• KSBB Journal
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• v.13 no.4
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• pp.461-467
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• 1998

A fermentation system was supplemented with a device for the measurement of the durations of alkali pump feeding for automatic pH control and an A/D convertor for precise monitoring of pH value by computer. A software program was developed to measure buffer capacities from the pH signal and the pH control signal during fermentation. By measuring the buffer capacity on-line, levels of acetic acid were estimated by a software sensor using pH signal in a fermentation process of E.coli growing in a minimal medium. The measured values of acetic acid showed correlation to those of estimated by the software sensor. Lacitic acid production was also successfully estimated by the values of buffer capacities measured on-line.

• Mycobiology
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• v.44 no.2
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• pp.105-111
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• 2016

Paclitaxel (taxol) has long been used as a potent anticancer agent for the treatment of many cancers. Ever since the fungal species Taxomyces andreanae was first shown to produce taxol in 1993, many endophytic fungal species have been recognized as taxol accumulators. In this study, we analyzed the taxol-producing capacity of different Colletotrichum spp. to determine the distribution of a taxol biosynthetic gene within this genus. Distribution of the taxadiene synthase (TS) gene, which cyclizes geranylgeranyl diphosphate to produce taxadiene, was analyzed in 12 Colletotrichum spp., of which 8 were found to contain the unique skeletal core structure of paclitaxel. However, distribution of the gene was not limited to closely related species. The production of taxol by Colletotrichum dematium, which causes pepper anthracnose, depended on the method in which the fungus was stored, with the highest production being in samples stored under mineral oil. Based on its distribution among Colletotrichum spp., the TS gene was either integrated into or deleted from the bacterial genome in a species-specific manner. In addition to their taxol-producing capacity, the simple genome structure and easy gene manipulation of these endophytic fungal species make them valuable resources for identifying genes in the taxol biosynthetic pathway.

• Journal of Korean Institute of Industrial Engineers
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• v.24 no.1
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• pp.157-165
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• 1998

This paper considers the single-product production and transportation problem with discrete time, dynamic demand and finite time horizon, an extension of classical dynamic lot-sizing model. In the model, multiple freight container types are allowed as the transportation mode and each order (product) placed in a period is shipped immediately by containers in the period. Moreover, each container has type-dependent carrying capacity restriction and at most one container type is allowed in each shipping period. The unit freight cost for each container type depends on the size of its carrying capacity. The total freight cost is proportional to the number of each container type employed. Such a freight cost is considered as another set-up cost. Also, it is assumed in the model that production and inventory cost functions are dynamically concave and backlogging is not allowed. The objective of this study is to determine the optimal production policy and the optimal transportation policy simultaneously that minimizes the total system cost (including production cost, inventory holding cost, and freight cost) to satisfy dynamic demands over a finite time horizon. In the analysis, the optimal solution properties are characterized, based on which a dynamic programming algorithm is derived. The solution algorithm is then illustrated with a numerical example.

• Microbiology and Biotechnology Letters
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• v.51 no.4
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• pp.484-499
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• 2023

Plant growth-promoting (PGP) bacteria can be used as bioresources to enhance phytoremediation through their PGP traits and pollutant removal capacity. In this study, 49 rhizobacteria were primarily isolated from the rhizosphere of tall fescue grown in diesel- and heavy metal-contaminated soil. Their biosurfactant production, phosphate (P) solubilization, and siderophore production were qualitatively and quantitatively evaluated to identify superior PGP bacteria. The optimal conditions for the growth of PGP bacteria and the stability of their PGP traits were a temperature of 35℃, a pH of 7, and 2 days of cultivation time. Four superior PGP bacteria (Pseudomonas sp. NL3, Bacillus sp. NL6, Bacillus sp. LBY14, and Priestia sp. TSY6) were finally selected. Pseudomonas sp. NL3 exhibited superior biosurfactant production and P solubilization. Bacillus sp. NL6 showed the highest P solubilization and superior production of biosurfactants and siderophores. Bacillus sp. LBY14 offered the best siderophore production and impressive P solubilization. Priestia sp. TSY6 had superior capacity for all three PGP traits. Through their secretion of beneficial PGP metabolites, the four bacteria isolated in this study have the potential for use in the phytoremediation of contaminated soil.

• Journal of the Korean Solar Energy Society
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• v.32 no.3
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• pp.33-41
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• 2012

As the wind farms in large scale demand enormous amount of construction cost, minimizing the economic burden is essential and also it is very important to measure the wind resources and forecast annual energy production correctly to judge the economic feasibility of the proposed site by way of installing a Met mast at or nearby the site. Wind resources were measured by installing a 80[m] high Met mast at WangdeungYeo Island to conduct the research incorporated in this paper and offshore wind farm was designed using WindPRO. Wind farm of 100[MW] was designed making use of 3 and 4.5[MW] wind generator at the place selected to compare their annual energy production and capacity factor applying the loss factor of 10[%] and 20[%] respectively to each farm. As a result, 336,599[MWh] was generated by applying 3[MW] wind generator while 358,565 [MWh] was produced by 4.5[MW] wind generator. Difference in the energy production by 3[MW] generator was 33,660 [MWh] according to the loss factor with the difference in its capacity factor by 3.8[%]. On the other hand, 23 units of 4.5 [MW] wind generators showed the difference of annual energy production by 35,857 [MWh] with 4.0[%] capacity factor difference.

• Journal of the Korea Society of Computer and Information
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• v.12 no.5
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• pp.273-284
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• 2007

This paper studies a periodic review inventory model with an e-MarketPlace transaction in reconfigurable manufacturing system(RMS). A decision maker can expand/reduce production capacity/quantities and/or replenish/dispose inventories from/to e-MarketPlace urgently to satisfy the stochastic demands. If inventories are replenished or disposed through e-MarketPlace, this leadtime is shorter than the production leadtime, but unit purchasing or selling cost is more expensive than that of expanding capacity or reducing production quantities respectively. Henceforth, trade-off on these alternatives is considered. In addition to this, in order to consider the economy of scale, our model includes the fixed cost for purchasing from e-MarketPlace and capacity expansion. We use dynamic programming and K convexity methods to characterize the nature of the optimal policy. Finally, We present the optimal inventory control policy which is composed by the combinations of a base stock and (s,S) type policy.

• Journal of Environmental Science International
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• v.8 no.5
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• pp.575-586
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• 1999

From the environmental aspects, primary productivity of phytoplankton plays the most improtant role in enhancement of marine culture oyster production. This study may be divided into two branches; one is estimation of maximum oyster meat production per unit facility(Carrying Capacity) under the present enviromental conditions in Kamak Bay, the other is improvement of carrying from increase of primary productivity by changing the environmental conditions that cause not ot form an unfavorable environment such as the formation of oxygen deficient water mass using the eco-hydrodynamic model. By simulation of three-dimensional hydrdynamic model and ecosystem model, the comparison between observed and computed data showed good agreement. The results of sensitivity analysis showed that phytoplankton maximum growth rate was the most important parameter for phytoplankton and dissolved oxygen. The estimation of mean primary productivity of Wonpo, Kamak, Pyongsa, and Kunnae culture grounds in Kamak Bay during culturing period were 3.73gC/$m^2$/d, 2.12gC/$m^2$/d, 1.98gC/$m^2$/d, and 1.26gC/$m^2$/d, respectively. Under condition not ot form the oxygen deficient water mass, four times increasing of pollutants loading as much as the present loading from river increased mean primary productivity of whole culture grounds to 4.02gC/$m^2$/d. Sediment N, P fluxes that allowed for 35% increasing from the present conditions increased mean primary productivity of whole culture grounds to 3.65gC/$m^2$/d. Finally, ten times increasing of boundary loadings from the present conditions increased mean primary productivity of whole culture grounds to 3.95gC/$m^2$/d. The maximum oyster meat production per year and that of unit facility in actual oyster culture grounds under the present conditions were 6,929ton and 0.93ton, respectively. This 0.93ton/unit facility is considered to be the carrying capacity in study area, and if the primary productivity is increased by changing the environmental conditions, oyster production can be increased.

• Animal Bioscience
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• v.34 no.5
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• pp.844-854
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• 2021

Objective: The potential of extra virgin olive oil (EVOO), betaine (BET), and ginger (GIN), as natural antioxidants, in reducing negative effects of heat stress on physiological responses, antioxidant capacity, semen quality and fertility of bucks under heat stress were investigated. Methods: Forty adult Animal Production Research Institute line rabbit bucks were distributed randomly into four experimental treatments of ten rabbits each. The first treatment was fed the commercial pellet diet (CPD) without supplementation and served as a control. The other three treatments were fed CPD supplemented with EVOO (300 mg), BET (1,000 mg), and GIN (200 mg) per kg diet for 3 consecutive months during the summer season. Results: Supplementation of EVOO, BET, or GIN improved (p<0.05) the sexual desire, progressive motility, vitality, intact acrosome and membrane integrity, sperm cell concentration, sperm outputs and fertility. Seminal plasma total proteins, globulin, total antioxidant capacity, glutathione and glutathione S-transferase, and initial fructose increased (p<0.05), while total lipids, aspartate and alanine aminotransferases and malondialdehyde decreased (p<0.05) compared with the control. In comparing the natural antioxidants treatments, GIN evoked the largest improvement. Conclusion: The inclusion of GIN (200 mg/kg diet) appeared to improve the sexual desire, semen quality and oxidative stress of bucks. This may be a beneficial supplement for the management of rabbit bucks used in natural mating or artificial insemination.

• Industrial Engineering and Management Systems
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• v.11 no.2
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• pp.170-175
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• 2012

In this paper, a procedure using a genetic algorithm (GA) and a heuristic local search (HLS) is proposed for solving facility rearrangement problem (FRP). FRP is a decision problem for stopping/running of facilities and integration of stopped facilities to running facilities to maximize the production capacity of running facilities under the cost constraint. FRP is formulated as an integer programming model for maximizing the total production capacity under the constraint of the total facility operating cost. In the cases of 90 percent of cost constraint and more than 20 facilities, the previous solving method was not effective. To find effective alternatives, this solving procedure using a GA and a HLS is developed. Stopping/running of facilities are searched by GA. The shifting the production operation of stopped facilities into running facilities is searched by HLS, and this local search is executed for one individual in this GA procedure. The effectiveness of the proposed procedure using a GA and HLS is demonstrated by numerical experiment.

• Journal of Korean Institute of Industrial Engineers
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• v.26 no.1
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• pp.17-26
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• 2000

This paper presents an integrated order scheduling method with the improved DRP concept for multi-echelon distribution system that has the constraint of limited production capacity of producers. The proposed method reflects the dynamic characteristics of inventory level changes in the regional and central distribution center. The simulation is done with two models : the traditional DRP method and the proposed method presented in this paper. From the results, the latter is more efficient than the former in cost, customer's service level as well as balanced production load on each producer.