QTL Mapping for Protein Content Derived from a Cross between oryza sativa and Weedy Rice

  • Ju-Won Kang (Department of Southern Area Crop Science, National Institute of Crop Science, RDA) ;
  • Ji-Yoon Lee (Department of Southern Area Crop Science, National Institute of Crop Science, RDA) ;
  • Gi-Un Seong (Department of Southern Area Crop Science, National Institute of Crop Science, RDA) ;
  • Youngho Kwon (Department of Southern Area Crop Science, National Institute of Crop Science, RDA) ;
  • So-Myeong Lee (Department of Southern Area Crop Science, National Institute of Crop Science, RDA) ;
  • Dong Jin Shin (Department of Southern Area Crop Science, National Institute of Crop Science, RDA) ;
  • Sais-Beul Lee (Department of Southern Area Crop Science, National Institute of Crop Science, RDA) ;
  • Hyunnggon Mang (Department of Southern Area Crop Science, National Institute of Crop Science, RDA) ;
  • Dong Soo Park (Department of Southern Area Crop Science, National Institute of Crop Science, RDA) ;
  • Jong-Hee Lee (Department of Southern Area Crop Science, National Institute of Crop Science, RDA) ;
  • Jun-Hyeon Cho (Sangju Substation, National Institute of Crop Science, Rural Development Administration) ;
  • Gi-Won Oh (Department of Southern Area Crop Science, National Institute of Crop Science, RDA)
  • Published : 2022.10.13

Abstract

Protein is a major nutrient of food and has long been studied for nutritional and utility value. Among them, rice protein is attracting attention because of its hypoallergenic characteristics and nutritional value. Mutant DM225 with increased protein content was selected by EMS treatment on the weed rice Dharial. QTL analysis of Protein content was carried out using BC2F2 populations derived from a cross between "Hanareum2" as a recurrent parent and "DM225" as a donor parent. The protein content of populations was between 5~11%, with an average of 7.7%. To identify QTLs related to Protein content, 117 KASP markers(polymorphic ratio: 15%) showing polymorphisms between the parents were genotyped for the BC2F2 population. One QTL was detected between markers SK07 06 and SK07_10 on chromosome 7(LOD: 28.1). This QTL explained 71.4% of the phenotypic variance for Protein content. This QTL will be useful for protein-related rice breeding program.

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Acknowledgement

This manuscript was supported by Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ014282022022) of the Rural Development Administration, Republic of Korea.