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Expression of Human Growth Hormone Gene using Retrovirus Vector System In Vitro  

Kim, Min-Ki (Department of Physiology, Catholic University of Daegu School of Medicine)
Koo, Bon-Chul (Department of Physiology, Catholic University of Daegu School of Medicine)
Kwon, Mo-Sun (Department of Physiology, Catholic University of Daegu School of Medicine)
Kim, Te-Oan (Department of Physiology, Catholic University of Daegu School of Medicine)
Publication Information
Reproductive and Developmental Biology / v.35, no.3, 2011 , pp. 251-256 More about this Journal
Abstract
Human growth hormone (hGH), one of the most important hormones in medicine, is secreted from anterior pituitary gland. Its broad physiological function includes body growth, cell regeneration, increasement of muscle volume, bone density, body fat reduction, and so on. Due to the wide range of therapeutic effects, the hGH produced from E. coli has been commercialized already. In this study, we asked whether it is possible to produce recombinant hGH efficiently from various cultured mammalia cells. To meet this purpose, we chose a retrovirus vector system for transfer and expression of the hGH gene in various mammalian cells. Analyses of RT-PCR, ELISA, and Western blot to determine expression of the hGH gene showed the highest production of the hGH was determined from chicken embronic fibroblast (CEF) cells with the concentration of 8.58 ${\mu}g$/ml. The biological activity of the hGH was similar to the commercially available counterpart. These results suggest that mass production of hGH is possible not only in the E. coli but also in the various mammalian cells.
Keywords
hGH; Mammalian cell; Retrovirus vector; Biological activity;
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