• Reproductive and Developmental Biology
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• 제35권3호
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• pp.251-256
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• 2011

Human growth hormone (hGH), one of the most important hormones in medicine, is secreted from anterior pituitary gland. Its broad physiological function includes body growth, cell regeneration, increasement of muscle volume, bone density, body fat reduction, and so on. Due to the wide range of therapeutic effects, the hGH produced from E. coli has been commercialized already. In this study, we asked whether it is possible to produce recombinant hGH efficiently from various cultured mammalia cells. To meet this purpose, we chose a retrovirus vector system for transfer and expression of the hGH gene in various mammalian cells. Analyses of RT-PCR, ELISA, and Western blot to determine expression of the hGH gene showed the highest production of the hGH was determined from chicken embronic fibroblast (CEF) cells with the concentration of 8.58 ${\mu}g$/ml. The biological activity of the hGH was similar to the commercially available counterpart. These results suggest that mass production of hGH is possible not only in the E. coli but also in the various mammalian cells.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2005년도 춘계학술대회 논문집
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• pp.43-46
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• 2005

Micro molding technology is a promising mass production technology for polymer based microstructures. Mass production technologies such as the micro injection/compression molding, hot embossing, and micro reaction molding are already in use. In the present study, we have developed a numerical analysis system to simulate three-dimensional non-isothermal cavity filling for hot embossing, with a special emphasis on the free surface capturing. Precise free surface capturing has been successfully accomplished with the level set method, which is solved by means of the Runge-Kutta discontinuous Galerkin (RKDG) method. The RKDG method turns out to be excellent from the viewpoint of both numerical stability and accuracy of volume conservation. The Stokes equations are solved by the stabilized finite element method using the equal order tri-linear interpolation function. To prevent possible numerical oscillation in temperature Held we employ the streamline upwind Petrov-Galerkin (SUPG) method. With the developed code we investigated the detailed change of free surface shape in time during the mold filling. In the filling simulation of a simple rectangular cavity with repeating protruded parts, we find out that filling patterns are significantly influenced by the geometric characteristics such as the thickness of base plate and the aspect ratio and pitch of repeating microstructures. The numerical analysis system enables us to understand the basic flow and material deformation taking place during the cavity filling stage in microstructure fabrications.

• Asian-Australasian Journal of Animal Sciences
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• 제23권3호
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• pp.355-365
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• 2010

The aim of this study was to investigate the effect of supplemental recombinant bovine somatotropin (rbST) and cooling with misters and fans on renal function in relation to regulation of body fluids in different stages of lactation in crossbred Holstein cattle. Ten, 87.5% crossbred Holstein cattle were divided into two groups of 5 animals each, housing in a normal shaded barn (NS) and in a shaded barn with a mister-fans cooling system (MF). The experiment in each group was divided into 3 phases, early- (Day 75 postpartum), mid- (Day 135 postpartum), and late stage of lactation (Day 195 postpartum). The pre-treatment study was conducted on the starting day of each stage of lactation and the treatment study was performed after the end of the pre-treatment, during which the animal was injected with 500 mg of rbST (POSILAC) every 14 days for three times. During the study, ambient temperature at the hottest period daily in the MF barn was significantly lower, while relative humidity was higher than that of the NS barn. The temperature humidity index (THI) in both barns ranged from 79-85 throughout the periods of study. Cows in the MF barn showed a lower rectal temperature and respiration rate as compared with cows in the NS barn. The effect of rbST administration increased both rectal temperature and respiration rates of cows housed in either the NS or MF barn. Milk yield significantly increased in cows treated with rbST in all stages of lactation. Increases in mammary blood flow, accompanied by increases of total body water (TBW), extracellular fluid (ECF), blood volume (BV) and plasma volume (PV), were observed in both groups of cows receiving rbST in all stages of lactation. No alterations of renal blood flow and glomerular filtration rate were observed in cows receiving rbST, but decreases in urinary excretion and fractional excretion of sodium, potassium and chloride ions appeared to correlate with reduction in the rate of urine flow and osmolar clearance during rbST administration. These results suggest that the effect of rbST supplementation to cows housed either in NS or MF barns on body fluid volume expansion is attributable to changes in the rate of electrolyte excretion by the kidney. The increased availability of renal tubular reabsorption of sodium, potassium and chloride ions during rbST treatment was a major factor in retaining body water through its colligative properties in exerting formation of an osmotic force mechanism.

• International Journal of Naval Architecture and Ocean Engineering
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• 제5권1호
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• pp.33-46
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• 2013

A computational fluid dynamics (CFD) code, dubbed SNUFOAM, was developed to predict the performance of ship resistance using a CFD tool kit with open source libraries. SNUFOAM is based on a pressure-based cell-centered finite volume method and includes a turbulence model with wall functions. The mesh sensitivity, such as the skewness and aspect ratio, was evaluated for the convergence. Two wall functions were tested to solve the turbulent flow around a ship, and the one without the assumption of the equilibrium state between turbulent production and dissipation in the log law layer was selected. The turbulent flow around a ship simulated using SNUFOAM was compared to that by a commercial CFD code, FLUENT. SNUFOAM showed the nearly same results as FLUENT and proved to be an alternative to commercial CFD codes for the prediction of ship resistance performance.

• 신재생에너지
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• 제2권3호
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• pp.37-41
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• 2006

In this study, an experimental apparatus has been designed and set up to analyze the dissociating phenomena of hydrate in porous rock. Experiments with the depressurization scheme have been carried out to investigate the dissociation characteristics of methane hydrates and the productivities of dissociated gas and water. From the experiments, it has been provided a determination of volume of gas produced and the progress of the dissociation front, as a function of time when hydrate is depressurized. Also, it has been investigated the flowing behavior of the dissociated gas and water in porous rock and the efficiency of the production

• Asian-Australasian Journal of Animal Sciences
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• 제23권5호
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• pp.563-566
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• 2010

Using an open-circuit system, we compared the methane ($CH_4$) emission from dry cows fed first-cut Timothy silage ($1^{st}$ TY), second-cut Timothy silage ($2^{nd}$ TY), second-cut Italian ryegrass silage ($2^{nd}$ IR), third-cut Italian ryegrass silage ($3^{rd}$ IR), or second-cut red clover silage ($2^{nd}$ RC) as the sole feed. The methane emission ranged from 258.2 L $day^{-1}$ to 396.5 L $day^{-1}$. The methane emission from dry cows fed red clover silage was relatively lower than that from dry cows fed grass silage. However, the methane emission per unit digestible neutral detergent fiber (NDF) intake (dNDFI) did not differ significantly between the experimental silages. The methane emission was significantly correlated with the NDF intake and digestibility. Methane emission had a significant correlation with the quadratic function of dNDFI. The differences in the daily volume of methane emission from cows fed different forages can be explained by dNDFI.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권3호
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• pp.189-193
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• 2001

Critical cell density (CCD), the maximum cell concentration without mutual shading in algal cultures, can be used as a new operating parameter for high-density algal cultures and for the application of the flashing light effect on illuminated algal cultures. CCD is a function of average cell volume and light illumination area. The CCD is thus proposed as an index of estimation of mutual shading in algal cultures. Where cell densities are below the CCD, all the cells in photobioreactors can undergo photosysnthesis at their maximum rate. At cell densities over CCD, mutual shading will occur and some cells in the illumination chamber cannot grow photoautotrophically. When the cell concentration is higher than the CCD, specific oxygen production rates under flashing light were higher than those under continuous light. The CCD was found to be a useful engineering parameter for the application of flashing light, particularly in high-density algal cultures.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2006년도 춘계학술대회
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• pp.412-414
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• 2006

In this study, an experimental apparatus has been designed and set up to analyze the dissociating phenomena of hydrate in porous rock. Experiments with the depressurization ion scheme have been carried out to investigate the dissociation characteristics of methane hydrates and the productivities of dissociated gas and water. From the experiments, it has been provided a determination of volume of gas produced and the progress of the dissociation front, as a function of time when hydrate is depressurized. Also, it has been investigated the flowing behavior of the dissociated gas and water in porous rock and the efficiency of the production

• Animal Bioscience
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• 제37권3호
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• pp.500-508
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• 2024

Objective: Due to current selection practices for increased egg production and peak persistency, the production profile, age at maturity, and body weight criteria for commercial layers are constantly changing. Body weight and age at the time of photostimulation will thus always be the factors that need to be adequately addressed among various production systems. The current study was carried out to determine the effects of pullets' body weight (low, medium, and heavy) on their performance, welfare, physiological response, and hormonal profile. Methods: With regard to live weight, 150 16-week-old pullets were divided into three groups using a completely randomized design (CRD) and held until the 50th week. One-way analysis of variance was used to evaluate the data under the CRD, and the least significant difference test was used to distinguish between treatment means. Results: In comparison to the medium and light birds, the heavy birds had higher body weight at maturity, an earlier age at maturity, and higher egg weight, eggshell weight, eggshell thickness, egg yolk index, breaking strength, egg surface area, egg shape index, egg volume, and hormonal profile except corticosterone. However, the medium and light birds had lower feed consumption rates per dozen eggs and per kilogram of egg mass than the heavy birds. Light birds showed greater body weight gain, egg production, and egg specific gravity than the other categories. At 20 weeks of age, physiological response, welfare aspects, and catalase were non-significant; however, at 50 weeks of age, all these factors-aside from catalase-were extremely significant. Conclusion: The findings of this study indicate that layers can function at lower body weights during photostimulation; hence, dietary regimens that result in lighter pullets may be preferable. Additionally, the welfare of the birds was not compromised by the lighter weight group.

• 수산경영론집
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• 제34권2호
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• pp.53-73
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• 2003

This paper is to analyze the past and present consumption pattern of fishes aquacultured in marine waters and thus to draw the policy direction to enhance the competitiveness of marine fish aquaculture in Korea. At present, the volume of meat consumption is surveyed to be more than that of marine fish, but it is revealed that fish consumption will become more increasing in the future according to the rise of the income. The survey shows that the consumption of fish is highest in the fall, and among the various patterns of consumption, live fish, so-called susi, is surveyed to be highly dominant. It is revealed that fish is enjoyed because of the special savor, diverse nutrients, and the prevention of adult diseases. Natural fish Is revealed to be more preferred to aquacultured one due to the sticky flesh quality and the low probability of the remained after the production process antibiotics, so that it is necessary to enhance the taste quality and make a clean cultivation to capture more market demand. Consumption of high-quality fish seems to become high in more than middle class and consumption of fish are estimated to increase in the future, more than that of meat if income level of the people increases. Also, if we try to make our high-quality fish become popular among the public and competible with the imported fish from abroad, it is recommended that they must lower production price by cost reduction and try to differentiate it by taste and environmental safety, etc. It was revealed that the significant factor in demand function for fish is income and it is almost the only factor affecting that demand. Also, it was revealed that the most significant factor affecting preference of fish is income and it Is almost the only factor affecting the preference. Therefore, we can ascertain that if proper goods can be distributed, demand for and preference of fish may increase according to the increase of income in the future.