• Archives of Pharmacal Research
• /
• 제13권3호
• /
• pp.285-288
• /
• 1990

The polysaccharide fraction from the rhizome of Trichosanthes kirilowii (Cu-curbitaceae) showed marked antitumor and cytotoxic activity with immunopotentiating activity. It was evidenced by the increase in the number of circulating leucocytes and peritoneal exudate cells and the recovery of lowered antibody forming activity in mice. The polysaccharide was mainly composed of glucose, galactose, fructose, manmose and xylose and a small amount of protein.

• Archives of Pharmacal Research
• /
• 제19권4호
• /
• pp.326-334
• /
• 1996

We investigated the optimum conditions for conversion of water-insoluble components of basidiocarps of Ganoderma lucidum to water-soluble components by hydrolyzing with chitinase. We also tried it with Ganoderma luciclum residue remaining after extracting hot water-soluble components of Ganoderma lucidum. After hydrolyzing under optimum conditions (20 ppm chitinase, 2% Ganoderma lucidum or 6% Ganoderma lucidum residue, at pH 3 and at $ 35^{\circ}C$), the contents of total water-soluble components (polysaccharide or protein) were measured, and it was found that the contents of water-soluble components increased to 1.5-2.7 fold. Michaelis constant, $K_m$ and maximum rate, $V_max$ calculated by Lineweaver-Burk plot for hydrolysis of Ganoderma lucidum were 1.75% and 0.02%/min respectively and those for hydrolysis of Ganoderma lucidum residue were 53.15% and 0.53%/min respectively The protein-bound polysaccharide was isolated after hydrolysis and molecular weights were measured by Sepharose CL-4B gel filtration and compared with the molecular weights of polysaccharide before hydrolysis.

• 약학회지
• /
• 제26권3호
• /
• pp.185-188
• /
• 1982

In order to investigate the antitumor components of Korean wild higher fungi, the carpo-phores of Pholiota squarrosa collected in Kaung Nung area were extracted with water by refluxing for eight hours at 80-90.deg. C. The extracts were dialized for six days against distilled water and lyophilized to produce crude polysaccharide powders. They were found to have the antitumor activity against sarcoma 180 implanted in mice. Especially, the inhibition ratio of the extract of Pholiota squarrosa was 78.7% in the dose of 20mg/kg/day for the period of ten days. The tumor in two of the ten mice was completely regressed. The components of these aqueous extracts were found to be polysaccharide and protein. The total polysaccharide was 42% by Anthrone method and the protein was 55% by Lowry-Folin method in the antitumor component of Pholiota squarrosa.

• 생약학회지
• /
• 제20권2호
• /
• pp.110-116
• /
• 1989

This study was conducted to purify and characterize a polysaccharide fraction from whole parts of Duchesnea indica (Andr.) Focke, which was previously reported to show an antitumor activity by us. The plant was extracted with 5% ethanol for 3 days in the room temperature. The extract was dialyzed by 5% ethanol, and then concentrated by extraction with $excess\;_N-butanol$, followed by gel filtration named Fr. a and b. Each was composed of gallic acid, hexose, pentose, uronic acid and protein, indicating that both fractions were tannic polysaccharide containing protein. Heat treatment of them yielded gallic acid less polysaccharides. They showed a colony stimulating factor activity.

• 한국균학회지
• /
• 제11권2호
• /
• pp.69-77
• /
• 1983

한국산(韓國産) 담자균류(擔子菌類)의 항암(抗癌) 성분(成分)을 탐색하기 위하여, 팽나무버섯의 자실체(字實體)를 온수(溫水)로 추출한 후, visking tube로 투석 및 냉동건조하여 단백성 다당류를 분리하였다. 이 성분을 ICR마우스에 이식한 sarcoma 180에 대한 종양억제작용을 실험하였던 바, 10mg/kg/day 용량(用量)을 10일간 투여한 군(群)에서 그 종양억제율이 대조군에 비하여 62.3%이었다. 처치군의 10마리중 3마리에서 그 종양이 완전히 퇴행하였다. 이 항암성분을 분석하였던 바, 42.4%의 다당류와 24.5%의 단백질을 함유하고 있었다. 그 다당류를 가수분해하여 분석하여 본 바, glucose, galactose, mannose, xylose 및 fucose의 5종의 단당류를 검출하였다. 그 단백질을 가수분해하여 16종의 아미노산을 확인하였다.

• Food Science and Biotechnology
• /
• 제16권4호
• /
• pp.600-604
• /
• 2007

In this study, we first prepared 3 kinds of powders with different molecular masses from the crude protein-bound polysaccharide extract of Agraricus blazei Murill through ultrafiltration, followed by spray-drying. Then, the antitumor activities of the powders were analyzed. Size exclusion chromatography coupled with a multi-angle laser-light-scattering system showed the 3 powders had the following molecular ranges: below 10 kDa (SD-1), 10 to 150 kDa (SD-2), and above 150 kDa (SD-3), representing peak molecular weights of $8.26{\times}10^3,\;9.65{\times}10^4$, and $5.94{\times}10^6\;g/mol$, respectively. All the powders stimulated macrophage RAW264.7 cells to produce nitric oxide, of which SD-2 and SD-3 were superior to the crude extract powder (CP-SD), while SD-1 showed the lowest activity. Similar results were found for their cytotoxicities against human cancer cell lines (A549, MCF-7, and AGS), where the highest activity was obtained with the SD-2 treatment for 72 hr at $1,000\;{\mu}g/mL$. The MCF-7 cell line was less sensitive to the powders than the other cells. From this research we found that ultrafiltration, in combination with spray-drying, is applicable for preparing protein-bound polysaccharide powders with higher antitumor activities.

• 한국식품저장유통학회:학술대회논문집
• /
• 한국식품저장유통학회 2005년도 아시안 푸드의 기능성과 세계화 전략에 관한 국제심포지움
• /
• pp.112-123
• /
• 2005

For thestudying and utilizing the thorn sea cucumber synthetically, the method preparing polysaccharide and polypeptide from sea cucumber was studied. The comparatively rational craft for preparing crude polysaccharide is fresh raw materials deal with oar form, $60\%$ of the ethanol precipitate after hydrolyzed by pretense and vibrated with ultrasonic wave auxiliary. The purification of polysaccharide and removal of protein fromcrude polysaccharide were made through precipitation method using the acetate. The polypeptide is obtained by concentrating in vacuum, freeze-drying after mixing precipitations of two times. Polysaccharide acute poisoning experiment indicate there is no bad reaction when LD50>5000g/kg, there strain rate of liver tumor H11 approach present generally acknowledged tumor treat medicine cyclophosphamide, and there is no side effect. The fatigue resistance function of polypeptide experiment also indicates that the fatigue resistance ability of mouse which fed on added sea cucumber polypeptide has a great improvement.

• 한국식품과학회지
• /
• 제41권3호
• /
• pp.265-271
• /
• 2009

본 연구에서는 냉동반죽의 동결장해와 동결손상으로 제빵성이 저하되는 것을 막기 위한 방법의 하나로 단백질-다당류 혼합물(protein-polysaccharide mixtures, 1:1)을 냉동반죽에 첨가하여 빵을 제조하여 품질 특성을 조사하였다. CA 첨가 빵이 총 부피와 비용적에서 가장 높았고 냉동-해동 cycle별 비용적은 첨가 빵이 대조 빵보다 감소율이 작았다. 빵의 저장중 수분함량의 변화는 첨가 빵이 대조 빵보다 감소율이 작아 수분 보유력에 효과가 있음을 알 수 있었다. Crumb 색도는 첨가 빵이 대조 빵보다 $L^{\ast}$값은 높았으나 $a^{\ast}$와 $b^{\ast}$값은 낮았다. Crust 색도는 CA와 CK 첨가 빵은 $L^{\ast}$, $a^{\ast}$, $^{\ast}b$값 모두 대조 빵보다 낮았지만 WK 첨가 빵은 $^{\ast}L$값이 높았다. 저장 기간중 텍스쳐는 대조 빵과 첨가 빵 모두 견고성과 탄력성이 저장중 증가하였다. 응집성은 저장에 따라 WK 첨가 빵이 대조 빵보다 높았고 씹힘성은 $20^{\circ}C$에서 4일간 저장에서만 대조 빵이 CA, CK, WA 첨가 빵보다는 높았다. 관능 평가 결과, 단백질-다당류 혼합물 첨가 빵이 내부 평가와 외부 평가에서 높은 경향을 보였으나 향, 맛, 전체적인 기호도에서는 유의적인 차이를 보이지 않았다. 위의 결과들을 통해 단백질-다당류 혼합물을 첨가할 경우 제빵 적성을 향상시켜주며 CA와 WA를 냉동반죽 제조 시 첨가하면 동결손상으로 인한 제빵성의 저하를 억제할 수 있으며 빵의 노화를 지연시켜 저장수명을 연장시킬 수 있을 것으로 생각된다.

• 한국식품저장유통학회지
• /
• 제16권4호
• /
• pp.518-524
• /
• 2009

본 연구에서는 냉동반죽의 동결장해.손상으로 제빵성이 저하되는 것을 막기 위한 방법의 하나로 우유단백질과 다당류 혼합물을 냉동반죽에 첨가하여 farinograph와 amylograph를 사용하여 반죽 특성 및 호화 특성 그리고 반죽부피를 조사하였다. Farinogram에 의하면 CA와 CK 첨가구의 수분 흡수율이 대조구보다 증가하였고 반죽 형성시간의 경우에는 CA 첨가구가 대조구의 3배정도 증가하였으며 WA 첨가구도 대조구보다 증가하였다. Amylogram에 의하면 CA, CK 그리고WK 첨가구가 호화개시 온도가 증가하였고, 최고 점도 도달 온도에서도 대조구보다 증가하였으나 최고 점도는 대조구보다 감소하였다. 우유단백질과 다당류 혼합물의 첨가로 반죽의 부피가 증가하는 결과를 가져왔다. 위의 결과들을 통해 단백질 다당류 혼합물을 첨가할 경우 제빵 적성을 향상시켜주며 CA와 WA를 냉동반죽 제조 시 첨가하면 동결손상으로 인한 제빵성의 저하를 억제할 수 있으며 빵의 노화를 지연시켜 저장수명을 연장시킬 수 있을 것으로 생각된다.

• 약학회지
• /
• 제37권5호
• /
• pp.490-498
• /
• 1993

To find antitumor components in the hot water extract from the basidiocarps of Hypsizigus mamomus. protein-bound polysaccharides were purified and fractionated by DEAE-cellulose ion exchange column chromatography and Sepharose CL-4B gel filtration chromatography. When a dose of 20 mg/kg/day was injected intraperitoneally into ICR mice. fraction IV of the component showed the highest inhibition ratio of 73.8% against the count of hemolytic plaque forming cells in mice to 3.2 times. when IV was about 30 KD and the fraction was composed of 76.1% polysaccharide and 4.9% protein. The hexosamine was detected in all the fractions, showing that the polysaccharide and protein moieties were bound each other.