• Title/Summary/Keyword: Progesterone

Search Result 898, Processing Time 1.067 seconds

한우 초발정 전후 혈중 Progesterone 및 Insulin-like Growth Factor-1 농도의 변화 (Change of Serum Progesterone and Insulin-like Growth Factor-1 Concentration during the First Oestrus in the Heifers of Hanwoo)

  • 고응규;성환후;백광수;나승환;최창용;신원집
    • 한국가축번식학회지
    • /
    • 제22권4호
    • /
    • pp.341-347
    • /
    • 1998
  • 본 연구는 생후 33 주령부터 초발정이 시작될 때까지 한우육성빈우의 일당증체량 (0.5kg, 0.7kg) 에 따른 혈중 progesterone 과 IGF-1 농도를 분석하여 한우의 초발정시기 및 춘기발동기전후 혈중 progesterone 과 IGF-1 농도를 비교 검토하기 위하여 수행하였다. 모든 시험축에서, 초발정 직전까지는 혈중 progesterone 농도는 검출할 수 없었으며, 13 두의 육성빈우에서 첫발정 6일전에 미미한 혈중 progesterone 농도가 상승하기 시작하여 짧은 황체기를 보였다. 초발정시기의 일당증체량 0.7kg 구의 평균 나이는 331.0$\pm$15.0 일로 0.5kg 구의 358.9$\pm$7.9 일보다 빨랐고 이때 체중도 0.7kg 구가 236.0$\pm$4.7kg으로 0.5kg 구의 224.8$\pm$9.7 보다 무거웠다. 초발정이후 3~15 일까지 IGF-1 농도는 황체초기 (0~1) 와 황체퇴행기 (18일~20일) 보다 높은 경향을 보였고, 성장단계에 따라서 생후 37주령부터 57주령까지 조사한 IGF-1 농도 변화는 37주령에 93.2$\pm$63.4ng/$m\ell$ 에서 57 주령에 170.9$\pm$59.35ng/$m\ell$로 점차적으로 상승하였다. 이상의 결과 한우의 초발정 평균나이는 345일령 전후로 추정되며 이시기에 혈중 progesterone 농도는 IGF-l 농도와 밀접한 관련이 있는 것 같다.

  • PDF

혈청내의 Progesterone 측정을 위한 Chemiluminescence Immunoassay의 개발에 관한 연구 (Development of Chemiluminescence Immunoassay for Progesterone in Serum)

  • 이기순;서병희;이재현;김종배
    • Clinical and Experimental Reproductive Medicine
    • /
    • 제17권1호
    • /
    • pp.87-91
    • /
    • 1990
  • Development of a solid-phase chemiluminescence immunoassay for the detection of progesterone in serum extract was described. The chemiluminescence immunoassay was establised utilizing anti-progesterone monoclonal antibody that coated on polystyrene tubes and progesterone-ABEI conjugate as tracer. The light yield generated from antibody bound conjugate was counted on clinilumat luminometer by oxidation with microperoxidase and peroxide. The chemiluminescence immunoassay was high specific and accurate and detects as little as 3.9ng/ml of progesterone. The intra-assay CV ranged from 6% to 11.5% and inter-assay CV ranged from 13.6% to 18.7%. This assay system was good correlated with conventional kit radioimmunoassay system (r=0.98).

  • PDF

Estrogen Rather Than Progesterone Cause Constipation in Both Female and Male Mice

  • Oh, Ji-Eun;Kim, Yong-Woon;Park, So-Young;Kim, Jong-Yeon
    • The Korean Journal of Physiology and Pharmacology
    • /
    • 제17권5호
    • /
    • pp.423-426
    • /
    • 2013
  • Females are more often affected by constipation than males, especially during pregnancy, which is related to the menstrual cycle. Although still controversial, alterations of progesterone and estrogen may be responsible. Therefore, this study was conducted in order to determine whether the female sex steroid hormone itself is responsible for development of constipation in both female and male mice. Administration of estrogen resulted in a decrease in weight of accumulated feces on days 2, 3, 4, and 5 in male mice and on day 5 in female mice, compared with the control group, but progesterone administration did not. Administration of estrogen resulted in a decrease in gastrointestinal movement, compared to normal; however, no significant change was observed by administration of progesterone. In conclusion, estrogen, rather than progesterone, may be a detrimental factor of constipation via decreased bowel movement in mice.

동면중 반달가슴곰에 대한 혈중 Progesterone치와 초음파진단기를 이용한 임신진단 (Pregnancy Diagnosis by Measuring Serum Progesterone Level and Ultrasonography for Asiatic Black Bear(Ursus thibetanus) Being under Hibernation)

  • 신남식;김용준;윤재원;김영준
    • 한국임상수의학회지
    • /
    • 제21권3호
    • /
    • pp.298-301
    • /
    • 2004
  • Pregnancy diagnosis by ultrasonography was performed for both pregnant and non-pregnant Asiatic black bears which were being under hibernation. Pregnancy was diagnosed for a pregnant bear by detecting images of heart-beat and vertebrae on ultrasonograph. Serum progesterone levels were measured for both pregnant and non-pregnant bears. The level of serum progesterone was 5.79 ng/ml for a pregnant bear and 0.76 ng/ml for a non-pregnant bear, respectively, thereby it was considered that measurement of serum progesterone level can be also useful for pregnancy diagnosis for Asiatic black bear.

단일클론항체를 이용한 Milk Progesterone Test(EIA) 측정법의 개발과 이에 의한 소의 발정 및 임신조기진단의 정확도 향상에 관한 연구 (Development of Milk Progesterone Test(EIA) using Monoclonal Antibody and It's Application to Estrus-and Early Pregnancy Detection in Dairy Cattle)

  • 김정우;홍승욱
    • 한국가축번식학회지
    • /
    • 제14권3호
    • /
    • pp.165-173
    • /
    • 1990
  • A simple and sensitive microplate enzyme immunoassay(ELISA) was developed for progesterone, based on progesterone monoclonal antibody as anti-progesterone, horseradish peroxise(HRP) as enzyme-label and tetramethylbenzidine(TMB) as substrate. The assay has a sensitivity of 5pg-120pg/well and intra- and inter assay coefficients of variation for progesterone standard curve(0.1ng-3.2ng/ml) were ranged 4.4-10.6% and 5.-12.6%, respectively. They assay is performed in less than two hours and provide reliable values to differentiate among samples from day 0(A.I.), day 14 and day 19. The discriminatory levels for early pregnancy diagnosis are [>10ng(day 19) & decreasing rate <1.5 : pregnancy] and [ 7ng & decreasing rate 1.5 : non-pregnancy]. The accuracy of the pregnancy diagnosis for cows classified as positive(pregnancy) and negative(non-pregnancy) were 96% and 100%, respectively.

  • PDF

프로제스테론의 면역원 합성에 관한 물리화학적 연구 (Physicochemical Study on the Synthesis of Progesterone Immunogen)

  • 박전홍;권종국
    • 대한수의학회지
    • /
    • 제26권2호
    • /
    • pp.225-228
    • /
    • 1986
  • Progesterone immunogen has been synthesized and its melting point, Rf-value, UV and IR spectrum have been measured to develope the essential step in antisera production against low molecular weight substance. Mixed anhydride reaction was used to conjugate $11{\alpha}$-hydroxy-progesterone with succinic anhydride. Melting point of one intermediate compound was $156^{\circ}C$, and Rf-value was 0.41 in benzene : acetone : methanol (5 : 5 : 2). Maximum absorbance was measured at 242nm and ${\varepsilon}$ was $1.641{\times}10^4cm^2/mole$. Loss of hydroxy group was observed at 3450nm, and carbonyl group was appeared at 1160nm, 1250nm and 2960nm. These results indicated that the intermediate compound was progesterone hemisuccinate. Maximum absorbance of progesterone bovine-serum albumin(BSA) conjugate was observed at 250nm. Molar ratio of progesterone to BSA was average 15.4 on UV spectrum.

  • PDF

반려견에서 혈중 Progesterone 농도 측정에 의한 분만일 예측 2. 분만예정일의 정확성 확인 (Prediction of Parturition Day by Determination of Plasma Progesterone Concentrations in Companion Bitches 2. To Confirm the Accuracy of the Prediction of Parturition Day)

  • 이주환;손창호
    • 한국임상수의학회지
    • /
    • 제37권6호
    • /
    • pp.305-310
    • /
    • 2020
  • To confirm the accuracy of the prediction of parturition day, the actual parturition days were compared with each day of the prediction of parturition day (n = 80). The accuracy of the prediction of parturition day was 80.0% (64/80) with a precision of ± 2 days from the first day of estrus after the first vaginal discharge, 97.5% (78/80) from the day when plasma progesterone concentrations increase above 4.0 ng/ml, and 72.5% (58/80) from the first day of diestrus, respectively. The accuracy of the prediction of parturition day by plasma progesterone concentration was higher than that by the first day of estrus and diestrus after the first vaginal discharge. These results indicated that the determination of plasma progesterone concentrations at estrus were a useful method for estimating of parturition day and for the reproductive management in pregnant bitches.

Progesterone EIA-kit에 의한 유우(乳牛)의 임신진단(姙娠診斷)에 관한 연구(硏究) (Studies on the early diagnosis of pregnancy of dairy cows by EIA-kit of progesterone in milk)

  • 김민규;신현주;이만휘;이명헌;김상근
    • 대한수의학회지
    • /
    • 제30권1호
    • /
    • pp.35-40
    • /
    • 1990
  • This study was carried out to evaluate khe accuracy of the early diagnosis of pregnancy in dairy cows using EIK-kit of progesterone in milk and to compare the progesterone concentrations in milk with those assayed by radioimmunoassay. 1. The progesterone concentrations of the pregnant cows($2.07{\pm}0.54ng/ml$) were significantly higher than those of non-pregnant cows($1.04{\pm}0.19ng/ml$), and thereafter began to inerease and maintained high levels. 2. During 20 to 22 days after artificial insemination, the accuracy of pregnancy diagnosis from EIA-kit of progesterone were 93.7% for non-pregnant cows, and 91.6% for pregnant cows. 3. During 20 to 22 days after artificial insemination, the accuracy of pregnancy diagnosis from milk progesterone concentrations were 100% for non-pregnant cows (<2.7ng/ml), and 92.9% for pregnant cows($${\geq_-}3.2ng/ml$$). The average overall accuracy of prediction for pregnant and non-pregnant cows were 96.5%. 4. Accordingly, the pregnancy diagnosis from EIA-kit of progesterone is thought to be recommendable because this early diagnostic means are simple with accurate result.

  • PDF

Roles of Gonadal Steroids on Exocrine Secretion of Isolated Perfused Rat Pancreas

  • Park, Hyung-Seo;Kim, Se-Hoon;Park, Hyoung-Jin;Lee, Mee-Young;Han, Young-Hee
    • The Korean Journal of Physiology and Pharmacology
    • /
    • 제7권4호
    • /
    • pp.217-221
    • /
    • 2003
  • To clarify the roles of gonadal steroids on pancreatic exocrine secretion, effects of progesterone and estradiol-$17{\beta}$ on spontaneous and secretagogue-induced exocrine response of isolated perfused rat pancreas were investigated. Intra-arterial infusion of progesterone resulted in significant increase of the spontaneous pancreatic fluid and amylase secretion dose-dependently. However, estradiol-$17{\beta}$ did not exert any influence on spontaneous pancreatic exocrine secretion. Exogenous secretin, cholecystokinin (CCK), and acetylcholine markedly stimulated pancreatic fluid and amylase secretion. Progesterone initially enhanced secretin-induced amylase secretion, but this stimulatory response declined thereafter to basal value. Moreover, secretin-induced fluid secretion was not affected by infusion of progesterone. Therefore, initial increase of secretion-induced amylase secretion by progesterone seems to be a non-specific action by washout effect of secretin. Estradiol-$17{\beta}$ failed to change the secretin-induced fluid and amylase secretion. Both progesterone and estradiol-$17{\beta}$ did not exert any influence on CCK-induced fluid and amylase secretion. Acetylcholine-induced exocrine secretion of isolated perfused pancreas also was not affected by intra-arterial infusion of progesterone or estradiol-$17{\beta}$. It is concluded from the above results that progesterone could enhance the spontaneous pancreatic fluid and amylase secretion of isolated perfused rat pancreas through non-genomic shortterm action, and that these effects could be masked by more potent stimulants such as secretin, CCK, and acetylcholine.

한우에서 rbST 처리가 Progesterone 농도와 황체의 크기 및 수태율에 미치는 영향 (Effect of Recombinant Bovine Somatotropin (rbST) Treatment on Concentration of Progesterone, Volume of Luteal Tissue and Pregnancy Rate in Hanwoo)

  • 정세환;공일근
    • 한국가축번식학회지
    • /
    • 제27권3호
    • /
    • pp.269-274
    • /
    • 2003
  • 본 연구는 과배란처리에 의한 수정란이식 시 우수한 수정란을 다량 확보하고 이식 후 수태율 향상을 위하여 수란우에 rbST처리가 수태율 및 progesterone 농도와 황체의 크기에 어떠한 영향을 미치는가를 조사하고자 실시하였다. 공란우는 Folltropin-V와 PGF$_2$$\alpha$를 이용하여 과배란처리를 유도하여 12시간 간격으로 2 straw씩 3회 인공수정을 실시하였다. 공란우와 수란우는 대조구와 rbST 처리구로 구분하였으며, rbST (500 mg)처리는 발정발현 후 미근부에 근육 주사하였다. 과배란처리된 공란우의 수정란채란은 수정 후 7∼8일째에 비외과적인 방법으로 실시하였다. 수정란이식 후 수태율은 rbST 처리구에서 대조구보다 유의적으로 높았다 (64.0 vs. 47.1%; p<0.05). 채취한 혈액의 progesterone 농도 분석과 황체의 단면적를 조사한 결과 progesterone 농도는 대조구와 rbST 처리구에서 6일 동안 차이가 없었지만, 6일 이후부터 차이가 나타났으며 황체크기 역시 rbST 처리구에서 대조구에 비해 높게 나타났다. 본 연구결과에서 rbST처리는 이식 후 수태율의 향상시킬 수 있을 것으로 판단된다.