• 한국가축번식학회지
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• 제4권1호
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• pp.13-19
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• 1980

This experiment was carried out to observe early morphological changes of luteal cells in Korean native cattle treated with prostaglandin F2$\alpha$. Twenty five gram of prostaglandin F2$\alpha$ was administrated per cow at 10 days after ovulation and luteal cells were removed 30, 60, 120 and 180 minites after administration. Morphological changes of each luteal cell was observed by electron microscope. the results obtained were summarized as followings: 1. Many electron-dense granules were observed in luteal cells obtained from control cow but those granules were decreased rapidly after 30 minutes of administration and no granules were obresved after 180 minutes of administration. 2. In control, the shape of mitochondria were begining to collapse from the time of 60 minutes after administration. After 180 minutes of administration, mitochondria were swelled extreamly. 3. Lipid droplets in luteal cell were increased in its size and number with the duration of time after administration. 4. Shape of smooth endoplasmic recticulum was vesicular and its dimension and number were increased according to time course after administration.

• Reproductive and Developmental Biology
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• 제39권1호
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• pp.1-6
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• 2015

Luteolysis is a cyclical regression of the corpus luteum in many non-primate mammalian species. Prostaglandin $F_2{\alpha}$($PGF_2{\alpha}$) from the uterus and ovary induces functional and structural luteolysis in bovine. The action of $PGF_2{\alpha}$ is mediated by $PGF_2{\alpha}$ receptor located on the luteal steroidogenic and endothelial cell membranes. $PGF_2{\alpha}$ plays an important role in regulating nitric oxide production in endothelial cells of the bovine corpus luteum. Nitric oxide production and nitric oxide synthase activity are stimulated and induced by $PGF_2{\alpha}$ in luteal endothelial cells. Moreover, the reactive oxygen species inhibits progesterone secretion in bovine luteal cells and induces apoptosis. Thus, the interaction between $PGF_2{\alpha}$ and reactive oxygen species provides important aspects in physiology of the corpus luteum forfunctional and structural luteolysis.

• Journal of Nutrition and Health
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• 제27권6호
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• pp.574-582
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• 1994

The effects of age and dietary fatty acid composition on prostagladin production was investigated in Sprague-Dawley strain male rats. Animals weighing 88.6$\pm$2.2g were fed 10% dietary fat(W/W, 20% of total energy). The P/S ratios of dietary lipid were three levels(0.5, 1, 2) and there were three different levels of n-6/n-3 fatty acid ratio(2, 4, 8) in each P/S ratio. The experimental period were 1 month and 12 months, respectively. The results of this study were as follows. As the age of rats increased, the plasma thromboxane B2 production increased, but aorta 6-keto prostaglandin F1$\alpha$ decreased. When a higher amount of n-3 fatty acid was fed in each P/S ratio, the relative percentage of linolenic acid and EPA in platelet increased.

• The Korean Journal of Physiology
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• 제22권1호
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• pp.31-39
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• 1988

This study was carried out to investigate the action of adenosine triphosphate (ATP) on the motility of immature pig uterine smooth muscle. ATP appeared contractile responses in a dose-dependent manner, showing the maximal contraction at the concentration of $10^{-3}M$ in the uterine smooth muscle strip. The contractile responses by $ATP(10^{-4}M)$ were not affected by atropine $(10^{-6}M)$, phentolamine $(10^{-6}M)$, propranolol $(10^{-6}M)$, pyrilamine $(10^{-6}M)$, cimetidine $(10^{-6}M)$, and theophyulline $(5{\times}10^{-5}M)$, but were inhibited uncompetitively by quinidine. The effects of these drugs on the contractile responses by prostaglandin $F_{2{\alpha}}(PGF_{2{\alpha}})$ were also comparable to those observed with ATP. When muscle strips were pretreated with indomethacin $(5{\times}10^{-5}M)$ for 20 min., the contractile responses by $ATP(10^{-4}M)$ were completely inhibited. But the contractile responses by $PGF_{2{\alpha}}$ were not affected by indomethacin. These results suggest that ATP elicited the contraction through noncholinergic- and nonadrenergic-receptor mediated by prostaglandin $F_{2{\alpha}}$ in pig uterine smooth muscle.

• 한국가축번식학회지
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• 제3권2호
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• pp.50-56
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• 1979

This experiment was conducted to clarity the possibility of practical use of farrowing inductionin sow by the administration of prostaglandin F2$\alpha$. For this experiment, total 320 heads of pregnant sow and its piglets were used. The reproductive characteristics of artificially farrowed sow and, health and growth state of piglets were estimated. The results obtained in this experiment were summarized as following: 1. No significant difference were observed between naturally and artifically farrowed sow in several aspects such as the rate of dystocia, length of farrowing, farrowing intervals from piglet to piglet. 2. significant (P<0.05) differences were observed between naturally and artificially farrowed sow in intervals from weaning to estrus. However, there were no significant differences among those of 5, 7.5 and 10mg treated group. 3. There were no differences in number of stillbirth, immature birth and alive piglets at 3 weeks age per litter were observed. 4. Similar birth weight, weaning weight, daily gain and rearing rate of piglets were obtained from both naturally and artificially farrowing.

• Asian-Australasian Journal of Animal Sciences
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• 제13권3호
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• pp.390-400
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• 2000

The corpus luteum (CL) is a temporary endocrine gland whose main function is to secrete progesterone to support pregnancy. On the other hand, the cyclic bovine CL has also been shown to be a site of prostaglandin $F_{2{\alpha}}$ ($PGF_{2{\alpha}}$) production. Although there is general agreement that endometrial $PGF_{2{\alpha}}$ is an essential luteolysin in cattle, luteal $PGF_{2{\alpha}}$ seems to play a luteotropic role as an autocrine and/or paracrine factor, especially for the development and maintenance of the CL. This supposition is based on evidence that some of the prerequisites for autocrine/paracrine mechanisms are present, including local production of $PGF_{2{\alpha}}$ and the existence of specific binding sites within the CL. The purpose of this paper is to review the regulation of luteal $PGF_{2{\alpha}}$ secretion, its action on CL as an autocrine and/or paracrine factor and the receptivity of bovine CL to. $PGF_{2{\alpha}}$.

• The Korean Journal of Physiology and Pharmacology
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• 제4권4호
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• pp.301-309
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• 2000

The purpose of our investigation was to examine the effects of prostaglandin $F_{2{\alpha}}\;(PGF_{2{\alpha}})$ on membrane potentials, $Ca^{2+}-activated\;K^+\;(K_{Ca})$ channels, and delayed rectifier $K^+(K_V)$ channels using the patch-clamp technique in single rabbit middle cerebral arterial smooth muscle cells. $PGF_{2{\alpha}}$ significantly hyperpolarized membrane potentials and increased outward whole-cell K currents. $PGF_{2{\alpha}}$ increased open-state probability of $K_{Ca}$ channels without the change of the open and closed kinetics. $PGF_{2{\alpha}}$ increased the amplitudes of $K_V$ currents with a leftward shift of activation and inactivation curves and a decrease of activation time constant. Our results suggest that the activation of $K_{Ca}$ and $K_V$ channels, at least in part, may lead to attenuate or counteract vasoconstriction by $PGF_{2{\alpha}}$ in middle cerebral artery.

• 한국임상수의학회지
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• 제3권2호
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• pp.317-324
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• 1986

A total of 600 Holstein cows in Chonnam province were examined to make a diagnosis on the ovarian diseases. By clinical signs and rectal examinations, 120 cows were founded to have ovarian diseases such as 40 cows with suboestrus, 40 cows with persistent corpus luteum, 40 cows with luteal cysts. The Prostaglandin $F_{2{\alpha}}$ (PG $F_{2{\alpha}}$) was administered intramuscularly and intradermic vaginally ; also intraovarian injections and intrauterine infusions was made. Comparisons were made on the effect of PG $F_{2{\alpha}}$ on the ovarian diseases. The results obtained were summarized as follows. 1. Induction of normal estrum after PG $F_{2{\alpha}}$ treatment was 75.0～82.5%(mean 78.3%). The conception rate was 83.3～84.8% (mean 84.0%) and average services per conception was 1.6～l.8(mean 1.7). Days from treatment to estrum was 3.9～4.3(mean 4.1) days. 2. Average conception rate was different according to the routes of administration: intraovary 91.7%, intra-uterus 88.0%, intramuscle 80.9% and intradermic vagina 75.0%, respectively.

• 한국가축번식학회지
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• 제26권2호
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• pp.183-192
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• 2002

본 연구는 임신 및 분만후 rat에 oxytocin을 투여후 혈장, 자궁 및 태반 조직에서 estradiol-17$\beta$, progesterone, prostaglandin F$_2$$_{\alpha}$ 및 prostaglandin E$_2$그리고 자궁 조직의 oxytocin 수용체의 함량 사이의 관계에 대하여 조사하였다. 임신 rat에 oxytocin 투여 후 혈장 estradiol-17$\beta$의 농도는 임신 말기에 서서히 증가하여 임신 22일에 최고를 기록하였고 분만 후 1일에는 임신 16일 수준으로 감소하였다. Progesterone 농도는 임신 18일부터 분만 후 1일에 유의성 있는(p<0.05) 감소를 보였다. Prostaglandin F$_2$$_{\alpha}$ 및 Prostaglandin E$_2$농도는 임신 14일에서 임신 22일에 공히 상승하여 임신 22일에 최고를 기록하였고 분만 후 1일에는 급격히 감소하였다. Estradiol-17$\beta$의 농도는 자궁 조직에서 임신 14일에서 20일까지 서서히 증가하다가 임신 22일에 급격한 증가를 기록하였고 분만 후 1일에는 급격히 감소하여 임신 14일 수준을 유지하였으며 progesterone 농도는 임신 16일에 상승을 보인 후 임신 20일까지 급격히 감소하였고 분만 후 1일까지 같은 수준을 유지하였다. Prostaglandin F$_2$$_{\alpha}$ 및 prostaglandin E$_2$농도는 자궁조직에서 임신 말기 전기간에 상승하여 임신 22일에 최고를 기록한 후 분만 후 1일에는 급격히 감소하여 임신 14일과 비교하여 70% 수준을 기록하였다. Prostaglandin F$_2$$_{\alpha}$ 농도는 태반 조직에서 임신 14일에서 임신 22일까지 지속적으로 증가하였고 분만 후 1일에는 급격히 감소하였으며 prostaglandin E$_2$의 농도는 임신 14일에서 20일에 증가한 후 분만 후 1일까지 감소하였다. Oxytocin 수용체의 농도는 자궁 조직에서 임신 20일에서 임신 22일 사이에 급격히 증가한 후 분만 후 1일까지 비슷한 수준을 기록하였다. 이상에서와 oxytocin투여에 의한 임신 rat에 있어서 progesterone 농도의 감소에 이은 estradiol-17 $\beta$, prostaglandin F$_2$$_{\alpha}$, prostaglandin E$_2$및 oxytocin 수용체 농도의 증가는 조기에 시작되었다.

• 대한약리학회지
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• 제14권1_2호
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• pp.25-31
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• 1978

It has recently been reported that prostaglandin $(PG)F_{2\alpha}$ produces diruesis and natriuresis when given directly into a lateral ventricle of the brain in rabbits (Kook et al). In this study attempts were made to elucidate the mechanism of the natriuresis utilizing dogs. In mongrel dogs of both sexes weighing 9-l2kg, a lateral ventricle of the cerebrum was cannulated and the agent was delivered in less than 0.2ml of 0.9% saline. $PGF_{2\alpha}$, $100\;{\mu}g$, ivt, elicited mild diuresis, while glomerular filtration rate and systemic blood pressure remained unchanged. In 8 chloralso-anesthetized, hydrated dogs undergoing water diuresis, ivt $PGF_{2\alpha}$ produced antidiuresis concomitant with marked natriuresis, which resembles the action of large doses of Arginine vasopressin in doses of 0.3-1.5mu/kg/min produced marked natriuresis. In 9 experiments, $PGF_{2\alpha}$ was given intraventricularly during the maximal diuresis induced by ADH. The kidney responded with significant natriuresis though less marked and transient than during water diuresis. It is thus concluded that beside ADH still other natriuretic factor(s) may be involved in the natriuresis induced by ivt $PGF_{2\alpha}$ in the dog.