• 대한의생명과학회지
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• 제14권3호
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• pp.157-165
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• 2008

Recently, the number of patients with prostate cancer has been increased gradually by both the change of living environment and the increase of aged population. In this study the serum prostate specific antigen (PSA) level was compared to the Gleason score known as a prognostic factor for the prostate cancer. In the Gleason score 6 and $9{\sim}10$, the average age was 69.68 years old and 69.52 years old, respectively and there was no statistically difference in both of age and Gleason score. the PSA serum consistency appeared <4 ng/mL as example 1, $4{\sim}20ng/mL$ as example 17 and ${\geq}20ng/mL$ as example 4 in the Gleason score 6, and In the Gleason score $9{\sim}10$, it appeared <4 ng/mL as example 1, $4{\sim}20ng/mL$ as example 6 and ${\geq}20ng/mL$ as example 15. PSA serum consistency in the Gleason score $9{\sim}10$ showed higher value than those of Gleason score 6 (P<0.05). Next, expression ratios of bcl-2, Ki-67 and p53 were examined in the Gleason score 6 and $9{\sim}10$. the p53 expression ratio, a tumor suppression gene, appeared the significance statistically by the classification of the Gleason score as example 7 (28%) in the Gleason score 6 and as example 16 (64%) in the Gleason score $9{\sim}10$ (P<0.05). but not different in the expression ratios of the Ki-67 and bcl-2. The expression ratio of p53 by the expression ratio of bcl-2 and the expression ratio of Ki-67 by the expression ratio of p53 had a positive relationship in all of the Gleason score 6 and the Gleason score $9{\sim}10$ (P<0.05). However, the expression ratio of Ki-67 by the expression ratio of bcl-2 did not show any significance in the Gleason score $9{\sim}10$ (P<0.05). Therefore, the results suggested that p53 expression could be used as an independent prognostic factor.

• Nuclear Medicine and Molecular Imaging
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• 제41권3호
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• pp.260-262
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• 2007

A 68-year-old man with small cell carcinoma of the lung and adenocarcinoma of the prostate underwent Tc-99m MDP bone scintigraphy for the evaluation of skeletal metastases. Bilateral symmetrical photon defects in both parietal bones of the skull were observed. The radiographs of the skull demonstrates biparietal thinning in the same area of the abnormality identified on bone scintigraphy. Although these findings in cancer patients can be mistaken for skeletal metastases, the symmetry and location of the photon defects are generally indicative of biparietal thinning.

• Asian Pacific Journal of Cancer Prevention
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• 제13권5호
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• pp.1719-1722
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• 2012

The Wiskott-Aldrich Syndrome Protein family Verprolin - homologous proteins (WAVEs), encoded by a metastasis promoter gene, play considerable roles in adhesion of immune cells, cell proliferation, migration and destruction of foreign agents by reactive oxygen species. These diverse functions have lead to the hypothesis that WAVE proteins have multi-functional roles in regulating cancer invasiveness, metastasis, development of tumor vasculature and angiogenesis. Differentials in expression of WAVE proteins are associated with a number of neoplasms include colorectal cancer, hepatocellular cancer, lung squamous cell carcinoma, human breast adenocarcinoma and prostate cancer. In this review we attempt to unify our knowledge regarding WAVE proteins, focusing on their potentials as diagnostic markers and molecular targets for cancer therapy.

• BMB Reports
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• 제41권10호
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• pp.722-727
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• 2008

The present study compared the proteomic characteristics of a low passage number (L-33) and high passage number (H-81) LNCaP cell clone. Marked differences in protein expression were noted in the response of L-33 and H-81 cells to androgens. To investigate if regulation of these proteins was androgen-dependent, expression of the androgen receptor was silenced via small interfering RNA. Consistent with the proteomic data, abrogation of androgen receptor production in H-81 cells resulted in the reversed expression level into L-33 cells compared with non-treated H-81 LNCaP cells. The results clarify the progression into an androgen-independent phenotype.

• 통합자연과학논문집
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• 제11권1호
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• pp.25-29
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• 2018

Protein phosphatase manganese dependent 1D (PPM1D), a Ser/Thr protein phosphatise, play major role in the cancer tumorigenesis of various tumors including neuroblastoma, pancreatic adenocarcinoma, medulloblastoma, breast cancer, prostate cancer and ovarian cancer. Hence, analysis on the structural features required for the formation of PPM1D-inhibitor complex becomes essential. In this study, we have performed molecular docking of SL-175 and -176 and protein-protein docking of CDC5L with PPM1D. On analysing the docked complexes, we have identified the important residues involved in the formation of protein-ligand complex. Research concentrating on these residues could be helpful in understanding the pathophysiology of various tumors related to PPM1D.

• 통합자연과학논문집
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• 제9권1호
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• pp.35-40
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• 2016

Protein phosphatase manganese dependent 1D (PPM1D) is one of the Ser/Thr protein phosphatases belongs to the PP2C family. They play an important role in cancer tumorigenesis of various tumors including neuroblastoma, pancreatic adenocarcinoma, medulloblastoma, breast cancer, prostate cancer and ovarian cancer. Even though PPM1D is involved in the pathophysiology of various tumors, the three dimensional protein structure is still unknown. Hence in the present study, homology modelling of PPM1D was performed. 20 different models were modelled using single- and multiple-template based homology modelling and validated using different techniques. Best models were selected based on the validation. Three models were selected and found to have similar structures. The predicted models may be useful as a tool in studying the pathophysiological role of PPM1D.

• 대한화학회지
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• 제63권2호
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• pp.85-93
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• 2019

A panel of chalcone-sulphonamide hybrids has been designed by tethering appropriate sulphonamide scaffold with substituted chalcones as a multi-target drug for anticancer screening. Chalcones were prepared by Claisen-Schmidt condensation reaction of a substituted aldehyde with para aminoacetophenone. All the synthesized compounds were evaluated against selected five cancer cell lines, MCF-7 (Breast cancer), DU-145 (Human prostate Carcinoma), HCT-15 (Colon cancer), NCIH-522 (stage 2, adenocarcinoma; non-small cell lung cancer) and HT-3 (Human cervical cancer). Most of the synthesized chalcone-sulphonamide hybrids showed amended cytotoxic activity against various cancer cell lines which may be attributed to the linkage of sulphonamide with chalcone skeleton. The synthesized compounds were characterized by FT-IR, $^1H$ NMR, $^{13}C$ NMR and HR-LCMS and spectral study assert the structures of synthesized sulphonamide-chalcone hybrids.

• 생명과학회지
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• 제28권4호
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• pp.465-471
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• 2018

벌 화분은 오랫동안 전 세계적으로 이용되어 온 대체요법 중 하나이다. 벌 화분은 항진균 및 항균작용, 항암작용, 면역조절, 그리고 세포의 증식 등 다양한 생리활성을 갖고 있는 것으로 알려져 있다. 본 연구는 설치류를 이용하여 벌 화분의 양성전립선비대증의 개선 효과를 밝히기 위해 진행되었다. 벌 화분은 활성 성분의 추출률을 극대화하고, 생체 흡수율을 높이기 위하여 나노 크기로 분쇄하여 이용하였다. 먼저, 나노 크기의 벌 화분은 만성 testosterone 투여에 의한 전립선 크기 증가를 유의하게 완화하였다. 게다가 나노 크기의 벌 화분은 혈중 전립선 특이 항원의 농도를 뚜렷하게 감소시켰다. 흥미롭게도 나노 크기의 벌 화분은 testosterone에 의한 혈중 prostaglandin $E_2$ 증가에는 유의한 영향을 미치지 않았다. 이러한 나노 크기 벌 화분의 약리 효능은 dutasteride의 효과와 유사하였다. 마지막으로 나노 크기의 벌 화분은 androgen-반응성 인간 전립선 샘암종 세포인 LNCaP 세포의 손상을 유도하지 않았다. 이러한 결과를 종합하면, 나노화 된 벌 화분은 양성전립선비대증의 치료에 대체요법으로 이용될 수 있을 것으로 기대된다.

• 한국식품영양과학회지
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• 제36권1호
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• pp.1-7
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• 2007

된장제조 중 녹차추출물 첨가 및 죽염을 사용할 경우 돌연변이 억제작용 및 in vitro 항암 기능이 증진되는 것으로 나타났다. 돌연변이원 $AFB_1$에 대해 시료처리 농도 1.25 mg/plate에서는 녹차추출물을 첨가하지 않은 된장, 1% 및 2% 첨가 된장은 각각 63%, 85%, 87%의 항돌연변이 효과를 보여서 녹차 첨가에 의해 활성이 현저히 높아졌으나 녹차 1% 와 2%첨가에서 큰 차이는 보이지 않았다. MNNG에 대해서도 이러한 경향은 마찬가지였고, 녹차추출물 1% 첨가된장에 죽염을 사용했을 때는 더욱 높은 항돌연변이 효과를 나타냈다. 녹차추출물을 첨가한 된장의 in vitro 항암효과를 살펴 본 결과, PC-3인체 전립선암세포에서 0.5 mg/mL로 시료를 처리했을 때 대조군인 일반된장이 46%의 항암활성을 나타낸 데 비하여 녹차추출물을 1% 첨가한 시료는 70%, 2% 첨가한 시료는 77%의 암세포 생존저해효과를 나타내어 첨가 농도가 증가할수록 인체 전립선암세포의 생존을 더 크게 억제함을 알 수 있었다. 또한 죽염을 사용했을 때(녹차 1% 첨가군) 항암활성이 더욱 높아졌으며, 녹차 2% 첨가와 비슷하거나 더 높은 항암효과를 보였다. 다른 인체 전립선암세포인 DU145를 이용한 항암효과 실험에서도 같은 경향을 보여서 녹차추출물을 첨가하거나 죽염을 사용함으로써 된장의 항돌연변이 및 항암활성을 증진시킴을 알 수 있었다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권16호
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• pp.6963-6966
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• 2015

Background: During the past few years, Hesa-A, a herbal-marine mixture, has been used to treat cancer as an alternative medicine in Iran. Based on a series of studies, it is speculated that Hesa-A possesses special cytotoxic effects on invasive tumors. To test this hypothesis, we investigated the selective anticancer effects of Hesa-A on several cancer cell lines with different metastatic potential. Materials and Methods: Hesa-A was prepared in normal saline as a stock solution of 10 mg/ml and further diluted to final concentrations of $100{\mu}/ml$, $200{\mu}g/ml$, $300{\mu}g/ml$ and $400{\mu}g/ml$. MTT-based cytotoxicity assays were performed with A549 (lung non small cancer), MCF-7 (breast adenocarcinoma), SKOV3 (ovarian cancer), and PC-3 (prostate adenocarcinoma) cells. Results: All treated cancer cells showed significant (P<0.01) or very significant (P<0.0001) differences in comparison to negative control at almost all of the tested doses ($100-400{\mu}g/ml$). At the lower dose ($100{\mu}g/ml$), Hesa-A reduced cell viability to 66%, 45.3%, 35.5%, 33.2% in SKOV3, A549, PC-3 and MCF-7 cells, respectively. Moreover, at the highest dose ($400{\mu}g/ml$), Hesa-A resulted in 88.5%, 86.6%, 84.9% and 79.3% growth inhibition in A549, MCF-7, PC-3 and SKOV3 cells, respectively. Conclusions: Hesa-A exert potent cytotoxic effects on different human cancer cells, especially those with a high metastatic potential.