• Bulletin of the Korean Chemical Society
• /
• v.32 no.8
• /
• pp.2732-2736
• /
• 2011

In pH 4.2 HAc-NaAc buffer solution, horseradish peroxidase (HRP) catalyzed $H_2O_2$ oxidation of nanosilver to form $Ag^+$. After centrifugation, $Ag^+$ in the supernatant can be measured by graphite furnace atomic absorption spectrophotometry (GFAAS) at the silver absorption wavelength of 328.1 nm. When HRP concentration increased, the $Ag^+$ concentration in the supernatant increased, and the absorption value enhanced. The HRP concentration in the range of 0.84-50 $ng{\cdot}mL^{-1}$ was linear to the enhanced absorption value (${\Delta}A$), with a regression equation of ${\Delta}A$=0.012C+0.11, correlation coefficient of 0.9988, and detection limit of 0.41 $ng{\cdot}mL^{-1}$ HRP. The proposed GFAAS method was used to detect HRP in waste water samples, with satisfactory results.

• The Plant Pathology Journal
• /
• v.33 no.1
• /
• pp.21-33
• /
• 2017

Citrus blast caused by bacterium Pseudomonas syringae is a very important disease of citrus occuring in many areas of the world, but with few data about genetic structure of the pathogen involved. Considering the above fact, this study reports genetic characterization of 43 P. syringae isolates obtained from plant tissue displaying citrus blast symptoms on mandarin (Citrus reticulata) in Montenegro, using multilocus sequence analysis of gyrB, rpoD, and gap1 gene sequences. Gene sequences from a collection of 54 reference pathotype strains of P. syringae from the Plant Associated and Environmental Microbes Database (PAMDB) was used to establish a genetic relationship with our isolates obtained from mandarin. Phylogenetic analyses of gyrB, rpoD, and gap1 gene sequences showed that P. syringae pv. syringae causes citrus blast in mandarin in Montenegro, and belongs to genomospecies 1. Genetic homogeneity of isolates suggested that the Montenegrian population might be clonal which indicates a possible common source of infection. These findings may assist in further epidemiological studies of this pathogen and for determining mandarin breeding strategies for P. syringae control.

• The Plant Pathology Journal
• /
• v.37 no.6
• /
• pp.512-520
• /
• 2021

In this study, we report genetic characterization of Orobanche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analyses, rbcL gene sequences were analyzed using neigbor joining method and minimum spanning tree. Molecular analyses of RAPD-PCR analysis revealed high genetic diversity of O. cumana populations which indicated high adaptive potential of this parasitic weed in Serbia. Further analyses of rbcL gene using minimum spanning tree revealed clear differences among diverse sections of Orobanche genus. Although this molecular marker lacked the resolution to display intrapopulation diversity it could be a useful tool for understanding the evolution of this parasitic plant. Our results suggested that O. cumana has great genetic potential which can lead to differentiation of more virulent races which is important for determining crop breeding strategies for their control.

• Journal of Korean Society on Water Environment
• /
• v.24 no.5
• /
• pp.592-597
• /
• 2008

Current water quality standard (WQS) in Korea is based on the protection of human health, not considering the protection of aquatic organisms. Most of chemicals can be toxic to ecological biota as well as human. Health of aquatic biota is closely related to the human health via food chain, therefore ecological risk based-WQS needs to be developed to protect the aquatic ecosystem. In this study, we selected the 31 chemicals in the Project entitled 'Development of integrated methodology for evaluation of water environment'. The methodology for calculating water quality criteria was derived from the Australian and New Zealand processes for deriving guideline trigger value for aquatic ecosystem. The available ecotoxicity data were collected from US EPA's ECOTOXicology Database (ECOTOX), TOX-2000 Database, European Chemicals Bureau (ECB)'s International Uniform Chemical Information Database (IUCLID) and Environmental Protection Agency (US EPA)'s report 'Ambient Water Quality Criteria (AWQC)'. The aquatic toxicity data for the Korean species were selected for risk assessment to reflect the Korean water environment. The monitoring values were calculated from the water quality monitoring data four main Korean rivers. We suggested the order of priorities of chemicals based on ecological risk assessment. We expect that these results can be useful information for establishing the WQS for the protection of aquatic ecosystem.

• Journal of Electrochemical Science and Technology
• /
• v.14 no.3
• /
• pp.205-214
• /
• 2023

Soil salinity is becoming one of the most devastating environmental hazards over the years. Soil investigation involves fast, low cost and non disturbing methods to measure soil characteristics for both construction projects as well as for agricultural use. The electrical resistivity of saline soils is greatly governed by salt concentration and the presence of moisture in soil matrix. Experimental results of this investigation highlight that there is a significant relationship between the electrical resistivity of soil samples mixed with chloride solutions (NaCl, KCl, and MgCl₂) at various concentrations, and soil physical properties. Correlations represented by quadratic functions were obtained between electrical resistivity and soil characteristics, namely, water content, degree of saturation and salt concentration. This research reveals that the obtained correlations between electrical resistivity, salt concentration, water content and degree of saturation are effective for predicting the characteristics of salt affected soils in practice, which constitute a governing element in the assessment of saline lands sustaining infrastructure.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2002.04a
• /
• pp.159-162
• /
• 2002

Innovative and nondestructive characterization techniques have been developed to locate and quantify nonaqueous phase liquids (NAPLs) in the vadose and saturated zones in the subsurface environment. One such technique is the partitioning interwell tracer test (PITT). The PITT is a simultaneous displacement of partitioning and non-partitioning tracers through a subsurface formation. Partitioning tracers will partition into the NAPL during their transport through NAPL-contaminated formations. Mean travel times of partitioning and non-partitioning tracers are used to estimate the quantity of NAPL encountered by the displaced tracer pulse. Travel times are directly proportional to the partitioning coefficient and the volume of NAPL contacted in the subsurface environment. This paper discusses the conceptual background, design and implementation of PITTs. (This document has not been subjected to Agency review and therefore does not necessarily reflect the views of the Agency, and no official endorsement should be inferred.)

• Journal of Korean Critical Care Nursing
• /
• v.13 no.1
• /
• pp.15-26
• /
• 2020

Purpose : This study aimed to examine factors affecting radiation protective behaviors among emergency room nurses by assessing knowledge, attitude, and environment for radiation protection. Methods : The study employed a cross-sectional design. Subjects were a convenience sample of 129 nurses working in emergency rooms of three general hospitals. Data were collected using self-report questionnaires and analyzed using t-test, ANOVA, Pearson correlation coefficients, and multiple regression. Results : The more the nurses received radiation safety education (t=2.26, p=.026), used protective gear (t=4.40, p<.001), and took health screenings (t=2.65, p=.009), the higher their levels of radiation protection behavior. There were significant relations between radiation protective behaviors and attitude (r=.27, p=.002), and radiation protective behaviors and environment for radiation protection (r=.55, p<.001). The factors affecting radiation protective behaviors were protective environment (β=.53, p<.001), protective attitude (β=.32, p<.001), and the use of protective gear (β=.24, p=.002). Conclusions : The government, hospital administrators, and radiation protection-related organization should adopt the following measures to protect emergency room nurses from radiation: research and development of shield instrument, medical examination for emergency room nurses, protocol development of radiation protection behaviors, extension of education chances of radiation protection, and encouraging the use of protective equipment.

• Journal of environmental and Sanitary engineering
• /
• v.1 no.1 s.1
• /
• pp.47-58
• /
• 1986

The underground living spaces have become one of the major environment of this decade in urban area. This study was carried out to examine contamination level for purpose of preservation of the pleasant underground environment. Three subway stations and three underground shopping centers in Seoul and two underground shopping centers in Busan were selected and surveyed by measuring gaseous pollutants $(SO_2,\; NO_2,\;CO,\;HC,\; HCHO,\;CO_2)$, dust, airborne microbes, and the other air condtions (temperature, humidity, air pressure, air flow, kata cooling power). These examined data were compared with four kinds of standard (building sanitation management, room air quality standards, occupational safety and health standards, ventilation equipment) as environmental hygiene.

• Journal of Korean Society of Forest Science
• /
• v.111 no.1
• /
• pp.72-80
• /
• 2022

In this study, intention type was assigned to determine appropriate conservation and use of the Baekdudaegan Protection Area. To determine intention type, 84 indices were integrated into 7 types: the Natural Environment Protection District, Natural Scenic District, Agriculture and Forestry Promotion District, Living Environment Protection District, Education/Culture/Arts Promotion District, Recreation/Tourism Promotion District, and Disaster Management District. Intention types were decided by comparing the occupying proportion of the intention types for each watershed segment in which the Seoraksan, Taebaeksan, and Sockrisan region boundaries were included. Weight values were applied to each of the seven intention types because each type did not play an equal role in the target regions. Most regions were classified as Natural Environment Protection intention types because the Baekdudaegan area is regulated with strong forestry and environmental protection acts; thus, the weight values related to nature protection were higher than those for other types. To supplement the results from this study, additional research is needed to determine the intention type and weight for each region, and it will also be necessary to integrate sociocultural factors and field survey data.

• Journal of Radiation Protection and Research
• /
• v.44 no.4
• /
• pp.127-127
• /
• 2019