• Title/Summary/Keyword: Propagation methods

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In vitro Micropropagation and Root Induction of Pear Genetic Resources

  • Jae-young Song;Jinjoo Bae;Woohyung Lee;Jung-ro Lee;Munsup Yoon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2022.09a
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    • pp.63-63
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    • 2022
  • Pear (Pyrus spp.) is a typical fruit and grown in the temperate climate regions throughout the world. Development of appropriate methods for in vitro propagation and root induction are important to increase the production rate and plant quality rapidly. This study was conducted to find the most appropriate media conditions for in vitro propagation and rooting of three pear cultivars, 'Barttlett', 'BaeYun No.3' and 'Oharabeni'. In vitro propagation was induced on Murashige and Skoog medium (MS) with 2.0 mg/L N6-benzyladenine (BA) and 0.2 mg/L indole-3-butyric acid (IBA) medium. For root induction of these cultivars, the shoot explants of the propagated plants were cultured on two different media containing 1/2 MS medium containing 0.2 mg/L IBA with 15 g/L Sucrose (Rooting Medium 1, RM1) and 1/4 Linsmaier and Skoog medium (LS) medium containing 1 mg/L IBA and 1 mg/L NAA hormone with 7.5 g/L glucose (Rooting Medium, RM2) and after 2 weeks, the plants on the RM2 medium are transferred on RM1 medium (RM2 condition). After nearly seven weeks, percentage of rooting formation were 22.2% in RM1 and 30% in RM2 conditions for Barttlett and 70% in RM1 and 60% in RM2 conditions for Oharabeni cultivars. No differences in these cultivars were observed between RM1 and RM2 conditions. However, BaeYun No.3 cultivar was observed 0% in RM1 and 72.7% in RM2 conditions. This study will help to propagation and root induction of in vitro plants for various pear cultivars.

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Study on Application of Ultrasonic Propagation Imager for Non-destructive Evaluation of Composite Lattice Structure (복합재 격자 구조 비파괴평가를 위한 초음파전파 영상화 시스템 활용 연구)

  • Park, Jae-Yoon;Shin, Hye-Jin;Lee, Jung-Ryul
    • Composites Research
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    • v.30 no.6
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    • pp.356-364
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    • 2017
  • Composite lattice structures are tried to be used in various fields because of its benefit in physical properties. With increase of demand of the composite lattice structure, nondestructive testing technology is also required to certificate the quality of the manufactured structures. Recently, research on the development of the composite lattice structure in Republic of Korea was started and accordingly, fast and accurate non-destructive evaluation technology was needed to finalize the manufacturing process. This paper studied non-destructive testing methods for composite lattice structure using laser ultrasonic propagation imaging systems. Pulse-echo ultrasonic propagation imaging system was able to inspect a rib structure wrapped with a skin structure. To reduce the time of inspection, a band divider, which can get signal in different frequency bands at once, was developed. Its performance was proved in an aluminum sandwich panel. In addition, to increase a quality of results, curvature compensating algorithm was developed. On the other hand, guided wave ultrasonic propagation imaging system was applied to inspect delamination in a rib structure. To increase an area of inspection, multi-source ultrasonic wave propagation image was applied, and defects were successfully highlighted with variable time window amplitude mapping algorithm. These imply that ultrasonic propagation imaging systems provides fast and accurate non-destructive testing results for composite lattice structure in a stage of the manufacturing process.

Shape Design Optimization of Crack Propagation Problems Using Meshfree Methods (무요소법을 이용한 균열진전 문제의 형상 최적설계)

  • Kim, Jae-Hyun;Ha, Seung-Hyun;Cho, Seonho
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.27 no.5
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    • pp.337-343
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    • 2014
  • This paper presents a continuum-based shape design sensitivity analysis(DSA) method for crack propagation problems using a reproducing kernel method(RKM), which facilitates the remeshing problem required for finite element analysis(FEA) and provides the higher order shape functions by increasing the continuity of the kernel functions. A linear elasticity is considered to obtain the required stress field around the crack tip for the evaluation of J-integral. The sensitivity of displacement field and stress intensity factor(SIF) with respect to shape design variables are derived using a material derivative approach. For efficient computation of design sensitivity, an adjoint variable method is employed tather than the direct differentiation method. Through numerical examples, The mesh-free and the DSA methods show excellent agreement with finite difference results. The DSA results are further extended to a shape optimization of crack propagation problems to control the propagation path.

Propagation Characteristics of Rare Plants Diabelia spathulata (Siebold & Zucc.) Landrein Applied by Seeding and Cutting Methods (종자파종과 삽목을 활용한 희귀식물 주걱댕강나무의 증식 특성)

  • Jeong Gul Jang;Myung Hoon Yi
    • Korean Journal of Plant Resources
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    • v.36 no.4
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    • pp.390-401
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    • 2023
  • The purpose of this study was to identify the propagation characteristics of Diabelia spathulata (Siebold & Zucc.) Landrein through seeding and cutting methods. The seed germination rate was meager (0.22~3.56%). In hardwood cutting, the highest rooting rate was 36.1% in sand + perlite (2:1) and 37.8% in 1000 PPM of IBA pre-treatment. Softwood cutting showed the highest rooting rate when the cutting diameter was 3 to 5 mm and the cutting length was 7 to 8 cm. In particular, the highest rooting rate of 80% was observed in the sand among the 8 cultivation media treatments. In the case of root cuttings, the rooting rate was 83.3~96.7% in all cultivation media. The highest rooting rate was observed when the diameter of the cutting was 7 to 8 mm, and the length of the cutting was about 10 to 11 cm. Among cutting methods, root cutting showed the highest rooting rate. Division showed a 100% rooting rate regardless of size or growth status. For Diabelia spathulata (Siebold & Zucc.) Landrein, propagation by cuttings was more efficient than seeding. However, Further research on the morphophysiological dormancy and dormancy break of seeds is needed for seed propagation.

The Prediction and Analysis of the Propagation Characteristics in Indoor Environments Using the SBR/Image Method (SBR 및 영상기법을 이용한 실내 환경의 전파특성 예측과 분석)

  • 손호경;김채영;김성진
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.12 no.2
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    • pp.199-207
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    • 2001
  • In this paper, the technique of prediction and analysis on the characteristics of propagation in indoor environment is presented. This technique needs no reception sphere commonly used in 3D-ray tracing scheme, and thereby it lends us easy code realization. The validity of developed code is verified by comparing with the values of image methods and measurement. The developed technique applied to the structure of rectangular corridor with the iron door and we calculated the path loss for the variation of the iron door angle. The path loss decreased about 15 dB at the distance of30 m from the iron door and the delay spread increased approximately by four times. Based on the computation, we confirmed that indoor propagation in PCS is heavily affected by the iron door in corridor.

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Analysis of Arrayed Waveguide Grating Waveglength Filter using Wide Angle Beam Propagation Method (Wide Angle BPM 을 이용한 광도파로열 격자 파장 필터의 해석)

  • Park, Jun-O;Jeong, Yeong-Cheol
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.39 no.2
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    • pp.46-55
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    • 2002
  • The key component to accomplish the WDM all optical network is an Arrayed Waveguide Grating(AWG) wavelength filter Numerical analysis is necessary for design and analysis of optical components like AWG wavelength filter. Beam Propagation Method(BPM) is the most widely-used method. In this paper, we analyze the difference between the paraxial BPM and the WA-BPM when they are applied to the analysis of InP/InGaAsP/InP AWG wavelength filter. The paraxial BPM is based on paraxial approximation, and the WA-BPM is based on the low order Pade approximant. The side lobe level(SLL) and insertion loss calculated from both methods are compared. The high order Pade approximant will to used to more accurate design and analysis of AWG.

Research on the Propagation Mode Theory of Marine RFID in the Fresnel Zone (프레넬 영역에서의 해상용 RFID 전파모드 이론 고찰)

  • Yim Jeong-Bin;Ku Ja-Young;Lee Jae-Eung
    • Proceedings of KOSOMES biannual meeting
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    • 2005.11a
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    • pp.65-69
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    • 2005
  • The theoretical propagation modes of radio waves in the area of Line of Sight(LOS) within Fresnel zone are searched for the available detection ranges in a Marine RFID (Radio Frequency Identification). The structural LOS model to Earth's curvature is proposed and, the calculation method of horizontal distance in a specific radio frequency is also considered in this work As studying results, it is found that the height of antenna to cover the detection ranges and the influences of detection ranges by weather environments can be analyse with the theoretical methods.

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A Conflict Detection Method Based on Constraint Satisfaction in Collaborative Design

  • Yang, Kangkang;Wu, Shijing;Zhao, Wenqiang;Zhou, Lu
    • Journal of Computing Science and Engineering
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    • v.9 no.2
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    • pp.98-107
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    • 2015
  • Hierarchical constraints and constraint satisfaction were analyzed in order to solve the problem of conflict detection in collaborative design. The constraints were divided into two sets: one set consisted of known constraints and the other of unknown constraints. The constraints of the two sets were detected with corresponding methods. The set of the known constraints was detected using an interval propagation algorithm, a back propagation (BP) neural network was proposed to detect the set with the unknown constraints. An immune algorithm (IA) was utilized to optimize the weights and the thresholds of the BP neural network, and the steps were designed for the optimization process. The results of the simulation indicated that the BP neural network that was optimized by IA has a better performance in terms of convergent speed and global searching ability than a genetic algorithm. The constraints were described using the eXtensible Markup Language (XML) for computers to be able to automatically recognize and establish the constraint network. The implementation of the conflict detection system was designed based on constraint satisfaction. A wind planetary gear train is taken as an example of collaborative design with a conflict detection system.

Analysis of Operational State and Radio Environment of AIS (AIS의 운용현황과 전파환경에 대한 분석 연구)

  • Seo Ki-yeol;Hong Tae-ho;Park Gyei-kark;Choi Jo-Cheon
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.9 no.7
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    • pp.1439-1444
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    • 2005
  • AIS needs hish efficiency and safety in terms of operational management and, in the aspect of service range, it is time to minimize shadow regions through propagation evaluation of AIS transmitter. Thus this study analyzes shadow regions and the scope of propagation of waves from stations through the electric wave environment of AIS. First, this study examines the characteristics of AIS wave and draws up methods to analyze the scope of propagation of waves according to the characteristics. Second, this study finds out service regions and shadow regions of the 22 stations across the nation by analyzing the actual data based on climate and topography. Lastly, this study summarizes the results of wave conductivity evaluation and conductivity problems and proposes improvement measures.

Propagation of lymphocystis disease virus (LCDV) in the FIN cell line originated from olive flounder Paralichthys olivaceus fin

  • Hossain, Mosharrof;Kim, Wi-Sik;Jung, Sung-Ju;Oh, Myung-Joo
    • Journal of fish pathology
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    • v.24 no.2
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    • pp.47-51
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    • 2011
  • The present study demonstrated lymphocystis disease virus (LCDV) propagation through cytopathic effects (CPE) formation and LCDV detection in olive flounder fin (FFN) cells by polymerase chain reaction (PCR) and fluorescent antibody technique (FAT) methods. Tissue filtrates from the cluster cells produced CPE in FFN cells, which initially cells became enlarged and gradually underwent fusion en masse. Infectivity of culture grown LCDV using the FFN cells reached $10^{2.3}$ $TCID_{50}$/ml at 4 days post infection and the highest titer was measured $10^{6.5}$ $TCID_{50}$/ml at 12 days. The viral DNA was detected in the cell culture supernatants showing CPE and the CPE cells by PCR. Antigen specific strong fluorescence reacting with monoclonal antibody against the virus revealed the presence of viral antigen in the cytoplasm of infected FFN cells. These results suggest that the FFN cell line originated from the olive flounder has a susceptibility of the LCDV.