sealing on fermentation and digestibility of grass silage. The results are summarized as follows: 1. Internal temperature of roll bale silage during storage was $26-28^{\circ}C$ in control, but in delayed sealing it was up to $55^{\circ}C$ by heating with delaying seal. 2. The lactic acid contents was markedly decreased in delayed sealing. $NH_3-N/T-N$ was tended to increase. In the chemical composition of silage, ADIN/T-N, fiber and lignin contents were increased in delayed sealing. 3. Crude protein digestibility was lowered in delayed sealing as compared with control. Fiber digestibility was higher in delayed sealing than in control. 4. The ruminal pH was lowered in delayed sealing. Difference of $NH_3-N$ concentration between control and delayed sealing was not observed. Concentration of total volatile fatty acids (total VFAs) was lower and proportion of acetic acid of total VFAs was higher in delayed sealing than in control.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.6
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• pp.1762-1768
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• 2004

The Korean formula medicine, Gami-Yukmi-Jihwang-Tang (GYJT) has been used for growing slowly, short of stature, incomplete development, fatigue, weak child, growing pain of child. However, it is still unclear how GYJT has an effect on experimental models. In the present study, the author investigated the immune-enhancing effect of GYJT. Forced swimming test (FST) was performed as a model of activity test in mice and measured blood urea nitrogen (BUN), aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactic dehydrogenase (LDH), glucose (Glc) and total protein (TP) in the serum. GYJT (1, 0.1 and 0.01 g/㎏) were orally administered to mice, once per day for 7 days using a feeding atraumatic needle. After 3 days, on FST, the immobility time was significantly decreased in the GYJT (0.01g/㎏/day)-fed group (120.75±5.71s) in comparison with the saline-fed group (153.80±10.74s). After 7 days, the immobility time was significantly decreased in the GYJT (0.1 and 0.01g/㎏/day)-fed group (125.67±5.36s and 107.67±3.71s) in comparison with the saline-fed group (167.67±12.99s). In addition, the contents of BUN and Glc in the blood serum were significantly decreased and the contents of AST, ALT and LDH were also decreased in the GYJT (1g/㎏/day)-fed group. However, the content of TP was not changed. The present results suggest that GYJT may be useful for the anti-fatigue and immune-enhancing agent. Also, the author investigated the effect of GYJT on the production of cytokines in human T-cell line, MOLT-4 cells. However, GYJT has not affected the production of IFN­γ, IL-2, IL-4. These results suggest that GYJT has immune-enhancing effect but does not affect T cell-mediated production of cytokines in the immune function improvement.

• Korean Journal of Poultry Science
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• v.30 no.3
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• pp.169-175
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• 2003

This experiment was conducted to study the optimum energy feeding regimens fur broiler breeders peaked in winter season with 400 caged hens of Ross strain. Four energy supply regimens which were different in daily energy allotment during laying period were employed for 40 weeks from 24 to 64 weeks of age. All experimental diets were formulated to contain 2,750 kcal ME/kg with adjustments made in total feed allotment to provide the desired energy levels. Total consumption of the feed would provide 20 g of protein, 4 g of calcium and 0.35 g of available phosphorus. There were no difference in hen-day egg production and average egg weight among the regimens of energy supply. Feed, ME and feed cost required per egg or per kg egg were significantly increased as the level of energy allotment increased(p<0.05). It was concluded that the energy supply regimen, which supplied 280 kcal ME per day at the age of 24 weeks and then increased the energy supply up to 400 kcal ME per day at the peak period of 30∼34 weeks of age, was superior in feed, ME and feed cost required per egg or per kg egg without any adverse effect on eg production and egg weight.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2002.11a
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• pp.120-121
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• 2002

Two experiments were conducted to investigate the effect of feeding Monascus Purpures(MP) on the growth performance of broiler chicks and egg qualify in laying hens. In Expt 1, MP were supplemented with 0. 0.5, 1.0, 2.0, 4.0% in basal diets. Weight gain, feed intake, feed conversion were examined for five weeks and blood cholesterol, protein were measured at the end of experiment. In Expt 2, diets supplemented with 0, 0.5, 1.0, 2.0, 3.0% MP were fed for two weeks. Egg qualify and yolk cholesterol were weekly measured. In Expt 1, Basal diets containing CP 21.5, 19%, ME 3,100, 3,200 kcal/kg were fed ad libitum for starting and finsihing period, repectively. In Expt 2, hens diet contained CP 16%, ME 2,800 kca1/kg and fed for two weeks. In Expt 1, weight gain of birds fed 2.0% MP was maximized at five weeks of age. Feed efficiency was not statistically different, but tended to improve in 1.0, 2.0% MP supplemental groups compared with control. Total serum cholesterol tended to be lower in 2.0% MP supplemental group but was not statistically different. In Expt 2, albumen height and Haugh unit of birds fed 1.0 and 2.0% MP were higher than other treatments, but was not statistically different. Yolk cholesterol of hens fed MP diets showed no significance.

• Korean Journal of Poultry Science
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• v.30 no.3
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• pp.203-209
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• 2003

This experiment was conducted to investigate the influence of dietary supplementation of two different types of multiple probiotics in broiler chicks. Four hundred one day old male broiler chicks(Ross ${\times}$ Ross) were raised in the floor pen with five treatments(0, A: 0.10, 0.20%, B: 0.10, 0.20% of probiotics), consisting of 5 replicates for 5 weeks. Weight gain, feed intake and feed conversion were measured weekly, Total Lactobacillus, yeast, E. coli, Salmonella were examined from ileum and cecum at the end of experiment. Sera protein, cholesterol and ND vaccine titer were measured and fecal COD, CO$_2$ and NH$_3$ were detected in 24 hours after collection. Weight gain of chickes fed both types of probiotics increased for the first three weeks and showed significantly higher for the rest two weeks. Feed intake tended to be high in probiotics treatments compared with contro(P<0.05)1. Feed conversion improved significantly in B types of 0.2% probiotics compared with control. Total number of Salmonella was not consistent in ileum among treatments, whereas E. coli tended to be decreased in both types of probiotics compared with control. NH$_3$ and CO$_2$ emission of feces were decreased in both types of probiotics, but it was not statistically different(p>0.05). COD of chicks fed both probiotics was lower than control. There were no significantly different breast meat(P>0.05). Total cholesterol decreased in probiotics groups regardless of it's type. ND antibody vaccine titer was prone to be a small increments.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.4
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• pp.551-559
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• 1996

To examine the effects of dietary fibers on gastrointestinal physiology, rats were fed with diets containing 10% sodium alginate,10% cellulose, or fiber-free diets for 5 weeks. The results obtained were as follows: The chronic consumption of sodium alginate induced a significant decrease in body weight gain and feeding efficiency, but a significant increase in length and weight of small intestine. Fecal bulk and weight were higher in fiber-fed group than fiber-free group. The chronic consumption of dietary fiber induced a significant increase in fecal output, resulting in tile decrease of apparent digestibility of protein and lipid. Pancreatic protease activity was lower in fiber-fed group than fiber-free group, whereas pancreatic amylase and lipase activities were not affected. Scanning electron microscopy(SEM) and light microscopy(LM) studies showed small intestine microvilli with numerous ridges and convolutions and goblet cells in fiber-fed groups. As a result of this study, it is concluded that the chronic consumption of dietary fiber decreases apparent digestibility of nutrients and induces morphological and biochemical adaptation of digestive organs.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.5
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• pp.570-575
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• 2001

Three feeding experiments were conducted to investigate the effects of herbs (Obosan-C or Obosan-O) as an additive in formulated diets on growth and body composition of juvenile abalone (Haliotis discus hannai), Three replicate groups of abalone initially average weighing 206 mg, 827 mg and 432 mg were fed the each one of experimental diet containing $0\%\;or\;1\%$ Obosan for 10 (experiment 1), 7 (experiment 2) and 19 weeks (experiment 3), respectively. In addition, an imported commercial diet for abalone was also employed to compare growth performance of juvenile abalone. Weight gain of abalone fed the diet containing Obosan-O was significantly (P<0.05) higher than that of abalone fed the control diet in the experiment 3. Moisture, crude protein and ash contents of soft whole body were not influenced by dietary herbs (P>0.05), whereas crude lipid content of abalone fed the diets containing Obosan-C was significantly (P<0.05) lower than that of abalone fed the control diet in the experiment 2. The results indicate that Obosan-O as an additive in formulated diet can improve body growth of juvenile abalone.

• Journal of the Korea Organic Resources Recycling Association
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• v.14 no.2
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• pp.91-100
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• 2006

Effects of the supplement of earthworm cast produced from the feeding of organic wastes and earthworm on the productivity and nutritional constituents of functional eggs were investigated. Compared with control experiments, the case supplemented with earthworm cast showed high ratios in egg production, selection and the reserved feed. According to the experiment with earthworm, both the number of jumbo eggs and the quantity of reserved feed were increased. Therefore, the nutritional effect of earthworm in the feed was positive. The optimum percentage of earthworm cast in the feed was 10%: the average laying increased to 96.8, which was a 5% increase; the ratio of the large eggs increased by 5% although the ratio of jumbo eggs and of extra large eggs decreased by 5% and 1.1%, respectively; the average reserved feed was 662.5g. Also, Beauveria bassiana was inoculated into the feed as valuable microorganisms to prevent the growth of pathogen and to obtain essential amino acid. With the inoculatio of B. bassiana KACC 40039, the average laying was 0.82/hen and with B. bassiana HYB, it was 0.77/hen. Those numbers were three to eight percentage over the control. As for the effect of inoculation of B. bassiana in the feed on the production of broken eggs, B. bassiana KACC 40039 produced no broken eggs. Analysis of nutritional contents of eggs showed the increase in protein content and decrease in lipid content when compared with the control. According to these results, increase in the income of farmers can be expected.

• Korean Journal of Poultry Science
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• v.13 no.1
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• pp.49-55
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• 1986

In order to study the effects of probiotic and antibiotic on the performance of the chickens and the population of microfloras in the intestine, a feeding trial was conducted for a period of seven weeks. One hundred eighty hatched male chickens of layer attain were fed one of the following three experimental diets; nonmedicated control, antibiotic supplemented diet(virginiamycin 5 ppm) and probiotic supplemented diet. Birds fed probiotic supplemented diets were heavier and consumed more feed than those fed other diets but the differences were not statistically significant. Antibiotic supplemented group showed significantly (P〈0.01) better feed efficiency than other groups. Availabilities of dry matter, crude protein, crude fat, NFE, Ca and P were not significantly different between treatments but antibiotic supplemented group showed significantly(P〈0.05) lower availability of crude fiber than other groups. The weight of small intestine of the birds fed antibiotic supplemented diet was significantly (P〈0.05) lighter than those of other groups. The population of Bacillus subtilis and Lactobacilli in the intestinal content of the birds fed probiotic supplemented diet were significantly (P〈0.01) larger than those of other groups. On the other hand, the population of Coliforms in the probiotic group were significantly (P〈0.01) smaller than those in the nonmedicated control group.