• 한국수소및신에너지학회논문집
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• 제28권5호
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• pp.440-446
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• 2017

This study was aimed at evaluating the effects of carbohydrate, protein and lipid content of substrate on hydrogen yields and microbial communities. The hydrogen yields were linearly correlated to carbohydrate content of substrates while others (content of proteins and lipids) did not make a significant contribution. The chemical composition of substrates produced effects on the final products of anaerobic hydrogen fermentation. Acetate and butyrate were the main fermentation products, with their concentration proving to correlate with carbohydrate and protein content of substrates. The result of microbial community analysis revealed that the relative abundances of Clostridium butyricum increased and Clostridium perfringens decreased as the carbohydrate content increased.

• 한국작물학회지
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• 제1권1호
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• pp.42-45
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• 1963

In a plant breeding program, an efficient selection of desired characters in a population is important. Generally, many agronomic characters in a given population are determined by polygenes and quantitatively inherited. In practice, the genetic relationship between two observed characters which are undoubtedly subjected to the environmental influence is difficult to identify. In recent years, many workers have attempted to understant the genetic relationship between characters in terms of genotypic correlation, and the knowledge thus gained should furnish many important and useful information for the planning of breeding, selection, and interpretation of the result. The genotypic correlation is the result of pleiotropy, linkage of genes(2, 3, 5, 6, 8) and natural or artificial selection(4). The purposes of this study were to estimate genotyric and phenotypic correlations between all possible pairs of nine characters. and to seek certain characters which may be useful as indicators of certain important agronomic characters. Weber and Moorthy(10), Johnson et al. (5) and Sheth(7) found that in general, the genotypic correlations were higher than the phenotypic correlations. Weiss et al. (11) obtained significant positive correlations between maturity and oil content, maturity and low protein content, and high protein content and low oil content. Weber and Moorthy(10) reported the positive genotypic correlations between flowering and maturity, yield and maturity, yield and plant height, yield and seed weight, and negative genotypic correlations between maturity and oil content, and oil content and seed weight. Johnson et al. (5) studied the genotypic and phenotypic correlations among 24 characters and concluded that selection based entirely on a long fruiting period, lateness, heavy seed, low protein, high oil and resistance to lodging would be effective in increasing yield. Sheth(7) found the following positive associations among characters; height and maturity, yield and lodging, low protein content and high oil content, and yield and low protein content. Hanson et al.(1) also reported high negative correlation between seed yield and protein content.

• Asian-Australasian Journal of Animal Sciences
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• 제33권4호
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• pp.556-567
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• 2020

Objective: To improve the utility of native grass resources as feed in China, we investigated the dynamics of protein and carbohydrate fractions among Inner Mongolian native grasses, during ensiling and the aerobic stage, using the Cornell Net Carbohydrate and Protein System. Methods: Silages were prepared without or with lactic acid bacteria (LAB) inoculant. We analyzed the protein and carbohydrate fractions and fermentation quality of silages at 0, 5, 15, 20, 30, and 60 d of ensiling, and the stability at 0.5, 2, 5, and 10 d during the aerobic stage. Results: Inner Mongolian native grass contained 10.8% crude protein (CP) and 3.6% water-soluble carbohydrates (WSC) on a dry matter basis. During ensiling, pH and CP and WSC content decreased (p<0.05), whereas lactic acid and ammonia nitrogen (N) content increased (p<0.05). Non-protein N (PA) content increased significantly, whereas rapidly degraded true protein (PB₁), intermediately degraded true protein (PB₂), total carbohydrate (CHO), sugars (CA), starch (CB₁), and degradable cell wall carbohydrate (CB₂) content decreased during ensiling (p<0.05). At 30 d of ensiling, control and LAB-treated silages were well preserved and had lower pH (<4.2) and ammonia-N content (<0.4 g/kg of fresh matter [FM]) and higher lactic acid content (>1.0% of FM). During the aerobic stage, CP, extract ether, WSC, lactic acid, acetic acid, PB₁, PB₂, true protein degraded slowly (PB₃), CHO, CA, CB₁, and CB₂ content decreased significantly in all silages, whereas pH, ammonia-N, PA, and bound true protein (PC) content increased significantly. Conclusion: Control and LAB-treated silages produced similar results in terms of fermentation quality, aerobic stability, and protein and carbohydrate fractions. Inner Mongolian native grass produced good silage, nutrients were preserved during ensiling and protein and carbohydrate losses largely occurred during the aerobic stage.

• Journal of Plant Biotechnology
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• 제32권2호
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• pp.145-149
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• 2005

본 연구는 나도풍란의 기내 배양에서 몇 가지 종류의 식물생장억제제의 농도별 처리가 식물체 내 유리당 및 단백질 함량에 미치는 영향을 알아보기 위하여 실시되었다. 잎의 유리당의 함량은 생장억제제의 처리 농도가 높아짐에 따라 감소하는 경향이었고, 분석된 유리당 중 glucose의 함량이 가장 높았다. 뿌리의 유리당 함량은 잎과는 반대로 생장억제제 처리 농도가 높아짐에 따라 함량도 같이 높아지는 경향이었으며, 조사된 유리당 중 sucrose의 함량이 비교적 가장 높았다. 잎의 단백질 함량은 생장억제제 처리농도가 높아짐에 따라 감소하는 경향이었으나, 뿌리에서는 증가하는 경향을 나타내었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.268-268
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• 2022

Protein is a major nutrient of food and has long been studied for nutritional and utility value. Among them, rice protein is attracting attention because of its hypoallergenic characteristics and nutritional value. Mutant DM225 with increased protein content was selected by EMS treatment on the weed rice Dharial. QTL analysis of Protein content was carried out using BC₂F₂ populations derived from a cross between "Hanareum2" as a recurrent parent and "DM225" as a donor parent. The protein content of populations was between 5~11%, with an average of 7.7%. To identify QTLs related to Protein content, 117 KASP markers(polymorphic ratio: 15%) showing polymorphisms between the parents were genotyped for the BC₂F₂ population. One QTL was detected between markers SK07 06 and SK07_10 on chromosome 7(LOD: 28.1). This QTL explained 71.4% of the phenotypic variance for Protein content. This QTL will be useful for protein-related rice breeding program.

• 한국작물학회지
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• 제50권4호
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• pp.238-246
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• 2005

Rice yield and protein content have been shown to be highly variable across paddy fields. In order to characterize this spatial variability of rice within a field, two-year experiments were conducted in 2002 and 2003 in a large-scale rice field of $6,600m^2$ In year 2004, an experiment was conducted to know if variable rate treatment (VRT) of N fertilizer, that was prescribed for site-specific management at panicle initiation stage, could reduce spatial variation in yield and protein content of rice while increasing yield compared to conventional uniform N topdressing (UN, 33kg N/ha at PIS) method. VRT nitrogen prescription for each grid was calculated based on the nitrogen (N) uptake (from panicle initiation to harvest) required for target rice protein content of $6.8\%$, natural soil N supply, and recovery of top-dressed N fertilizer. The required N uptake for target rice protein content was calculated from the equations to predict rice yield and protein content from plant growth parameters at panicle initiation stage (PIS) and N uptake from PIS to harvest. This model· equations were developed from the data obtained from the previous two-year experiments. The plant growth parameters for the calculation of the required N were predicted non-destructively by canopy reflectance measurement. Soil N supply for each grid was obtained from the experiment of year 2003, and N recovery was assumed to be $60\%$ according to the previous reports. The prescribed VRT N ranged from 0 to 110kg N/ha with an average of 57kg/ha that was higher than 33 kg/ha of UN. The results showed that VRT application successfully worked not only to reduce spatial variability of rice yield and protein content but also to increase rough rice yield by 960kg/ha. The coefficient of variation (CV) for rice yield and protein content was reduced significantly to $8.1\%$ and $7.1\%$ in VRT from $14.6\%$ and $13.0\%$ in UN, respectively. And also the average protein content of milled rice in VRT showed very similar value of target protein content of $6.8\%$. In conclusion the procedure used in this paper was believed to be reliable and promising method for reducing within-field spatial variability of rice yield and protein content. However, inexpensive, reliable, and fast estimation methods of natural N supply and plant growth and nutrition status should be prepared before this method could be practically used for site-specific crop management in large-scale rice field.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2005년도 국제학술회의
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• pp.57-74
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• 2005

Rice yield and protein content have been shown to be highly variable across paddy fields. In order to characterize this spatial variability of rice within a field, the two-year experiments were conducted in 2002 and 2003 in a large-scale rice field of $6,600m^2$ In year 2004, an experiment was conducted to know if prescribed N for site-specific fertilizer management at panicle initiation stage (VRT) could reduce spatial variation in yield and protein content of rice while increasing yield compared to conventional uniform N topdressing (UN, ,33 kg N/ha at PIS) method. The trial field was subdivided into two parts and each part was subjected to UN and VRT treatment. Each part was schematically divided in $10\times10m$ grids for growth and yield measurement or VRT treatment. VRT nitrogen prescription for each grid was calculated based on the nitrogen (N) uptake (from panicle initiation to harvest) required for target rice protein content of $6.8\%$, natural soil N supply, and recovery of top-dressed N fertilizer. The required N uptake for target rice protein content was calculated from the equations to predict rice yield and protein content from plant growth parameters at panicle initiation stage (PIS) and N uptake from PIS to harvest. This model equations were developed from the data obtained from the previous two-year experiments. The plant growth parameters for this calculation were predicted non-destructively by canopy reflectance measurement. Soil N supply for each grid was obtained from the experiment of year 2003, and N recovery was assumed to be $60\%$ according to the previous reports. The prescribed VRT N ranged from 0 to 110kg N/ha with average of 57kg/ha that was higher than 33kg/ha of UN. The results showed that VRT application successfully worked not only to reduce spatial variability of rice yield and protein content but also to increase rough rice yield by 960kg/ha. The coefficient of variation (CV) for rice yield and protein content was reduced significantly to $8.1\%\;and\;7.1\%$ in VRT from $14.6\%\;and\;13.0\%$ in UN, respectively. And also the average protein content of milled rice in VRT showed very similar value of target protein content of $6.8\%$. Although N use efficiency of VRT compared to UN was not quantified due to lack of no N control treatment, the procedure used in this paper for VRT estimation was believed to be reliable and promising method for managing within-field spatial variability of yield and protein content. The method should be received further study before it could be practically used for site-specific crop management in large-scale rice field.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.325-325
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• 2022

In order to developing high-quality rice varieties and processing varieties, systematic and stable evaluation of physicochemical properties is required for breeding lines. In this study, we compared the quality characteristics evaluation results of rice breeding lines for cooked rice, special rice, and whole crop silage rice adapted to central plain region (Suwon) in order to use as basic data for future rice variety development. Brown rice length/width ratio, head rice ratio, protein content, amylose content, alkali digestion value(ADV) and Toyo value were analyzed to evaluate the quality characteristics of yield trial lines cultivated in Suwon for four years (2017-2020). Brown rice length/width ratio, head rice ratio, protein content, ADV and Toyo values showed significant differences by year, but there was no significant difference in amylose content (p<0.05), which showed little environmental variation. The head rice ratio and Toyo value showed an increasing trend, while the protein content showed a decreasing trend. However, the protein content was the highest in 2020, which is thought to be owing to little sunlight hours due to heavy rainfall in 2020. The protein content of whole crop silage rice was 8.1%, which was significantly higher than that of other lines (p<0.05). Toyo value of medium-maturing and early-maturing lines were 67.6 and 73.7%, respectively, and the Toyo value of medium-maturing lines was higher than that of the early-maturing lines (p<0.05). In correlation analysis among the quality characteristics of the rice lines for cooked rice, significant positive correlations were detected between Toyo value and head rice ratio, amylose content, ADV, and a negative correlation was observed between Toyo value and protein content (p<0.05).

• Asian-Australasian Journal of Animal Sciences
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• 제11권5호
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• pp.545-549
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• 1998

The influence of refeeding with either vitamin, mineral, fibre of water on protein synthesis and mRNA content in the liver and breast muscle of fasted chicks was investigated. At 15 d of age, chicks were fasted for 2 d and then refed either vitamin, mineral, fibre or water. The fractional synthesis rate (FSR) of protein was measured after 30 min of refeeding by using a large dose injection of L - 2, $6[^3H]$ phenylalanine. In the liver, FSR was reduced by fasting and tended to increase but not significantly by refeeding with vitamin or mineral. FSR was not affected by refeeding with fibre or water. There was no influence of fasting and refeeding on ribosomal capacity (the RNA : protein ratio) and ribosomal efficiency (total protein synthesised per total RNA). The absolute synthesis rate (ASR) of liver protein and hepatic mRNA content were reduced by fasting and unchanged by refeeding. In the muscle, FSR, ASR and mRNA content were significantly decreased by fasting and not recovered by refeeding with either vitamin, mineral, fibre or water. It concluded that vitamin, mineral, fibre and water have little capacity to stimulate liver and muscle protein synthesis reduced by fasting.

• 한국작물학회지
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• 제35권5호
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• pp.379-386
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• 1990

우리나라 수도장려품종 1988년 및 '89년산 미와 중ㆍ남부평야지대 6개소(수원, 남양, 이천, 이리, 계화, 밀양)에서 생산된 7개 품종(자포니카 5, 통일형 2)과 중북부 및 남부중산간지와 고랭지 4개소(철원, 진부, 화서, 운봉)에서 생산된 6품종에 대하여 쌀 단백질 함량의 품종 및 환경변이를 분석ㆍ조사하고 단백질 함량에 차이를 보이는 화성벼, 농백 및 용주벼의 세 품종에 대한 아미노산조성을 조사하였던 시험결과를 요약하면 다음과 같다. 1. 우리나라 벼 장려품종의 백미 단백질 함량은 '88년산미는 평균 7.93%에서 5.5-10.2%의 변이, '89년산미는 평균 9.17%에서 6.3-12.0%의 변이로 현저한 년차간 변이를 나타내었고 자포니카 품종군의 평균 단백질 함량 8.22%에 비해 통일형 품종군이 9.3%로 약 1%정도 높았다. 백미 단백질 함량이 높았던 품종은 자포니카에서는 농백, 도봉벼, 치악벼 등이었고, 통일형 품종군에서는 용문벼, 용주벼, 칠성벼 등이었다. 2. 자포니카 저단백 품종인 화성벼는 자로니카 및 통일벼 고단백 품종인 농백 및 용주벼에 비해 필수아미노산 함량의 절대함량은 낮았으나 질소 16.8g당(단백질 100g에 해당) 필수아미노산 함량은 31.7%로 다른 두 고단백 품종과 비슷하였으며 아미노산중에서 화성벼는 arginine, 농백은 alanine과 lysine, 용주벼는 leucine이 가장 높았다. 아미노산중에서 세 품종 모두 글루타민산 함량이 가장 높았고 필수아미노산중에는 leucine이 가장 높았으며 methionine이 가장 낮았다. 3. 쌀 단백질 함량은 한생종일수록 약간 높은 경향을 보였고 백미투명도가 떨어지고 Mg함량이 높을수록 유의하게 높았으며, K/Mg율, 알칼리붕괴도 및 아밀로즈 함량이 낮을수록 높은 경향을 보였는데 이러한 경향은 자포니카 품종군에서 더욱 두드러졌다. 4. 쌀 단백질 함량은 중부평야지산미가 평균 6.74%로 남부평야지산미의 평균 7.54%에 비해 약 1%정도 낮았고 단백질 함량의 품종간 변이와 산지간 변이의 전분산에 대한 기여율이 각각 55.8%와 28.1%로 품종간 변이가 산지간 변이보다 두배정도 컸다. 5. 중북부 중산간지 및 고랭지산미의 쌀 단백질함량은 평균 5.95%로 남부 중산간지 및 고랭지산미의 6.8%보다 약 0.8% 정도 낮았고 단백질 함량의 품종간 변이와 산지간 변이의 전분산에 대한 기여율은 각각 16.7%와 56.4%로 산지간 변이가 품종간 변이보다 약 4배 가까이 컸다.