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http://dx.doi.org/10.5010/JPB.2004.31.1.031

Micropropagation of an Endangered Species, Stellera rosea Nakai by Tissue Culture  

Han, Mu-Seok (Warm-temperate Forest Research Center, Korea Forest Research Institute)
Moon, Heung-Kyu (Division of Biotechnology, Korea Forest Research Institute)
Kang, Young-Jae (Warm-temperate Forest Research Center, Korea Forest Research Inst)
Kim, Won-Woo (Warm-temperate Forest Research Center, Korea Forest Research Inst)
Kang, Byung-Seo (Warm-temperate Forest Research Center, Korea Forest Research Inst)
Byun, Kwang-Ok (Warm-temperate Forest Research Center, Korea Forest Research Inst)
Publication Information
Journal of Plant Biotechnology / v.31, no.1, 2004 , pp. 31-35 More about this Journal
Abstract
In order to develop an efficient micropropagation technique for an endangered species, Stellera rosea N., stem node cultures were conducted on MS medium supplemented with cytokinins. Generally, BA was better than zeatin on shoot proliferation from stem nodes, whereas zeatin showed more effective on shoot elongation. In vitro rooting of shoots was achieved by application of an auxin pre-culturing method. Overall rooting rate was relatively low and differed depending on the culture period. Pre-culturing of shoots for 15 days at 1.0mg/L IBA revealed a slightly better rooting efficiency reaching 30％ rooting rate than NAA. Root induction rate by NAA also varied with concentration of NAA and culture periods. Total 51％ of the rooted plantlets survived on artificial soil mixture and grew normally without any distinct morphological variation. The results suggest that the endangered Stetllera plants are propagated via in vitro culture system, but still need to more study for the improvement of rooting and acclimatization of the plantlets in soil.
Keywords
Shoot proliferation and rooting; auxin pre-culturing for rooting; plantlets production;
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1 Ramirez-Malagon R, Borodanenko A, Barrera-Querra JL, Ochoa Alejo N (1997) Micropropagation for fi-aser photinia (Photirtia $\times$ frasen). Plant Cell Tiss Org Cult 48: 219-222   DOI   ScienceOn
2 Sha L, McCown BH, Peterson LA (1985) Occurrence and cause of shoot-tip necrosis in shoot culture. J Amer Soc Hort Sci 110: 631- 634
3 Siril EA, Dhar U (1997) Micropropagation of mature Chinese tallow tree (Sapium sebiferum Roxb.). Plant Cell Rep 16: 637-640   DOI   ScienceOn
4 Youn Y, Lee SK, Park JI (1992) In vitro propagation of a rare species-Berchemia berchemiaefotia. Res Rep For Gen Res Inst Kor 28: 63-67
5 Agarwal B, Singh U, Banerjee M (1992) In vitro clonal propagation of tea (CameIlia sinensis L.O. Kuntze). Plant Cell Tiss Org Cult 30:1-5   DOI
6 Barghchi (1988) Micropropagation of Alnus cordata (Loisel). Plant Cell Tiss Org Cult 15:233-244   DOI
7 Chungbu Forest Experiment Station (1997) Illustrated rare and endangered species in Korea. Korea Forest Research Institute, pp 255
8 De Klerk G J (1995) Hormone requirements during the successive phases of rooting of Malus microcuttings. In: Terzi M, Cella R, Falavigna A (eds), Current Issues in Plant Molecular and Cellular Biology, Vol 22. KIuwer Aca Pub, pp 111-116
9 Krogstrup P, Baldursson S, Norgarrd JV (1992) Ex situ genetic conservation by use of tissue culture. Opera Bot 113:49-53Lee TB (1993) Illustrated flora of Korea. Hyangmoonsa, Seoul, pp 561
10 Cuenca S, Amo-Marco J B, Parra R (1999) Micropropagation from inflorescence stems of the Spanish endemic plant Centaurea paui Loscos ex Willk. (Compositae). Plant Cell Rep 18:674-679   DOI   ScienceOn
11 Fay MF (1994) In what situation is in vitro culture appropriate to plant conservation? Biodiv Conserv 3:176-183   DOI
12 Hammatt N, Evans PK (1985) The in vitro propagation of an endangered species : Centaurea junoniana Svent. (Compositae). J Hort Sci Sci 60: 93-97
13 Iriondo JM, Perez C (1996) Micropropagation and ih vitro storage of Centaurium rigualii Esteve (Gentianaceae). Israel J Plant Sci 44: 115-123   DOI   ScienceOn
14 Lied$\dot o$ MD, Crespo MB, Amo-Marco JB (1995) In vitro multiplication of VeIla lucentina MB Crespo (Brassicaceae), a threatened spanish endemic species. In Vitro CelI Dev Biol 31: 199-201   DOI
15 Moon HK, Suk GY, Kim SC (1997) Micropropagation of a rare species, Forsythia saxatillis N. through tissue culture. J Kor For Soc 86: 430-434
16 Moon HK, Suk GY, Kwon YJ, Son SH (1999) Micropropagation of a rare species, Abeliophyllum. disticchum Nakai. via axillary bud culture. Kor J PIant Tiss Cult 26: 133-136
17 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497   DOI