• Journal of Bio-Environment Control
• /
• v.13 no.4
• /
• pp.258-265
• /
• 2004

The experiment was conducted to investigate the effect of EC and pH of nutrient solution on the growth and quality of the single-stemmed rose grown in a rose factory installed with hydroponic system. The growth and quality of the single-stemmed rose were not significantly different from each other with the EC of the nutrient solutions $1.0\~3.5dS{\cdot}m^{-1}$, which resulted in concluding high concentration of the nutrient solution was not necessary. The optimum range of the EC for single-stemmed rose was $1.5\~2.0dS{\cdot}m^{-1}$ considering plant growth, photosynthetic and transpiration rates. The optimum range of the pH for good plant growth without any visible physiological disorder was $4.0\~6.0$. Therefore, to keep the pH of the nutrient solution for rose low compared to other plants was beneficial for plant growth and uptake of the mineral ions.

• Journal of the Korean Institute of Gas
• /
• v.24 no.5
• /
• pp.56-64
• /
• 2020

This study presents the hydrate induction time of PVCap according to subcooling temperature, salt concentration, and MEG concentration in order to analyze the inhibition effect of PVCap in various production environments of offshore gas fields. A high-pressure hydrate generator was made for the hydrate formation experiments. It was verified that the apparatus had sufficient reliability by comparing the results of hydrate equilibrium conditions and induction time from the apparatus with published reference data. As the subcooling temperature increased from 6.1℃ to 12.1℃, the induction time of PVCap concentration of 0.1~1 wt% decreased. When the salt concentration increased from 3 wt% to 7 wt%, the induction time was reduced by up to 78% under the condition of 0.5 wt% PVCap due to polymer structure degradation by salt effect. In the case of HHI (hybrid hydrate inhibitor) made by mixing MEG 10 wt% and PVCap, the change in induction time was not large compared to PVCap 1 wt% due to the under-inhibition effect. On the other hand, the hydrate inhibition efficiency of HHI with MEG 20wt% increased 1.7 times compared to PVCap.

• The Korean Journal of Nuclear Medicine
• /
• v.27 no.2
• /
• pp.223-232
• /
• 1993

Technetium labeled isonitrile analogues are widely used as myocardial perfusion imaging agents. We synthesized and characterized a new isonitrile compound, ethyl 3-isocyanobutyrate(EIB). Proton and $^{13}C$ NMR spectroscopy and thin layer chromatography with a $C_{18}$ coat was performed. EIB was easily labeled with $^{99m}TcO_4^-$- with sodium dithionite. The labeling efficiency measured by RP-HPLC was over 95%. The labeled product was stable with dilution in normal saline and with prolonged incubation at room temperature. There was no formation of secondary products or free $^{99m}TcO_4^-$. In vivo kinetics study of $^{99m}Tc$ (I) labeled EIB in rabbits showed adequate myocardial uptake, good contrast against lung background, and relatively rapid liver clearance. The heart to lung ratio was over 2.5 and the heart to liver ratio was approximately from 0.4 to 5 at 60 minutes post injection. Hepatic clearance of $^{99m}Tc-MIBI$ was faster ($t_{1/2}$=6 minutes) than that of $^{99m}Tc-MIBI$. In vivo kinetics observed in dog was similar to that in rabbit but there was faster gallbladder filling, and thus lower liver background. SPECT imaging of the canine myocardium showed favorable imaging characteristics. However, biodistribution in mice demonstrated a myocardial % injected dose/organ of less than 0.1%. This was thought to be due to interspecies difference in plasma esterase activity. In human plasma, $^{99m}Tc$ ( I ) labeled EIB was stable for at least 2 hours, without production of secondary products by HPLC. We conclude that ethyl 3-isocyanobutyrate may be a potential new myocardial perfusion imaging agent and deserves further investigation as to its usefulness for clinical use.

• Journal of Plant Biotechnology
• /
• v.32 no.4
• /
• pp.257-262
• /
• 2005

Agrobacterium tumefaciens-mediated cotyledonary-node explants transformation was used to produce transgenic melon. Cotyledonary-node explants of melon (Cucumis melo L. cv. Super VIP) were co-cultivated with Agrobacterium strains (LBA4404, GV3101, EHA101) containing the binary vector (pPTN289) carrying with CaMV 35S promoter-gus gene as reporter gene and NOS promoter-bar gene conferring resistance to glufosinate (herbicide Basta) as selective agent, and the binary vector (pPTN290) carrying with Ubiquitin promoter-GUS gene and NOS promoter-nptll gene conferring resistance to paromomycin as selective agent, respectively. The maximum transformation efficiency (0.12%) was only obtained from the cotyledonary-node explants co-cultivated with EHA101 strain (pPTN289) on selection medium with 5 mg/L glufosinate and not produced a transgenic melon from the cotyledon or cotyledonary-node co-cultivated with other strains. Finally, five plants transformed showed the resistance in glufosinate antibiotic and the GUS positive response in leaf ($T_0$), flower ($T_0$), seeds ($T_1$) and plantlet ($T_1$). Southern blot analysis revealed that the gus gene integrated into each genome of transgenic melon.

• Journal of Plant Biotechnology
• /
• v.31 no.1
• /
• pp.31-35
• /
• 2004

In order to develop an efficient micropropagation technique for an endangered species, Stellera rosea N., stem node cultures were conducted on MS medium supplemented with cytokinins. Generally, BA was better than zeatin on shoot proliferation from stem nodes, whereas zeatin showed more effective on shoot elongation. In vitro rooting of shoots was achieved by application of an auxin pre-culturing method. Overall rooting rate was relatively low and differed depending on the culture period. Pre-culturing of shoots for 15 days at 1.0mg/L IBA revealed a slightly better rooting efficiency reaching 30％ rooting rate than NAA. Root induction rate by NAA also varied with concentration of NAA and culture periods. Total 51％ of the rooted plantlets survived on artificial soil mixture and grew normally without any distinct morphological variation. The results suggest that the endangered Stetllera plants are propagated via in vitro culture system, but still need to more study for the improvement of rooting and acclimatization of the plantlets in soil.

• Journal of Society of Preventive Korean Medicine
• /
• v.3 no.2
• /
• pp.55-77
• /
• 1999

Oriental Medical Service established foundation that Oriental medical clinic can take part in industrial health service by being specified industrial calamity medical treatment organ, but research which develope program that Oriental Medical Service approach on the occupational disease and industrial health only dealt in the Western Medical System was insufficient. So we studied on theoretical foundation and basic bearing about .program of oriental medical health examination in the workplace, it is based on explanation and assessment reformed by western medicine. We reached the conclusion as follows, 1. We must reconsider the concept that mind of Oriental medicine is preventive medicine, and assess positively result of social medicine. 2. Importance of industrial health is being enlarged in the category of health care. 3. Western medical health in including industrial health have faced much problems at this time, we can search by alternative proposal grafting on Oriental Medicine. 4. Oriental medical participation in the industrial health have various benefits at side satisfaction of workers, effect of prevention, efficiency of cost, and increasing rate of medical treatment and return to workplace of workers with industrial calamity. 5. We must consider Oriental Medical Health Examination as one way of health management program of workers, not as formality. 6. The advantage of Oriental Medical Health Examination can increase ability of individual health management in preventive medicine and the production in workers' health management.

• Korean Journal of Soil Science and Fertilizer
• /
• v.45 no.6
• /
• pp.861-866
• /
• 2012

Water is the most important resource for the potato cultivation, especially to get the maximum water use efficiency and yield of potato, Water has to be applied moderately based on the water requirement of the potato. Crop water requirement (WR) is a function of the Potential evapo-transpiration(PET) and Crop coefficient (Kc). PET can be estimated by the climate data measured at the weather station in the production region. Kc was measured by the NIAST (RDA) through Lysimeter experiments. In this study, the growth stage of potato was divided as four (G-1 : Apr. 1~Apr. 15, G-2 : Apr. 16~May. 10, G-3 : May. 11~May. 31, G4 : Jun. 1~Jun. 15). The average PET during potato growing season of the 45 areas was $2.95mm\;day^{-1}$. The most water requirement was the G-3 stage among the potato growth stage. The MWR (Mean water requirement) according to growth stage was 1.0~1.2 (average 1.1), 1.5~1.8 (average 1.6), 1.9~2.2 (average 2.0) and 1.7~2.1 (average 1.8) mm $day^{-1}$, in the G-1, G-2, G-3 and G-4 stage, respectively. The TWR (Total water requirement) according to growth stage was 18.0~22.1 (average 19.3), 50.6~66.6 (average 56.3), 63.5~88.2 (average 72.4) and 38.3~54.5 (average 44) mm, in the G-1, G-2, G-3 and G-4 stage, respectively.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.30 no.12 s.159
• /
• pp.1683-1689
• /
• 2006

Precedent researches on lyocell are mostly on lyocell structure, characteristics, physical properties by finishing and dyeability, and the like; there are little cases of researches on interlining for lyocell clothes production. To serve as a base information helpful to select fusible interlining and improve the efficiency of sewing process considering the characteristics of lyocell, this study performed t-est, F-test toward to the difference in the appearance-related properties and the mechanical properties of lyocell-interlining bonded fabric in accordance with the characteristics of fusible interlining and lyocell face fabric. This study reached following conclusions. Drapability and Crease recovery of lyocell-interlining bonded fabric are influenced by the structure of fusible interlining; stiffness of lyocell-interlining bonded fabric, by the structure of fusible interlining and the yam number of lyocell face fabric. WT is influenced by the mixture rate and yam number of lyocell face fabric. RT is influenced by the yam number of lyocell face fabric; G, 2HG, 2HG5, by the structure(woven and non-woven) of fusible interlining; B, HB, by the weight, structure(woven and non-woven) of fusible interlining, and yam number of lyocell face fabric; WC, by the weight, structure of fusible interlining; RC, by the structure of fusible interlining; thickness, by the weight of fusible interlining and the yarn number of lyocell face fabric; weight, by mixture rate and yam number of lyocell face fabric.

• Journal of Microbiology and Biotechnology
• /
• v.14 no.6
• /
• pp.1239-1248
• /
• 2004

The methylotrophic yeast Hansenula polymorpha has been extensively studied as a model organism for methanol metabolism and peroxisome biogenesis. Recently, this yeast has also attracted attention as a promising host organism for recombinant protein production. Here, we describe the fabrication and evaluation of a DNA chip spotted with 382 open reading frames (ORFs) of H. polymorpha. Each ORF was PCR-amplified using gene-specific primer sets, of which the forward primers had 5'-aminolink. The PCR products were printed in duplicate onto the aldehyde-coated slide glasses to link only the coding strands to the surface of the slide via covalent coupling between amine and aldehyde groups. With the partial genome DNA chip, we compared efficiency of direct and indirect cDNA target labeling methods, and found that the indirect method, using fluorescent-labeled dendrimers, generated a higher hybridization signal-to-noise ratio than the direct method, using cDNA targets labeled by incorporation of fluorescence-labeled nucIeotides during reverse transcription. In addition, to assess the quality of this DNA chip, we analyzed the expression profiles of H. polymorpha cells grown on different carbon sources, such as glucose and methanol, and also those of cells treated with the superoxide­generating drug, menadione. The profiles obtained showed a high-level induction of a set of ORFs involved in methanol metabolism and oxidative stress response in the presence of methanol and menadione, respectively. The results demonstrate the sensitivity and reliability of our arrays to analyze global gene expression changes of H. polymorpha under defined environmental conditions.

• Cartoon and Animation Studies
• /
• s.14
• /
• pp.151-164
• /
• 2008

Cross platform game has brought about the expansion of game market, which results in technology innovation overcoming the limit of game consumption. The new model integrates both off and online game services. Gamers can now enjoy game service regardless of age, time, and space. If the technology evolution model of digital contents like cross-platform game engine can provide contents for several platform at the same time, the interactive service can be utilized into maximum level. It is also necessary to allocate, switch data as well as to innovate the transmission technology of data according to each platform. Providing the same contents for several platform as many as possible can be the most suitable strategy to enhance the efficiency and profits. However if the interactive service can be accomplished completely, the development of data switching technology and distribution should be made. To be a leader in the next digital contents market, one should develop the network engine technology which can embody the optimization of consumption in the interactive network service.