• Journal of Korean Academy of Nursing Administration
• /
• v.9 no.2
• /
• pp.283-295
• /
• 2003

This study describes the development of nursing education program on web-based instruction for Nursing Management Practice of nursing school. The program has been developed based on five steps of the teaching-learning design model developed by Chung, In-Sung which is widely used in Web-Based Instruction(WBI). The objective of this study was to develop nursing education program on WEI and to evaluate its effectiveness. The subjects consisted of twenty-four senior nursing students taking the Nursing Management Practice course. Two sets of questionnaires were used for this study. They were asked to evaluate the program in terms of appropriateness of the teaching strategy, precision of the contents, easiness of use, easiness of approaching system, interface design, system management, enhancement of communication, and effectiveness of learning. The program was developed in five steps, analysis of the student, design of the contents, production of the system, implementation of the system, and evaluation of the effectiveness. It is concluded that this program was very useful in increasing the effectiveness of learning and motivation in the students. In addition to this, the web-based learning system would be one of the best qualified educational method for nursing students. Therefore, I would suggest that it can be used for other nursing courses which is available on the Web.

• Asian-Australasian Journal of Animal Sciences
• /
• v.23 no.1
• /
• pp.7-12
• /
• 2010

The full-length cDNA of the porcine peroxisomal ${\Delta}^3$,${\Delta}^2$-enoyl-CoA isomerase (PECI) gene encodes a monofunctional peroxisomal ${\Delta}^3$,${\Delta}^2$-enoyl-CoA isomerase. Cloning and sequencing of the porcine PECI cDNA revealed the presence of an 1185-base pair open reading frame predicted to encode a 394-amino acid protein by the 5'rapid amplification of cDNA ends (5'RACE) and EST sequences. The porcine PECI gene was expressed in seven tissues (heart, liver, spleen, lung, kidney, skeletal muscle, fat) which was revealed by reverse transcriptase-polymerase chain reaction (RT-PCR). The porcine PECI was mapped to SSC71/2 p11-13 using the somatic cell hybrid panel (SCHP) and the radiation hybrid panel (RH) (LOD score 12.84). The data showed that PECI was closely linked to marker S0383. A C/T single nucleotide polymorphism in PECI exon 10 (3'UTR) was detected as a PvuII PCR-RFLP. Association analysis in our experimental pig population showed that different genotypes of PECI gene were significantly associated with the Average Backfat thickness (ABF) (p<0.05) and Buttock backfat thickness (p<0.01).

• Journal of Korea Multimedia Society
• /
• v.8 no.3
• /
• pp.389-397
• /
• 2005

In this paper, we study the synchronization method of animated simulations and VRs for on-line virtual reality education system. To build the data of animated simulations and synchronization modules of VRML content, the media-synchronizing technology, HTML + TIME and scripts are used. The VRML control module ismade into Java applet using the Java EAI provided for the web and the interface for VRML contents. To control the animation of VRML contents, the node information is extracted, and an animation production template capable of controlling animation in inter-connection with VRML content is made. With evaluation of the animation control performance, processing speed, synchronization performance between media in the system built, more improved results are obtained. We would like to suggest a education system with easy system construction and various multi-medium applicability by realizing HTML + TIME media synchronization and VRML web 3D virtual reality creation techniques, and animated images and 3D animation controls according to time using Java through this study.

• Journal of The Korean Association of Information Education
• /
• v.14 no.2
• /
• pp.241-251
• /
• 2010

In knowledge-based society, various forms of visible and invisible works are produced and published. As production and distribution of those works are increased, importance and value of copyright are gradually emerged. In the light of importance, students must learn and understand the concept and the right use of copyright as early as possible. Scales that students experience in the introduction stage of the class take the role of Advance Organizer. Through these scales, students can combine concept of copyright into their existing recognition structure and are interested in study contents. According to test results, it is concluded that the proposed system is very useful to enhance copyright consciousness for students.

• Archives of design research
• /
• v.16 no.4
• /
• pp.335-344
• /
• 2003

Creativity has become a buzzword in the 21s1 century to the extent that it is considered as one of the criteria for a nation's competitiveness. In the cultural industry, one of the industries with the highest growth potential, creativity is the decisive factor Nevertheless, we have to admit that there is a serious lack of this quality in the planning area. Everyone is born with creativity and creative way of looking at things can be developed through training and education. Most of universities now classify digital contents within the realm of design, therefore, emphasizing only the formal or the artistic side of it. However, the study of digital contents requires creative thinking processes that are oriented to problem-solving, for which one needs to put together his/her planning (socio-cultural), expressive (artistic) and technological (scientific) capabilities at the same time. Also, the education of digital contents should be focused not only on acquiring the knowledge and skills, but also on developing individual creativity and learning to increase one's creativity working in a team of an organization. For the environmental aspect, education for creativity should take into account social and cultural specificities of Korea. Therefore, continuous studies must be done to explore more concrete ways of developing creativity on the individual, organizational and environmental levels.

• Journal of Korean Public Health Nursing
• /
• v.14 no.1
• /
• pp.147-160
• /
• 2000

This study was carried out to investigate awareness and attitude of monitors in 206 small sized industries in Seoul and Kyeonggi area toward the occupational health programs using a specially prepared questionnaire. The industries had been running Government-Funded Subsidiary Program since 1998. The questionnaire was designed to be self-administered by respondents. Data were collected for two months from November to December, 1998. Major results are as follows: 1. Between two groups of industries, there were no significant differences in classification, scale, methods of production, existence of trade union, but in type of working pattern and facility ownership there were significant differences. 2. Between two groups of monitors, there were no significant differences in gender, age, marital status, rank, years of education, but in duration of working there was a significant difference. 3. In awareness and attitude of monitors toward their activities and occupational health programs, there was no significant difference by groups of industries, but a significant difference by type and scale of industry, gender, age, years of education 4. It was found that newly subsidized industries were more necessary to provide education programs on occupational health than old industries, and there was a significant difference between two groups of industries in the contents of education needed. In conclusion, it is desirable that the government should develop specific training programs for monitors on occupational health to provide them practical knowledge and specific skills which monitors can apply to their specific situation, and implement the training programs and continue Government-Funded Subsidiary Program for years until small scale industries would have been able to develop and keep their own health management system spontaneously.

• Journal of Microbiology and Biotechnology
• /
• v.24 no.5
• /
• pp.619-628
• /
• 2014

To identify lipase LipA (PFL_0617) from Pseudomonas protegens Pf-5, a lipA deletion mutant (Pf0617) and a complementary strain (Pf0617lipA) were constructed, and their effects on the lipase production were examined. Pf0617 remarkably decreased its whole-cell lipase activity, whereas Pf0617lipA made its whole-cell lipase activity not only restore to wild-type level but also get a further increment. However, the deletion and overexpression of lipA did not affect the extracellular lipase activity. In addition, the unbroken whole cells of these strains were able to catalyze the hydrolysis of membrane-permeable p-nitrophenyl esters, but could not hydrolyze the membrane-impermeable olive oil. These results confirmed that LipA was an intracellular lipase and Pf-5 could also be used as a natural whole-cell biocatalyst. To evaluate the potential of Pf-5 as a whole-cell biocatalyst and separately characterize the whole-cell LipA, the properties of the whole-cell lipases from Pf-5 and Top10lipA were characterized. The results demonstrated that both Pf-5 and Top10lipA exhibited high tolerance to alkaline condition, high temperature, heavy metal ions, surfactants, and organic solvents. Taken together, lipA can realize functional expression in E. coli Top10, and Pf-5 and Top10lipA as whole-cell biocatalysts may have enormous potential in applications.

• Asian-Australasian Journal of Animal Sciences
• /
• v.20 no.10
• /
• pp.1473-1477
• /
• 2007

Four-and-a-half LIM-only protein 3 (FHL3) is a member of the LIM protein superfamily and can participate in mediating protein-protein interaction by binding one another through their LIM domains. In this study, the 5'- and 3'- cDNA ends were characterized by RACE (Rapid Amplification of the cDNA Ends) methodology in combination with in silico cloning based on the partial cDNA sequence obtained. Bioinformatics analysis showed FHL3 protein contained four LIM domains and four LIM zinc-binding domains. In silico mapping assigned this gene to the gene cluster MTF1-INPP5B-SF3A3-FHL3-CGI-94 on pig chromosome 6 where several QTL affecting intramuscular fat and eye muscle area had previously been identified. Transcription of the FHL3 gene was detected in spleen, liver, kidney, small intestine, skeletal muscle, fat and stomach, with the greatest expression in skeletal muscle. The A/G polymorphism in exon II was significantly associated with birth weight, average daily gain before weaning, drip loss rate, water holding capacity and intramuscular fat in a Landrace-derived pig population. Together, the present study provided the useful information for further studies to determine the roles of FHL3 gene in the regulation of skeletal muscle cell growth and differentiation in pigs.

• Journal of Microbiology and Biotechnology
• /
• v.28 no.5
• /
• pp.739-747
• /
• 2018

The low solubility and high-cost recovery of Propionibacterium acnes polyunsaturated fatty acid isomerase (PAI) are key problems in the bioproduction of high value-added conjugated linoleic acid (CLA). To improve the solubility of recombinant PAI, six chaperone proteins were coexpressed with PAI. Introduction of GroELS proteins dramatically improved the PAI solubility from 29% to 97%, with increased activity by 57.8%. Combined expression of DnaKJ-GrpE and GroELS proteins increased the activity by 11.9%. In contrast, coexpression of DnaKJ-GrpE proteins significantly reduced the activity by 57.4%. Plasmids pTf16 harboring the tig gene and pG-Tf2 containing the tig and groEL-groES genes had no visible impact on PAI expression. The lytic protein E was then introduced into the recombinant Escherichia coli to develop a cell autolysis system. A 35% activity of total intracellular PAI was released from the cytoplasm by suspending the lysed cells in distilled water. The PAI recovery was further improved to 81% by optimizing the release conditions. The lipase from Rhizopus oryzae was also expressed in E. coli, with an extracellular activity of 110.9 U/ml. By using the free PAI and lipase as catalysts, a joint system was established for producing CLA from sunflower oil. Under the optimized conditions, the maximum titer of t-10,c-12-CLA reached 9.4 g/l. This work provides an effective and low-cost strategy to improve the solubility and recovery of the recombinant intracellular PAI for further large-scale production of CLA.

• Asian-Australasian Journal of Animal Sciences
• /
• v.25 no.5
• /
• pp.648-652
• /
• 2012

This study was conducted to determine the effects of dietary addition of tea saponins (TS) and soybean oil (SO) on fatty acid profile and gene expression of stearoyl-CoA desaturase (SCD) in longissimus dorsi (LD) muscle of growing lambs. Thirty-two Huzhou lambs were assigned to four dietary treatments in a $2{\times}2$ factorial arrangement with main effects of TS (0 or 3 g/d) and SO (0 or 30 g/kg of diet DM). The diet without additives was considered as NTNS (no TS or SO). After a feeding trial for 60 d, four lambs of each treatment were slaughtered to collect the samples of LD muscle. Percentage of trans-11 vaccenic acid was enhanced (p<0.05) in muscle of lambs fed TS and SO. The proportion of total conjugated linoleic acid (CLA) was increased (p<0.05) by SO, but decreased (p<0.05) by TS in LD muscle. The percentage of total saturated fatty acids in muscle was decreased (p<0.05) by addition of TS and SO, while addition of SO increased (p<0.05) the percentage of total polyunsaturated fatty acids. The ratio of cis-9, trans-11 CLA to tran-11 vaccenic acid was decreased (p<0.05) by TS, but increased (p<0.05) by SO. The same effects were observed in SCD mRNA expression. From these results it is indicated that including TS and SO in the diet of growing lambs affect the fatty acid profiles of LD muscle and that the proportion of cis-9, trans-11 CLA in the muscle influenced by TS and SO may be related to the SCD gene expression.