• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.5
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• pp.620-628
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• 1996

To avoid the self-agglutination of Staphylococcus aureus sensitized with rabbit antibody in the absence of antigen, we determined the optimum concentration of rabbit antibody for sensitization. It was analyzed by using three different kinds of S. aureus strains at various concentraions of antibody. The optimized coagglutination test using the S. aureus sensitized with rabbit antibody was applied to the diagnosis of edwardsiellosis in field and in laboratory. The presence of E. tarda as low as $10\;{\mu}g/ml$ was detected by this method. Moreover, it showed good coagglutination results against several different forms of antigens such as FKC, EDTA or heat extracted antigen of E. tarda. E. tarda strains, isolated from the flounders suffering from edwardsiellosis in fields, showed some cross-reactions to the E. tarda 219 analyzed by both agglutination and coagglutination test with rabbit anti-E, tarda 219 antibody. The degree of cross-reactions analyzed was enough to apply the coagglutination test for the diagnosis of edwardsiellosis in field. Thus, even 1,000 fold diluted tissue homogenate of infected flounder naturally contained enough E. tarda as an antigen to show good coagglutination with S. aueus sensitized with rabbit anti-E, tarda 219 antibody. The successful application of this method to the homogenate and heat extract of tissues from naturally or artificially infected flounder or tilapia preyed that coagglutination test was a simple and rapid reliable dignostic technique suitable for using in laboratory and field without any special equipments.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.10
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• pp.448-453
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• 2017

The purpose of this study was to compare the marginal discrepancy of coping crowns according to manufacturing method and materials by using the CAD/CAM system to compare the optimum manufacturing method and materials. The coping crowns were fabricated by using manufacturing methods and materials, and marginal fit was measured using the replica technique. For comparative analysis of manufacturing methods and materials, analysis of variance was performed. One-way ANOVA was performed to compare and analyze different mean values. For the milling method of the alloy, 114.6 was obtained for the MM group, 111.4 for the MS group, 67.2 for the MSC group, and 50.9 for the MSS group, respectively, using the milling/sintering method. In the milling/sintering method of ceramic, 35.6 and 36.3, respectively, were obtained for the SLME group and SLMR group, respectively. However,there was a significant difference according to the material. The milling/sintering method of the ceramic material gave the lowest value, and it was verified by the optimal method. However, marginal discrepancy of coping crowns fabricated using the CAD/CAM system is applicable to the clinic since it corresponds to acceptable numerical values in clinical practice.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.7
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• pp.942-948
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• 2005

New polymorphism of major histocompatibility complex B-G genes was investigated by amplification and digestion of a 401bp fragment including intron 1 and exon 2 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique with two restriction enzymes of Msp I and Tas I in eight breeds of Chinese indigenous chickens and one exotic breed. In the fragment region of the gene, three novel single nucleotide polymorphisms (SNPs) were detected at the two restriction sites. We found the transition of two nucleotides of A294G and T295C occurred at Tas I restriction site, and consequently led to a non-synonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variable-region-like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that a single mutation of A294G occurring at the site, also caused an identical substitution of amino acid, asparagine 54-to-serine, to that we described previously. And the transversion of G319C at Msp I site led to a non-synonymous substitution, glutamine 62-to-histidine. The new alleles and allele frequencies identified by the PCR-RFLP method with the two enzymes were characterized, of which the allele A and B frequencies at Msp I and Tas I loci were given disequilibrium distribution either in the eight Chinese local breeds or in the exotic breed. By comparison, allele A at Msp I locus tended to be dominant, while, the allele B at Tas I locus tended to be dominant in all of the breeds analyzed. In Tibetan chickens, the preliminary association analysis revealed that no significant difference was observed between the different genotypes identified at the Msp I and Tas I loci and the laying performance traits, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.11
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• pp.1527-1533
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• 2001

Garole is a prolific, rare, less known and small size Indian sheep breed found in low and humid Sunderban region of West Bengal. Although information on stored Garole ram liquid semen upto 24 h is available, but there is a need to further investigate the short-term and long-term preservability of Garole ram semen for extensive utilization of this valuable germplasm by artificial insemination. The aim of the present study was to apply computer-assisted sperm analysis technique for assessing the motion characteristics of Garole ram semen stored (i) in liquid state at refrigeration temperature for short-term preservation upto 48 h and (ii) in frozen state at $-196^{\circ}C$ for long-term preservation after packaging in mini straws. Short-term preservation had a significant effect on motility (p<0.01) as the motility progressively decreased from 90.1% at 0 h to 85.5% and 73.2% after 24 and 48 h of storage, respectively. Although the decline in rapid moving sperms was also significant (p<0.01) on storage but the decrease was more pronounced at 48 h as compared to 24 h of storage period. Storage of chilled semen had also a significant effect on % linearity (p<0.05), % straightness (p<0.01), sperm velocities (p<0.01), amplitude of lateral head displacement (p<0.01) and beat frequency (pO.Ol) of spermatozoa. The replication had a significant effect for all the variables except average path and straight line velocity. However, the interactions of short-term storage and replication were non-significant for most of the variables except % of medium moving sperms, sperm velocities and beat frequency. On long-term preservation of Garole ram spermatozoa under controlled conditions the mean post-thaw recovery of 70.4 and 71.4% motile spermatozoa was achieved having 48.8 and 48.9% of rapidly motile spermatozoa, respectively in both the replicates. The effect of replication on cryopreservation was significant (p<0.05) on amplitude of lateral head displacement and beat frequency, but there was no significant effect on motility, rapidly motile spermatozoa, linearity, straightness and sperm velocities of frozen-thawed spermatozoa. It can be concluded from these results that an average 70% motility can be achieved on storage of Garole ram semen in chilled liquid state upto 48 h or in liquid nitrogen after freezing under controlled conditions in straws. However, further studies are required to evaluate the fertility of short-term and long-term preserved Garole ram semen for extensive use of this prolific sheep breed.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.2
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• pp.280-285
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• 2006

The current study was conducted to investigate the relationship between stress related gene and meat quality in pigs. A total number of 212 three-way cross bred (Landrace-$Yorkshire{\times}Duroc$) and 38 Duroc were sampled from the Korean pig industry to determine genotype frequency of porcine stress syndrome (PSS) and heat shock protein 70 kDa (HSP70) genes and their relationship with carcass traits and longissimus meat quality. Screen of HSP70 was performed by the single strand conformation polymorphism (SSCP) technique. Based on the analysis of restriction fragment length polymorphism (RFLP) in ryanodine receptor 1 (RYR1) gene, genetic disorder of PSS was related to a mutation at $18,168^{th}$ (C to T) of exon 17. There was no significant difference in ultimate meat pH and backfat thickness between HSP70 K1-AA type and -BB type in pure Duroc breed. In Landrace-$Yorkshire{\times}Duroc$ (L-$Y{\times}D$) cross bred pig, our results indicated that HSP70 derivate type in Duroc had a limited effect on backfat thickness, but L-$Y{\times}D$ type had a noticeable linkage with HSP70 K1-AA and K3-AB. This tendency was also observed in hot carcass weight where HSP70 K1-AA and K3-AB resulted in heavier weight with 86.3 kg compared to HSP70 K1-AB and K3-BB of 74.3 kg. Results imply that stress related HSP70 genotype has a potential association with backfat thickness and carcass weight.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.11
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• pp.1563-1567
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• 2017

Objective: The influence of substituting steam-flaked corn (SFC) for dry rolled corn (DRC) on feedlot cattle growth performance and dietary net energy when cattle are allowed either ad libitum or 2-h restricted access to the finishing diet was evaluated. Methods: Treatment effects were tested using 96 crossbred steers ($251{\pm}2kg$) during the initial 56 d of the finishing phase. Cattle were blocked by weight and randomly assigned within blocks to 16 pens (4 pens/treatment). Bunk space was sufficient (41 cm/head) to allow all steers access to the feed bunk at the same time. Treatments consisted of two finishing diets containing (dry matter basis) 77.1% corn grain processed by dry rolling (density = 0.50 kg/L) or steam flaking (density = 0.36 kg/L). Cattle were fed twice daily at 06:00 and 14:00 h, allowing for approximately 5% residual. In the case of restricted feeding, steers were allowed access to feeders for 1 h following each feeding, after which residual feed was withdrawn. Results: There were no treatment interactions on dry matter intake (DMI), average daily gain (ADG), gain efficiency (G:F), or dietary net energy (NE). Restricting feed access time reduced (p<0.01) feed intake, and hence, ADG. Substitution of SFC for DRC increased (p<0.01) ADG, feed efficiency (G:F), and estimated dietary NE, without affecting DMI. Based on tabular net energy of maintenance ($NE_m$) value (2.18 Mcal/kg) for DRC, the estimated $NE_m$ value for SFC using the replacement technique, averaged 2.44 Mcal/kg; an improvement of 10.7%. The ratio of observed-to-expected dietary NE was not affected by feed access time. Conclusion: Substitution of SFC for DRC in finishing diets for feedlot cattle enhanced ADG, gain efficiency, and the NE value of the diet. Although restriction of feed access time depressed DMI and ADG, it did not affect the comparative benefit of steam flaking toward enhancement of ADG, G:F, and dietary NE.

• Conservation Science in Museum
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• v.22
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• pp.69-84
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• 2019

This study investigated the formal characteristics and chemical compositions of eighty beads excavated from a Silla tomb complex at Deokcheon-ri, Uljin in order to reveal the methods of production and the characteristics of the raw materials involved, thereby illuminating characteristics of the relics from each tomb. The beads excavated from these Silla tombs include one curved bead, one tubular bead, and 78 globular beads. Among them, the curved and tubular beads and three globular beads were identified to be made of minerals, and the remaining 75 beads are made of glass. Most of the glass beads are thought to have been produced using the drawing technique. The characteristics of the beads were classified according to their site of excavation. The globular beads from Tomb No. 34 belong to the lead-barium glass and potash glass groups, indicating that these beads were produced at a relatively earlier period in time. The glass beads excavated from the tombs in the northern portion of the complex differ in color and chemical composition from those in the southern portion, which indicates the character that each group of beads had different aspect according to site location. Considering that the excavated beads were mainly from fifth- and sixth-century tombs, it is likely that certain groups of people living around Uljin used and buried beads in their tombs. As the governance of Silla became more centralized in the sixth century, the use of beads for burial purpose appears to have come to an end in this region.

• Korean Journal of Construction Engineering and Management
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• v.16 no.3
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• pp.91-100
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• 2015

An increase in energy such as natural gas, coal, oil, has occurred to a large amounts of environment impact emissions, it is necessary to reduce in the construction industry for the energy consumption. To encourage remodeling project in developed countries of the majority, on the basis of this, remodeling project in the construction industry has grown to a large amount. Results of analysis of the research related to the advanced remodeling, analysis of the economic validity in accordance with the production and process and building elapsed years of selection alternative of remodeling there has been a problem that has not been properly reflected. In this study, a decision support model that can simultaneously choose the most cost-effective and energy-efficiency alternative. Developed process model, generates a "Remodeling Solution" that combines the renewable energy equipment and envelope system, energy performance evaluation of the application of international standards(ISO-13790, DIN V 18599), perform the economic evaluation through LCCA(Life Cycle Cost Analysis) technique, circulated evaluation and configured to output the optimal Remodeling Solution. The results of applying the model developed in the case, it was confirmed that it is possible to select a choice of cost-effective energy-saving alternative. Then, developed model through this study, it is expected to be able to help highly effective remodeling alternative to selecting by decision-makers.

• The KIPS Transactions:PartD
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• v.17D no.5
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• pp.371-382
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• 2010

One of the advantages of the mobile internet is that it is accessible everywhere. However, producing the mobile internet contents is hard because the standards of the contents are different depending on the telecommunications companies and the mobile phone terminals. Because of this, mobile contents production has not been activated and profit models are rarely found. In order to vitalize the mobile contents, we developed the Mobile UCC (User Created Contents) Phone Page Service with which general users can produce and provide the mobile phone pages easily. In addition, we began to provide advertisement on the UCC phone pages as a profit model. However, as mentioned already, the contents that meet each of the standards should be prepared in advance to be compatible with the different standards. For this reason, only one advertisement could be provided on one UCC phone page. Previous studies were focused on the standardization of different standards by integrating them. Though the standardization may be applicable to the mobile phones that will be newly released, it is hardly applied to the conventional mobile phones. To solve the abovementioned problems and improve the drawbacks of the conventional studies, we proposed in this article the technology to synthesize the phone page contents and independent advertisement at the moment when the UCC phone page prepared by the users is provided in real time. This technology allows to provide various advertisement on one UCC phone page and administrate the start, end and frequency of specific advertisements. This method was applied in this study to vitalize the mobile contents and improve the profit model.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.12
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• pp.613-619
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• 2017

Recently, the development and production of weapons systems for military products have been increasing. Therefore, there is a need for an efficient quality control method suitable for limited resources and manpower for the manufactured military products. Military munitions are characteristically developed and produced as business contract units. In addition, business and quality control are done by contract. For this reason, many contracts are concluded in one producer and similar products are often produced. Business and quality control also require extensive manpower and effort. If a company produces several similar products, a more efficient management approach is required. This study therefore examines these problems in an attempt to present solutions by analyzing the types of munitions contracts and the characteristics of products produced by one company. Based on the results, a method is proposed to manage the contract-specific management methods by company, that is, by business site. The method is applied to an engine manufacturing company and the integrated management plan is verified and its effectiveness confirmed. The effect of facility-wide method was a reduction of QA manpower and an improvement in maintaining quality assurance and stability through management of the workforce and contract grouping method.