• Korean Journal of Animal Reproduction
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• v.22 no.4
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• pp.341-347
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• 1998

This study was conducted to determine changes in circulating progesterone and Insulin-like Growth Factor-1(IGF-1) concentrations according to daily gain of 0.5 and 0.7 kg ; from 33 weeks of age until the onset of regular oestrus cycles in the heifer of Hanwoo. In all animals, progesterone concentrations was undetectable until just before first oestrus. A small progesterone elevation of approximately 6 days duration(based upon the mean progesterone profile) preceded the first oestrus in 13 heifers. The mean age at first oestrus of the daily gain 0.7 kg(331.0$\pm$ 15.0 days) group was earlier than those of the daily gain 0.5 kg(358.9$\pm$7.9 days) group, and the mean weight at first oestrus of the daily gain 0.7 kg(236.0$\pm$4.7 kg) group was heavier than those of the daily gain 0.5 kg(224.8$\pm$9.7 kg) group. IGF-1 concentrations from day 3 to 15 of first oestrus were higher than those of the earlier luteal stage(Day 0~1) and the luteal regression stage (Day 18~20). IGF-1 concentrations according to the growth stage (from 33 weeks to 57 weeks) increased gradually. On the basis of these results, we suggest that the onset of first oestrus in Hanwoo heifer is about 345 day of age, and progesterone concentrations are closely related to the IGF-1 concentration.

• Clinical and Experimental Reproductive Medicine
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• v.17 no.1
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• pp.87-91
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• 1990

Development of a solid-phase chemiluminescence immunoassay for the detection of progesterone in serum extract was described. The chemiluminescence immunoassay was establised utilizing anti-progesterone monoclonal antibody that coated on polystyrene tubes and progesterone-ABEI conjugate as tracer. The light yield generated from antibody bound conjugate was counted on clinilumat luminometer by oxidation with microperoxidase and peroxide. The chemiluminescence immunoassay was high specific and accurate and detects as little as 3.9ng/ml of progesterone. The intra-assay CV ranged from 6% to 11.5% and inter-assay CV ranged from 13.6% to 18.7%. This assay system was good correlated with conventional kit radioimmunoassay system (r=0.98).

• The Korean Journal of Physiology and Pharmacology
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• v.17 no.5
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• pp.423-426
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• 2013

Females are more often affected by constipation than males, especially during pregnancy, which is related to the menstrual cycle. Although still controversial, alterations of progesterone and estrogen may be responsible. Therefore, this study was conducted in order to determine whether the female sex steroid hormone itself is responsible for development of constipation in both female and male mice. Administration of estrogen resulted in a decrease in weight of accumulated feces on days 2, 3, 4, and 5 in male mice and on day 5 in female mice, compared with the control group, but progesterone administration did not. Administration of estrogen resulted in a decrease in gastrointestinal movement, compared to normal; however, no significant change was observed by administration of progesterone. In conclusion, estrogen, rather than progesterone, may be a detrimental factor of constipation via decreased bowel movement in mice.

• Journal of Veterinary Clinics
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• v.21 no.3
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• pp.298-301
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• 2004

Pregnancy diagnosis by ultrasonography was performed for both pregnant and non-pregnant Asiatic black bears which were being under hibernation. Pregnancy was diagnosed for a pregnant bear by detecting images of heart-beat and vertebrae on ultrasonograph. Serum progesterone levels were measured for both pregnant and non-pregnant bears. The level of serum progesterone was 5.79 ng/ml for a pregnant bear and 0.76 ng/ml for a non-pregnant bear, respectively, thereby it was considered that measurement of serum progesterone level can be also useful for pregnancy diagnosis for Asiatic black bear.

• Korean Journal of Animal Reproduction
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• v.14 no.3
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• pp.165-173
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• 1990

A simple and sensitive microplate enzyme immunoassay(ELISA) was developed for progesterone, based on progesterone monoclonal antibody as anti-progesterone, horseradish peroxise(HRP) as enzyme-label and tetramethylbenzidine(TMB) as substrate. The assay has a sensitivity of 5pg-120pg/well and intra- and inter assay coefficients of variation for progesterone standard curve(0.1ng-3.2ng/ml) were ranged 4.4-10.6% and 5.-12.6%, respectively. They assay is performed in less than two hours and provide reliable values to differentiate among samples from day 0(A.I.), day 14 and day 19. The discriminatory levels for early pregnancy diagnosis are [>10ng(day 19) & decreasing rate <1.5 : pregnancy] and [ 7ng & decreasing rate 1.5 : non-pregnancy]. The accuracy of the pregnancy diagnosis for cows classified as positive(pregnancy) and negative(non-pregnancy) were 96% and 100%, respectively.

• Korean Journal of Veterinary Research
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• v.26 no.2
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• pp.225-228
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• 1986

Progesterone immunogen has been synthesized and its melting point, Rf-value, UV and IR spectrum have been measured to develope the essential step in antisera production against low molecular weight substance. Mixed anhydride reaction was used to conjugate $11{\alpha}$-hydroxy-progesterone with succinic anhydride. Melting point of one intermediate compound was $156^{\circ}C$, and Rf-value was 0.41 in benzene : acetone : methanol (5 : 5 : 2). Maximum absorbance was measured at 242nm and ${\varepsilon}$ was $1.641{\times}10^4cm^2/mole$. Loss of hydroxy group was observed at 3450nm, and carbonyl group was appeared at 1160nm, 1250nm and 2960nm. These results indicated that the intermediate compound was progesterone hemisuccinate. Maximum absorbance of progesterone bovine-serum albumin(BSA) conjugate was observed at 250nm. Molar ratio of progesterone to BSA was average 15.4 on UV spectrum.

• Journal of Veterinary Clinics
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• v.37 no.6
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• pp.305-310
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• 2020

To confirm the accuracy of the prediction of parturition day, the actual parturition days were compared with each day of the prediction of parturition day (n = 80). The accuracy of the prediction of parturition day was 80.0% (64/80) with a precision of ± 2 days from the first day of estrus after the first vaginal discharge, 97.5% (78/80) from the day when plasma progesterone concentrations increase above 4.0 ng/ml, and 72.5% (58/80) from the first day of diestrus, respectively. The accuracy of the prediction of parturition day by plasma progesterone concentration was higher than that by the first day of estrus and diestrus after the first vaginal discharge. These results indicated that the determination of plasma progesterone concentrations at estrus were a useful method for estimating of parturition day and for the reproductive management in pregnant bitches.

• Korean Journal of Veterinary Research
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• v.30 no.1
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• pp.35-40
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• 1990

This study was carried out to evaluate khe accuracy of the early diagnosis of pregnancy in dairy cows using EIK-kit of progesterone in milk and to compare the progesterone concentrations in milk with those assayed by radioimmunoassay. 1. The progesterone concentrations of the pregnant cows($2.07{\pm}0.54ng/ml$) were significantly higher than those of non-pregnant cows($1.04{\pm}0.19ng/ml$), and thereafter began to inerease and maintained high levels. 2. During 20 to 22 days after artificial insemination, the accuracy of pregnancy diagnosis from EIA-kit of progesterone were 93.7% for non-pregnant cows, and 91.6% for pregnant cows. 3. During 20 to 22 days after artificial insemination, the accuracy of pregnancy diagnosis from milk progesterone concentrations were 100% for non-pregnant cows (<2.7ng/ml), and 92.9% for pregnant cows($${\geq_-}3.2ng/ml$$). The average overall accuracy of prediction for pregnant and non-pregnant cows were 96.5%. 4. Accordingly, the pregnancy diagnosis from EIA-kit of progesterone is thought to be recommendable because this early diagnostic means are simple with accurate result.

• The Korean Journal of Physiology and Pharmacology
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• v.7 no.4
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• pp.217-221
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• 2003

To clarify the roles of gonadal steroids on pancreatic exocrine secretion, effects of progesterone and estradiol-$17{\beta}$ on spontaneous and secretagogue-induced exocrine response of isolated perfused rat pancreas were investigated. Intra-arterial infusion of progesterone resulted in significant increase of the spontaneous pancreatic fluid and amylase secretion dose-dependently. However, estradiol-$17{\beta}$ did not exert any influence on spontaneous pancreatic exocrine secretion. Exogenous secretin, cholecystokinin (CCK), and acetylcholine markedly stimulated pancreatic fluid and amylase secretion. Progesterone initially enhanced secretin-induced amylase secretion, but this stimulatory response declined thereafter to basal value. Moreover, secretin-induced fluid secretion was not affected by infusion of progesterone. Therefore, initial increase of secretion-induced amylase secretion by progesterone seems to be a non-specific action by washout effect of secretin. Estradiol-$17{\beta}$ failed to change the secretin-induced fluid and amylase secretion. Both progesterone and estradiol-$17{\beta}$ did not exert any influence on CCK-induced fluid and amylase secretion. Acetylcholine-induced exocrine secretion of isolated perfused pancreas also was not affected by intra-arterial infusion of progesterone or estradiol-$17{\beta}$. It is concluded from the above results that progesterone could enhance the spontaneous pancreatic fluid and amylase secretion of isolated perfused rat pancreas through non-genomic shortterm action, and that these effects could be masked by more potent stimulants such as secretin, CCK, and acetylcholine.

• Korean Journal of Animal Reproduction
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• v.27 no.3
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• pp.269-274
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• 2003

The purpose of this study was to determine the effect of rbST treatment on progesterone concentration, volume of luteal tissue and pregnancy rate following embryo transfer. Recipient cows were assigned to control and rbST group, of which was given a single injection of rbST (500 mg. sc) at estrus detection. The concentration of progesterone was not significantly different between control and at 0, 3, 6 days after rbST treatment. However, the concentration of progesterone at 9, 12 days was significantly higher than in control group (4.6 and 6.8 vs. 3.9 and 4.5 ng/ml P4). The pregnancy rate after embryo transfer in rbST treatment was significantly higher than in control group (64.0 vs. 47.1 %; p<0.05). The results indicated that rbST treatment in recipient cows could be improved the efficiency of pregnancy rate after embryo transfer.